超高效液相色谱-四极杆/飞行时间质谱筛查水果罐头中37种食品添加剂
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摘要
建立了超高效液相色谱-四极杆飞行时间质谱筛查水果罐头中37种食品添加剂的方法。试样用乙腈-水提取,以乙腈-乙酸铵溶液为流动相,在Acquity UPLC BEH C_(18)色谱柱上进行分离,电喷雾电离源负离子模式下采用全信息串联质谱信息采集的模式检测,通过各化合物的分子离子和特征碎片离子结合保留时间定性,外标法定量。结果表明,方法定量限为0.02~0.5 mg/kg,在0.5~50μg/m L内线性关系良好(r≥0.99),所有添加剂的回收率为79.5%~103.5%。该方法快速、灵敏、准确,适用于罐头中37种食品添加剂的快速筛查。
A screening method of 37 food additives in fruit cans was developed by ultra performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry.The samples were extracted with acetonitrilewater,and separated on an Acquity UPLC BEH C_(18) column using ammonium acetate solution-acetonitrile as the mobile phase and measured by UPLC/Q/TOF mass with electrospray ioninzation in negative mode.The qualitative analyses were based on the characteristic fragment ions of every compounds.The quantitation limitation were 0.02-0.5 mg/kg; the linear relationship was good in the concentration range of 0.5-50 μg/m L,the recoveries were 79.5%-103.5%.The method is simple,sensitive,accurate and suitable for screening food additives in fruit cans.
A screening method of 37 food additives in fruit cans was developed by ultra performance liquid chromatography coupled with quadrupole-time of flight-mass spectrometry.The samples were extracted with acetonitrilewater,and separated on an Acquity UPLC BEH C_(18) column using ammonium acetate solution-acetonitrile as the mobile phase and measured by UPLC/Q/TOF mass with electrospray ioninzation in negative mode.The qualitative analyses were based on the characteristic fragment ions of every compounds.The quantitation limitation were 0.02-0.5 mg/kg; the linear relationship was good in the concentration range of 0.5-50 μg/m L,the recoveries were 79.5%-103.5%.The method is simple,sensitive,accurate and suitable for screening food additives in fruit cans.
引文
[1]肖学成.薄层色谱法同时测定食品中4种添加剂的方法介绍[J].实用预防医学,1998,5(2):60-61.
[2]刘胜男,张国庆,王素方,等.高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂[J].轻工学报,2016,31(4):23-29.
[3]丁梅,辛若竹,卜丽伟.可变波长液相色谱法同时测定食品中18种常用食品添加剂研究[J].粮食与油脂,2014,27(6):50-55.
[4]林海丹,邹志飞,秦燕,等.高效液相色谱法同时测定食品中18种食品添加剂[J].食品科学,2013,34(8):228-231.
[5]马康,蒋孝雄,赵敏,等.高效液相色谱法同时测定软饮料中20种食品添加剂[J].分析化学,2012,40(11):1 661-1 667.
[6]黄永辉,余清,林钦,等.超高效液相色谱法同时测定碳酸饮料中的22种食品添加剂[J].分析测试学报,2011,30(8):877-882.
[7]刘英,尹州,邹晓筱,等.高效液相色谱法同时测定蜜饯中的16种食品添加剂[J].分析测试学报,2011,30(6):651-655.
[8]李颖.高效液相色谱法测定调味品和饮料中常见17种食品添加剂[J].中国卫生检验杂志,2010,20(11):2 738-2 740.
[9]王骏,胡梅,张卉,等.超高效液相色谱快速测定饮料中的16种食品添加剂[J].食品科学,2010,31(2):195-198.
[10]李秀琴,张庆合,杨总.高效液相色谱法同时测定肉制品中的6种食品添加剂[J].色谱,2010,28(12):1 204-1 208.
[11]李春娟,鞠福龙,李东刚.GC-MS法同时检测葡萄酒中10种防腐剂和抗氧化剂[J].中国酿造,2009,28(6):149-152.
[12]湛嘉,俞雪钧,黄伟,等.复杂基质食品中7种添加剂的气相色谱/质谱检测方法[J].分析科学学报,2008,24(6):729-731.
[13]曹淑瑞,徐芬,张雷,等.凝胶渗透色谱-气相色谱-串联质谱法同时测定食品中3种抗氧化剂[J].食品科学,2013,34(12):160-164.
[14]寇立娟,梁春实,臧金海.中性氧化铝柱净化-气相色谱/质谱法测定食品中富马酸二甲酯的含量[J].食品与发酵工业,2009,35(12):134-136.
[15]李东刚,李春娟,刘敬东.GC-MS-SIM检测糕点中的防腐剂和抗氧化剂[J].分析仪器,2009,40(4):56-60.
[16]王震,石丽珠.超高效液相色谱-串联质谱测定饮料和酒中防腐剂与甜味剂[J].食品安全质量检测学报,2014,5(4):1 179-1 184.
[17]于洋,卢静.离子色谱和液相色谱串联质谱法测定食品中的添加剂[J].化工管理,2015,28(14):66.
[18]李晶,徐济仓,缪明明,等.固相萃取-超高效液相色谱/串联质谱同时检测饮料中13种禁限用食品添加剂[J].分析科学学报,2013,29(4):488-492.
[19]曹娅,孙利,凌云,等.液相色谱-串联质谱测定食用植物油和调味油中27种添加剂(物)[J].食品科学,2013,34(4):129-134.
[20]冯峰,杨烁,凌云,等.超高效液相色谱-串联质谱快速筛查葡萄酒中的14种禁用食品添加剂[J].分析化学,2011,39(11):1 732-1 737.
[21]赵冰,沈学静.飞行时间质谱分析技术的发展[J].现代科学仪器,2006,23(4):30-33.
[22]吴炜亮,李晓明,朱文亮,等.飞行时间质谱技术及其在食品安全检测中的应用[J].食品与机械,2015,31(3):236-241.
[23]钱疆,杨方,陈弛,等.超高效液相色谱飞行时间质谱测定食品中36种合成色素[J].食品科学,2013,34(6):215-218.
[24]Commission Decision Implementing Council Directive 96/23/EC Concerning the Performance of Analytical Methods and the Interpretation of Results Text with EEA Relevance;Notified under Document Number C(2002)3044).
[2]刘胜男,张国庆,王素方,等.高效液相色谱法同时测定果蔬汁饮料中20种禁限用食品添加剂[J].轻工学报,2016,31(4):23-29.
[3]丁梅,辛若竹,卜丽伟.可变波长液相色谱法同时测定食品中18种常用食品添加剂研究[J].粮食与油脂,2014,27(6):50-55.
[4]林海丹,邹志飞,秦燕,等.高效液相色谱法同时测定食品中18种食品添加剂[J].食品科学,2013,34(8):228-231.
[5]马康,蒋孝雄,赵敏,等.高效液相色谱法同时测定软饮料中20种食品添加剂[J].分析化学,2012,40(11):1 661-1 667.
[6]黄永辉,余清,林钦,等.超高效液相色谱法同时测定碳酸饮料中的22种食品添加剂[J].分析测试学报,2011,30(8):877-882.
[7]刘英,尹州,邹晓筱,等.高效液相色谱法同时测定蜜饯中的16种食品添加剂[J].分析测试学报,2011,30(6):651-655.
[8]李颖.高效液相色谱法测定调味品和饮料中常见17种食品添加剂[J].中国卫生检验杂志,2010,20(11):2 738-2 740.
[9]王骏,胡梅,张卉,等.超高效液相色谱快速测定饮料中的16种食品添加剂[J].食品科学,2010,31(2):195-198.
[10]李秀琴,张庆合,杨总.高效液相色谱法同时测定肉制品中的6种食品添加剂[J].色谱,2010,28(12):1 204-1 208.
[11]李春娟,鞠福龙,李东刚.GC-MS法同时检测葡萄酒中10种防腐剂和抗氧化剂[J].中国酿造,2009,28(6):149-152.
[12]湛嘉,俞雪钧,黄伟,等.复杂基质食品中7种添加剂的气相色谱/质谱检测方法[J].分析科学学报,2008,24(6):729-731.
[13]曹淑瑞,徐芬,张雷,等.凝胶渗透色谱-气相色谱-串联质谱法同时测定食品中3种抗氧化剂[J].食品科学,2013,34(12):160-164.
[14]寇立娟,梁春实,臧金海.中性氧化铝柱净化-气相色谱/质谱法测定食品中富马酸二甲酯的含量[J].食品与发酵工业,2009,35(12):134-136.
[15]李东刚,李春娟,刘敬东.GC-MS-SIM检测糕点中的防腐剂和抗氧化剂[J].分析仪器,2009,40(4):56-60.
[16]王震,石丽珠.超高效液相色谱-串联质谱测定饮料和酒中防腐剂与甜味剂[J].食品安全质量检测学报,2014,5(4):1 179-1 184.
[17]于洋,卢静.离子色谱和液相色谱串联质谱法测定食品中的添加剂[J].化工管理,2015,28(14):66.
[18]李晶,徐济仓,缪明明,等.固相萃取-超高效液相色谱/串联质谱同时检测饮料中13种禁限用食品添加剂[J].分析科学学报,2013,29(4):488-492.
[19]曹娅,孙利,凌云,等.液相色谱-串联质谱测定食用植物油和调味油中27种添加剂(物)[J].食品科学,2013,34(4):129-134.
[20]冯峰,杨烁,凌云,等.超高效液相色谱-串联质谱快速筛查葡萄酒中的14种禁用食品添加剂[J].分析化学,2011,39(11):1 732-1 737.
[21]赵冰,沈学静.飞行时间质谱分析技术的发展[J].现代科学仪器,2006,23(4):30-33.
[22]吴炜亮,李晓明,朱文亮,等.飞行时间质谱技术及其在食品安全检测中的应用[J].食品与机械,2015,31(3):236-241.
[23]钱疆,杨方,陈弛,等.超高效液相色谱飞行时间质谱测定食品中36种合成色素[J].食品科学,2013,34(6):215-218.
[24]Commission Decision Implementing Council Directive 96/23/EC Concerning the Performance of Analytical Methods and the Interpretation of Results Text with EEA Relevance;Notified under Document Number C(2002)3044).