广西‘金都一号’火龙果组培技术
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摘要
以‘金都一号’火龙果新萌芽的茎段为外植体,进行组培技术研究,以建立‘金都一号’火龙果组培快繁体系。结果表明:外植体最佳消毒组合为75%酒精20s+0.1%升汞10～15 min,污染率55.70%;初代培养基为MS+6-BA 8.0 mg/L+NAA 0.1 mg/L,诱导率为85.47%;增殖培养基为MS+6-BA 2.0 mg/L,增殖系数为7.13;生根培养基为MS+ABT60.5 mg/L+IBA 0.5 mg/L+NAA 0.1 mg/L,生根率达到98.56%,苗壮根粗,分枝少。
Segments of newly sprouted stem of pitaya 'Jindu No. 1'(Hylocereus undatus) were used as explants for tissue culture, based on which a rapid propagation system was established. The result showed that the best disinfection combination for the explants was 75% alcohol 20 s + 0.1% mercury chloride 10-15 min and had a contamination rate of 55.70 %. The primary culture medium was MS +6-BA 8.0 mg/L +NAA 0.1 mg/L, and the induction rate was 85.47 %. The proliferating medium was MS +6-BA 2.0 mg/L with a proliferation coefficient of 7.13. The rooting medium was MS + ABT60.5 mg/L + IBA 0.5 mg/L+NAA 0.1 mg/L, the rooting rate was 98.56 %, and the plantlets had thick roots and few branches.
Segments of newly sprouted stem of pitaya 'Jindu No. 1'(Hylocereus undatus) were used as explants for tissue culture, based on which a rapid propagation system was established. The result showed that the best disinfection combination for the explants was 75% alcohol 20 s + 0.1% mercury chloride 10-15 min and had a contamination rate of 55.70 %. The primary culture medium was MS +6-BA 8.0 mg/L +NAA 0.1 mg/L, and the induction rate was 85.47 %. The proliferating medium was MS +6-BA 2.0 mg/L with a proliferation coefficient of 7.13. The rooting medium was MS + ABT60.5 mg/L + IBA 0.5 mg/L+NAA 0.1 mg/L, the rooting rate was 98.56 %, and the plantlets had thick roots and few branches.
引文
[1] 1959-2004中国科学院《中国植物志》编委会.中国植物志[M].北京:科学出版社,1999:283-284.
[2]王彬,郑伟,韦茜,等.火龙果的保健价值及发展前景[J].广西热带农业, 2004(3):19-21.
[3]黎舒.火龙果不同品系品种植物学形态和生物学特性研究[D].南宁:广西大学, 2014.
[4]黄青峰.火龙果的棱片离体培养[J].福建农业学报,2002, 17(3):186-189.
[5]黄青峰,余成章.火龙果的组织培养与快速繁殖[J].植物生理学通讯,2002, 38(5):452.
[6]周传明,黄寿先,熊英,等.火龙果茎段离体培养快速繁殖试验[J].广西科学, 2002, 9(1):78-80.
[7]林加耕,张树河.火龙果茎段组织培养和快繁试验[J].福建果树, 2002, 119:6-7.
[8]陈广超,谢晓明,林燕绒.火龙果组培快繁技术[J].中国南方果树, 2003, 32(3):31.
[9]杨红超,郑爱珍,汤正辉.火龙果快速繁殖技术研究[J].北方园艺, 2011(5):167-169.
[10]张领,赵佐敏,陈红艳,等.火龙果离体快繁与组培苗移栽驯化技术[J].贵州农业科学, 2016, 44(2):20-23.
[11]黄文静,金俊军,高凡,等.火龙果成熟胚组培技术研究[J].中国南方果树,2013, 42(1):74-76.
[12]谢志亮,吴振旺,增光辉.白玉龙火龙果成熟种子无菌萌发及组培技术研究[J].中国南方果树, 2016,45(1):58-61.
[13]黄春华,杨健荣,梁秋玲,等.“蜜宝”火龙果的组织培养技术[J].热带农业工程, 2013, 37(3):31-33.
[14]余慧,张伟,朱一仪.红仙蜜火龙果茎段离体快繁技术研究[J].安徽农业科学,2009, 37(9):3 951-3 952.
[15]谢志亮,吴振旺.火龙果生物学特性与品种介绍[J].温州农业科技, 2012(1):42-44.
[16]洪青梅,胡文斌,李洪立,等.‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导[J].热带作物学报, 2017, 38(11):2 119-2 123.
[2]王彬,郑伟,韦茜,等.火龙果的保健价值及发展前景[J].广西热带农业, 2004(3):19-21.
[3]黎舒.火龙果不同品系品种植物学形态和生物学特性研究[D].南宁:广西大学, 2014.
[4]黄青峰.火龙果的棱片离体培养[J].福建农业学报,2002, 17(3):186-189.
[5]黄青峰,余成章.火龙果的组织培养与快速繁殖[J].植物生理学通讯,2002, 38(5):452.
[6]周传明,黄寿先,熊英,等.火龙果茎段离体培养快速繁殖试验[J].广西科学, 2002, 9(1):78-80.
[7]林加耕,张树河.火龙果茎段组织培养和快繁试验[J].福建果树, 2002, 119:6-7.
[8]陈广超,谢晓明,林燕绒.火龙果组培快繁技术[J].中国南方果树, 2003, 32(3):31.
[9]杨红超,郑爱珍,汤正辉.火龙果快速繁殖技术研究[J].北方园艺, 2011(5):167-169.
[10]张领,赵佐敏,陈红艳,等.火龙果离体快繁与组培苗移栽驯化技术[J].贵州农业科学, 2016, 44(2):20-23.
[11]黄文静,金俊军,高凡,等.火龙果成熟胚组培技术研究[J].中国南方果树,2013, 42(1):74-76.
[12]谢志亮,吴振旺,增光辉.白玉龙火龙果成熟种子无菌萌发及组培技术研究[J].中国南方果树, 2016,45(1):58-61.
[13]黄春华,杨健荣,梁秋玲,等.“蜜宝”火龙果的组织培养技术[J].热带农业工程, 2013, 37(3):31-33.
[14]余慧,张伟,朱一仪.红仙蜜火龙果茎段离体快繁技术研究[J].安徽农业科学,2009, 37(9):3 951-3 952.
[15]谢志亮,吴振旺.火龙果生物学特性与品种介绍[J].温州农业科技, 2012(1):42-44.
[16]洪青梅,胡文斌,李洪立,等.‘金都一号’火龙果组织培养之外植体处理优化及不定芽诱导[J].热带作物学报, 2017, 38(11):2 119-2 123.