氧化再生纤维素止血产品的体外降解研究
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摘要
目的研究氧化再生纤维素止血产品的体外降解。方法采用傅立叶红外光谱仪、电子扫描显微镜观察氧化再生纤维素止血产品降解前后的形态;分别采用凝胶色谱法和液相色谱与蒸发光散射检测器联用方法测定其降解产物。结果速即纱在降解初形态发生巨大变化;GPC确定其降解终点为10 d;确定其降解产物中含有葡萄糖(0.13%)与纤维二糖(0.17%)等成分;结论建立了GPC确定降解终点的方法,为降解终点的研究提供了新的思路和借鉴。
Objective To study the in vitro degradation of oxidized regenerated cellulose absorbable hemostatic products. Methods The morphology of the oxidized regenerated cellulose hemostatic products before and after degradation was observed by FTIR and SEM. The degradation products were determined by GPC and HILIC-ELSD. Results In the initial stage of degradation, there was a great change in morphology. GPC determined its degradation end point was 10 d; it was determined that its degradation products contained glucose(0.13%) and cellobiose(0.17%) and other components. Conclusion A method was established for determining the end point of degradation of oxidized regenerated cellulose, which provided a new idea and reference for the study of the degradation end point.
Objective To study the in vitro degradation of oxidized regenerated cellulose absorbable hemostatic products. Methods The morphology of the oxidized regenerated cellulose hemostatic products before and after degradation was observed by FTIR and SEM. The degradation products were determined by GPC and HILIC-ELSD. Results In the initial stage of degradation, there was a great change in morphology. GPC determined its degradation end point was 10 d; it was determined that its degradation products contained glucose(0.13%) and cellobiose(0.17%) and other components. Conclusion A method was established for determining the end point of degradation of oxidized regenerated cellulose, which provided a new idea and reference for the study of the degradation end point.
引文
[1]陈永忻,陈秀娟.生物可降解高分子材料在医学领域的应用[J].中国疗养医学,2003,12(6):37-40.
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[3]郑玉峰,刘彬,顾雪楠.可生物降解性医用金属材料的研究进展[J].材料导报,2009,23(1):1-6.
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[5]RASSU P C.Observed outcomes on the use of oxidized and regenerated cellulose polymer for breast conserving surgery-a case series[J].Ann Med Surg,2016,(5):57-66.
[6]NOGUEIRA C C,Ferreira S,Oliveira A,et al.Bronchoscopic hemostatic tamponade with oxidized regenerated cellulose for major hemoptysis control:two case reports[J].Revista Portuguesa de Pneumologia(English Edition),2010,16(6):917-920.
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[8]VIVEKANANDA B,VIKAS G,REENA B,et al.Modeling and experiments on release of metofluthrin from a thin cellulosicpolymer film[J].Chem Eng Res Design,2017,118:31-40.
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[15]胡文冉,范玲,李晓荣,等.棉花纤维中木质素的相对分子量[J].作物学报,2017,43(6):940-944.
[16]陈莉.凝胶渗透色谱测定聚电解质分子量及分子量分布的研究[D].武汉:武汉理工大学,2010.
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[19]劳万里,李改云,周群,等.红外光谱法测定杉木/聚丙烯复合材料中木粉和塑料含量[J].光谱学与光谱分析,2015,(6):1546-1550.
[20]刘刚,邢达,杨海珉,等.人体胆结石难溶物的扫描电镜观察和红外光谱研究[J].光谱学与光谱分析,2002,22(4):591-595.
[21]KARLSSON G,WINGE S,SANDBERG H.Separation of monosaccharides by hydrophilic interaction chromatography with evaporative light scattering detection[J].J Chromatogr A,2005,1092(2):246-249.
[22]中华人民共和国质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 16886.13-2001医疗器械生物学评价第13部分:聚合物医疗器械的降解产物的定性与定量[S].
[23]孔梅梅.利用PHA类生物降解新材料构建小口径人工血管的研究[D].天津:南开大学,2011.
[24]胡瑞.酶解法制备特定重均分子量及窄分布壳寡糖的工艺研究[D].武汉:武汉理工大学,2012.
[25]林慧,冯云,颜春荣,等.糖类化合物的分析方法研究进展[J].食品与药品,2014,16(6):448-452.
[26]CONDEZO-HOYOS L,P REZ-LOPEZ E,RUPEREZ P.Improved evaporative light scattering detection for carbohydrate analysis[J].Food Chem,2015,180:265-271.
[27]韩华欣,杨芳,肖丹,等.磷酸降解棉纤维制备低聚合度葡聚糖的研究[J].河北工业大学学报,2015,44(2):62-67.
[2]付东伟,闫玉华.生物可降解医用材料的研究进展[J].生物骨科材料与临床研究,2005,2(2):39-42.
[3]郑玉峰,刘彬,顾雪楠.可生物降解性医用金属材料的研究进展[J].材料导报,2009,23(1):1-6.
[4]HIROSHI Y,TETSUYA O,HIROYUKI T,et al.Applicaiotn of the solid dispersion method to the controlled release of medicine.VI.release mechanism of a slightly water soluble medicine and interaction between flurbiprofen and hydroxypropyl cellulose in solid dispersion[J].Chem Pharm Bull,1994,42(2):354-358.
[5]RASSU P C.Observed outcomes on the use of oxidized and regenerated cellulose polymer for breast conserving surgery-a case series[J].Ann Med Surg,2016,(5):57-66.
[6]NOGUEIRA C C,Ferreira S,Oliveira A,et al.Bronchoscopic hemostatic tamponade with oxidized regenerated cellulose for major hemoptysis control:two case reports[J].Revista Portuguesa de Pneumologia(English Edition),2010,16(6):917-920.
[7]KUCINSKA-LIPKA J,GUBANSKA I,JANIK H.Bacterial cellulose in the field of wound healing and regenerative medicine of skin:recent trends and future prospectives[J].Polymer Bull,2015,72(9):2399-2419.
[8]VIVEKANANDA B,VIKAS G,REENA B,et al.Modeling and experiments on release of metofluthrin from a thin cellulosicpolymer film[J].Chem Eng Res Design,2017,118:31-40.
[9]梅昕,马凤森,喻炎,等.高分子可降解生物材料的降解研究进展[J].材料导报,2016(S1):298-303.
[10]翟美玉,彭茜.生物可降解高分子材料[J].化学与粘合,2008(5):66-69.
[11]COSERI S,BILIUTA G,SIMIONESCU B C,et al.Oxidized cellulose-Survey of the most recent achievements[J].Carbohydrate polymers,2013,93(1):207-215.
[12]CHIA P L,FOO D.Use of oxidized regenerated cellulose to prevent pocket hematomas after cardiac electronic device implantation in patients on anticoagulants or dual antiplatelet therapy[J].Int JCardiol,2013,4(168):4406-4407.
[13]李玉基,史玉清.MAUDE数据库中速即纱不良事件报告分析[J].中国药物警戒,2013,10(5):312-314.
[14]李劲松.Surgicel致脑肿胀13例临床分析[J].江苏医药,2004,30(11):867-867.
[15]胡文冉,范玲,李晓荣,等.棉花纤维中木质素的相对分子量[J].作物学报,2017,43(6):940-944.
[16]陈莉.凝胶渗透色谱测定聚电解质分子量及分子量分布的研究[D].武汉:武汉理工大学,2010.
[17]林媛媛.氧化再生纤维素钠盐可吸收止血纱的降解机制研究[D].哈尔滨:哈尔滨工业大学,2013.
[18]梅昕,马凤森,喻炎,等.纤维素类可吸收止血产品体外降解终点判断的研究[J].中国医疗器械杂志,2016,40(2):125-127.
[19]劳万里,李改云,周群,等.红外光谱法测定杉木/聚丙烯复合材料中木粉和塑料含量[J].光谱学与光谱分析,2015,(6):1546-1550.
[20]刘刚,邢达,杨海珉,等.人体胆结石难溶物的扫描电镜观察和红外光谱研究[J].光谱学与光谱分析,2002,22(4):591-595.
[21]KARLSSON G,WINGE S,SANDBERG H.Separation of monosaccharides by hydrophilic interaction chromatography with evaporative light scattering detection[J].J Chromatogr A,2005,1092(2):246-249.
[22]中华人民共和国质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 16886.13-2001医疗器械生物学评价第13部分:聚合物医疗器械的降解产物的定性与定量[S].
[23]孔梅梅.利用PHA类生物降解新材料构建小口径人工血管的研究[D].天津:南开大学,2011.
[24]胡瑞.酶解法制备特定重均分子量及窄分布壳寡糖的工艺研究[D].武汉:武汉理工大学,2012.
[25]林慧,冯云,颜春荣,等.糖类化合物的分析方法研究进展[J].食品与药品,2014,16(6):448-452.
[26]CONDEZO-HOYOS L,P REZ-LOPEZ E,RUPEREZ P.Improved evaporative light scattering detection for carbohydrate analysis[J].Food Chem,2015,180:265-271.
[27]韩华欣,杨芳,肖丹,等.磷酸降解棉纤维制备低聚合度葡聚糖的研究[J].河北工业大学学报,2015,44(2):62-67.