新生儿呼吸道标本采集技术及微生态监测技术研究进展及其临床应用
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摘要
上下呼吸道中细菌、真菌、病毒、支原体、衣原体等全部微生物构建成人体呼吸道微生态,与人体细胞共同组成"超级有机体"。随着呼吸道标本采集技术和高通量测序等技术的应用,往日阻碍人体微生态研究的瓶颈打开,借助这些新技术可深入了解新生儿呼吸道中的微生物群落的组成、功能及呼吸道微生态与人体健康和疾病的关系,为预防和治疗呼吸道疾病提供了新生的研究途径。
Bacteria, fungi, viruses, mycoplasma, chlamydia and other microorganisms in upper and lower respiratory tracts construct the human respiratory microecology, which forms a "superorganism" together with human cells. With the application of respiratory specimens collection technology and high-throughput sequencing technology, the bottleneck of human microecology research in the past has been opened. With the help of these new technologies, the composition and function of microecology in neonatal respiratory tract and the relationship between respiratory microecology and human health of diseases can be deeply understood, which provides a new research approach for the prevention and treatment of respiratory diseases.
Bacteria, fungi, viruses, mycoplasma, chlamydia and other microorganisms in upper and lower respiratory tracts construct the human respiratory microecology, which forms a "superorganism" together with human cells. With the application of respiratory specimens collection technology and high-throughput sequencing technology, the bottleneck of human microecology research in the past has been opened. With the help of these new technologies, the composition and function of microecology in neonatal respiratory tract and the relationship between respiratory microecology and human health of diseases can be deeply understood, which provides a new research approach for the prevention and treatment of respiratory diseases.
引文
[1]陈海为,苏宁,温建华.吸痰方法与呼吸机相关性肺炎发生率关系的系统综述[J].中国医药指南,2017,15(19):49-52.
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[6]方秀丽.密闭式吸痰方法在机械通气新生儿中的应用[J].当代护士:学术版旬刊,2017(2):74-75.
[7]郭琳惠.氧气雾化器结合密闭式吸痰管应用于人工气道护理中的效果分析[J].基层医学论坛,2016,20(18):2603-2604.
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[15]张莉,杨持,其木格,等.新生儿呼吸道微生态平衡动态研究[J].中国公共卫生,2007,23(11):1305-1306.
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[17]邱炳峰,张晓飞,顾丹霞,等.VITEK MS基质辅助激光解析电离飞行时间质谱在病原菌鉴定中的应用评价[J].中华医院感染学杂志,2016,26(10):2199-2202.
[18]张霄霄,汪定成,戈伟,等.MALDI-TOF-MS技术在酵母样真菌鉴定中的临床应用评价[J].中国真菌学杂志,2016,11(5):285-288.
[19]周宋元,黄颖,徐元宏.MALDI-TOFMS技术在鉴定临床少见非发酵菌中的应用[J].中国现代医学杂志,2017,27(9):69-73.
[20]程敬伟,王贺,徐英春.基质辅助激光解吸附电离飞行时间质谱在临床病毒学中的应用[J].临床检验杂志,2014,32(9):685-686.
[21]秦娟秀,李敏.MALDI-TOFMS指纹图谱技术在病原微生物分型中的应用前景[J].中华检验医学杂志,2015,38(6):367-369.
[22]李修远,黄艳飞,尚军,等.MALDI-TOF MS在临床微生物鉴定中的常见问题及对策[J].临床检验杂志,2016,34(12):885-891.
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[24]张存,周晓玉.新生儿呼吸道微生态与疾病的关系[J].中华实用儿科临床杂志,2017,32(17):1358-1360.
[25]LILES MR,MANSKE BF,BINTRIM SB,et al.A census of rRNAgenes and linked genomic sequences within a soil metagenomic library[J].Appl Environ Microbiol,2003,69(5):2684-2691.
[26]陆韦,余加林,艾青,等.细菌感染性肺炎新生儿呼吸道菌群研究[J].现代医药卫生,2017,33(10):1444-1446.
[27]宋超,余加林,艾青,等.机械通气新生儿气管导管表面生物膜细菌多样性研究[J].中华儿科杂志,2013,51(8):602-607.
[28]沈德新,杜江,封志纯.新生儿常见感染病原菌的16SrDNA寡核苷酸阵列快速诊断[J].中华儿科杂志,2004,42(9):668-672.
[29]陈雄毅,岑丽莲,申学基,等.肺炎支原体、衣原体感染与新生儿肺炎的关系研究[J].国际检验医学杂志,2017,38(13):1847-1848.
[30]REID AL,FREEMAN JB,MILLWARD M,et al.Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR[J].Clinical Bio-chemistry,2014,48,(15):999-1002.
[31]RICCHI M,BERTASIO C,BONIOTTI M B,et al.Comparison among the quantification of bacterial pathogens by qpcr,dpcr,and cultural methods[J].Front Microbiol,2017,8(6):1174-1189.
[32]AAGAARD K,MA J,ANTONY KM,et al.the placenta harbors a unique microbiome[J].Sci Transl Med,2014,6(237):237-265.
[33]HEDLUND M,ASCHENBRENNER LM,JENSEN K,et al.Sialidasebased anti-influenza virus therapy protects against secondary pneumo-coccal infection[J].J Infect Dis,2010,201(7):1007-1015.
[2]BLOOM B,CRADDOCK A,DELMORE P,et al.Reducing acquired infection inthe NICU:observing and implementing meaningful differences in processbetween high and low acquired infection rate centers[J].J Perinatol,2003,23(6):489-492.
[3]CLARK R,POWERS R,WHITE R,et al.Prevention and treatment of nosocomialsepsis in the NICU[J].J Perinatol,2004,24(7):446-453.
[4]SIEMPOS II,VARDAKAS KZ,FALAGAS ME.Closed tracheal suctionsystems for prevention of ventilator-associated pneumonia[J].Br JAnaesth,2008,100(3):299-306.
[5]袁继红,张漪,夏世文.密闭式吸痰管对新生儿机械通气疗效及并发症的影响[J].中国新生儿科杂志,2012,27(6):401-403.
[6]方秀丽.密闭式吸痰方法在机械通气新生儿中的应用[J].当代护士:学术版旬刊,2017(2):74-75.
[7]郭琳惠.氧气雾化器结合密闭式吸痰管应用于人工气道护理中的效果分析[J].基层医学论坛,2016,20(18):2603-2604.
[8]PREBIL SE,ANDREWS J,CRIBBS SK,et al.Safety of research bronchoscopy in critically ill patients[J].J Crit Care,2014,29(6):961-964.
[9]梁咏雪,黄锐,周锦妍,等.纤维支气管镜在早产极低体重儿呼吸道疾病诊治中的临床应用[J].中国妇幼保健,2016,31(3):651-653.
[10]牛红艳,萨初然贵,胡景伟,等.赤峰地区小儿肺部感染支气管灌洗液细菌培养结果分析[J].内蒙古医科大学学报,2017,39(4):330-333,337.
[11]陈志敏.支气管肺泡灌洗在儿童肺部感染病原学诊断中的应用[J].中华儿科杂志,2010,48(10):753-755.
[12]陈敏.电子支气管镜术在小儿呼吸道疾病诊治中的应用及安全性研究[D].贵阳:贵阳医学院,2014.
[13]王柱,张小庄,黄冬平,等.电子支气管镜在婴幼儿呼吸困难疾病诊治中的应用价值[J].中国当代儿科杂志,2015,17(7):747-750.
[14]黄国盛,黄惠萍,廖燕,等.电子支气管镜在新生儿呼吸道疾病的临床应用研究[J].中国医学创新,2014,7(9):154-156.
[15]张莉,杨持,其木格,等.新生儿呼吸道微生态平衡动态研究[J].中国公共卫生,2007,23(11):1305-1306.
[16]MAN WH,WA DSP,BOGAERT D.The microbiota of the respiratory tract:gatekeeper to respiratory health[J].Nat Rev Microbiol,2017,15(5):259.
[17]邱炳峰,张晓飞,顾丹霞,等.VITEK MS基质辅助激光解析电离飞行时间质谱在病原菌鉴定中的应用评价[J].中华医院感染学杂志,2016,26(10):2199-2202.
[18]张霄霄,汪定成,戈伟,等.MALDI-TOF-MS技术在酵母样真菌鉴定中的临床应用评价[J].中国真菌学杂志,2016,11(5):285-288.
[19]周宋元,黄颖,徐元宏.MALDI-TOFMS技术在鉴定临床少见非发酵菌中的应用[J].中国现代医学杂志,2017,27(9):69-73.
[20]程敬伟,王贺,徐英春.基质辅助激光解吸附电离飞行时间质谱在临床病毒学中的应用[J].临床检验杂志,2014,32(9):685-686.
[21]秦娟秀,李敏.MALDI-TOFMS指纹图谱技术在病原微生物分型中的应用前景[J].中华检验医学杂志,2015,38(6):367-369.
[22]李修远,黄艳飞,尚军,等.MALDI-TOF MS在临床微生物鉴定中的常见问题及对策[J].临床检验杂志,2016,34(12):885-891.
[23]GONG YN,YANG SL,CHEN GW,et al.A metagenomics study for the identification of respiratory viruses in mixed clinical specimens:an application of the iterative mapping approach[J].Arch Virol,2017,162(7):2003-2012.
[24]张存,周晓玉.新生儿呼吸道微生态与疾病的关系[J].中华实用儿科临床杂志,2017,32(17):1358-1360.
[25]LILES MR,MANSKE BF,BINTRIM SB,et al.A census of rRNAgenes and linked genomic sequences within a soil metagenomic library[J].Appl Environ Microbiol,2003,69(5):2684-2691.
[26]陆韦,余加林,艾青,等.细菌感染性肺炎新生儿呼吸道菌群研究[J].现代医药卫生,2017,33(10):1444-1446.
[27]宋超,余加林,艾青,等.机械通气新生儿气管导管表面生物膜细菌多样性研究[J].中华儿科杂志,2013,51(8):602-607.
[28]沈德新,杜江,封志纯.新生儿常见感染病原菌的16SrDNA寡核苷酸阵列快速诊断[J].中华儿科杂志,2004,42(9):668-672.
[29]陈雄毅,岑丽莲,申学基,等.肺炎支原体、衣原体感染与新生儿肺炎的关系研究[J].国际检验医学杂志,2017,38(13):1847-1848.
[30]REID AL,FREEMAN JB,MILLWARD M,et al.Detection of BRAF-V600E and V600K in melanoma circulating tumour cells by droplet digital PCR[J].Clinical Bio-chemistry,2014,48,(15):999-1002.
[31]RICCHI M,BERTASIO C,BONIOTTI M B,et al.Comparison among the quantification of bacterial pathogens by qpcr,dpcr,and cultural methods[J].Front Microbiol,2017,8(6):1174-1189.
[32]AAGAARD K,MA J,ANTONY KM,et al.the placenta harbors a unique microbiome[J].Sci Transl Med,2014,6(237):237-265.
[33]HEDLUND M,ASCHENBRENNER LM,JENSEN K,et al.Sialidasebased anti-influenza virus therapy protects against secondary pneumo-coccal infection[J].J Infect Dis,2010,201(7):1007-1015.