内生枯草芽孢杆菌265ZY4对温度和紫外光胁迫下紫花针茅生化特征的影响
|
摘要
在高、低温和紫外线胁迫下,将高寒草地紫花针茅的内生细菌265ZY4回接至紫花针茅测定其对寄主抗胁迫能力的影响。结果表明,接种265ZY4后紫花针茅的株高增加35.02%,叶宽增加34.69%;在低温、高温和紫外线胁迫下带菌紫花针茅叶绿素含量高于对照,温度胁迫下叶绿素呈先上升后下降,但紫外线胁迫下呈快速下降;在低温和紫外线胁迫下带菌紫花针茅可溶性糖含量分别在第4和6天后显著高于对照,但可溶性蛋白含量低于对照,温度和紫外线胁迫下可溶性蛋白和可溶性糖呈"上升-下降"的趋势;低温胁迫下带菌紫花针茅超氧化物歧化酶(SOD)活性变化显著小于对照,带菌及对照均呈"降低-升高-降低"的规律;紫外线胁迫下带菌植株SOD活性呈现"下降-上升"的趋势,第8天时显著高于对照,对照表现为"下降-上升-下降"的趋势;在低温和紫外线胁迫下带菌紫花针茅苯丙氨酸解氨酶(PAL)活性高于对照,且在低温胁迫下呈"上升-下降"的趋势,但在高温和紫外线胁迫下呈"下降-上升-下降"的趋势。试验结果表明,内生细菌265ZY4有助于维持逆境胁迫下宿主生化指标的稳定,提高宿主对逆境的忍耐能力。
In order to understand the effects of the endophytic bacterium, Bacillus subtilis, on Stipa purpurea under high temperature, low temperature and ultraviolet stress, the biochemical characteristics of S. purpurea inoculated with B. subtilis strain 265 ZY4 were compared with those of plants not carrying the bacterial endophyte. The results showed that the height and leaf width of S. purpurea with 265 ZY4 were 35.02% and 34.69%, respectively, more than in the control plants. When S. purpurea was subject to high-or low-temperature stress, leaf chlorophyll content rose and then fell. Under ultraviolet stress, chlorophyll levels dropped rapidly, but the chlorophyll content of S. purpurea with 265 ZY4 remained higher than those in the control. The soluble protein and soluble sugar levels also initially rose and then dropped under abiotic stress, but the soluble sugar levels of S. purpurea with 265 ZY4 were distinctly higher than those in the control plants after 4 and 6 days. The superoxide dismutase(SOD) activity displayed a decrease-increase-decrease curve with progressive increase in high-and low-temperature stress, but the SOD activity of S. purpurea with 265 ZY4 was more stable. The SOD activity of the control plants showed a decrease-increase-decrease curve pattern under increasing ultraviolet exposure, but the activity of S. purpurea with 265 ZY4 showed a curve pattern that differed from the control plants. The phenylalnine ammonialyase(PAL) activity displayed an increase-decrease curve pattern under low temperature stress and a decrease-increase-decrease curve under high temperature and ultraviolet stress, but the PAL activities of S. purpurea plants with 265 ZY4 were distinctly higher than those in the control. In summary, the results show that the endophytic B. subtilis strain 265 ZY4 is beneficial to S. pupurea through assisting with maintenance of chlorophyll, soluble protein, and soluble sugar levels, and SOD and PAL activities, and reducing the range of metabolic variability under the abiotic stress factors tested, thus indicating enhancement of S. purpurea stress tolerance ability.
In order to understand the effects of the endophytic bacterium, Bacillus subtilis, on Stipa purpurea under high temperature, low temperature and ultraviolet stress, the biochemical characteristics of S. purpurea inoculated with B. subtilis strain 265 ZY4 were compared with those of plants not carrying the bacterial endophyte. The results showed that the height and leaf width of S. purpurea with 265 ZY4 were 35.02% and 34.69%, respectively, more than in the control plants. When S. purpurea was subject to high-or low-temperature stress, leaf chlorophyll content rose and then fell. Under ultraviolet stress, chlorophyll levels dropped rapidly, but the chlorophyll content of S. purpurea with 265 ZY4 remained higher than those in the control. The soluble protein and soluble sugar levels also initially rose and then dropped under abiotic stress, but the soluble sugar levels of S. purpurea with 265 ZY4 were distinctly higher than those in the control plants after 4 and 6 days. The superoxide dismutase(SOD) activity displayed a decrease-increase-decrease curve with progressive increase in high-and low-temperature stress, but the SOD activity of S. purpurea with 265 ZY4 was more stable. The SOD activity of the control plants showed a decrease-increase-decrease curve pattern under increasing ultraviolet exposure, but the activity of S. purpurea with 265 ZY4 showed a curve pattern that differed from the control plants. The phenylalnine ammonialyase(PAL) activity displayed an increase-decrease curve pattern under low temperature stress and a decrease-increase-decrease curve under high temperature and ultraviolet stress, but the PAL activities of S. purpurea plants with 265 ZY4 were distinctly higher than those in the control. In summary, the results show that the endophytic B. subtilis strain 265 ZY4 is beneficial to S. pupurea through assisting with maintenance of chlorophyll, soluble protein, and soluble sugar levels, and SOD and PAL activities, and reducing the range of metabolic variability under the abiotic stress factors tested, thus indicating enhancement of S. purpurea stress tolerance ability.
引文
[1]Wang Z W,Chen Y G,Wang Q C,et al.Progresses and perspectives of studies on plant endophytic microbes in China.Microbiology China,2014,41(3):482-496.王志伟,陈永敢,王庆璨,等.中国植物内生微生物研究的发展和展望.微生物学通报,2014,41(3):482-496.
[2]Qin J J,Yan S Z,Liu J.The growth-promotion on pepper and control of phytophthora capsici by endophytic bacterium agents.Acta Phytophylacica Sinica,2010,4:325-330.秦娟娟,闫淑珍,刘佳.植物内生细菌固体菌剂对辣椒的促生和防病作用.植物保护学报,2010,4:325-330.
[3]Zhang F F,Song X Q,Ding Q,et al.Activity analysis of growth-promoting endophytic bacteria isolated from the roots of Phalaenopsis pulcherrima.Journal of Tropical Biology,2015,6(3):285-292.张芳芳,宋希强,丁琼,等.五唇兰根部内生细菌的筛选及其促生活性.热带生物学报,2015,6(3):285-292.
[4]Bin Y,Asami S,Kiwamu M.Effect of inoculation with anaerobic nitrogen-fixing consortium on salt tolerance of Miscanthus sinensis.Soil Science and Plant Nutrition,2005,52(2):243-249.
[5]Luo D,Pan C D,Zhou J,et al.Effect of inoculation with endophytic diazotrophic bacteria on physiological characters of Tamarix ramosissima Ledeb.seedlings under salt-drought stress.Arid Land Geography,2012,35(1):154-161.罗达,潘存德,周俊,等.干旱及盐双胁迫下内生固氮菌接种对多枝柽柳实生苗生理特性的影响.干旱区地理,2012,35(1):154-161.
[6]Yue P P,Sun J,Lu X F,et al.Quantitative classification and ordination of Stipapurpereasteppe community in Qinghai Plateau.Acta Botanica Boreali-Occidentalia Sinica,2014,34(5):1047-1054.岳鹏鹏,孙菁,卢学峰,等.青海高原紫花针茅草原群落数量分类与排序.西北植物学报,2014,34(5):1047-1054.
[7]Wang Y Q,Yang C D,Wang Y,et al.Identification and determination of biological functions of endophytic bacteria 265ZY4fromStipa capillata.Microbiology China,2015,42(1):101-109.王玉琴,杨成德,王颖,等.针茅内生细菌菌株265ZY4的鉴定及其生物学功能.微生物学通报,2015,42(1):101-109.
[8]Li H S.Principles and techniques of plant physiology and biochemistry experiments.Beijing:Higher Education Press,2000.李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000.
[9]Zhang Z L,Qu W J,Li X F.Experimental guidance of plant physiology.BeiJing:Higher Education Press,2009.张志良,瞿伟菁,李小方.植物生理学实验指导.北京:高等教育出版社,2009.
[10]Gao J F.Experimental guidance of plant physiology.Beijing:Higher Education Press,2006.高峻凤.植物生理学实验指导.北京:高等教育出版社,2006.
[11]Guo B,Wang Y,Sun X,et al.Bioactive natural products from endophytes:A Review.Applied Biochemistry and Microbiology,2008,44(2):136-142.
[12]Narendra B A,Jogaiah S,Ito S,et al.Improvement of growth,fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria is correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase.Plant Science,2015,231:62-73.
[13]Wang Z,Liu X X,Zheng G H.Effects of low temperature stress on the photosynthesis and chlorophyll fluorescence of Solenostemon scutellarioides.Acta Agriculturae Zhejiangensis,2015,27(1):49-56.王兆,刘晓曦,郑国华.低温胁迫对彩叶草光合作用及叶绿素荧光的影响.浙江农业学报,2015,27(1):49-56.
[14]Sohrabi Y,Heidari G.Changes of antioxidative enzymes,lipid peroxidation and chlorophyll content in chickpea types colonized by different glomus species under drought stress.Symbiosis,2012,56(1):5-18.
[15]Wu X C,Lin W X,Huang Z L.Influence of enhanced ultraviolet-B radiation on photosynthetic physiologies and ultrastructure of leaves in two different resistivity rice cultivars.Acta Ecologica Sinica,2007,27(2):554-564.吴杏春,林文雄,黄忠良.UV-B辐射增强对两种不同抗性水稻叶片光合生理及超显微结构的影响.生态学报,2007,27(2):554-564.
[16]Moieni K Z,Karimzadeh G,Sharifi M.Evaluation of total solubleprotein and antioxidant activities in two spring cultivars of canola(Brassica napus L.)in response to low temperature.International Journal of Agriculture and Crop Sciences,2013,5(4):401-409.
[17]Sun F,Yang L T,Xie X N,et al.Effect of chilling stress on physiological metabolism in chloroplasts of seedlings of sugarcane varieties with different chilling resistance.Acta Agronomica Sinica,2012,38(4):732-739.孙富,杨丽涛,谢晓娜,等.低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响.作物学报,2012,38(4):732-739.
[18]Yang S,Guo H P,Hao G W,et al.The study of the cold resistance and physiology index of some pear varieties in Shanxi Province.Journal of Agriculture,2014,4(12):72-77.杨盛,郭黄萍,郝国伟,等.山西部分梨品种抗寒性测定及生理指标研究.农学学报,2014,4(12):72-77.
[19]Yang Y Z,Peng F R.The effect of low-temperature stress upon protein content and component in leaves of Toona sinensis seedlings.Chinese Agricultural Science Bulletin,2010,26(7):115-119.杨玉珍,彭方仁.低温胁迫对香椿幼苗蛋白质含量及特异蛋白表达的影响.中国农学通报,2010,26(7):115-119.
[20]Qian Y Q,Sun Z Y,Han L,et al.Response of reactive oxygen and its scavenging system in leaves of Buchloe dactyloides(Nutt.)engelm to water stress.Acta Ecologica Sinica,2010,30(7):1920-1926钱永强,孙振元,韩蕾,等.野牛草叶片活性氧及其清除系统对水分胁迫的响应.生态学报,2010,30(7):1920-1926.
[21]Zhou R L,Zhao H L.Seasonal pattern of antioxidant enzyme system in the roots of perennial forage grasses grown in alpine habitat,related to freezing tolerance.Physiologia Plantarum,2004,121(3):399-408.
[22]Chen L,Chang L,Cao F L,et al.Effects of temperature and soil water deficit on the flavonoid content and activities of enzymes involved in ginkgo leaves.Acta Botanica Boreali-Occidentalia Sinica,2013,33(4):755-762.陈雷,常丽,曹福亮,等.银杏叶黄酮类化合物含量及相关酶活性对温度和干旱胁迫的响应.西北植物学报,2013,33(4):755-762.
[23]Sun K,Li S Y.Feasibility analysis for mitigating the contamination of POPs in crops through inoculation with functional endophytic bacteria.Journal of Agricultural Resources and Environment,2017,34(5):397-404.孙凯,李舜尧.接种功能内生细菌降低作物体内POPs污染.农业资源与环境学报,2017,34(5):397-404.
[2]Qin J J,Yan S Z,Liu J.The growth-promotion on pepper and control of phytophthora capsici by endophytic bacterium agents.Acta Phytophylacica Sinica,2010,4:325-330.秦娟娟,闫淑珍,刘佳.植物内生细菌固体菌剂对辣椒的促生和防病作用.植物保护学报,2010,4:325-330.
[3]Zhang F F,Song X Q,Ding Q,et al.Activity analysis of growth-promoting endophytic bacteria isolated from the roots of Phalaenopsis pulcherrima.Journal of Tropical Biology,2015,6(3):285-292.张芳芳,宋希强,丁琼,等.五唇兰根部内生细菌的筛选及其促生活性.热带生物学报,2015,6(3):285-292.
[4]Bin Y,Asami S,Kiwamu M.Effect of inoculation with anaerobic nitrogen-fixing consortium on salt tolerance of Miscanthus sinensis.Soil Science and Plant Nutrition,2005,52(2):243-249.
[5]Luo D,Pan C D,Zhou J,et al.Effect of inoculation with endophytic diazotrophic bacteria on physiological characters of Tamarix ramosissima Ledeb.seedlings under salt-drought stress.Arid Land Geography,2012,35(1):154-161.罗达,潘存德,周俊,等.干旱及盐双胁迫下内生固氮菌接种对多枝柽柳实生苗生理特性的影响.干旱区地理,2012,35(1):154-161.
[6]Yue P P,Sun J,Lu X F,et al.Quantitative classification and ordination of Stipapurpereasteppe community in Qinghai Plateau.Acta Botanica Boreali-Occidentalia Sinica,2014,34(5):1047-1054.岳鹏鹏,孙菁,卢学峰,等.青海高原紫花针茅草原群落数量分类与排序.西北植物学报,2014,34(5):1047-1054.
[7]Wang Y Q,Yang C D,Wang Y,et al.Identification and determination of biological functions of endophytic bacteria 265ZY4fromStipa capillata.Microbiology China,2015,42(1):101-109.王玉琴,杨成德,王颖,等.针茅内生细菌菌株265ZY4的鉴定及其生物学功能.微生物学通报,2015,42(1):101-109.
[8]Li H S.Principles and techniques of plant physiology and biochemistry experiments.Beijing:Higher Education Press,2000.李合生.植物生理生化实验原理和技术.北京:高等教育出版社,2000.
[9]Zhang Z L,Qu W J,Li X F.Experimental guidance of plant physiology.BeiJing:Higher Education Press,2009.张志良,瞿伟菁,李小方.植物生理学实验指导.北京:高等教育出版社,2009.
[10]Gao J F.Experimental guidance of plant physiology.Beijing:Higher Education Press,2006.高峻凤.植物生理学实验指导.北京:高等教育出版社,2006.
[11]Guo B,Wang Y,Sun X,et al.Bioactive natural products from endophytes:A Review.Applied Biochemistry and Microbiology,2008,44(2):136-142.
[12]Narendra B A,Jogaiah S,Ito S,et al.Improvement of growth,fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria is correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase.Plant Science,2015,231:62-73.
[13]Wang Z,Liu X X,Zheng G H.Effects of low temperature stress on the photosynthesis and chlorophyll fluorescence of Solenostemon scutellarioides.Acta Agriculturae Zhejiangensis,2015,27(1):49-56.王兆,刘晓曦,郑国华.低温胁迫对彩叶草光合作用及叶绿素荧光的影响.浙江农业学报,2015,27(1):49-56.
[14]Sohrabi Y,Heidari G.Changes of antioxidative enzymes,lipid peroxidation and chlorophyll content in chickpea types colonized by different glomus species under drought stress.Symbiosis,2012,56(1):5-18.
[15]Wu X C,Lin W X,Huang Z L.Influence of enhanced ultraviolet-B radiation on photosynthetic physiologies and ultrastructure of leaves in two different resistivity rice cultivars.Acta Ecologica Sinica,2007,27(2):554-564.吴杏春,林文雄,黄忠良.UV-B辐射增强对两种不同抗性水稻叶片光合生理及超显微结构的影响.生态学报,2007,27(2):554-564.
[16]Moieni K Z,Karimzadeh G,Sharifi M.Evaluation of total solubleprotein and antioxidant activities in two spring cultivars of canola(Brassica napus L.)in response to low temperature.International Journal of Agriculture and Crop Sciences,2013,5(4):401-409.
[17]Sun F,Yang L T,Xie X N,et al.Effect of chilling stress on physiological metabolism in chloroplasts of seedlings of sugarcane varieties with different chilling resistance.Acta Agronomica Sinica,2012,38(4):732-739.孙富,杨丽涛,谢晓娜,等.低温胁迫对不同抗寒性甘蔗品种幼苗叶绿体生理代谢的影响.作物学报,2012,38(4):732-739.
[18]Yang S,Guo H P,Hao G W,et al.The study of the cold resistance and physiology index of some pear varieties in Shanxi Province.Journal of Agriculture,2014,4(12):72-77.杨盛,郭黄萍,郝国伟,等.山西部分梨品种抗寒性测定及生理指标研究.农学学报,2014,4(12):72-77.
[19]Yang Y Z,Peng F R.The effect of low-temperature stress upon protein content and component in leaves of Toona sinensis seedlings.Chinese Agricultural Science Bulletin,2010,26(7):115-119.杨玉珍,彭方仁.低温胁迫对香椿幼苗蛋白质含量及特异蛋白表达的影响.中国农学通报,2010,26(7):115-119.
[20]Qian Y Q,Sun Z Y,Han L,et al.Response of reactive oxygen and its scavenging system in leaves of Buchloe dactyloides(Nutt.)engelm to water stress.Acta Ecologica Sinica,2010,30(7):1920-1926钱永强,孙振元,韩蕾,等.野牛草叶片活性氧及其清除系统对水分胁迫的响应.生态学报,2010,30(7):1920-1926.
[21]Zhou R L,Zhao H L.Seasonal pattern of antioxidant enzyme system in the roots of perennial forage grasses grown in alpine habitat,related to freezing tolerance.Physiologia Plantarum,2004,121(3):399-408.
[22]Chen L,Chang L,Cao F L,et al.Effects of temperature and soil water deficit on the flavonoid content and activities of enzymes involved in ginkgo leaves.Acta Botanica Boreali-Occidentalia Sinica,2013,33(4):755-762.陈雷,常丽,曹福亮,等.银杏叶黄酮类化合物含量及相关酶活性对温度和干旱胁迫的响应.西北植物学报,2013,33(4):755-762.
[23]Sun K,Li S Y.Feasibility analysis for mitigating the contamination of POPs in crops through inoculation with functional endophytic bacteria.Journal of Agricultural Resources and Environment,2017,34(5):397-404.孙凯,李舜尧.接种功能内生细菌降低作物体内POPs污染.农业资源与环境学报,2017,34(5):397-404.