基于UHPLC-Q-TOF-MS/MS的白芍炒制前后化学成分研究
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摘要
目的:采用超高效液相色谱串联四极杆飞行时间质谱法,探讨白芍炒制前后化学成分的变化。方法:采用AgilentC18色谱柱,0. 1%甲酸水溶液-乙腈梯度洗脱,体积流量0. 3 m L/min,串联四级杆飞行时间质谱分别测定不同产地各10批生、炒白芍中的化学成分。结果:分别在生、炒白芍中鉴定出40种化学成分。结论:通过UHPLC-Q-TOF-MS/MS技术,为分析生、炒白芍中的化学成分提供一种快速、高效的分析方法,为综合评价生白芍和炒白芍的质量提供参考。
Objective: By using ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry,to investigate the changes of chemical composition in Paeonia lactiflora before and after progressed. Methods: Agilent C18 column,0. 1% formic acid aqueous solution-acetonitrile gradient elution,volume flow rate 0. 3 mL/min,tandem four-stage rod time-of-flight mass spectrometry were used to determine the chemical composition of 10 batches of raw and fried white peony in different producing areas. Results: A total of 40 chemical components were identified in raw Paeonia lactiflora( RPL) and processed Paeonia lactiflora( PPL). Conclusion: UHPLC-Q-TOF-MS/MS technology provides a fast and efficient analytical method for the analysis of chemical constituents in RPL and PPL,which provides a reference for comprehensive evaluation of the quality of RPL and PPL.
Objective: By using ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry,to investigate the changes of chemical composition in Paeonia lactiflora before and after progressed. Methods: Agilent C18 column,0. 1% formic acid aqueous solution-acetonitrile gradient elution,volume flow rate 0. 3 mL/min,tandem four-stage rod time-of-flight mass spectrometry were used to determine the chemical composition of 10 batches of raw and fried white peony in different producing areas. Results: A total of 40 chemical components were identified in raw Paeonia lactiflora( RPL) and processed Paeonia lactiflora( PPL). Conclusion: UHPLC-Q-TOF-MS/MS technology provides a fast and efficient analytical method for the analysis of chemical constituents in RPL and PPL,which provides a reference for comprehensive evaluation of the quality of RPL and PPL.
引文
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[14]翁金月,曹岗,吴鑫,等.基于多指标成分的白芍麸炒前后特征指纹图谱比较研究[J].中华中医药学刊,2015,33(5):1056-1057.
[15]梁德勤,赵园园,罗云,等.不同加工方式亳白芍的高效液相指纹图谱及多成分含量比较[J].安徽中医药大学学报,2018,37(2):85-89.
[16]周海玲,许舜军,周若龙,等.白芍、赤芍化学成分的高效液相色谱-飞行时间串联质谱分析[J].中药材,2018,41(7):1638-1641.
[17]练杭芸,徐王彦君,梁乾德,等.UPLC-QTOF MS法比较赤芍、白芍煎液化学成分差异[J].质谱学报,2014,35(3):269-278.
[18]Minxia Z,Zhe C,Pei L,et al.Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS[J].China Journal of Chinese Materia Medica,2011,36(12):1641-1643.
[2]贺妮,侯宇,柏慧,等.白芍提取物抗抑郁及抗炎作用的研究[J].世界中西医结合杂志,2018,13(3):348-352.
[3]段文娟,李月,杨国红,等.白芍对斑马鱼促血管生成和抗血栓作用的研究[J].时珍国医国药,2018,29(4):834-837.
[4]左志燕,詹淑玉,黄嬛,等.白芍总苷保肝作用的药动学和药效学研究进展[J].中国中药杂志,2017,41(20):22-27.
[5]蔡宝昌.中药炮制学[M].北京:中国中医药出版社,2012:167.
[6]谭淑瑜,肖雪,朴胜华,等.白芍水溶性成分快速鉴定研究[J].中药材,2017,40(1):94-100.
[7]Cao W J,Zhang W,Liu J J.Paeoniflorin improves survival in LPS-challenged mice through the suppression of TNF-alpha and IL-1 beta release and augmentation of IL-10 production[J].International Immunopharmacology,2010,11(2):172-178.
[8]Wang Q S,Gao T,Cui Y L,et al.Comparative studies of paeoniflorin and albiflorin from Paeonia lactiflora on anti-inflammatory activities[J].Pharmaceutical Biology,2014,52(9):1189-1195.
[9]Yen,Pham Hai,et al.A new monoterpene glycoside from the roots ofPaeonia lacti-flora increases the differentiation of osteoblastic MC3T3-E1 cell[J].Archives of Pharmacal Research,2007,30(10):1179-1185.
[10]陶益.地黄炮制前后化学成分的UHPLC-Q-TOF/MS比较研究[J].中药新药与临床药理,2016,27(1):102-106.
[11]陈坚平,钟艳梅,余楚钦,等.UHPLC-Q-TOF-MS法分析蒿甲虚热清颗粒的化学成分[J].中药材,2017,40(6):1368-1372.
[12]王志成,季雪,崔韶婧,等.基于超高效液相色谱-四级杆飞行时间质谱技术的通脉颗粒质量评价研究[J].药物评价研究,2016,39(4):603-607.
[13]童黄锦,白发平,汪小莉,等.不同产地白芍药材的指纹图谱研究[J].中医学报,2014,29(9):1326-1329.
[14]翁金月,曹岗,吴鑫,等.基于多指标成分的白芍麸炒前后特征指纹图谱比较研究[J].中华中医药学刊,2015,33(5):1056-1057.
[15]梁德勤,赵园园,罗云,等.不同加工方式亳白芍的高效液相指纹图谱及多成分含量比较[J].安徽中医药大学学报,2018,37(2):85-89.
[16]周海玲,许舜军,周若龙,等.白芍、赤芍化学成分的高效液相色谱-飞行时间串联质谱分析[J].中药材,2018,41(7):1638-1641.
[17]练杭芸,徐王彦君,梁乾德,等.UPLC-QTOF MS法比较赤芍、白芍煎液化学成分差异[J].质谱学报,2014,35(3):269-278.
[18]Minxia Z,Zhe C,Pei L,et al.Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS[J].China Journal of Chinese Materia Medica,2011,36(12):1641-1643.