Nafion修饰碳纤维微电极在抗坏血酸共存下选择性测定去甲肾上腺素
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摘要
制备了碳纤维微电极,将洁净的碳纤维微电极浸入Nafion溶液中,采用电沉积的方法制得Nafion修饰碳纤维微电极。采用循环伏安法(CV)、差分脉冲伏安法(DPV)研究了去甲肾上腺素(NE)和抗坏血酸(AA)在电极上的电化学行为。结果表明:在最优条件下制备的Nafion修饰电极能完全屏蔽AA的电化学响应,而对NE仍表现出良好的电化学响应。修饰电极能在1.0 mmol/L AA的共存下选择性地测定NE,采用DPV进行检测,NE的氧化峰电流与其浓度在1.0×10~(-6)~1.0×10~(-4)mol/L范围内呈良好的线性关系,相关系数(r~2)为0.991 2,检出限(S/N=3)为8.6×10~(-7)mol/L。利用该方法测定了模拟样品中NE的含量,平均加标回收率为101.6%。该电极的重现性和稳定性良好,且具有良好的灵敏度和选择性,有望用于复杂生物环境中NE浓度的检测。
A carbon fiber microelectrode modified with Nafion was fabricated by electrodeposition method on the basis of previous work. The electrochemical behaviour of norepinephrine( NE) and ascorbic acid( AA) at the prepared microelectrodes before and after modification were investigated using cyclic voltammetry( CV) and differential pulse votammetric( DPV) methods. Under the optimum conditions,the Nafion modified electrode can completely shield the electrochemical response of AA,however,a sensitive response to NE was obtained on the modified electrode. Based on this, the modified electrode can be used to selectively detect NE without interference from high concentration AA. DPV response of NE showed a good linearity( r~2= 0. 991 2) at the modified microelectrode with its concentration in the range of 1. 0 × 10~(-6)- 1. 0 × 10~(-4)mol / L,with a detection limit( S / N = 3) of8. 6 × 10~(-7)mol / L. The proposed method was applied in the determination of NE in simulation sample with spiked recovery of 101. 6%. The modified electrode exhibited almost the same electrochemical behavior after a month,indicating that the modified electrode is pretty stable and has a good reproducibility. The advantages of excellent sensitivity and selectivity made the modified electrode could be used to monitor NE in complex biological environment.
A carbon fiber microelectrode modified with Nafion was fabricated by electrodeposition method on the basis of previous work. The electrochemical behaviour of norepinephrine( NE) and ascorbic acid( AA) at the prepared microelectrodes before and after modification were investigated using cyclic voltammetry( CV) and differential pulse votammetric( DPV) methods. Under the optimum conditions,the Nafion modified electrode can completely shield the electrochemical response of AA,however,a sensitive response to NE was obtained on the modified electrode. Based on this, the modified electrode can be used to selectively detect NE without interference from high concentration AA. DPV response of NE showed a good linearity( r~2= 0. 991 2) at the modified microelectrode with its concentration in the range of 1. 0 × 10~(-6)- 1. 0 × 10~(-4)mol / L,with a detection limit( S / N = 3) of8. 6 × 10~(-7)mol / L. The proposed method was applied in the determination of NE in simulation sample with spiked recovery of 101. 6%. The modified electrode exhibited almost the same electrochemical behavior after a month,indicating that the modified electrode is pretty stable and has a good reproducibility. The advantages of excellent sensitivity and selectivity made the modified electrode could be used to monitor NE in complex biological environment.
引文
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[7]Zhang H,Jin B K.Chin.J.Anal.Chem.(张宏,金葆康.分析化学),2002,30(11):1285-1288.
[8]Jin L T,Cui S Y,Shi Z L,Fang Y F.Metall.Anal.,1990,10(5):38-42.
[9]Tan X C,Mai Z B,Wei D P,Deng G H,Huang Z Y,Cai P X.Chin.J.Anal.Chem.(谭学才,麦智彬,韦冬萍,邓光辉,黄在银,蔡沛祥.分析化学),2007,35(4):495-499.
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