短管兔耳草化学成分的研究
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摘要
目的研究短管兔耳草Lagotis brevituba Maxim的化学成分。方法短管兔耳草70%乙醇提取物采用硅胶、Sephadex LH-20、MCI柱和制备液相色谱进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。结果从中分离得到10个化合物,分别鉴定为3,4-二甲氧基苯甲醛(1)、3,4-二甲氧基苯丙酸(2)、阿魏酸甲酯(3)、邻苯二甲酸二丁酯(4)、木犀草素(5)、柯伊利素-7-O-β-D-吡喃葡萄糖苷(6)、香叶木素-7-O-β-D-吡喃葡萄糖苷(7)、4-p-香豆酸-O-α-D-吡喃葡萄糖苷(8)、4-p-香豆酸-O-β-D-吡喃葡萄糖苷(9)、6-O-[α-L-(3-3,4-二甲氧基肉桂酰)-吡喃鼠李糖基]-梓醇(10)。结论化合物2～4、8～10为首次从该属分离得到,化合物1～4、8～10为首次从该植物中分离得到。
AIM To study the chemical constituents from Lagotis brevituba Maxim. METHODS The 70%ethanol extract of L. brevituba was isolated and purified by silica gel,Sephadex LH-20,MCI column and preparative liquid chromatography, then the structures of obtained compounds were identified by physicochemical properties and spectral data. RESULTS Ten compounds were isolated and identified as 3,4-dimethoxybenzaldehyde( 1),3,4-dimethoxy phenylpropionic acid( 2),methyl ferulic acid( 3),dibutyl phthalate( 4),luteolin( 5),chrysoeriol-7-O-β-D-glucoside( 6),diosmetin-7-O-β-D-glucopyranoside( 7),4-p-cumaroyl-O-α-D-glucose( 8),4-p-cumar-oyl-O-β-D-glucose( 9),6-O-[α-L-( 3-3,4-dimethoxycinnamoyl)-rhamnopyranosy]-catalpol( 10). CONCLUSION Compounds 2-4、8-10 are isolated from genus Lagotis for the first time,and compounds1-4、8-10 are isolated from this plant for the first time.
AIM To study the chemical constituents from Lagotis brevituba Maxim. METHODS The 70%ethanol extract of L. brevituba was isolated and purified by silica gel,Sephadex LH-20,MCI column and preparative liquid chromatography, then the structures of obtained compounds were identified by physicochemical properties and spectral data. RESULTS Ten compounds were isolated and identified as 3,4-dimethoxybenzaldehyde( 1),3,4-dimethoxy phenylpropionic acid( 2),methyl ferulic acid( 3),dibutyl phthalate( 4),luteolin( 5),chrysoeriol-7-O-β-D-glucoside( 6),diosmetin-7-O-β-D-glucopyranoside( 7),4-p-cumaroyl-O-α-D-glucose( 8),4-p-cumar-oyl-O-β-D-glucose( 9),6-O-[α-L-( 3-3,4-dimethoxycinnamoyl)-rhamnopyranosy]-catalpol( 10). CONCLUSION Compounds 2-4、8-10 are isolated from genus Lagotis for the first time,and compounds1-4、8-10 are isolated from this plant for the first time.
引文
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[5]曾金祥,许兵兵,李敏,等.藏药短管兔耳草醇提物降低急性高尿酸血尿酸症小鼠血到水平及机制研究[J].中国新药杂志,2015,24(21):2489-2493.
[6]杨柏龄,侯茜,胡峰,等.短管兔耳草总黄酮降低阿尔茨海默病模型小鼠大脑皮层及海马组织炎性细胞因子的水平[J].细胞与分子免疫学杂志,2016,32(7):881-885.
[7]朱继孝,张红阳,李雪溦,等.两种兔耳草对四氯化碳致肝损伤小鼠保护作用的比较研究[J].中药新药与临床药理,2016,27(2):210-214.
[8]王燕,张应鹏,马兴铭.短管兔耳草正丁醇提取物诱导胃癌SGC-7901细胞凋亡的研究[J].中成药,2007,29(5):672-675.
[9] Calver D J,Cambie R C,Davis B R.13C-NMR spectra of polymethoxyand methyl enedioxy flavonols[J]. Org Magn Reson,1979,12(10):583-586.
[10] Sun Y,Rao G X. Studies on chemical constituents of yunnan propolis[J]. J Chin Med Mater,2016,39(10):2247-2250.
[11]于民丰,邵赟,陶燕铎.藏药鲜卑花化学成分研究[J].中草药,2014,45(24):3528-3531.
[12]石巍,韩桂秋.款冬花的化学成分[J].中国药学杂志,1996,5(2):63-67.
[13]李明潺,唐生安,段宏泉.野雉尾金粉蕨化学成分研究[J].中草药,2010,41(5):685-688.
[14] Dinda,Biswanath G,Biplab,et al. Basudev chemical constituents of Gomphrena globosa. II[J]. Nat Prod Sci,2006,12(2):89-93.
[15]赵春超,邵建华,曹丹丹,等.蓬子菜化学成分研究[J].中国中药杂志,2009,34(21):2761-2764.
[16]王志芳,符崖,向俊,等.广东凉茶颗粒苷类化学成分研究[J].中山大学学报(自然科学版),2013,52(2):79-83.
[17] Tsutomu W,Toshio M,Akira U. Ridoid Glycosides from Verbascum thapsus L.[J]. Chem Pharm Bull,1991,39(12):3261-3264.