复方克林霉素脂质体凝胶的制备及体外透皮释药研究
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摘要
目的制备复方克林霉素脂质体凝胶并考察其粒径分布、包封率及体外透皮特性。方法采用薄膜分散法制备复方克林霉素脂质体,利用透射电镜观察其形态,用激光纳米粒度仪测定其粒径大小及分布,用HPLC法测定其包封率。将脂质体进一步制成凝胶剂后,考察其体外透皮情况。结果复方克林霉素脂质体的粒径在240nm左右,分布均匀,平均包封率为51.24%;脂质体中的克林霉素能缓慢透过大鼠皮肤,缓释效果明显。结论该制剂制备工艺简单,性质稳定,药物包封率较高,定量测定方法简便、准确;药物透皮速率缓慢,释药稳定。
Objective To prepare Compound Clindamycin Liposome Gel and investigate its particle size distribution,encapsulation rate and in vitro transdermal properties.Methods Compound clindamycin liposomes were prepared by thin film dispersing method,their morphology was observed by radio microscope,particle size and distribution were determined by laser nanometer granulometer,and the encapsulation rate was determined by HPLC.After the liposomes were further prepared into gels,the skin penetration in vitro was investigated.Results The particle size of compound clindamycin liposomes was evenly distributed,and the particle size was about 240 nm,with an average encapsulation rate of 51.24%.Clindamycin in liposomes can pass through the skin of rats slowly and release slowly.Conclusion The preparation process is simple,the property is stable,the drug encapsulation rate is high,the quantitative determination method is simple and accurate.Drug penetration rate is slow and drug release is stable.
Objective To prepare Compound Clindamycin Liposome Gel and investigate its particle size distribution,encapsulation rate and in vitro transdermal properties.Methods Compound clindamycin liposomes were prepared by thin film dispersing method,their morphology was observed by radio microscope,particle size and distribution were determined by laser nanometer granulometer,and the encapsulation rate was determined by HPLC.After the liposomes were further prepared into gels,the skin penetration in vitro was investigated.Results The particle size of compound clindamycin liposomes was evenly distributed,and the particle size was about 240 nm,with an average encapsulation rate of 51.24%.Clindamycin in liposomes can pass through the skin of rats slowly and release slowly.Conclusion The preparation process is simple,the property is stable,the drug encapsulation rate is high,the quantitative determination method is simple and accurate.Drug penetration rate is slow and drug release is stable.
引文
[1]范铭,彭雯艳,沈春英.克林霉素致不良反应文献分析[J].医药导报,2010,29(7):963-965.
[2]辛艳丽,齐晓涟,陈莲珍,等.克林霉素注射剂临床应用安全性调查与评价[J].中国药房,2005,16(23):1804-1806.
[3]邓玲,邓盛齐.克林霉素国内外制剂的研究进展[J].西南国防医药,2006,16(2):222-224.
[4]李蕊,王梅.去氢骆驼蓬碱长循环磁纳米脂质体的制备及体外性质的研究[J].西北药学杂志,2017,32(2):181-184.
[5]张祺照.脂质体制备方法研究概况[J].亚太传统医药,2013,9(12):71-72.
[6]孙庆雪,邵伟,黄桂华.脂质体制备方法的选择[J].中成药,2010,32(8):1397-1401.
[7]张慧慧,刘超英.三七叶总皂苷脂质体凝胶剂的制备及其离体透皮率考察[J].中国实验方剂学杂志,2013,19(21):45-48.
[8]曾昭武,王小丽.苦参碱醇质体的制备[J].中国组织工程研究与临床康复,2008,12(41):8107-8110.
[9]郭咸希,何杨虎,何文,等.尼美舒利脂质体凝胶的研制及体外经皮渗透动力学考察[J].中国药学杂志,2008,43(1):35-39.
[10]刘辉,吴菌子,盛利.皮肤用脂质体的研究[J].药学进展,2000,24(3):141-144.
[11]梁键谋,王知坚,阮昊,等.HPLC梯度洗脱法检测克林霉素磷酸酯及其注射剂的有关物质[J].中国现代应用药学,2012,29(8):726-730.
[12]倪冬胜,左从菊,李小羿,等.HPLC法测定复方阿达帕林盐酸克林霉素凝胶中盐酸克林霉素有关物质[J].药物分析杂志,2018,38(1):135-142.
[13]韩文霞,李伟泽,汪兴军,等.盐酸青藤碱纳米柔性脂质体的制备及其性质研究[J].中草药,2011,42(4):671-675.
[14]时军,王芳,滕希峰,等.丹皮酚阳离子脂质体凝胶剂的稳定性及离体皮肤渗透试验研究[J].中药材,2012,35(5):803-807.
[15]张爱军,封新,马小亚,等.辣椒素脂质体凝胶剂的制备与释放度的测定[J].西北药学杂志,2011,26(4):279-280.
[16]刘晓谦,王锦玉,仝燕,等.脂质体制备技术及其研究进展[J].中国药学杂志,2011,46(14):1084-1088.
[17]芦洁,祝林,金鸿莱,等.盐酸多西环素脂质体凝胶剂的制备与评价[J].中国医药工业杂志,2006,37(2):93-95.
[18]李秀英,曾凡,赵曜,等.脂质体药物递送系统的研究进展[J].中国新药杂志,2014,23(16):1904-1911,1917.
[19]王鹏,杨秀丽,杨波,等.紫杉醇脂质体凝胶剂的制备及其镇痛抗炎作用[J].中国现代应用药学,2015,32(3):281-285.
[20]张小灵,郭嘉俊,时军,等.双丹脂质体凝胶剂的制备工艺优化及体外透皮性能研究[J].中药材,2017,40(2):402-407.
[2]辛艳丽,齐晓涟,陈莲珍,等.克林霉素注射剂临床应用安全性调查与评价[J].中国药房,2005,16(23):1804-1806.
[3]邓玲,邓盛齐.克林霉素国内外制剂的研究进展[J].西南国防医药,2006,16(2):222-224.
[4]李蕊,王梅.去氢骆驼蓬碱长循环磁纳米脂质体的制备及体外性质的研究[J].西北药学杂志,2017,32(2):181-184.
[5]张祺照.脂质体制备方法研究概况[J].亚太传统医药,2013,9(12):71-72.
[6]孙庆雪,邵伟,黄桂华.脂质体制备方法的选择[J].中成药,2010,32(8):1397-1401.
[7]张慧慧,刘超英.三七叶总皂苷脂质体凝胶剂的制备及其离体透皮率考察[J].中国实验方剂学杂志,2013,19(21):45-48.
[8]曾昭武,王小丽.苦参碱醇质体的制备[J].中国组织工程研究与临床康复,2008,12(41):8107-8110.
[9]郭咸希,何杨虎,何文,等.尼美舒利脂质体凝胶的研制及体外经皮渗透动力学考察[J].中国药学杂志,2008,43(1):35-39.
[10]刘辉,吴菌子,盛利.皮肤用脂质体的研究[J].药学进展,2000,24(3):141-144.
[11]梁键谋,王知坚,阮昊,等.HPLC梯度洗脱法检测克林霉素磷酸酯及其注射剂的有关物质[J].中国现代应用药学,2012,29(8):726-730.
[12]倪冬胜,左从菊,李小羿,等.HPLC法测定复方阿达帕林盐酸克林霉素凝胶中盐酸克林霉素有关物质[J].药物分析杂志,2018,38(1):135-142.
[13]韩文霞,李伟泽,汪兴军,等.盐酸青藤碱纳米柔性脂质体的制备及其性质研究[J].中草药,2011,42(4):671-675.
[14]时军,王芳,滕希峰,等.丹皮酚阳离子脂质体凝胶剂的稳定性及离体皮肤渗透试验研究[J].中药材,2012,35(5):803-807.
[15]张爱军,封新,马小亚,等.辣椒素脂质体凝胶剂的制备与释放度的测定[J].西北药学杂志,2011,26(4):279-280.
[16]刘晓谦,王锦玉,仝燕,等.脂质体制备技术及其研究进展[J].中国药学杂志,2011,46(14):1084-1088.
[17]芦洁,祝林,金鸿莱,等.盐酸多西环素脂质体凝胶剂的制备与评价[J].中国医药工业杂志,2006,37(2):93-95.
[18]李秀英,曾凡,赵曜,等.脂质体药物递送系统的研究进展[J].中国新药杂志,2014,23(16):1904-1911,1917.
[19]王鹏,杨秀丽,杨波,等.紫杉醇脂质体凝胶剂的制备及其镇痛抗炎作用[J].中国现代应用药学,2015,32(3):281-285.
[20]张小灵,郭嘉俊,时军,等.双丹脂质体凝胶剂的制备工艺优化及体外透皮性能研究[J].中药材,2017,40(2):402-407.