划胚方法对玉米单倍体加倍效率的影响
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摘要
随着新型诱导系在育种领域的选育和诱导效率的提高,玉米单倍体加倍技术发展也至关重要,但现有单倍体加倍方法效率普遍较低。为了进一步提高玉米单倍体的加倍效率,探索最佳的加倍条件,采用对玉米籽粒胚部划胚技术及不同质量分数的秋水仙素和不同处理时间对玉米单倍体籽粒进行划胚加倍。结果表明,对玉米单倍体的划胚处理显著高于自然加倍;不同质量分数的秋水仙素及不同处理时间对加倍后植株散粉率等影响显著,其中,秋水仙素质量分数为0.04%、处理时间3 h的散粉率最高,加倍效果最佳。
With the breeding and induction efficiency of new inducing lines in the field of breeding, the development of maize haploid doubling technology is also crucial, but the efficiency of existing haploid doubling methods is generally lower. To further improve the doubling efficiency of maize haploids, explore the best doubling conditions, the paper used the technique of embryo sizing of corn kernels and double the ploidy of maize haploid seeds by using different mass fraction of colchicine and different treatment time. The results showed that the treatment of maize haploid was significantly higher than natural doubling, the colchicine with different mass fractions and different treatment time had significant effects on the powder loosening rate after doubling, when the mass fraction of colchicine was 0.04%, and the treatment time was 3 h, the powder dispersal rale was the highest and the doubling effect was the best.
With the breeding and induction efficiency of new inducing lines in the field of breeding, the development of maize haploid doubling technology is also crucial, but the efficiency of existing haploid doubling methods is generally lower. To further improve the doubling efficiency of maize haploids, explore the best doubling conditions, the paper used the technique of embryo sizing of corn kernels and double the ploidy of maize haploid seeds by using different mass fraction of colchicine and different treatment time. The results showed that the treatment of maize haploid was significantly higher than natural doubling, the colchicine with different mass fractions and different treatment time had significant effects on the powder loosening rate after doubling, when the mass fraction of colchicine was 0.04%, and the treatment time was 3 h, the powder dispersal rale was the highest and the doubling effect was the best.
引文
[1]崔学宇.玉米单倍体诱导系对DH系影响的研究及DH系遗传分析[D].长春:吉林农业大学,2016.
[2]杨庆才.关于玉米产业经济发展战略的思考[J].农业经济问题,2008,18(7):4-9.
[3]刘纪麟.玉米育种学[M]. 2版.北京:中国农业出版社,2002.
[4]陈绍江,黎亮,李浩川.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009.
[5]周联东,孙佩,王文洁,等.玉米单倍体诱导系EMK-1诱导率的测定和单倍体自然加倍技术研究[J].河南农业科学,2015,44(9):19-21.
[6]段民孝,刘新香,赵久然,等.秋水仙素浸芽法处理玉米单倍体的加倍效果研究[J].种子,2013,32(2):19-23.
[7]魏俊杰,张晓丽,陈梅香,等. 6叶期秋水仙素注射处理玉米单倍体的加倍效果研究[J].玉米科学,2007,15(4):49-51.
[8]GAYEN P,SARKAR K R. Cytomixis in maize haploids[J]. Indian J Genet Pla nt Breeding,1996,56:79-85.
[9]文科,黎亮,刘玉强,等.高效生物诱导玉米单倍体及其加倍方法研究初报[J].中国农业大学学报,2006,11(5):17-20.
[10]刘海,郭建文,郑娟,等.单倍体玉米不同方式加倍后的散粉率与结实率[J].山西农业科学,2015,43(7):780-782.
[11] CHASE S S. Production of homozygous diploids of maize from monoploids 1[J]. Agronomy Journal,1952,44(5):263-267.
[12]陈宝健,刘丽威,徐丽,等.玉米单倍体幼胚加倍效果观察[J].中国农业大学学报,2016,21(5):10-16.
[13]SEANEY R R. Studies on nomoploidy in maize[D]. New York:Cornell Univ.,Ithaca,1955.
[14] GAYEN P,JASBIR K M,RAJESH K,et al. Chromosome doubling in haploids through colchicines[J]. Maize Genet Coop Newsl,1994,68:64-65.
[15]刘志增,宋同明.玉米单倍体雌雄育性的自然恢复以及染色体的化学加倍[J].作物学报,2000,26(6):947-952.
[16]刘俊,尹晓红,刘君,等.不同加倍方法处理玉米单倍体的加倍效果研究[C]//第一届全国玉米生物学学术研讨会论文汇编.北京:中国作物学会,2015.
[17]惠国强,杜何为,杨小红,等.不同除草剂加倍玉米单倍体的效率[J].作物学报,2012,38(3):416-422.
[18]范志青.玉米单倍体加倍技术研究[D].长春:中国科学院东北地理与农业生态研究所,2016.
[19]谢琦.基于幼胚成苗技术的玉米加倍方法研究[D].长春:中国科学院东北地理与农业生态研究所,2015.
[20]李浩川,李颜,曲彦志,等.玉米单倍体成熟胚组培加倍效果研究[J].河南农业大学学报,2018,52(1):1-5.
[21]杜文平,余桂容,陈谦,等.切根处理对玉米单倍体加倍效率的影响[J].玉米科学,2017,25(4):12-16.
[22]段民孝,刘新香,赵久然,等.秋水仙素浸芽法处理玉米单倍体的加倍效果研究[J].种子,2013,32(2):19-23.
[2]杨庆才.关于玉米产业经济发展战略的思考[J].农业经济问题,2008,18(7):4-9.
[3]刘纪麟.玉米育种学[M]. 2版.北京:中国农业出版社,2002.
[4]陈绍江,黎亮,李浩川.玉米单倍体育种技术[M].北京:中国农业大学出版社,2009.
[5]周联东,孙佩,王文洁,等.玉米单倍体诱导系EMK-1诱导率的测定和单倍体自然加倍技术研究[J].河南农业科学,2015,44(9):19-21.
[6]段民孝,刘新香,赵久然,等.秋水仙素浸芽法处理玉米单倍体的加倍效果研究[J].种子,2013,32(2):19-23.
[7]魏俊杰,张晓丽,陈梅香,等. 6叶期秋水仙素注射处理玉米单倍体的加倍效果研究[J].玉米科学,2007,15(4):49-51.
[8]GAYEN P,SARKAR K R. Cytomixis in maize haploids[J]. Indian J Genet Pla nt Breeding,1996,56:79-85.
[9]文科,黎亮,刘玉强,等.高效生物诱导玉米单倍体及其加倍方法研究初报[J].中国农业大学学报,2006,11(5):17-20.
[10]刘海,郭建文,郑娟,等.单倍体玉米不同方式加倍后的散粉率与结实率[J].山西农业科学,2015,43(7):780-782.
[11] CHASE S S. Production of homozygous diploids of maize from monoploids 1[J]. Agronomy Journal,1952,44(5):263-267.
[12]陈宝健,刘丽威,徐丽,等.玉米单倍体幼胚加倍效果观察[J].中国农业大学学报,2016,21(5):10-16.
[13]SEANEY R R. Studies on nomoploidy in maize[D]. New York:Cornell Univ.,Ithaca,1955.
[14] GAYEN P,JASBIR K M,RAJESH K,et al. Chromosome doubling in haploids through colchicines[J]. Maize Genet Coop Newsl,1994,68:64-65.
[15]刘志增,宋同明.玉米单倍体雌雄育性的自然恢复以及染色体的化学加倍[J].作物学报,2000,26(6):947-952.
[16]刘俊,尹晓红,刘君,等.不同加倍方法处理玉米单倍体的加倍效果研究[C]//第一届全国玉米生物学学术研讨会论文汇编.北京:中国作物学会,2015.
[17]惠国强,杜何为,杨小红,等.不同除草剂加倍玉米单倍体的效率[J].作物学报,2012,38(3):416-422.
[18]范志青.玉米单倍体加倍技术研究[D].长春:中国科学院东北地理与农业生态研究所,2016.
[19]谢琦.基于幼胚成苗技术的玉米加倍方法研究[D].长春:中国科学院东北地理与农业生态研究所,2015.
[20]李浩川,李颜,曲彦志,等.玉米单倍体成熟胚组培加倍效果研究[J].河南农业大学学报,2018,52(1):1-5.
[21]杜文平,余桂容,陈谦,等.切根处理对玉米单倍体加倍效率的影响[J].玉米科学,2017,25(4):12-16.
[22]段民孝,刘新香,赵久然,等.秋水仙素浸芽法处理玉米单倍体的加倍效果研究[J].种子,2013,32(2):19-23.