不同低氮胁迫对大麦地方品种苗期耐低氮性的影响
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摘要
为进一步挖掘大麦(Hordeum vulgare)地方品种耐低氮性潜能,从前期研究0.50 mmol·L~(-1) NH_4NO_3低氮胁迫筛选到的5份耐低氮大麦地方品种中随机挑选了3个,通过两个氮素浓度更低的低氮胁迫(分别为0.24和0.10mmol·L~(-1) NH_4NO_3)对其进行耐低氮鉴定。结果发现,大麦地方品种‘上海有芒大麦’在这两个低氮胁迫下都表现出较好的耐低氮性,‘六柱头紫大麦’仅在0.24 mmol·L~(-1) NH_4NO_3下耐低氮性较好,而‘宝山老脱须’在这两个低氮胁迫下耐低氮性都比较差。变异系数分析进一步验证了干重性状比较适合作为大麦耐低氮筛选指标,而株高不太适合。本研究又进一步探索了氮素相关生理性状作为大麦耐低氮评价的可行性,并且讨论了在低氮胁迫下氮素吸收和利用相关性状对大麦耐低氮的重要性。
To further explore the potential of low nitrogen tolerance of barley(Hordeum vulgare)landraces,three of the five barley landraces with low nitrogen tolerance under 0.50 mmol·L~(-1) NH_4NO_3 treatment were randomly selected for identification of low nitrogen tolerance under two lower nitrogen stresses(0.24 and 0.10mmol·L~(-1) NH_4NO_3).The results show that the barley landrace of‘Shanghai Youmang Damai’was tolerant to both of the low nitrogen stresses,while‘Liuzhutou Zidamai’was only tolerant to low nitrogen stress of 0.24mmol·L~(-1) NH_4NO_3,and ‘Boshan Laotuoxu’ was not tolerant to the two lower nitrogen stresses.Coefficient of variation analysis suggests that dry weight related traits were more suitable as screening indices for low nitrogen tolerance,while the trait of plant height was not.In addition,this study further explores the possibility of low nitrogen tolerance identification by physiological traits related nitrogen uptake or utilization,and discusses the importance of nitrogen uptake or utilization on low nitrogen tolerance.
To further explore the potential of low nitrogen tolerance of barley(Hordeum vulgare)landraces,three of the five barley landraces with low nitrogen tolerance under 0.50 mmol·L~(-1) NH_4NO_3 treatment were randomly selected for identification of low nitrogen tolerance under two lower nitrogen stresses(0.24 and 0.10mmol·L~(-1) NH_4NO_3).The results show that the barley landrace of‘Shanghai Youmang Damai’was tolerant to both of the low nitrogen stresses,while‘Liuzhutou Zidamai’was only tolerant to low nitrogen stress of 0.24mmol·L~(-1) NH_4NO_3,and ‘Boshan Laotuoxu’ was not tolerant to the two lower nitrogen stresses.Coefficient of variation analysis suggests that dry weight related traits were more suitable as screening indices for low nitrogen tolerance,while the trait of plant height was not.In addition,this study further explores the possibility of low nitrogen tolerance identification by physiological traits related nitrogen uptake or utilization,and discusses the importance of nitrogen uptake or utilization on low nitrogen tolerance.
引文
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Ma XJ,Zhang XT,Huang YF,et al(2017).Establishment and application of critical nitrogen dilution curve for wheat based on leaf dry matter.Plant Physiol J,53(7):1313-1321(in Chinese with English abstract)[马晓晶,张小涛,黄玉芳等(2017).小麦叶片临界氮浓度稀释曲线的建立与应用.植物生理学报,53(7):1313-1321]
Piao ZZ,Han LZ,Koh HJ(2003).Variations of nitrogen use efficiency by rice genotype.Chin J Rice Sci,17(3):233-238(in Chinese with English abstract)[朴钟泽,韩龙植,高熙宗(2003).水稻不同基因型氮素利用效率差异.中国水稻科学,17(3):233-238]
Qiang X(2015).The varieties screening of nitrogen use efficiency in Hordeum vulgare and bioinformatics analysis of GS gene(dissertation).Lanzhou:Gansu Agricultural University(in Chinese with English abstract)[强欣(2015).大麦不同氮利用效率品种筛选及GS基因的生物信息学分析(学位论文).兰州:甘肃农业大学]
Quan X,Zeng J,Ye L,et al(2016).Transcriptome profiling analysis for two Tibetan wild barley genotypes in responses to low nitrogen.BMC Plant Biol,16:30
Shen J,Li Y,Liu X,et al(2013).Atmospheric dry and wet nitrogen deposition on three contrasting land use types of an agricultural catchment in subtropical central China.Atmos Environ,67(2):415-424
Tong H,Yu X,Mei H,et al(2007).Screening of nitrogen nutrition efficient genotypes in rice seedlings.J Zhejiang Agr Sci,(5):537-541(in Chinese)[童汉华,余新桥,梅捍卫等(2007).水稻苗期氮素营养高效基因型的筛选.浙江农业科学,(5):537-541]
Wu JN,Wei XD,Li X,et al(2018).Research progress in nitrogen use efficiency in plants.Plant Physiol J,54(9):1401-1408(in Chinese with English abstract)[武姣娜,魏晓东,李霞等(2018).植物氮素利用效率的研究进展.植物生理学报,54(9):1401-1408]
Yang LN,Hu H,Zhu B,et al(2014).Genotypic variations of N use efficiency in Tibetan wild and cultivated barleys.JZhejiang Univ-Agr Life Sci,40(2):155-164
Zhang F,Cui Z,Fan M,et al(2011).Integrated soil-crop system management:reducing environmental risk while increasing crop productivity and improving nutrient use efficiency in China.J Environ Qual,40(4):1051-1057
Zhong S,Chen R,Tao Y,et al(2017).Study on the difference of nitrogen uptake and utilization efficiency of different tobacco genotypes.Chin Tob Sci,38(4):58-63(in Chinese with English abstract)[钟思荣,陈仁霄,陶瑶等(2017).不同烟草基因型氮素吸收效率与利用效率差异.中国烟草科学,38(4):58-63]
Cao G,Liang MZ(2002).Nitrogen:one of the essential macroelements for plants in balanced cultivation system.Soils Fert,(4):48-50(in Chinese)[曹恭,梁鸣早(2002).氮--平衡栽培体系中植物必需的大量元素.土壤肥料,(4):48-50]
Cassman KG,Dobermann A,Walters DT,et al(2003).Meeting cereal demand while protecting natural resources and improving environmental quality.Annu Rev Environ Resour,28:315-358
Chen ZW,He T,Lu RJ,et al(2010).Biological responses of different-genotype barley to low nitrogen stress at seedling stage.Acta Agr Shanghai,26(1):28-32(in Chinese with English abstract)[陈志伟,何婷,陆瑞菊等(2010).不同基因型大麦苗期对低氮胁迫的生物学响应.上海农业学报,26(1):28-32]
Cheng JF,Dai TB,Cao WX,et al(2005).Classification,identification and evaluation of nitrogen nutrition efficiencies in rice germplasms at seedling stage.Acta Agron Sin,31(12):1640-1647(in Chinese with English abstract)[程建峰,戴廷波,曹卫星等(2005).稻种资源苗期氮素营养效率的分类、鉴定与评价.作物学报,31(12):1640-1647]
Fischer RA,Byerlee D,Edmeades GO(2009).Can technology deliver on the yield challenge to 2050?Rome:FAOExpert Meeting on How to Feed the World in 2050,https://ageconsearch.umn.edu/record/55481
Gao R,Guo G,Fang C,et al(2018).Rapid generation of barley mutant lines with high nitrogen uptake efficiency by microspore mutagenesis and field screening.Front Plant Sci,9:450
Good AG,Beatty PH(2011).Fertilizing nature:a tragedy of excess in the commons.PLoS Biol,9(8):e1001124
Jiang Q,Chen Z,Liu C,et al(2019).Screening and identification indices of low-nitrogen tolerance for barley landraces at seedling stage.Acta Agr Boreali-Sin,34(1):148-155(in Chinese with English abstract)[姜琪,陈志伟,刘成洪等(2019).大麦地方品种苗期耐低氮筛选和鉴定指标的研究.华北农学报,34(1):148-155]
Ladha JK,Tirol-Padre A,Reddy CK,et al(2016).Global nitrogen budgets in cereals:a 50-year assessment for maize,rice,and wheat production systems.Sci Rep,6:19355
Liu MN,Liu XX,Ding WY,et al(2012).Variation in nitrogen uptake and utilization efficiency in spinach genotypes and its evaluation.J Zhejiang Univ-Agr Life Sci,38(5):599-607(in Chinese with English abstract)[刘敏娜,刘晓霞,丁文雅等(2012).不同菠菜基因型氮素吸收与利用效率的差异及其评价.浙江大学学报(农业与生命科学版),38(5):599-607]
Liu P,Wu AL,Wang JS,et al(2018).Nitrogen use efficiency and physiological responses of different sorghum genotypes influenced by nitrogen deficiency.Sci Agr Sin,51(16):3074-3083(in Chinese with English abstract)[刘鹏,武爱莲,王劲松等(2018).不同基因型高粱的氮效率及对低氮胁迫的生理响应.中国农业科学,51(16):3074-3083]
Ma XJ,Zhang XT,Huang YF,et al(2017).Establishment and application of critical nitrogen dilution curve for wheat based on leaf dry matter.Plant Physiol J,53(7):1313-1321(in Chinese with English abstract)[马晓晶,张小涛,黄玉芳等(2017).小麦叶片临界氮浓度稀释曲线的建立与应用.植物生理学报,53(7):1313-1321]
Piao ZZ,Han LZ,Koh HJ(2003).Variations of nitrogen use efficiency by rice genotype.Chin J Rice Sci,17(3):233-238(in Chinese with English abstract)[朴钟泽,韩龙植,高熙宗(2003).水稻不同基因型氮素利用效率差异.中国水稻科学,17(3):233-238]
Qiang X(2015).The varieties screening of nitrogen use efficiency in Hordeum vulgare and bioinformatics analysis of GS gene(dissertation).Lanzhou:Gansu Agricultural University(in Chinese with English abstract)[强欣(2015).大麦不同氮利用效率品种筛选及GS基因的生物信息学分析(学位论文).兰州:甘肃农业大学]
Quan X,Zeng J,Ye L,et al(2016).Transcriptome profiling analysis for two Tibetan wild barley genotypes in responses to low nitrogen.BMC Plant Biol,16:30
Shen J,Li Y,Liu X,et al(2013).Atmospheric dry and wet nitrogen deposition on three contrasting land use types of an agricultural catchment in subtropical central China.Atmos Environ,67(2):415-424
Tong H,Yu X,Mei H,et al(2007).Screening of nitrogen nutrition efficient genotypes in rice seedlings.J Zhejiang Agr Sci,(5):537-541(in Chinese)[童汉华,余新桥,梅捍卫等(2007).水稻苗期氮素营养高效基因型的筛选.浙江农业科学,(5):537-541]
Wu JN,Wei XD,Li X,et al(2018).Research progress in nitrogen use efficiency in plants.Plant Physiol J,54(9):1401-1408(in Chinese with English abstract)[武姣娜,魏晓东,李霞等(2018).植物氮素利用效率的研究进展.植物生理学报,54(9):1401-1408]
Yang LN,Hu H,Zhu B,et al(2014).Genotypic variations of N use efficiency in Tibetan wild and cultivated barleys.JZhejiang Univ-Agr Life Sci,40(2):155-164
Zhang F,Cui Z,Fan M,et al(2011).Integrated soil-crop system management:reducing environmental risk while increasing crop productivity and improving nutrient use efficiency in China.J Environ Qual,40(4):1051-1057
Zhong S,Chen R,Tao Y,et al(2017).Study on the difference of nitrogen uptake and utilization efficiency of different tobacco genotypes.Chin Tob Sci,38(4):58-63(in Chinese with English abstract)[钟思荣,陈仁霄,陶瑶等(2017).不同烟草基因型氮素吸收效率与利用效率差异.中国烟草科学,38(4):58-63]