广西鸡源H9N2亚型禽流感病毒分离鉴定及遗传进化分析
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摘要
【目的】明确广西鸡源H9N2亚型禽流感病毒(AIV)的分子遗传变异规律及其对公共卫生安全的影响,为H9亚型禽流感(AI)的防控提供参考依据。【方法】从广西某肉鸡养殖场采集病料,经SPF鸡胚接种进行病毒分离,对初步鉴定为H9亚型AIV分离株的HA、NA、PB2、PB1、PA、NP、M和NS等8个基因片段进行克隆测定及遗传进化分析,同时对分离毒株的生物学特性进行测定。【结果】从广西发病鸡群中分离获得一株H9N2亚型AIV,命名为A/chicken/Guangxi/BT1/2016(H9N2)。分离毒株A/chicken/Guangxi/BT1/2016(H9N2)HA基因的裂解位点为PSRSSR↓GLF,具有典型的低致病性AIV(LPAIV)分子特征,在遗传进化关系上属于4.2.5分支,与国内常用H9N2亚型AIV疫苗株所属的4.2.3分支存在一定差异;其他7个基因也分别来源于不同的AIV亚型。分离毒株A/chicken/Guangxi/BT1/2016(H9N2)同时具备与α-2,3唾液酸和α-2,6唾液酸受体结合的特性,且具有感染人类的潜在风险;其感染鸡群后病毒分布主要集中在气管、肺脏和脾脏,可通过呼吸道和消化道同时排毒,且在攻毒后第1 d即开始排毒,第5 d为排毒高峰。【结论】从广西发病鸡群中分离获得的A/chicken/Guangxi/BT1/2016(H9N2)属于LPAIV,是由不同亚型AIV重组产生的H9N2亚型新毒株,可导致鸡群疫苗免疫失败,且具有感染人类的潜在风险。
【Objective】This study determined molecular genetic variation regularity of H9N2 subtype avian influenza virus(AIV)and its effects on public health safety of human so as to provide reference for the prevention and control of H9 subtype avian influenza(AI).【Method】Suspected tissue samples were collected from one chicken farm in Guangxi,viral isolation was performed by inoculating SPF chicken embryo. The virus was preliminarily identified as H9 subtype AIV isolated strain. Then eight gene fragments isolated from H9 subtype AIV including HA,NA,PB2,PB,PA,NP,M and NS were sequenced,and their genetic evolution was analyzed. The biological characteristics of the isolated strain were also determined at the same time.【Result】A H9N2 subtype AIV strain,named A/chicken/Guangxi/BT1/2016(H9N2),was successfully separated from the sick chicken population in Guangxi. The cleavage site of HA gene in A/chicken/Guangxi/BT1/2016(H9N2)was PSRSSR↓GLF,which had typical features of low pathogenic AIV(LPAIV)molecule. In genetic evolution relation,it belonged to 4.2.5 branch,and had some differences with the domestic H9N2 subtype AIV vaccine strains which belonged to 4.2.3 branch. The other seven genes were also derived from different AIV subtypes. The isolated virus strain A/chicken/Guangxi/BT1/2016(H9N2)could bind to α-2,3 sialic acid and α-2,6 sialic acid receptors,with the potential risk to infect human. Virus distribution was mainly concentrated in trachea,lung and spleen after it infected chicken,and the virus also could be released through the respiratory tract and digestive tract at same time. The virus was released from day 1 and peaked on the day 5 after the chicken was infected.【Conclusion】A/chicken/Guangxi/BT1/2016(H9N2),isolated from Guangxi sick chicken population,belonged to LPAIV. It is a H9N2 subtype new virus created by different subtypes AIV. It can lead to the failure of chicken vaccine immunization and has the potential risk of infecting human beings.
【Objective】This study determined molecular genetic variation regularity of H9N2 subtype avian influenza virus(AIV)and its effects on public health safety of human so as to provide reference for the prevention and control of H9 subtype avian influenza(AI).【Method】Suspected tissue samples were collected from one chicken farm in Guangxi,viral isolation was performed by inoculating SPF chicken embryo. The virus was preliminarily identified as H9 subtype AIV isolated strain. Then eight gene fragments isolated from H9 subtype AIV including HA,NA,PB2,PB,PA,NP,M and NS were sequenced,and their genetic evolution was analyzed. The biological characteristics of the isolated strain were also determined at the same time.【Result】A H9N2 subtype AIV strain,named A/chicken/Guangxi/BT1/2016(H9N2),was successfully separated from the sick chicken population in Guangxi. The cleavage site of HA gene in A/chicken/Guangxi/BT1/2016(H9N2)was PSRSSR↓GLF,which had typical features of low pathogenic AIV(LPAIV)molecule. In genetic evolution relation,it belonged to 4.2.5 branch,and had some differences with the domestic H9N2 subtype AIV vaccine strains which belonged to 4.2.3 branch. The other seven genes were also derived from different AIV subtypes. The isolated virus strain A/chicken/Guangxi/BT1/2016(H9N2)could bind to α-2,3 sialic acid and α-2,6 sialic acid receptors,with the potential risk to infect human. Virus distribution was mainly concentrated in trachea,lung and spleen after it infected chicken,and the virus also could be released through the respiratory tract and digestive tract at same time. The virus was released from day 1 and peaked on the day 5 after the chicken was infected.【Conclusion】A/chicken/Guangxi/BT1/2016(H9N2),isolated from Guangxi sick chicken population,belonged to LPAIV. It is a H9N2 subtype new virus created by different subtypes AIV. It can lead to the failure of chicken vaccine immunization and has the potential risk of infecting human beings.
引文
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仇保丰,刘武杰,胡顺林,唐应华,彭大新,刘秀梵.2010.混合感染的多种亚型禽流感病毒的纯化与鉴定[J].微生物学报,50(1):107-112.[Qiu B F,Liu W J,Hu S L,Tang Y H,Peng D X,Liu X F.2010.Purification and identification of avian influenza viruses causing mixed multi-infection[J].Acta Microbiologica Sinica,50(1):107-112.]
屈素洁,施开创,邹联斌,胡杰,张步娴,粟艳琼,梁媛,尹彦文,李军,莫胜兰.2016.2011~2014年广西H9N2亚型禽流感病毒HA基因遗传变异分析[J].畜牧与兽医,48(3):16-20.[Qu S U,Shi K C,Zou L B,Hu J,Zhang B X,Su Y Q,Liang Y,Yin Y W,Li J,Mo S L.2016.Phylogenetic analysis of HA genes of H9N2 subtype avian influenza virus in Guangxi from 2011 to 2014[J].Animal Husbandry&Veterinary Medicine,48(3):16-20.]
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王爱荣.2015.H9N2亚型AIV受体结合特性分析及内部基因对致病性影响的研究[D].泰安:山东农业大学.[Wang A R.2015.Receptor binding characterization of H9N2AIV and the relation of internal gene segments with virulence in mouse[D].Tai’an:Shandong Agricultural University.]
王秀荣,陈化兰.2015.流感病毒对人类和畜牧业健康的影响[J].中国农业科学,48(15):3038-3039.[Wang X R,Chen H L.2015.The impact of influenza viruses on human and animal husbandry[J].Scientia Agricultura Sinica,48(15):3038-3039.]
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魏宝之.2013.H9N2亚型AIV山东分离株全基因序列分析及其在豚鼠间的传播[D].泰安:山东农业大学.[Wei B Z.2013.Whole genome sequence analysis and experimental transmission in guinea pigs of H9N2 avian influenza viruses isolated from Shandong[D].Tai’an:Shandong Agricultural University.]
吴青,李银,李祥瑞,赵冬敏,刘青涛,刘宇卓,韩凯凯,黄欣梅.2017.江苏H9N2亚型禽流感病毒HA和NA的遗传进化分析[J].江西农业学报,29(7):104-109.[Wu Q,Li Y,Li X R,Zhao D M,Liu Q T,Liu Y Z,Han K K,Huang X M.2017.Genetic evolution analysis of H9N2-subtype avian influenza viruses HA and NA in Jiangsu Province[J].Acta Agriculturae Jiangxi,29(7):104-109.]
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