酸枣仁黄酮部位不同配伍的解郁安神作用及谱-效相关性分析
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摘要
目的建立酸枣仁黄酮部位不同配伍的HPLC指纹图谱,研究其与解郁安神功效的谱效关系。方法采用Spearman分析、灰色关联度分析、多元回归分析方法将HPLC指纹图谱中各组分特征峰的峰面积与小鼠行为学指标数据相关联。结果酸枣仁黄酮部位HPLC指纹图谱中与药效有较强相关性的成分有5个,分别为维采宁-Ⅱ、牡荆素葡萄糖苷、斯皮诺素、6′″-阿魏酰斯皮诺素、6′″-对羟基苯甲酰斯皮诺素。结论通过谱-效相关性分析,推测出酸枣仁黄酮部位发挥抗抑郁、镇静催眠作用的有效成分,为酸枣仁有效成分筛选及质量控制方法的建立提供了依据。
Objective To establish the HPLC fingerprints of different compatibility of flavonoids in Ziziphi Spinosae Semen(ZSS) so as to establish spectrum-efficient correlation between HPLC fingerprints and the effects of depression-resolving and spirit-tranquilizing. Methods Spearman analysis, grey relational analysis, and multiple regression analysis were used to correlate the peak area of the characteristic peaks of each component in the HPLC fingerprint with the behavior indexes of depressive and insomnia mice. Results There were five chemical compositions strongly related with efficacy of flavonoids in ZSS, including vicenin-Ⅱ, glucosylvitexin, spinosin, 6′″-feruloylspinosin, and 6′″-p-hydroxybenzoylspinosin. Conclusion The effective components of flavonoids in ZSS with depression-resolving and spirit-tranquilizing effects can be inferred by spectrum-efficient correlation analysis, which can provide the basis for the screening and quality control of effective components of ZSS.
Objective To establish the HPLC fingerprints of different compatibility of flavonoids in Ziziphi Spinosae Semen(ZSS) so as to establish spectrum-efficient correlation between HPLC fingerprints and the effects of depression-resolving and spirit-tranquilizing. Methods Spearman analysis, grey relational analysis, and multiple regression analysis were used to correlate the peak area of the characteristic peaks of each component in the HPLC fingerprint with the behavior indexes of depressive and insomnia mice. Results There were five chemical compositions strongly related with efficacy of flavonoids in ZSS, including vicenin-Ⅱ, glucosylvitexin, spinosin, 6′″-feruloylspinosin, and 6′″-p-hydroxybenzoylspinosin. Conclusion The effective components of flavonoids in ZSS with depression-resolving and spirit-tranquilizing effects can be inferred by spectrum-efficient correlation analysis, which can provide the basis for the screening and quality control of effective components of ZSS.
引文
[1]中国药典[S].一部.2015.
[2]袁昌鲁,王中博,焦莹,等.酸枣仁中黄酮类镇静催眠有效成分的研究[J].中药通报,1987,12(9):36-38.
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[5]Porsolt R D,Anton G,Blavet N,et al.Behavioural despair in rats:A new model sensitive to antidepressant treatmeats[J].Eur J Pharmacol,1978,47(4):379-391.
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[8]杨旭,冉瑞雪,宋伟,等.酸枣仁黄酮HPLC指纹图谱建立及2种成分测定[J].中成药,2017,39(5):989-992.
[9]王开军,黄添强.基于趋势秩的Spearman相关方法[J].福建师大学报:自然科学版,2010,26(1):38-41.
[10]田兵,冉雪琴,薛红,等.贵州42种野生牧草营养价值灰色关联度分析[J].草业学报,2014,23(1):92-103.
[11]白秀琴,李瑞阁.多元回归分析方法应用实证分析与比较[J].河南科学,2010,28(9):1083-1088.
[12]陶金华,狄留庆,文红梅,等.中药指纹图谱谱效相关性研究思路探讨[J].中国中药杂志,2009,34(18):2410-2413.
[13]佟晴,袁永胜,徐勤荣,等.帕金森病伴发抑郁患者血浆谷氨酸和γ-氨基丁酸水平的改变及其临床意义[J].中国神经精神疾病杂志,2015,41(4):224-228.
[14]吴玉兰,许惠琴,陈诜.酸枣仁不同炮制品及炒酸枣仁中总黄酮与总皂苷的镇静催眠作用比较[J].时珍国医国药,2005,16(9):868-869.
[2]袁昌鲁,王中博,焦莹,等.酸枣仁中黄酮类镇静催眠有效成分的研究[J].中药通报,1987,12(9):36-38.
[3]赵启铎,舒乐新,王颖,等.酸枣仁总黄酮抗抑郁作用的实验研究[J].天津中医药,2011,28(4):335-337.
[4]任燕萍.酸枣仁有效成分的提取及其药理作用研究[J].海峡药学,2011,23(12):89-91.
[5]Porsolt R D,Anton G,Blavet N,et al.Behavioural despair in rats:A new model sensitive to antidepressant treatmeats[J].Eur J Pharmacol,1978,47(4):379-391.
[6]Steru L,Chermat R,Thierry B,et al.The tail suspension test:A new method for screening antidepressants in mice[J].Psychopharmacology,1985,85(3):367-370.
[7]陈奇.中国药理研究方法学[M].北京:人民卫生出版社,1993.
[8]杨旭,冉瑞雪,宋伟,等.酸枣仁黄酮HPLC指纹图谱建立及2种成分测定[J].中成药,2017,39(5):989-992.
[9]王开军,黄添强.基于趋势秩的Spearman相关方法[J].福建师大学报:自然科学版,2010,26(1):38-41.
[10]田兵,冉雪琴,薛红,等.贵州42种野生牧草营养价值灰色关联度分析[J].草业学报,2014,23(1):92-103.
[11]白秀琴,李瑞阁.多元回归分析方法应用实证分析与比较[J].河南科学,2010,28(9):1083-1088.
[12]陶金华,狄留庆,文红梅,等.中药指纹图谱谱效相关性研究思路探讨[J].中国中药杂志,2009,34(18):2410-2413.
[13]佟晴,袁永胜,徐勤荣,等.帕金森病伴发抑郁患者血浆谷氨酸和γ-氨基丁酸水平的改变及其临床意义[J].中国神经精神疾病杂志,2015,41(4):224-228.
[14]吴玉兰,许惠琴,陈诜.酸枣仁不同炮制品及炒酸枣仁中总黄酮与总皂苷的镇静催眠作用比较[J].时珍国医国药,2005,16(9):868-869.