仙人掌多糖发酵提取工艺优化及其抗炎功效研究
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摘要
本文分别采用乳酸菌与酵母菌发酵提取仙人掌多糖,并探究仙人掌多糖的抗炎功效。在三个单因素液料比、时间、pH方面探究仙人掌多糖的提取工艺,得到三因素最适组合,乳酸菌发酵最适提取工艺为发酵时间7 h、液料比25∶1 (mL/g)、pH为5,此工艺下多糖得率为0.26%。黄酒酵母发酵最适提取工艺为发酵时间48 h、液料比25∶1 (mL/g)、pH为3,此工艺下多糖得率为0.53%。应用实时荧光定量PCR法检测仙人掌多糖对IL-6、IL-8炎症细胞因子相关基因表达的影响,得知仙人掌多糖通过抑制IL-8的表达,促进IL-6的表达来抑制炎症的发生和发展。
Using lactic acid bacteria and yeast fermentation to extract Opuntia dillenii polysaccharide,and explored the antiinflammatory effects of ODP.The extraction process of ODP was investigated in three single-factor: ratios of liquid to material,time and pH.The best combination of the three factors was obtained.Design expert analysis indicated that the optimal processing conditions of Lactobacillus were: time 7 hours,liquid to solid ratio 25∶ 1 (mL/g),pH5,under these conditions,the extraction yield of ODP was 0.26%.The optimal processing conditions of Yellow yeast fermentation were: time 48 hours,liquid to solid ratio 25∶ 1 (mL/g),pH3,under these conditions,the extraction yield of ODP was 0.53%.The effect of ODP on IL-6 and IL-8 expression in inflammatory cytokines was examined by real-time fluorescence quantitative PCR. It was found that ODP could affect the inflammatory response by up-regulated IL-6 and repressed IL-8 to inhibit the occurrence and development of inflammation.
Using lactic acid bacteria and yeast fermentation to extract Opuntia dillenii polysaccharide,and explored the antiinflammatory effects of ODP.The extraction process of ODP was investigated in three single-factor: ratios of liquid to material,time and pH.The best combination of the three factors was obtained.Design expert analysis indicated that the optimal processing conditions of Lactobacillus were: time 7 hours,liquid to solid ratio 25∶ 1 (mL/g),pH5,under these conditions,the extraction yield of ODP was 0.26%.The optimal processing conditions of Yellow yeast fermentation were: time 48 hours,liquid to solid ratio 25∶ 1 (mL/g),pH3,under these conditions,the extraction yield of ODP was 0.53%.The effect of ODP on IL-6 and IL-8 expression in inflammatory cytokines was examined by real-time fluorescence quantitative PCR. It was found that ODP could affect the inflammatory response by up-regulated IL-6 and repressed IL-8 to inhibit the occurrence and development of inflammation.
引文
[1]林款,徐丛玥,梁征,等.米邦塔仙人掌多糖的提取纯化及体外抗氧化活性[J].食品工业科技,2017(5):140-150.
[2]陶美华,曾富华,卢向阳,等.仙人掌多糖的降血糖作用[J].湖南农业大学学报:自然科学版,2015,31(6):612-615.
[3]李春艳,成小松,崔美芝,等.仙人掌粉对大鼠血脂的调节作用及其机制的探讨[J].中国中药杂志,2005,50(9):694-696.
[4]喻宁华,曾富华,饶力群.仙人掌多糖对小鼠急性肝损伤的保护作用[J].中国生化药物杂志,2009,30(4):255-258.
[5]林爱琴,方幼兰,林曦,等.仙人掌多糖部分理化性质与抗肿瘤活性的研究[J].福建师大福清分校学报,2007,26(2):19-21.
[6]蔡为荣,谢亮亮,汤坚.仙人掌多糖结构分析及抗凝血活性研究[J].安徽农业科学,2012,40(4):2310-2314.
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[10]高南南,杨润梅,俞东平,等.仙人掌主要药效学研究[N].中医药学,2004,32(6):12.
[11]G Franz.Polysaccharides in pharmacy applications and future concepts[J].Plant Medica,1989,55:494-498.
[12]唐馄,谢小慧,陈志达,等.仙人掌多糖对大鼠局灶性脑缺血的神经保护作用[J].医药导报,2012,31(9):1109-1110.
[13]郑海平,欧超,曹骥,等.仙人掌多糖主要组分成分分析及其对体外培养免疫细胞的影响[J].时珍国医国药,2012,23(7):1653-1656.
[14]虞旦,谢娅霏,苏宁,等.新鲜仙人掌多糖的提取及其抗氧化活性研究[J].食品研究与开发,2017,38(19):29-35.
[15]郭利平.仙人掌多糖的分离纯化、结构和生物活性研究进展[J].中国食品科添加剂(专论综述),2010:185-189.
[16]马世宏,单承莺,陈兴芬,等.仙人掌提取物在化妆品中的应用[J].日用化学品科学,2009,32(12):29-33.
[17]巴媛媛,王莹,朴美子.苯酚-硫酸法测定瓦尼木层孔菌菌丝体多糖含量的条件优化[J].食品工业科技,2011,32(5):389-391.
[18]蒋艳玲,赵明,梁功平,等.1,25-二羟维生素D3对Hacat细胞细胞增殖活性及基因组DNA和增殖相关基因启动子甲基化水平的影响[J].中华皮肤科杂志,2013,46(12):885-888.
[19]杨井,陶娟,李延,等.缺氧对Hacat细胞细胞HIF-1α,GLUT-1表达的影响及与细胞增殖的关系[J].中国皮肤性病学杂志,2009,23(10):621-623.
[20]白雨鑫,郭斌,韩冠英,等.萝藦果壳多糖提取工艺优化及其抗氧化活性研究[J].食品工业科技,2015,36(20);278-283.
[21]熊素玉,姚新奎,谭小海,等.不同温度及p H条件对乳酸菌生长影响的研究[J].新疆农业科学,2006,43(6):533-538.
[22]虞旦,谢娅霏,苏宁,等.新鲜仙人掌多糖的提取及其抗氧化活性研究[J].食品研究与开发,2017,38(19):29-34.
[23]Tcherakian C,Rivaud E,Catherinot E,et al.Pulmonary arterial hypertension related to HIV:is inflammation related to IL-6 the cornerstone?[J].Revue de Pneumologie Clinique,2011,67(4):250-257.
[24]杨敏,黄凤楼,傅海涛,等.原发性胆汁性肝硬化患者外周血IL-8及其受体CXCR1、CXCR2的表达及临床意义[J].临床实验诊断研究,2014:362-363.
[2]陶美华,曾富华,卢向阳,等.仙人掌多糖的降血糖作用[J].湖南农业大学学报:自然科学版,2015,31(6):612-615.
[3]李春艳,成小松,崔美芝,等.仙人掌粉对大鼠血脂的调节作用及其机制的探讨[J].中国中药杂志,2005,50(9):694-696.
[4]喻宁华,曾富华,饶力群.仙人掌多糖对小鼠急性肝损伤的保护作用[J].中国生化药物杂志,2009,30(4):255-258.
[5]林爱琴,方幼兰,林曦,等.仙人掌多糖部分理化性质与抗肿瘤活性的研究[J].福建师大福清分校学报,2007,26(2):19-21.
[6]蔡为荣,谢亮亮,汤坚.仙人掌多糖结构分析及抗凝血活性研究[J].安徽农业科学,2012,40(4):2310-2314.
[7]Huang X J,Li Q,Guo L J,et al.Protection of cactus polysaccharide against H2O2-induced damage in the rat cerebral cortex and hippocampus differences in time of administration[J].Neurol Regener Research,2008,3(1):14-18.
[8]Huang X J,Li Q,Zhang Y P,et al.Neuroprotective effects o cactus polysaccharide on oxygen and glucose deprivation induced damage in rat brain slices[J].Cellular and Molecular Neurobiology,2008,28:559-568.
[9]杨洋,刘翀,覃记杰,等.仙人掌提取物的抑菌作用[J].食品工业科技,2003,24(5):40-42.
[10]高南南,杨润梅,俞东平,等.仙人掌主要药效学研究[N].中医药学,2004,32(6):12.
[11]G Franz.Polysaccharides in pharmacy applications and future concepts[J].Plant Medica,1989,55:494-498.
[12]唐馄,谢小慧,陈志达,等.仙人掌多糖对大鼠局灶性脑缺血的神经保护作用[J].医药导报,2012,31(9):1109-1110.
[13]郑海平,欧超,曹骥,等.仙人掌多糖主要组分成分分析及其对体外培养免疫细胞的影响[J].时珍国医国药,2012,23(7):1653-1656.
[14]虞旦,谢娅霏,苏宁,等.新鲜仙人掌多糖的提取及其抗氧化活性研究[J].食品研究与开发,2017,38(19):29-35.
[15]郭利平.仙人掌多糖的分离纯化、结构和生物活性研究进展[J].中国食品科添加剂(专论综述),2010:185-189.
[16]马世宏,单承莺,陈兴芬,等.仙人掌提取物在化妆品中的应用[J].日用化学品科学,2009,32(12):29-33.
[17]巴媛媛,王莹,朴美子.苯酚-硫酸法测定瓦尼木层孔菌菌丝体多糖含量的条件优化[J].食品工业科技,2011,32(5):389-391.
[18]蒋艳玲,赵明,梁功平,等.1,25-二羟维生素D3对Hacat细胞细胞增殖活性及基因组DNA和增殖相关基因启动子甲基化水平的影响[J].中华皮肤科杂志,2013,46(12):885-888.
[19]杨井,陶娟,李延,等.缺氧对Hacat细胞细胞HIF-1α,GLUT-1表达的影响及与细胞增殖的关系[J].中国皮肤性病学杂志,2009,23(10):621-623.
[20]白雨鑫,郭斌,韩冠英,等.萝藦果壳多糖提取工艺优化及其抗氧化活性研究[J].食品工业科技,2015,36(20);278-283.
[21]熊素玉,姚新奎,谭小海,等.不同温度及p H条件对乳酸菌生长影响的研究[J].新疆农业科学,2006,43(6):533-538.
[22]虞旦,谢娅霏,苏宁,等.新鲜仙人掌多糖的提取及其抗氧化活性研究[J].食品研究与开发,2017,38(19):29-34.
[23]Tcherakian C,Rivaud E,Catherinot E,et al.Pulmonary arterial hypertension related to HIV:is inflammation related to IL-6 the cornerstone?[J].Revue de Pneumologie Clinique,2011,67(4):250-257.
[24]杨敏,黄凤楼,傅海涛,等.原发性胆汁性肝硬化患者外周血IL-8及其受体CXCR1、CXCR2的表达及临床意义[J].临床实验诊断研究,2014:362-363.