实验动物支气管鲍特杆菌检测能力验证结果与分析
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摘要
目的开展2017年能力验证活动,评价国内实验动物检测实验室对支气管鲍特杆菌的检测能力。方法依据CNAS相关文件,中国食品药品检定研究院作为能力验证提供者,制备含有支气管鲍特杆菌及产气巴斯德杆菌和大肠杆菌为干扰菌的比对样品,提供给参加实验室,要求在规定时限内反馈检测结果及报告。结果所制备三种样品的平均含菌量均大于1×10~8CFU/mL,37℃加速稳定性时间不少于20 d,符合比对要求。全国共有18个省市的25个实验动物检测实验室参加,均按时反馈结果和报告,满意率88%。结论本次能力验证为实验动物呼吸道细菌比对项目的进一步开展奠定了基础。大部分参加实验室具备一致可靠的支气管鲍特杆菌检测能力。
Objective To carry out 2017 proficiency testing(PT) activities, and evaluate the detection capability of the domestic quality testing institute of laboratory animals for Bordetella bronchiseptica. Method As the proficiency testing provider(PTP), National Institutes for Food and Drug Control(NIFDC)made animal respiratory tract samples with B.bronchiseptica, Pasteurella aerogenes and Escherichia colias confusing bacteria samples which were provided to the participating laboratories.The laboratories must be feed back result and reports within the prescribed time limit. Result The average amount of bacteria prepared in the three kinds of samples were more than 1×10~8CFU/mL, and the 37 ℃ acceleration stability was greater than 20 d.The prepared samples met the PT requirements.A total of 25 laboratories of 18 provinces participated in this PT that returned the result and report on time. Three laboratories result were not satisfactory. The overall satisfaction rate was 88%. Conclusion This PT laid the foundation for the further development of laboratory animal respiratory bacteria. Most of the participating laboratories had consistent and reliable detection capability to B.bronchiseptica.
Objective To carry out 2017 proficiency testing(PT) activities, and evaluate the detection capability of the domestic quality testing institute of laboratory animals for Bordetella bronchiseptica. Method As the proficiency testing provider(PTP), National Institutes for Food and Drug Control(NIFDC)made animal respiratory tract samples with B.bronchiseptica, Pasteurella aerogenes and Escherichia colias confusing bacteria samples which were provided to the participating laboratories.The laboratories must be feed back result and reports within the prescribed time limit. Result The average amount of bacteria prepared in the three kinds of samples were more than 1×10~8CFU/mL, and the 37 ℃ acceleration stability was greater than 20 d.The prepared samples met the PT requirements.A total of 25 laboratories of 18 provinces participated in this PT that returned the result and report on time. Three laboratories result were not satisfactory. The overall satisfaction rate was 88%. Conclusion This PT laid the foundation for the further development of laboratory animal respiratory bacteria. Most of the participating laboratories had consistent and reliable detection capability to B.bronchiseptica.
引文
[1] CNAS-RL02.能力验证规则[S].中国合格评定国家认可委员会.2016.
[2] 李晓波,王洪,付瑞,等.实验大鼠细小病毒H-1株抗体检测能力验证结果评价[J].中国药事,2014,28(9):990-994.
[3] 王吉,付瑞,李晓波等.实验小鼠呼肠孤病毒Ⅲ型抗体的实验室检测能力验证结果评价[J].中国实验动物学报,2016,24(2):183-187.
[4] 付瑞,王洪,王淑菁,等.兔出血症病毒抗体的实验室检测能力验证结果评价[J].中国实验动物学报,2016,24(2):188-190,198.
[5] 邢进,冯育芳,王洪,等.实验动物中绿脓杆菌检测能力验证的结果与分析[J].中国药事,2014,28(9):986-989.
[6] 冯育芳,邢进,付瑞,等.实验动物金黄色葡萄球菌的实验室检测能力验证结果评价[J].中国实验动物学报,2016,24(2):195-198.
[7] 邢进,冯育芳,王洪,等.实验动物中沙门菌的实验室检测能力验证的结果与分析[J].中国实验动物学报,2016,24(2):191-194.
[8] 王洪,魏杰,李芳芳,等.实验动物质检机构碱性磷酸酶-1测定能力验证评价[J].中国药事,2014,28(12):1339-1341.
[9] 魏杰,王洪,李芳芳,等.实验室能力验证用酯酶-3标准样品的均匀性和稳定性研究[J].中国药事,2014,28(9):990-994.
[10] CNAS-RL03.能力验证提供者认可准则[S].中国合格评定国家认可委员会.2010.
[11] 范薇,隋丽华,刘永梅,等.普通级啮齿类实验动物部分细菌携带情况的初步调查[J].实验动物科学与管理,2001,18(2):18-20.
[12] 田克恭,贺争鸣,刘群,等.实验动物疫病学[M].北京:中国农业出版社,2005.
[13] NY/T 546-2015.猪传染性萎缩性鼻炎诊断技术[S].2015.
[14] Brenner D J,Krieg N R,Staley J T,et al.Bergey’s manual of systematic bacteriology,Vol 2:The Proteobacteria,Part C:The Alpha-,Beta-,Delta-,and Epsilonproteobacteria[M].2nd ed.New York:Springer,2005:662-670.
[15] GB/T 14926.6-2001 实验动物支气管鲍特杆菌检测方法[S].北京:中国标准出版社,2001.
[16] Weisburg W G,Barns S M,Pelletier D A,et al.16S ribosomal DNA amplification for phylogenetic study[J].J Bacteriol.1991;173(2):697-703.
[17] CNAS-CL09.检测和校准实验室能力认可准则在微生物检测领域的应用说明[S].中国合格评定国家认可委员会.2014.
[18] Coutinho T A,Bernardi M L,de Itapema Cardoso M R,et al.Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection[J].Braz J Microbiol,2009,40(3):470-479.
[19] Canonne A M,Billen F,Tual C,et al.Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella bronchiseptica Infection[J].J Vet Intern Med.J Vet Intern Med,2016,30(4):1204-1209.
[20] Tizolova A,Brun D,Guiso N,et al.Development of real-time PCR assay for differential detection of Bordetella bronchiseptica and Bordetella parapertussis[J].DiagnMicrobiol Infect Dis,2014,78(4):347-351.
[21] Hozbor D,Fouque F,Guiso N,et al.Detection of Bordetella bronchiseptica by the polymerase chain reaction[J].Res Microbiol,1999,150(5):333-341.
[22] Simpson W,Simmons D J.Problems associated with the identification of bordetellabronchiseptica[J].Lab Anim,1976,10(1):47-48.
[2] 李晓波,王洪,付瑞,等.实验大鼠细小病毒H-1株抗体检测能力验证结果评价[J].中国药事,2014,28(9):990-994.
[3] 王吉,付瑞,李晓波等.实验小鼠呼肠孤病毒Ⅲ型抗体的实验室检测能力验证结果评价[J].中国实验动物学报,2016,24(2):183-187.
[4] 付瑞,王洪,王淑菁,等.兔出血症病毒抗体的实验室检测能力验证结果评价[J].中国实验动物学报,2016,24(2):188-190,198.
[5] 邢进,冯育芳,王洪,等.实验动物中绿脓杆菌检测能力验证的结果与分析[J].中国药事,2014,28(9):986-989.
[6] 冯育芳,邢进,付瑞,等.实验动物金黄色葡萄球菌的实验室检测能力验证结果评价[J].中国实验动物学报,2016,24(2):195-198.
[7] 邢进,冯育芳,王洪,等.实验动物中沙门菌的实验室检测能力验证的结果与分析[J].中国实验动物学报,2016,24(2):191-194.
[8] 王洪,魏杰,李芳芳,等.实验动物质检机构碱性磷酸酶-1测定能力验证评价[J].中国药事,2014,28(12):1339-1341.
[9] 魏杰,王洪,李芳芳,等.实验室能力验证用酯酶-3标准样品的均匀性和稳定性研究[J].中国药事,2014,28(9):990-994.
[10] CNAS-RL03.能力验证提供者认可准则[S].中国合格评定国家认可委员会.2010.
[11] 范薇,隋丽华,刘永梅,等.普通级啮齿类实验动物部分细菌携带情况的初步调查[J].实验动物科学与管理,2001,18(2):18-20.
[12] 田克恭,贺争鸣,刘群,等.实验动物疫病学[M].北京:中国农业出版社,2005.
[13] NY/T 546-2015.猪传染性萎缩性鼻炎诊断技术[S].2015.
[14] Brenner D J,Krieg N R,Staley J T,et al.Bergey’s manual of systematic bacteriology,Vol 2:The Proteobacteria,Part C:The Alpha-,Beta-,Delta-,and Epsilonproteobacteria[M].2nd ed.New York:Springer,2005:662-670.
[15] GB/T 14926.6-2001 实验动物支气管鲍特杆菌检测方法[S].北京:中国标准出版社,2001.
[16] Weisburg W G,Barns S M,Pelletier D A,et al.16S ribosomal DNA amplification for phylogenetic study[J].J Bacteriol.1991;173(2):697-703.
[17] CNAS-CL09.检测和校准实验室能力认可准则在微生物检测领域的应用说明[S].中国合格评定国家认可委员会.2014.
[18] Coutinho T A,Bernardi M L,de Itapema Cardoso M R,et al.Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection[J].Braz J Microbiol,2009,40(3):470-479.
[19] Canonne A M,Billen F,Tual C,et al.Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella bronchiseptica Infection[J].J Vet Intern Med.J Vet Intern Med,2016,30(4):1204-1209.
[20] Tizolova A,Brun D,Guiso N,et al.Development of real-time PCR assay for differential detection of Bordetella bronchiseptica and Bordetella parapertussis[J].DiagnMicrobiol Infect Dis,2014,78(4):347-351.
[21] Hozbor D,Fouque F,Guiso N,et al.Detection of Bordetella bronchiseptica by the polymerase chain reaction[J].Res Microbiol,1999,150(5):333-341.
[22] Simpson W,Simmons D J.Problems associated with the identification of bordetellabronchiseptica[J].Lab Anim,1976,10(1):47-48.