人参皂苷CK对重症急性胰腺炎大鼠肝功能和免疫作用的影响
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摘要
目的探讨人参皂苷CK对重症急性胰腺炎(SAP)大鼠肝功能和免疫作用的影响。方法将46只SAP大鼠随机分为实验组和对照组各23只,对照组采用常规肠内营养和治疗方案,实验组在常规治疗方案基础上采用人参皂苷CK,观察两组大鼠肝功能变化和对免疫功能的影响。结果两组SAP大鼠治疗3、7 d后分别检测血白细胞计数、肝功能(谷丙转氨酶、谷草转氨酶和谷氨酰转肽酶、白蛋白)和免疫功能T细胞亚群(CD_3、CD_4和CD_8阳性细胞数及CD_4/CD_8比值)变化,实验组各项指标均优于对照组(P<0.05)。结论人参皂苷CK作为治疗SAP的辅助手段,具有肝脏保护作用和提高免疫功能。
Objective To study the effect of the ginsenoside compound K( CK) on the function of liver and immunity in rats with severe acute pancreatitis( SAP). Methods Twenty-three rats with severe acute pancreatitis were treated by ginsenoside CK as the experimental group and 23 rats in the control group received normal SAP treatment. The changes of liver function indexes( AST,ALT,GGT and ALB) and immunity function indexes such as T cell( CD_3,CD_4,CD_8 cell and CD_4/CD_8) after the SAP rats received treatment in 3 days and 7 days were determined. Results Compared with the control group,the plasma liver function index( AST,ALT,GGT,ALB and STB) levels and the immunity T cell function index( CD_3,CD_4,CD_8 cell and CD_4/CD_8) levels were increased in 3 days and 7 days and there was significant difference( P < 0. 05). Conclusion Ginsenoside CK therapy can effectively protect the function of the liver and immunity in rats with severe acute pancreatitis,and also improve their overall condition. It was an important auxiliary treatment for SAP rats,and it's worthy of deep research in the future.
Objective To study the effect of the ginsenoside compound K( CK) on the function of liver and immunity in rats with severe acute pancreatitis( SAP). Methods Twenty-three rats with severe acute pancreatitis were treated by ginsenoside CK as the experimental group and 23 rats in the control group received normal SAP treatment. The changes of liver function indexes( AST,ALT,GGT and ALB) and immunity function indexes such as T cell( CD_3,CD_4,CD_8 cell and CD_4/CD_8) after the SAP rats received treatment in 3 days and 7 days were determined. Results Compared with the control group,the plasma liver function index( AST,ALT,GGT,ALB and STB) levels and the immunity T cell function index( CD_3,CD_4,CD_8 cell and CD_4/CD_8) levels were increased in 3 days and 7 days and there was significant difference( P < 0. 05). Conclusion Ginsenoside CK therapy can effectively protect the function of the liver and immunity in rats with severe acute pancreatitis,and also improve their overall condition. It was an important auxiliary treatment for SAP rats,and it's worthy of deep research in the future.
引文
[1]中华医学会消化病学分会胰腺疾病学组.中国急性胰腺炎诊治指南[J].中国实用内科杂志,2013,33(7):530-534.
[2]杨元超,王英平,闫梅霞,等.人参皂苷compound K转化菌株的筛选[J].中国中药杂志,2011,36(12):1596-1598.
[3]于雷,李成龙,于珊珊.人参皂苷CK的研究进展[J].生物技术进展,2013,22(1):31-35.
[4]Joh E H,Lee I A,Jung I H,et al.Ginsenoside Rb1 and its metabolite compound K inhibit IRAK-1 activation-The key step of inflammation[J].Biochem pharmacol,2011,82(8):278-286.
[5]Wang C Z,Du G J,Zhang Z,et al.Ginsenoside compound K,not Rb1,possesses potential chemopreventive activities in human colorectal cancer[J].Int J Oncol,2012,40(6):1970-1976.
[6]Park J S,Shin J A,Jung J S,et al.Anti-inflammatory mechanism of compound k in activated microglia and its neuroprotective effect on experimental stroke in mice[J].J Pharmacol Exp Ther,2012,341(1):59-67.
[7]张改玲,葛相栓,李建平,等.急性胰腺炎肝功能损伤的临床意义分析[J].中国实用医药,2015,10(20):46-47.
[8]徐彦哲,丁佑铭,汪斌,等.急性胰腺炎肝损伤的研究进展[J].中华肝胆外科杂志,2015,21(4):284-288.
[9]彭婷婷,李硕,冯怀志,等.早期免疫微生态肠内营养治疗重症急性胰腺炎的疗效分析[J].西南国防医药,2015,25(2):180-182.
[10]冯丹.人参皂苷用于肝脏疾病的研究进展[J].中国现代应用药学,2016,33(5):671-675.
[11]刘荣荣,张璇,肖斌,等.人参皂苷CK诱导人肝癌Hep G-2细胞凋亡的研究[J].时珍国医国药,2016,27(4):843-845.
[12]崔江河,韩光宇,何光美,等.人参皂苷CK联合5-氟尿嘧啶对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化的影响[J].中国药房,2017,28(31):4388-4392.
[13]刘博,王玉梅,周少英.强化谷氨酰胺的肠内营养联合微生态制剂对重症急性胰腺炎患者临床预后的影响[J].现代中西医结合杂志,2017,26(17):1838-1841.
[14]张春晶,李淑艳,赵容杰.人参皂苷Rh1对哮喘模型小鼠炎症因子表达的抑制作用[J].中国病理生理杂志,2018,34(1):163-167.
[2]杨元超,王英平,闫梅霞,等.人参皂苷compound K转化菌株的筛选[J].中国中药杂志,2011,36(12):1596-1598.
[3]于雷,李成龙,于珊珊.人参皂苷CK的研究进展[J].生物技术进展,2013,22(1):31-35.
[4]Joh E H,Lee I A,Jung I H,et al.Ginsenoside Rb1 and its metabolite compound K inhibit IRAK-1 activation-The key step of inflammation[J].Biochem pharmacol,2011,82(8):278-286.
[5]Wang C Z,Du G J,Zhang Z,et al.Ginsenoside compound K,not Rb1,possesses potential chemopreventive activities in human colorectal cancer[J].Int J Oncol,2012,40(6):1970-1976.
[6]Park J S,Shin J A,Jung J S,et al.Anti-inflammatory mechanism of compound k in activated microglia and its neuroprotective effect on experimental stroke in mice[J].J Pharmacol Exp Ther,2012,341(1):59-67.
[7]张改玲,葛相栓,李建平,等.急性胰腺炎肝功能损伤的临床意义分析[J].中国实用医药,2015,10(20):46-47.
[8]徐彦哲,丁佑铭,汪斌,等.急性胰腺炎肝损伤的研究进展[J].中华肝胆外科杂志,2015,21(4):284-288.
[9]彭婷婷,李硕,冯怀志,等.早期免疫微生态肠内营养治疗重症急性胰腺炎的疗效分析[J].西南国防医药,2015,25(2):180-182.
[10]冯丹.人参皂苷用于肝脏疾病的研究进展[J].中国现代应用药学,2016,33(5):671-675.
[11]刘荣荣,张璇,肖斌,等.人参皂苷CK诱导人肝癌Hep G-2细胞凋亡的研究[J].时珍国医国药,2016,27(4):843-845.
[12]崔江河,韩光宇,何光美,等.人参皂苷CK联合5-氟尿嘧啶对人胰腺癌PANC-1细胞的增殖、凋亡及上皮间质转化的影响[J].中国药房,2017,28(31):4388-4392.
[13]刘博,王玉梅,周少英.强化谷氨酰胺的肠内营养联合微生态制剂对重症急性胰腺炎患者临床预后的影响[J].现代中西医结合杂志,2017,26(17):1838-1841.
[14]张春晶,李淑艳,赵容杰.人参皂苷Rh1对哮喘模型小鼠炎症因子表达的抑制作用[J].中国病理生理杂志,2018,34(1):163-167.