藏红花素对糖尿病所致睾丸组织损伤保护作用的研究
|
摘要
[目的]研究藏红花素(Crocin)对实验性2型糖尿病大鼠睾丸组织并发症损伤的保护作用。[方法] 100只SD大鼠按体质量随机分为正常对照组、模型对照组、藏红花素25 mg/(kg·d)、50 mg/(kg·d)、100 mg/(kg·d)组,每组20只,灌胃给药,疗程12周;采用链脲佐菌素(STZ)连续3 d腹腔注射30 mg/(kg·d)的方法制备实验性2型糖尿病大鼠模型。测定各组大鼠空腹血糖(FBG)、空腹胰岛素(FINS)和糖化血红蛋白(GHb)水平,称量大鼠体质量、睾丸质量后计算睾丸指数,测定血清睾酮含量;采用苏木精-伊红(HE)染色对睾丸组织进行病理学检查,通过透射电镜观察睾丸组织超微结构改变;末端脱氧核苷酰基转移酶介导dUTP切口末端标记法(TUNEL)观察睾丸组织细胞凋亡状况。[结果]经藏红花素50～100 mg/(kg·d)治疗12周,2型糖尿病大鼠FBG显著降低、FINS显著升高、GHb显著降低(P<0.05或P<0.01);体质量、睾丸指数和血清睾酮含量显著升高(P<0.05或P<0.01)。经藏红花素治疗12周,糖尿病大鼠睾丸组织曲细精管萎缩、生精细胞脱落、生精细胞数量减少等病理学改变以及细胞核固缩、染色质块状浓集、线粒体减少等超微结构改变均明显改善;睾丸组织凋亡细胞数量明显减少,细胞凋亡指数(AI)显著降低(P<0.05或P<0.01)。[结论]藏红花素能够有效抑制糖尿病大鼠睾丸组织损伤,其机制可能与其降低血糖作用有关。
[Objective] To investigate the protective effects of crocin on testicular tissue injury caused by diabetes mellitus. [Methods]The 100 SD rats were randomized according to body weight divided into normal control group,model group,Crocin [25,50,100 mg/(kg·d)] groups,the drugs were given by gastric administration for 12 weeks. The diabetic rat models were made by intraperitoneal injecting STZ [30 mg/(kg·d)] for 3 d continuously. The general condition of the rats in every group was observed;the FBG,FINS,GHb were detected. The body weight was detected and the testis tissue index was calculated,the content of testosterone in serum was determined;the histopathological changes of testis tissue was observed by HE staining,the ultrastructure of testis tissue was observed by electron microscope,the apoptosis of testis tissue apoptosis was observed by TUNEL. [Results] After treatment with crocin 50～100 mg/(kg·d) for12 weeks,the FBG decreased significantly,FINS increased significantly,GHb decreased significantly in type 2 diabetic rat models(P<0.05 or P<0.01);the body weight,the testis tissue index and the content of testosterone were significantly increased(P<0.05 or P<0.01).12 weeks later,the ultrastructural changes such as atrophy of seminiferous tubules,loss of spermatogenic cells and reduction of spermatogenic cells in testis tissue of diabetic rats,and ultrastructural changes such as nuclear condensation,chromatin mass accumulation,and reduction of mitochondria were significantly improved;the number of apoptotic cells in the testis decreased significantly,the Apoptosis Index(AI) decreased significantly(P <0.05 or P <0.01). [Conclusion] Crocin can effectively inhibit testicular tissue damage in diabetic rats,which mechanism may be related to its role in lowering blood sugar.
[Objective] To investigate the protective effects of crocin on testicular tissue injury caused by diabetes mellitus. [Methods]The 100 SD rats were randomized according to body weight divided into normal control group,model group,Crocin [25,50,100 mg/(kg·d)] groups,the drugs were given by gastric administration for 12 weeks. The diabetic rat models were made by intraperitoneal injecting STZ [30 mg/(kg·d)] for 3 d continuously. The general condition of the rats in every group was observed;the FBG,FINS,GHb were detected. The body weight was detected and the testis tissue index was calculated,the content of testosterone in serum was determined;the histopathological changes of testis tissue was observed by HE staining,the ultrastructure of testis tissue was observed by electron microscope,the apoptosis of testis tissue apoptosis was observed by TUNEL. [Results] After treatment with crocin 50～100 mg/(kg·d) for12 weeks,the FBG decreased significantly,FINS increased significantly,GHb decreased significantly in type 2 diabetic rat models(P<0.05 or P<0.01);the body weight,the testis tissue index and the content of testosterone were significantly increased(P<0.05 or P<0.01).12 weeks later,the ultrastructural changes such as atrophy of seminiferous tubules,loss of spermatogenic cells and reduction of spermatogenic cells in testis tissue of diabetic rats,and ultrastructural changes such as nuclear condensation,chromatin mass accumulation,and reduction of mitochondria were significantly improved;the number of apoptotic cells in the testis decreased significantly,the Apoptosis Index(AI) decreased significantly(P <0.05 or P <0.01). [Conclusion] Crocin can effectively inhibit testicular tissue damage in diabetic rats,which mechanism may be related to its role in lowering blood sugar.
引文
[1]武向丽.黄芪甲苷对实验性糖尿病大鼠睾丸组织的保护作用[J].西部中医药,2017,30(11):32-36.
[2]Elsherbiny NM,Salama MF,Said E,et al.Crocin protects against doxorubicin-induced myocardial toxicity in rats through downregulation of inflammatory and apoptic pathways[J].Chem Biol Interact,2016,247:39-48.
[3]吕明锐,王倩然,杨升东,等.藏红花及其组分抑制炎症因子表达的研究进展[J].重庆医学,2017,46(13):1850-1853.
[4]李赛,杨慧科,丁选胜.藏红花素对糖尿病大鼠胸主动脉环舒缩功能的影响[J].药学与临床研究,2017,25(3):183-186.
[5]郭斌,钦华,王莉,等.藏红花素对糖基化终产物诱导视网膜微血管内皮细胞凋亡的影响[J].医学研究生学报,2012,25(11):1141-1145.
[6]王素莲,黄伟,熊玉卿,等.藏红花降血脂作用的研究进展[J].中国临床药理学杂志,2015,31(12):1218-1220.
[7]赵茜,李红梅,常越,等.氟伐他汀对糖尿病大鼠睾丸组织氧化应激水平的影响[J].宁夏医学杂志,2015,37(8):673-674.
[8]曹雯,郑仁东,范尧夫,等.2型糖尿病男性患者睾酮水平与代谢指标相关性分析[J].四川大学学报(医学版),2018,49(2):235-238.
[9]张晓艳,刘明明,李炳蔚,等.观察链脲佐菌素诱导的BALB/c糖尿病小鼠睾丸微循环损害[J].微循环学杂志,2015,25(3):1-4.
[10]李徽徽,于影,贺文欣,等.乙醇激活ALDH2对2型糖尿病大鼠睾丸损伤的影响[J].中国药理学通报,2015,31(7):962-966.
[11]阚敏宸.山楂叶总黄酮对2型糖尿病大鼠睾丸组织病变的改善作用[J].现代中西医结合杂志,2016,25(18):1957-1960.
[12]毛孙忠,陈国荣,雷康福,等.银杏叶提取物对糖尿病大鼠睾丸损伤的作用[J].中国病理生理杂志,2004,20(7):1238-1242.
[2]Elsherbiny NM,Salama MF,Said E,et al.Crocin protects against doxorubicin-induced myocardial toxicity in rats through downregulation of inflammatory and apoptic pathways[J].Chem Biol Interact,2016,247:39-48.
[3]吕明锐,王倩然,杨升东,等.藏红花及其组分抑制炎症因子表达的研究进展[J].重庆医学,2017,46(13):1850-1853.
[4]李赛,杨慧科,丁选胜.藏红花素对糖尿病大鼠胸主动脉环舒缩功能的影响[J].药学与临床研究,2017,25(3):183-186.
[5]郭斌,钦华,王莉,等.藏红花素对糖基化终产物诱导视网膜微血管内皮细胞凋亡的影响[J].医学研究生学报,2012,25(11):1141-1145.
[6]王素莲,黄伟,熊玉卿,等.藏红花降血脂作用的研究进展[J].中国临床药理学杂志,2015,31(12):1218-1220.
[7]赵茜,李红梅,常越,等.氟伐他汀对糖尿病大鼠睾丸组织氧化应激水平的影响[J].宁夏医学杂志,2015,37(8):673-674.
[8]曹雯,郑仁东,范尧夫,等.2型糖尿病男性患者睾酮水平与代谢指标相关性分析[J].四川大学学报(医学版),2018,49(2):235-238.
[9]张晓艳,刘明明,李炳蔚,等.观察链脲佐菌素诱导的BALB/c糖尿病小鼠睾丸微循环损害[J].微循环学杂志,2015,25(3):1-4.
[10]李徽徽,于影,贺文欣,等.乙醇激活ALDH2对2型糖尿病大鼠睾丸损伤的影响[J].中国药理学通报,2015,31(7):962-966.
[11]阚敏宸.山楂叶总黄酮对2型糖尿病大鼠睾丸组织病变的改善作用[J].现代中西医结合杂志,2016,25(18):1957-1960.
[12]毛孙忠,陈国荣,雷康福,等.银杏叶提取物对糖尿病大鼠睾丸损伤的作用[J].中国病理生理杂志,2004,20(7):1238-1242.