不同施硒方式对水稻硒利用效率的影响
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摘要
采用盆栽试验方法,研究土壤施硒和叶面喷硒两种施硒方式(均为硒酸钠形态)对水稻籽粒硒生物强化以及营养品质的影响机制。结果表明:等量施硒条件下,土壤施硒和叶面施硒两种方式均能显著提高水稻地上部和籽粒硒含量,土壤施硒水稻地上部和籽粒硒含量分别为叶面喷硒处理的8.9倍和5.3倍,说明在等量施硒条件下土壤施硒较叶面施硒更能有效提高籽粒硒含量。叶面喷硒处理的籽粒硒分配系数为土壤施硒的2倍,说明硒通过叶片向籽粒转运速率较通过根系到籽粒的转运速率更快。土壤施硒能够显著提高籽粒中镁、硫、铁、锰和锌等矿质营养元素含量。相比对照组,土壤施硒和叶面喷硒的水稻籽粒粗蛋白分别提高8%和4.5%,丝氨酸和酪氨酸含量说明施硒能够提高水稻营养品质,以土壤施硒效果更佳。
【Objective】 Application of Se fertilizer is an important agronomic rice Se biofortification measure, and is usually done in two ways, i.e. foliar spray(FS) and application in soil(SA). However,as affected by the way of Se application, Se absorption and translocation of Se into rice grain varies in mechanism. Therefore, it is of great practical significance to study the differences between the two Se application methods in effect on Se absorption and translocation and nutritional quality of rice. 【Method】A pot experiment was conducted to study mechanisms of FS and SA affecting Se biofortification and nutritional quality of rice grains. 【Result】 Results show that the two methods did not vary much in effect on rice growth. The same in Se application rate, the two methods both increased Se content in rice shoot and grain significantly. However, the content of Se in rice shoot and grain was 8.9 and 5.3 times as high in SA as in FS, respectively, which indicates that SA could more effectively increase Se content in rice grain than FS. But the distribution coefficient of Se in grain was 2 times higher in FS than in SA, which demonstrates that the mechanism of transporting Se from leaf into grain was more effective than that from root to grain.However, no significant effect on Se form in rice grains relative to Se application method, which indicates that Se application methods affected only transport, rather than assimilation of Se in rice. SA could significantly increase the contents of magnesium(Mg), sulfur(S), iron(Fe), manganese(Mn) and zinc(Zn)in rice grain. Compared with the control(No Se applied), SA and FS was 8% and 4.5% higher in content of crude protein in rice grain, respectively, which shows that Se can improve nutritional quality of rice,and the effect of SA was more significant than that of FS. Meanwhile, Se application can also increase the content of serine(Ser) and tyrosine(Tyr) as compared with CK, and, the effect on Tyr was more significant in SA than that in FS or 1.15 times as higher in the former than in the latter. 【Conclusion】Therefore,considering cost-effectiveness, SA is better than FS in applying selenate, for it improves more significantly Se content and nutritional quality of rice grains.
【Objective】 Application of Se fertilizer is an important agronomic rice Se biofortification measure, and is usually done in two ways, i.e. foliar spray(FS) and application in soil(SA). However,as affected by the way of Se application, Se absorption and translocation of Se into rice grain varies in mechanism. Therefore, it is of great practical significance to study the differences between the two Se application methods in effect on Se absorption and translocation and nutritional quality of rice. 【Method】A pot experiment was conducted to study mechanisms of FS and SA affecting Se biofortification and nutritional quality of rice grains. 【Result】 Results show that the two methods did not vary much in effect on rice growth. The same in Se application rate, the two methods both increased Se content in rice shoot and grain significantly. However, the content of Se in rice shoot and grain was 8.9 and 5.3 times as high in SA as in FS, respectively, which indicates that SA could more effectively increase Se content in rice grain than FS. But the distribution coefficient of Se in grain was 2 times higher in FS than in SA, which demonstrates that the mechanism of transporting Se from leaf into grain was more effective than that from root to grain.However, no significant effect on Se form in rice grains relative to Se application method, which indicates that Se application methods affected only transport, rather than assimilation of Se in rice. SA could significantly increase the contents of magnesium(Mg), sulfur(S), iron(Fe), manganese(Mn) and zinc(Zn)in rice grain. Compared with the control(No Se applied), SA and FS was 8% and 4.5% higher in content of crude protein in rice grain, respectively, which shows that Se can improve nutritional quality of rice,and the effect of SA was more significant than that of FS. Meanwhile, Se application can also increase the content of serine(Ser) and tyrosine(Tyr) as compared with CK, and, the effect on Tyr was more significant in SA than that in FS or 1.15 times as higher in the former than in the latter. 【Conclusion】Therefore,considering cost-effectiveness, SA is better than FS in applying selenate, for it improves more significantly Se content and nutritional quality of rice grains.
引文
[1]Cai X L,Wang C,Yu W Q,et al.Selenium exposure and cancer risk:An updated meta-analysis and metaregression.Scientific Reports,2016(6):Article number 19213
[2]Xia Y M,Hill K E,Byrne D W,et al.Effectiveness of selenium supplements in a low-selenium area of China.The American Journal of Clinical Nutrition,2005,81:829-834
[3]Sharma P,Aggarwal P,Kaur A.Biofortification:A new approach to eradicate hidden hunger.Food Reviews International,2017,33(1):1-21
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[5]周鑫斌,施卫明,杨林章.叶面喷硒对水稻籽粒硒富集及分布的影响.土壤学报,2007,44(1):73-78Zhou X B,Shi W M,Yang L Z.Effect of foliar application of selenite on selenium accumulation and distribution in rice(In Chinese).Acta Pedologica Sinica,2007,44(1):73-78
[6]Sun G X,Liu X,Williams P N,et al.Distribution and translocation of selenium from soil to grain and its speciation in paddy rice(Oryza sativa L.).Environmental Science and Technology,2010,44(17):6706-6711
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[9]El Kassis E,Cathala N,Rouached H,et al.Characterization of a selenate-resistant Arabidopsis mutant.Root growth as a potential target for selenate toxicity.Plant Physiology,2007,143(3):1231-1241
[10]Fujii R,Deverel S J,Hatfield D B.Distribution of selenium in soils of agricultural fields,western San Joaquin Valley,California.Soil Science Society of America Journal,1988,52(5):1274-1283
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[12]Zhang L H,Shi W M,Wang X C.Difference in selenite absorption between high-and low-selenium rice cultivars and its mechanism.Plant and Soil,2006,282(1):183-193
[13]Amagliani L,O’Regan J,Keny A L,et al.Composition and protein profile analysis of rice protein ingredients.Journal of Food Composition and Analysis,2017,59:18-26
[14]中华人民共和国农业部.NY/T83-1988:米质测定方法.北京:中国标准出版社,1988The Ministry of Agriculture of the People’s Republic of China.NY/T83-1988:The standard for the method of the rice quality determination(In Chinese).Beijing:China Standard Press,1988
[15]穆乃耀,唐设,陈文珠,等.氮素粒肥缓解水稻灌浆期高温胁迫的生理机制研究.南京农业大学学报,2017,40(1):1-10Mu N Y,Tang S,Chen W Z,et al.Research of nitrogen granular fertilizer alleviating high temperature stress at rice grain filling stage and its physiological mechanism(In Chinese).J o u r n a l of Nanjing Agricultural University,2017,40(1):1-10
[16]陈雪,沈方科,梁欢婷,等.外源施硒措施对水稻产量品质及植株硒分布的影响.南方农业学报,2017,48(1):46-50Chen X,Shen F K,Liang H T,et al.Effects of exogenous selenium application on rice yield,quality,distribution of selenium in seedling(In Chinese).Journal of Southern Agriculture,2017,48(1):46-50
[17]Boldrin P F,Faquin V,Ramos S J,et al.Soil and foliar application of selenium in rice biofortification.Journal of Food Composition and Analysis,2013,31:238-244
[18]Barrow N J,Whelan B R.Testing a mechanistic model.VII.The effects of pH and of electrolyte on the reaction of selenite and selenate with a soil.Journal of Soil Science,1989,40(1):17-28
[19]Neal R H,Sposito G.Selenate adsorption on alluvial soils.Soil Science Society of America Journal,1989,53(1):70-74
[20]Rovira M,Gimenez J,Martinez M,et al.Sorption of selenium(IV)and selenium(VI)onto natural iron oxides:goethite and hematite.Journal of Hazardous Materials,2008,150:279-284
[21]Deng X F,Liu K Z,Li M F,et al.Difference of selenium uptake and distribution in the plant and selenium form in the grains of rice with foliar spray of selenite or selenate at different stages.Field Crop Research,2017,211:165-171
[22]Longchamp M,Cas trec-Rouel le M,Bi ron P,et al.Variations in the accumulation,localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenite.Food Chemistry,2015,182:128-135
[23]赵萍,刘笑笑,王雅,等.富硒小麦提取物中硒含量及其抗氧化特性.食品科学,2014(15):94-98Zhao P,Liu X X,Wang Y,et al.Selenium content and antioxidant activity of selenium-enriched wheat(In Chinese).Food Science,2014(15):94-98
[24]Pilon-Smits E A H,Quinn C F.Selenium metabolism in plants.Cell Biology of Metals and Nutrients,2010,17:225-241
[25]Terry N,Zayed A M,de Souza M P,et al.Selenium in higher plants.Annual Review of Plant Physiology&Plant Molecular Biology,2000,51:401-432
[26]Anderson J W.Selenium interact ion s in s u l fur metabolism//Sulfur nutrition and assimilation in higher plants:Regulatory,agricultural and environmental aspects.The Netherlands The Hague:SPB Academic Publishing,1993:49-60
[27]Sort T G,Ellis D R,Salt D E.Selenium uptake,translocation,assimilation and metabolic fate in plants.Photosynthesis Research,2005,86(3):373-389
[28]Zhang L H,Hu B,Li W,et al.OsPT2,a phosphate transporter,is involved in the active uptake of selenite in rice.New Phytologist,2014,201:1183-1191
[29]Das S,Chou M L,Jean J S,et al.Water management impacts on arsenic behavior and rhizosphere bacterial communities and activities in a rice agro-ecosystem.Science of the Total Environment,2015,542(Pt A):642-652
[30]Feng R W,Wei C Y,Tu S X,et al.Effects of Se on the uptake of essential elements in Pteris vittata L..Plant and Soil,2009,325:123-132
[31]Barack P,Goldman I L.Antagonistic relationship between selenate and sulfate uptake in onion(Allium c e p a):I m p l i c a t i o n s f o r t h e p r o d u c t i o n o f organosulfur and organoselenium compounds in plants.Journal of Agricultural and Food Chemistry,1997,45(4):1290-1294
[32]周遗品.硒对水稻蛋白质和氨基酸含量影响的初步研究.石河子农学院学报,1995,31(3):19-22Zhou Y P.The effect of selenium on the contents of proteins and ammonia-acids in rice(In Chinese).Journal of Shihezi Agricultural College,1995,31(3):19-22
[33]Wanger P D.Selenocompounds in plants and animals and their biological signif icance.Journal of the American College of Nutrition,2002,21(3):223-232
[34]Alves M,Chicau P,Matias H,et al.Metabolic analys is revealed al tered amino acid prof i les in Lupinus albus organs as a result of boron deficiency.Physiologia Plantarum,2011,142(3):224-232
[35]朱晔荣,陶汉林,吕宪禹,等.细胞分裂素对光呼吸途径的调节及抗衰老分子机制//中国植物生理学会.中国植物生理学会全国学术年会暨成立40周年庆祝大会学术论文摘要汇编.杭州:中国植物生理学会,2003:199Zhu Y R,Tao H L,LüX Y,et al.Cytokinin on the photorespiratory pathway regulation and molecular mechanism of anti-aging//The Chinese Society for Plant Physiology.The collection of abstracts of Thesis on the National Academic Annual Conference and40th Anniversary Celebration of The Chinese Society for Plant Physiology(In Chinese).Hangzhou:The Chinese Society for Plant Physiology,2003:199
[2]Xia Y M,Hill K E,Byrne D W,et al.Effectiveness of selenium supplements in a low-selenium area of China.The American Journal of Clinical Nutrition,2005,81:829-834
[3]Sharma P,Aggarwal P,Kaur A.Biofortification:A new approach to eradicate hidden hunger.Food Reviews International,2017,33(1):1-21
[4]周鑫斌,施卫明,杨林章.富硒与非富硒水稻品种对硒的吸收分配的差异及机理.土壤,2007,39(5):731-736Zhou X B,Shi W M,Yang L Z.Genotypical differences and characteristics of Se uptake and accumulation in rice(In Chinese).Soils,2007,39(5):731-736
[5]周鑫斌,施卫明,杨林章.叶面喷硒对水稻籽粒硒富集及分布的影响.土壤学报,2007,44(1):73-78Zhou X B,Shi W M,Yang L Z.Effect of foliar application of selenite on selenium accumulation and distribution in rice(In Chinese).Acta Pedologica Sinica,2007,44(1):73-78
[6]Sun G X,Liu X,Williams P N,et al.Distribution and translocation of selenium from soil to grain and its speciation in paddy rice(Oryza sativa L.).Environmental Science and Technology,2010,44(17):6706-6711
[7]Carey A M,Scheckel K G,Lombi E,et al.Grain accumulation of selenium species in rice(Oryza sativa L.).Environmental Science and Technology,2012,46(10):5557-5564
[8]Li H F,Mcgrath S P,Zhao F J.Selenium uptake,translocation and speciation in wheat supplied with selenate or selenite.New Phytologist,2008,178(1):92-102
[9]El Kassis E,Cathala N,Rouached H,et al.Characterization of a selenate-resistant Arabidopsis mutant.Root growth as a potential target for selenate toxicity.Plant Physiology,2007,143(3):1231-1241
[10]Fujii R,Deverel S J,Hatfield D B.Distribution of selenium in soils of agricultural fields,western San Joaquin Valley,California.Soil Science Society of America Journal,1988,52(5):1274-1283
[11]杨剑虹,王成林,代亨林.土壤农化分析与环境监测.北京:中国大地出版社,2008Yang J H,Wang C L,Dai H L.Soil chemical analysis and environmental monitoring(In Chinese).Beijing:China Land Press,2008
[12]Zhang L H,Shi W M,Wang X C.Difference in selenite absorption between high-and low-selenium rice cultivars and its mechanism.Plant and Soil,2006,282(1):183-193
[13]Amagliani L,O’Regan J,Keny A L,et al.Composition and protein profile analysis of rice protein ingredients.Journal of Food Composition and Analysis,2017,59:18-26
[14]中华人民共和国农业部.NY/T83-1988:米质测定方法.北京:中国标准出版社,1988The Ministry of Agriculture of the People’s Republic of China.NY/T83-1988:The standard for the method of the rice quality determination(In Chinese).Beijing:China Standard Press,1988
[15]穆乃耀,唐设,陈文珠,等.氮素粒肥缓解水稻灌浆期高温胁迫的生理机制研究.南京农业大学学报,2017,40(1):1-10Mu N Y,Tang S,Chen W Z,et al.Research of nitrogen granular fertilizer alleviating high temperature stress at rice grain filling stage and its physiological mechanism(In Chinese).J o u r n a l of Nanjing Agricultural University,2017,40(1):1-10
[16]陈雪,沈方科,梁欢婷,等.外源施硒措施对水稻产量品质及植株硒分布的影响.南方农业学报,2017,48(1):46-50Chen X,Shen F K,Liang H T,et al.Effects of exogenous selenium application on rice yield,quality,distribution of selenium in seedling(In Chinese).Journal of Southern Agriculture,2017,48(1):46-50
[17]Boldrin P F,Faquin V,Ramos S J,et al.Soil and foliar application of selenium in rice biofortification.Journal of Food Composition and Analysis,2013,31:238-244
[18]Barrow N J,Whelan B R.Testing a mechanistic model.VII.The effects of pH and of electrolyte on the reaction of selenite and selenate with a soil.Journal of Soil Science,1989,40(1):17-28
[19]Neal R H,Sposito G.Selenate adsorption on alluvial soils.Soil Science Society of America Journal,1989,53(1):70-74
[20]Rovira M,Gimenez J,Martinez M,et al.Sorption of selenium(IV)and selenium(VI)onto natural iron oxides:goethite and hematite.Journal of Hazardous Materials,2008,150:279-284
[21]Deng X F,Liu K Z,Li M F,et al.Difference of selenium uptake and distribution in the plant and selenium form in the grains of rice with foliar spray of selenite or selenate at different stages.Field Crop Research,2017,211:165-171
[22]Longchamp M,Cas trec-Rouel le M,Bi ron P,et al.Variations in the accumulation,localization and rate of metabolization of selenium in mature Zea mays plants supplied with selenite or selenite.Food Chemistry,2015,182:128-135
[23]赵萍,刘笑笑,王雅,等.富硒小麦提取物中硒含量及其抗氧化特性.食品科学,2014(15):94-98Zhao P,Liu X X,Wang Y,et al.Selenium content and antioxidant activity of selenium-enriched wheat(In Chinese).Food Science,2014(15):94-98
[24]Pilon-Smits E A H,Quinn C F.Selenium metabolism in plants.Cell Biology of Metals and Nutrients,2010,17:225-241
[25]Terry N,Zayed A M,de Souza M P,et al.Selenium in higher plants.Annual Review of Plant Physiology&Plant Molecular Biology,2000,51:401-432
[26]Anderson J W.Selenium interact ion s in s u l fur metabolism//Sulfur nutrition and assimilation in higher plants:Regulatory,agricultural and environmental aspects.The Netherlands The Hague:SPB Academic Publishing,1993:49-60
[27]Sort T G,Ellis D R,Salt D E.Selenium uptake,translocation,assimilation and metabolic fate in plants.Photosynthesis Research,2005,86(3):373-389
[28]Zhang L H,Hu B,Li W,et al.OsPT2,a phosphate transporter,is involved in the active uptake of selenite in rice.New Phytologist,2014,201:1183-1191
[29]Das S,Chou M L,Jean J S,et al.Water management impacts on arsenic behavior and rhizosphere bacterial communities and activities in a rice agro-ecosystem.Science of the Total Environment,2015,542(Pt A):642-652
[30]Feng R W,Wei C Y,Tu S X,et al.Effects of Se on the uptake of essential elements in Pteris vittata L..Plant and Soil,2009,325:123-132
[31]Barack P,Goldman I L.Antagonistic relationship between selenate and sulfate uptake in onion(Allium c e p a):I m p l i c a t i o n s f o r t h e p r o d u c t i o n o f organosulfur and organoselenium compounds in plants.Journal of Agricultural and Food Chemistry,1997,45(4):1290-1294
[32]周遗品.硒对水稻蛋白质和氨基酸含量影响的初步研究.石河子农学院学报,1995,31(3):19-22Zhou Y P.The effect of selenium on the contents of proteins and ammonia-acids in rice(In Chinese).Journal of Shihezi Agricultural College,1995,31(3):19-22
[33]Wanger P D.Selenocompounds in plants and animals and their biological signif icance.Journal of the American College of Nutrition,2002,21(3):223-232
[34]Alves M,Chicau P,Matias H,et al.Metabolic analys is revealed al tered amino acid prof i les in Lupinus albus organs as a result of boron deficiency.Physiologia Plantarum,2011,142(3):224-232
[35]朱晔荣,陶汉林,吕宪禹,等.细胞分裂素对光呼吸途径的调节及抗衰老分子机制//中国植物生理学会.中国植物生理学会全国学术年会暨成立40周年庆祝大会学术论文摘要汇编.杭州:中国植物生理学会,2003:199Zhu Y R,Tao H L,LüX Y,et al.Cytokinin on the photorespiratory pathway regulation and molecular mechanism of anti-aging//The Chinese Society for Plant Physiology.The collection of abstracts of Thesis on the National Academic Annual Conference and40th Anniversary Celebration of The Chinese Society for Plant Physiology(In Chinese).Hangzhou:The Chinese Society for Plant Physiology,2003:199