不同商品规格羌活药材的HPLC指纹图谱分析
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摘要
目的:建立不同商品规格羌活药材的HPLC指纹图谱,为羌活商品等级的划分和羌活药材质量的控制提供实验依据。方法:通过市场调查和文献研究,了解羌活药材商品状况,按照基原结合药材形态将羌活划分为蚕羌、条羌和大头羌3种商品规格。采用高效液相色谱-光电二极管阵列检测技术(HPLC-PDA),以乙腈-0. 3%乙酸水溶液为流动相进行梯度洗脱,建立不同商品规格羌活药材的指纹图谱;采用"中药色谱指纹图谱相似度评价系统"(2004A版)进行共有峰确认及相似度评价;通过SPSS 19. 0软件采用主成分分析对指纹图谱进行模式识别。结果:分别建立了蚕羌、条羌和大头羌3种商品规格羌活药材的指纹图谱,从蚕羌中标定了22个共有峰,条羌中标定了23个共有峰,大头羌中标定了29个共有峰。相似度评价结合主成分分析结果显示相同商品规格羌活质量较为稳定,不同商品规格羌活的化学成分组成和含量差异显著。结论:所建立的指纹图谱方法可以很好地区别不同商品规格的羌活,可为羌活商品等级的划分及其质量控制提供参考。
Objective: To establish HPLC fingerprint of Notopterygii Rhizoma et Radix with different commercial specifications and to provide the basis for the division of commercial grades and the quality control of Notopterygii Rhizoma et Radix on the market. Method: The market investigation and literature research were used to understand the existing situation of Notopterygii Rhizoma et Radix goods. Notopterygii Rhizoma et Radix goods were divided into three commercial specifications according to the source and appearance, such as Canqiang,Tiaoqiang and Datouqiang. Fingerprint of Notopterygii Rhizoma et Radix with different commercial specifications was established by HPLC-PDA,the mobile phase was consisted of acetonitrile-0. 3% acetic acid in a gradient elution mode. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( version of 2004 A) was used to confirm the common peaks and evaluate the similarity. SPSS 19. 0 statistical software was used to make principal component analysis( PCA) for HPLC fingerprint pattern. Result: The common mode of fingerprint for Canqiang,Tiaoqiang and Datouqiang were established separately. A total of 22 common peaks were marked in Canqiang,23 common peaks were marked in Tiaoqiang,29 common peaks were marked in Datouqiang.The result of similarity evaluation and PCA showed that the quality of Notopterygii Rhizoma et Radix with the same commercial specification was stable. There were great differences in chemical compositions and their contents among Notopterygii Rhizoma et Radix with different commercial specifications. Conclusion: The fingerprint method can well distinguish commercial specifications of Notopterygii Rhizoma et Radix,and it can provide the basis for the division of commercial grades and the quality control of Notopterygii Rhizoma et Radix.
Objective: To establish HPLC fingerprint of Notopterygii Rhizoma et Radix with different commercial specifications and to provide the basis for the division of commercial grades and the quality control of Notopterygii Rhizoma et Radix on the market. Method: The market investigation and literature research were used to understand the existing situation of Notopterygii Rhizoma et Radix goods. Notopterygii Rhizoma et Radix goods were divided into three commercial specifications according to the source and appearance, such as Canqiang,Tiaoqiang and Datouqiang. Fingerprint of Notopterygii Rhizoma et Radix with different commercial specifications was established by HPLC-PDA,the mobile phase was consisted of acetonitrile-0. 3% acetic acid in a gradient elution mode. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( version of 2004 A) was used to confirm the common peaks and evaluate the similarity. SPSS 19. 0 statistical software was used to make principal component analysis( PCA) for HPLC fingerprint pattern. Result: The common mode of fingerprint for Canqiang,Tiaoqiang and Datouqiang were established separately. A total of 22 common peaks were marked in Canqiang,23 common peaks were marked in Tiaoqiang,29 common peaks were marked in Datouqiang.The result of similarity evaluation and PCA showed that the quality of Notopterygii Rhizoma et Radix with the same commercial specification was stable. There were great differences in chemical compositions and their contents among Notopterygii Rhizoma et Radix with different commercial specifications. Conclusion: The fingerprint method can well distinguish commercial specifications of Notopterygii Rhizoma et Radix,and it can provide the basis for the division of commercial grades and the quality control of Notopterygii Rhizoma et Radix.
引文
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[8]宋九华,杨文钰,陈兴福,等. HPLC指纹图谱结合灰色关联度分析综合评价云南丽江秦艽资源[J].化学研究与应用,2014,26(8):1249-1254.
[9]张藏蔓,卢敬光,谢启师,等.基于UHPLC-TOF-MS技术建立金线莲的指纹图谱及金线莲苷的含量测定方法[J].中国实验方剂学杂志,2018,24(1):31-37.
[10] WANG Y P,HUANG L F. Comparison of two species of Notopterygium by GC-MS and HPLC[J]. Molecules,2015,20(3):5062-5073.
[11]朱美晓.羌活药材质量标准研究[D].泸州:泸州医学院,2010.
[12] LI Y H,JIANG S Y,GUAN Y L,et al. Quantitative determination of the chemical profile of the plant material “Qiang-huo” by LC-ESI-MS-MS[J].Chromatographia,2006,64(7/8):405-411.
[2]李石平,沙龙,赵祎武,等.近30年来中药羌活化学成分研究进展[J].中国中药杂志,2015,40(15):2952-2963.
[3]刘卫根,王亮生,徐文华,等.不同商品等级羌活中有机酸和香豆素类化合物的测定[J].中成药,2012,34(11):2181-2186.
[4]刘卫根,周国英,徐文华,等.不同商品等级羌活挥发油的比较研究[J].中药材,2012,35(7):1042-1045.
[5]蒋舜媛,孙洪兵,孙辉,等.羌活药材商品规格与质量等级的对应分析[J].中国中药杂志,2016,41(5):793-801.
[6]陈虹宇,尹显梅,陈玲,等.不同商品等级羌活中羌活醇和异欧前胡素的含量测定[J].成都中医药大学学报,2016,39(1):18-21.
[7]陈小红,陈康,潘超美,等.化橘红药材商品规格等级标准分析[J].中国实验方剂学杂志,2017,23(11):23-28.
[8]宋九华,杨文钰,陈兴福,等. HPLC指纹图谱结合灰色关联度分析综合评价云南丽江秦艽资源[J].化学研究与应用,2014,26(8):1249-1254.
[9]张藏蔓,卢敬光,谢启师,等.基于UHPLC-TOF-MS技术建立金线莲的指纹图谱及金线莲苷的含量测定方法[J].中国实验方剂学杂志,2018,24(1):31-37.
[10] WANG Y P,HUANG L F. Comparison of two species of Notopterygium by GC-MS and HPLC[J]. Molecules,2015,20(3):5062-5073.
[11]朱美晓.羌活药材质量标准研究[D].泸州:泸州医学院,2010.
[12] LI Y H,JIANG S Y,GUAN Y L,et al. Quantitative determination of the chemical profile of the plant material “Qiang-huo” by LC-ESI-MS-MS[J].Chromatographia,2006,64(7/8):405-411.