灰葡萄孢菌侵染对采后猕猴桃果实生理生化指标的影响
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摘要
为给采后猕猴桃果实的保鲜、病害防治及抗病育种提供参考依据,以红肉系列的当家品种‘红阳’猕猴桃为试材,以浓度为104个/mL的灰葡萄孢菌悬浮液对其果实分别进行了0、24、48、72、96、120 h的侵染处理,就不同处理对诱发猕猴桃果实采后病变腐烂及其生理生化指标的影响情况进行了试验研究。试验结果表明:各处理的猕猴桃果实内4种防御酶(POD、SOD、CAT和PAL)活性、总酚和木质素含量均呈先升高后下降的变化趋势;其内源激素IAA和GA含量均呈先升高后降低的变化趋势,SA含量则呈逐渐增长趋势,ABA含量呈先上升后降低再升高的变化趋势。分析结果表明:猕猴桃果实在受到灰葡萄孢菌侵染胁迫下能有效激活其与抗病相关的生理生化指标,通过形成较强的防御酶活性或提高其木质素、多酚及内源激素的含量来缓解病菌逆境对其造成的伤害;各项生理生化指标在猕猴桃果实与灰葡萄孢菌的互作过程中是相互联系的,而不是某一项生理生化指标(防御酶或激素)单独起作用的。
In order to provide some references for preservation, disease control and disease-resistance breeding of postharvest kiwifruit, using a red flesh cultivar of ‘Hongyang' kiwifruit as test materials, fruits were respectively infected with Botrytis cinerea suspension(104 spores/mL) for 0, 24, 48, 72, 96 and 120 h, and effects of different treatments on rot resistance and some physiological and biochemical indexes of kiwifruit were studied. The results showed that activities of four defense enzymes(POD, SOD, CAT and PAL), total phenol and lignin contentsin treated kiwifruits were all increased,and then decreased. Among the endogenous hormones, contents of IAA and GA were firstly increased and then decreased,while SA content was increased gradually, ABA content was increased, decreased, and finally increased. These indicate that kiwifruit could effectively activate its physiological and biochemical indexes related to disease resistance under stress of B. cinerea infecting, and damages caused bypathogens could be alleviated through forming stronger defense enzyme activity or increasing contents of lignin, polyphenols and endogenous hormones. Physiological and biochemical indexes were interrelated in interaction between kiwifruit and B. cinerea, and rather than acting result of single physiological and biochemical index(defense enzyme or hormone).
In order to provide some references for preservation, disease control and disease-resistance breeding of postharvest kiwifruit, using a red flesh cultivar of ‘Hongyang' kiwifruit as test materials, fruits were respectively infected with Botrytis cinerea suspension(104 spores/mL) for 0, 24, 48, 72, 96 and 120 h, and effects of different treatments on rot resistance and some physiological and biochemical indexes of kiwifruit were studied. The results showed that activities of four defense enzymes(POD, SOD, CAT and PAL), total phenol and lignin contentsin treated kiwifruits were all increased,and then decreased. Among the endogenous hormones, contents of IAA and GA were firstly increased and then decreased,while SA content was increased gradually, ABA content was increased, decreased, and finally increased. These indicate that kiwifruit could effectively activate its physiological and biochemical indexes related to disease resistance under stress of B. cinerea infecting, and damages caused bypathogens could be alleviated through forming stronger defense enzyme activity or increasing contents of lignin, polyphenols and endogenous hormones. Physiological and biochemical indexes were interrelated in interaction between kiwifruit and B. cinerea, and rather than acting result of single physiological and biochemical index(defense enzyme or hormone).
引文
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[13]李忠光,李江鸿,杜朝昆,等.在单一提取系统中同时测定五种植物抗氧化酶[J].云南师范大学学报,2002,22(6):44-48.
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[15]AEBI H.Catalase in vitro[J].Methods in Enzymology,1984,105:121-126.
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[21]HUANG Z G,KABIR M H,XIAO Y,et al.Principles and Practice of ABA Analysis[C]//Abscisic Acid:Metabolism,Transport and Signaling.New York and London:Springer Dordrecht Heidelberg,2014.
[22]LIU X,YANG Y L,LIN W H,et al.Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry[J].Chinese Science Bulletin,2010,55(21):2231-2235.
[23]张华峰,王志敏,韩娇,等.生姜离体形态发生过程中生理生化指标的变化[J].南方农业,2009,3(3):71-75.
[24]朱诗禹,崔娟,徐伟,等.精异丙甲草胺苗前封闭处理对大豆苗期生长及其生理生化指标的影响[J].植物保护学报,2016,43(4):677-682.
[25]GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48(12):909-930.
[26]王玲利,刘超,曾明,等.采后处理对“黄冠”梨膜脂过氧化和保护酶活性的影响[J].中国南方果树,2017,46(2):132-136.
[27]庞宏东,胡兴宜,胡文杰,等.淹水胁迫对枫杨等3个树种生理生化特性的影响[J].中南林业科技大学学报,2018,38(10):15-21.
[28]刘雪,彭冶,范俊俊,等.淹水胁迫对湖北海棠生长和生理特性的影响[J].经济林研究,2018,36(1):35-42.
[29]张青,彭祚登.七叶树幼苗对水分胁迫和复水的生理响应[J].中南林业科技大学学报,2018,38(4):46-53.
[30]李桦,梁春强,吕茳,等.草酸对冷藏‘华优’猕猴桃果实木质化及相关酶活性的影响[J].园艺学报,2017,44(6):1085-1093.
[31]畅涛,杨成德,薛莉,等.珠芽蓼内生菌ZA1对马铃薯的防病促生研究[J].草业学报,2015,24(12):83-91.
[32]韩庆典,胡晓君,黄坤艳,等.小麦白粉病菌对小麦幼苗MDA含量及防御酶活性的影响[J].分子植物育种,2016,14(10):2803-2811.
[33]韩建东,曹远银.POD和PAL在小麦抗秆锈病中的动态变化[J].江苏农业科学,2009(2):109-110.
[34]孙翠红,徐翠莲,赵铭钦,等.壳寡糖衍生物纳米银微粒诱导烟株产生TMV抗性[J].中国烟草学报,2015,21(6):59-64.
[35]卜冰武,邱德文,曾红梅,等.大丽轮枝菌蛋白激发子PevD1诱导棉花抗病性及作用机理[J].植物病理学报,2014,44(3):254-264.
[36]李伟,熊谨,陈晓阳.木质素代谢的生理意义及其遗传控制研究进展[J].西北植物学报,2003,23(4):675-681.
[37]吴颖静,季浩,唐灿明.大丽轮枝菌侵染拟南芥的过程及诱导的防御反应研究[J].核农学报,2018,32(4):681-689.
[38]BLANCO-ULATE B,VINCENTI E,POWELL A L T,et al.Tomato transcriptome and mutant analyses suggest a role for plant stress hormones in the interaction between fruit and Botrytis cinerea[J].Frontiers in Plant Science,2013,4:142.
[39]何建社,张利,刘千里,等.岷江干旱河谷区典型灌木对干旱胁迫的生理生化响应[J].生态学报,2018,38(7):121-130.
[2]MICHAILIDES T J,ELMER PAG.Botrytis gray mold of kiwifruit caused by Botrytis cinerea in the United States and New Zealand[J].Plant Disease,2000,84(3):208-223.
[3]KULAKIOTUEK,THANASSOULOPOULOSCC,SFAKIOTAKIS E M.Postharvest biological control of Botrytis cinerea on kiwifruit by volatiles of Isabella grapes[J].Phytopathology,2004,94(12):1280-1285.
[4]刘一鸣,杨智仙,董艳.对羟基苯甲酸胁迫下间作对蚕豆枯萎病发生和根系抗氧化酶活性的影响[J].核农学报,2017,31(5):987-995.
[5]覃瀚仪,李魏,戴良英.植物代谢产物在抗病反应中的功能研究进展[J].中国农学通报,2015,31(18):256-259.
[6]SOUZARP,MACHADOEC,SILVA J A B,etal.Photosynthetic gas exchange,chlorophyll fluorescence and some associated metabolic changes in cowpea(Vigna unguiculata)during water stress and recovery[J].Environmental and Experimental Botany,2004,51(1):45-56.
[7]何建社,张利,刘千里,等.岷江干旱河谷区典型灌木对干旱胁迫的生理生化响应[J].生态学报,2018,38(7):2362-2371.
[8]张笑宇,于肖夏,琚亮亮,等.黑痣病菌毒素对马铃薯幼苗生理生化抗性相关物质的诱导[J].植物保护学报,2013,40(1):51-55.
[9]裴斌,张光灿,张淑勇,等.土壤干旱胁迫对沙棘叶片光合作用和抗氧化酶活性的影响[J].生态学报,2013,33(5):1386-1396.
[10]CHEN H Z,CHENG Z,WISNIEWSKI M,et al.Ecofriendly hot water treatment reduces postharvest decay and elicits defense response in kiwifruit[J].Environmental Science and Pollution Research,2015,22(19):15037-15045.
[11]TANG J M,LIU Y Q,LI H H,et al.Combining an antagonistic yeast with harpin treatment to control postharvest decay of kiwifruit[J].Biological Control,2015,89:61-67.
[12]ZHU Y Y,YU J,BRECHT J K,et al.Pre-harvest application of oxalic acid increases quality and resistance to Penicillium expansum in kiwifruit during postharvest storage[J].Food Chemistry,2016,190:537-543.
[13]李忠光,李江鸿,杜朝昆,等.在单一提取系统中同时测定五种植物抗氧化酶[J].云南师范大学学报,2002,22(6):44-48.
[14]CHANCE B,MAEH LYA C.Assay of catalases and peroxidases[J].Methods in Enzymology,1995,2:764-775.
[15]AEBI H.Catalase in vitro[J].Methods in Enzymology,1984,105:121-126.
[16]GIANNOPOLITIS C N,RIES S K.Superoxide dismutases:IOccurrence in higher plants[J].Plant Physiology,1977,59(2):309-314.
[17]高俊凤.植物生理学实验指导[M].北京:高等教育出版社,2006.
[18]JOHNSON D B.The spectrophotometric determiantion of lignin in small wood samples[J].Tappi,1961,44:793-798.
[19]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[20]XUE Z P,FENG W H,CAO J K,et al.Antioxidant activity and total phenolic contents in peel and pulp of Chinese jujube(Ziziphus jujuba Mill)fruits[J].Journal of Food Biochemistry,2009,33(5):613-629.
[21]HUANG Z G,KABIR M H,XIAO Y,et al.Principles and Practice of ABA Analysis[C]//Abscisic Acid:Metabolism,Transport and Signaling.New York and London:Springer Dordrecht Heidelberg,2014.
[22]LIU X,YANG Y L,LIN W H,et al.Determination of both jasmonic acid and methyl jasmonate in plant samples by liquid chromatography tandem mass spectrometry[J].Chinese Science Bulletin,2010,55(21):2231-2235.
[23]张华峰,王志敏,韩娇,等.生姜离体形态发生过程中生理生化指标的变化[J].南方农业,2009,3(3):71-75.
[24]朱诗禹,崔娟,徐伟,等.精异丙甲草胺苗前封闭处理对大豆苗期生长及其生理生化指标的影响[J].植物保护学报,2016,43(4):677-682.
[25]GILL S S,TUTEJA N.Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants[J].Plant Physiology and Biochemistry,2010,48(12):909-930.
[26]王玲利,刘超,曾明,等.采后处理对“黄冠”梨膜脂过氧化和保护酶活性的影响[J].中国南方果树,2017,46(2):132-136.
[27]庞宏东,胡兴宜,胡文杰,等.淹水胁迫对枫杨等3个树种生理生化特性的影响[J].中南林业科技大学学报,2018,38(10):15-21.
[28]刘雪,彭冶,范俊俊,等.淹水胁迫对湖北海棠生长和生理特性的影响[J].经济林研究,2018,36(1):35-42.
[29]张青,彭祚登.七叶树幼苗对水分胁迫和复水的生理响应[J].中南林业科技大学学报,2018,38(4):46-53.
[30]李桦,梁春强,吕茳,等.草酸对冷藏‘华优’猕猴桃果实木质化及相关酶活性的影响[J].园艺学报,2017,44(6):1085-1093.
[31]畅涛,杨成德,薛莉,等.珠芽蓼内生菌ZA1对马铃薯的防病促生研究[J].草业学报,2015,24(12):83-91.
[32]韩庆典,胡晓君,黄坤艳,等.小麦白粉病菌对小麦幼苗MDA含量及防御酶活性的影响[J].分子植物育种,2016,14(10):2803-2811.
[33]韩建东,曹远银.POD和PAL在小麦抗秆锈病中的动态变化[J].江苏农业科学,2009(2):109-110.
[34]孙翠红,徐翠莲,赵铭钦,等.壳寡糖衍生物纳米银微粒诱导烟株产生TMV抗性[J].中国烟草学报,2015,21(6):59-64.
[35]卜冰武,邱德文,曾红梅,等.大丽轮枝菌蛋白激发子PevD1诱导棉花抗病性及作用机理[J].植物病理学报,2014,44(3):254-264.
[36]李伟,熊谨,陈晓阳.木质素代谢的生理意义及其遗传控制研究进展[J].西北植物学报,2003,23(4):675-681.
[37]吴颖静,季浩,唐灿明.大丽轮枝菌侵染拟南芥的过程及诱导的防御反应研究[J].核农学报,2018,32(4):681-689.
[38]BLANCO-ULATE B,VINCENTI E,POWELL A L T,et al.Tomato transcriptome and mutant analyses suggest a role for plant stress hormones in the interaction between fruit and Botrytis cinerea[J].Frontiers in Plant Science,2013,4:142.
[39]何建社,张利,刘千里,等.岷江干旱河谷区典型灌木对干旱胁迫的生理生化响应[J].生态学报,2018,38(7):121-130.