超高效液相色谱法同时测定活血涤痰汤中7种活性成分的含量
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摘要
目的建立UPLC方法同时测定活血涤痰汤中黄芪甲苷、柴胡皂苷a、柴胡皂苷d、大黄酸、大黄素、大黄酚和大黄素甲醚含量。方法采用Waters BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相为乙腈(A)-0.1%磷酸水溶液(B),梯度洗脱,体积流量为0.3 mL·min~(-1),进样量为3μL,检测波长分别为205和254 nm。结果黄芪甲苷、柴胡皂苷a、柴胡皂苷d、大黄酸、大黄素、大黄酚、大黄素甲醚的线性范围分别为2.89～57.76μg·m L~(-1)(r=0.9993)、2.10～42.08μg·m L~(-1)(r=0.9994)、3.04～60.88μg·m L~(-1)(r=0.9993)、1.06～21.12μg·m L~(-1)(r=0.9998)、0.89～17.84μg·m L~(-1)(r=0.9999)、1.18～23.60μg·m L~(-1)(r=0.9999)、1.14～22.88μg·m L~(-1)(r=0.9998),平均加样回收率分别为97.5%,97.3%、98.2%、99.3%、99.1%、99.1%和98.8%,RSD值分别为2.4%、2.5%、2.0%、2.0%、2.1%、2.1%和1.9%。结论建立的UPLC方法简便快速,重现性好,可用于活血涤痰汤的质量控制与评价。
Objective To establish a method to simultaneously determine astragaloside Ⅳ, saikosaponin a, saikosaponin d, rhein, emodin, chrysophanol and physcion in Huoxue Ditan decoction. Methods The determination was performed on a Waters BEH C18 column by gradient elution, with acetonitrile-0.1% phosphoric acid water solution as the mobile phase at the flow rate of 0.3 mL·min-1. The injection volume was 3 μL and the detection wavelength was 205 and 254 nm, respectively. Results Astragaloside Ⅳ, saikosaponin a, saikosaponin d, rhein, emodin, chrysophanol and physcion had a good linearity at 2.89-57.76 μg·m L~(-1)(r = 0.9993), 2.10-42.08 μg·m L~(-1)(r = 0.9994), 3.04-60.88 μg·m L~(-1)(r = 0.9993), 1.06-21.12 μg·m L~(-1)(r = 0.9998), 0.89-17.84 μg·m L~(-1)(r = 0.9999), 1.18-23.60 μg·m L~(-1)(r = 0.9999), and 1.14-22.88 μg·m L~(-1)(r = 0.9998), respectively. The average recovery were 97.5%, 97.3%, 98.2%, 99.3%, 99.1%, 99.1% and 98.8%, with RSDs of 2.4%, 2.5%, 2.0%, 2.0%, 2.1%, 2.1% and 1.9%. Conclusion The UPLC method is simple, rapid and reproducible, which can be used for the quality control and evaluation of Huoxue Ditan decoction.
Objective To establish a method to simultaneously determine astragaloside Ⅳ, saikosaponin a, saikosaponin d, rhein, emodin, chrysophanol and physcion in Huoxue Ditan decoction. Methods The determination was performed on a Waters BEH C18 column by gradient elution, with acetonitrile-0.1% phosphoric acid water solution as the mobile phase at the flow rate of 0.3 mL·min-1. The injection volume was 3 μL and the detection wavelength was 205 and 254 nm, respectively. Results Astragaloside Ⅳ, saikosaponin a, saikosaponin d, rhein, emodin, chrysophanol and physcion had a good linearity at 2.89-57.76 μg·m L~(-1)(r = 0.9993), 2.10-42.08 μg·m L~(-1)(r = 0.9994), 3.04-60.88 μg·m L~(-1)(r = 0.9993), 1.06-21.12 μg·m L~(-1)(r = 0.9998), 0.89-17.84 μg·m L~(-1)(r = 0.9999), 1.18-23.60 μg·m L~(-1)(r = 0.9999), and 1.14-22.88 μg·m L~(-1)(r = 0.9998), respectively. The average recovery were 97.5%, 97.3%, 98.2%, 99.3%, 99.1%, 99.1% and 98.8%, with RSDs of 2.4%, 2.5%, 2.0%, 2.0%, 2.1%, 2.1% and 1.9%. Conclusion The UPLC method is simple, rapid and reproducible, which can be used for the quality control and evaluation of Huoxue Ditan decoction.
引文
[1]李丽娜.涤痰汤合通窍活血汤联合吞咽功能训练对脑梗死后吞咽障碍的疗效观察[J].陕西中医,2016,37(5):526-528.
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[6]罗丹,张晶,赵文娟.黄芪甲苷通过抑制NF-κB活化减轻棕榈酸诱导脂肪细胞炎症因子的表达[J].中南药学,2017,15(12):1721-1724.
[7]马春艳,陈雨微,黄家君,等.大黄素和芹菜素对高血压大鼠的肾脏保护及抗氧化作用[J].中药药理与临床,2018,34(4):69-73.
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[9]董海影,张静艳,柏青杨,等.柴胡皂苷A对抑郁模型大鼠海马神经细胞凋亡的保护作用[J].中国老年学杂志,2015,35(7):1935-1937.
[10]宁艳梅,杨秀娟,杨韬.甘肃产柴胡柴胡皂苷a含量测定及抗抑郁作用评价[J].中兽医医药杂志,2014,33(6):34-37.
[11]张洪峰,王乐,张凯,等.柴胡总皂苷的循环超声提取及其对HepG2细胞凋亡的影响[J].中南药学,2016,14(12):1316-1319.
[2]周艳芳,王继光.活血涤痰汤对高血压脑出血神经功能恢复的影响[J].世界最新医学信息文摘,2017,17(19):91-92.
[3]秦德芳,张泽舜,马丁,等.活血涤痰汤对高血压脑出血(HICH)神经功能恢复影响及作用机制[J].黑龙江医药,2016,29(3):479-480.
[4]张云兴.涤痰汤联合通窍活血汤治疗脑卒中后抑郁症30例[J].云南中医中药杂志,2016,37(6):89-90
[5]李丽丽.活血涤痰汤对高血压脑出血神经功能恢复的影响[J].中国实验方剂学杂志,2015,21(4):189-192.
[6]罗丹,张晶,赵文娟.黄芪甲苷通过抑制NF-κB活化减轻棕榈酸诱导脂肪细胞炎症因子的表达[J].中南药学,2017,15(12):1721-1724.
[7]马春艳,陈雨微,黄家君,等.大黄素和芹菜素对高血压大鼠的肾脏保护及抗氧化作用[J].中药药理与临床,2018,34(4):69-73.
[8]于立杰,李凤丹,肖荷妹,等.大黄麻黄方对大鼠血压的疗效及作用机制研究[J].中国地方病防治杂志,2018,33(4):441-442,444.
[9]董海影,张静艳,柏青杨,等.柴胡皂苷A对抑郁模型大鼠海马神经细胞凋亡的保护作用[J].中国老年学杂志,2015,35(7):1935-1937.
[10]宁艳梅,杨秀娟,杨韬.甘肃产柴胡柴胡皂苷a含量测定及抗抑郁作用评价[J].中兽医医药杂志,2014,33(6):34-37.
[11]张洪峰,王乐,张凯,等.柴胡总皂苷的循环超声提取及其对HepG2细胞凋亡的影响[J].中南药学,2016,14(12):1316-1319.