基于HPLC法控制祛屑息风胶囊质量的研究
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摘要
建立祛屑息风胶囊的质量控制方法。色谱柱:Agilent TC-C_(18)(5μm,4.6 mm×250 mm);流动相:水-乙腈(40∶60);检测波长:322 nm;流速:1.0mL/min;柱温:25℃。蛇床子素进样量在0.089 0～0.9771μg与色谱峰面积呈良好的线性关系,平均回收率99.82%,RSD为0.93%。本研究建立的祛屑息风胶囊质量控制方法简单、结果稳定、重复性良好。
The quality control method of Quxiexifeng capsules was established.Chromatographic column:Agilent TC-C_(18)(5μm,4.6 mm×250 mm);water-acetonitrile(40∶60) as mobile phase;detection wavelength was322 nm;flow rate was 1.0 mL/min;column temperature was 25 ℃.In the range of 0.089 0-0.977 1μg of osthol,there was a good linear relationship between the injection volume and the peak area of chromatogram,with an average recovery of 99.82% and RSD of 0.93%.The quality control method of Quxiexifeng capsule established in this study is simple,stable and reproducible.
The quality control method of Quxiexifeng capsules was established.Chromatographic column:Agilent TC-C_(18)(5μm,4.6 mm×250 mm);water-acetonitrile(40∶60) as mobile phase;detection wavelength was322 nm;flow rate was 1.0 mL/min;column temperature was 25 ℃.In the range of 0.089 0-0.977 1μg of osthol,there was a good linear relationship between the injection volume and the peak area of chromatogram,with an average recovery of 99.82% and RSD of 0.93%.The quality control method of Quxiexifeng capsule established in this study is simple,stable and reproducible.
引文
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[2]张嘉明,易增兴,林世清,等.蛇床子素对髓核致炎神经根痛大鼠脊髓背角p38MAPK表达的影响[J].中国药理学通报,2018,34(2):237-243.
[3]吴海璇,冯璐璐,徐辉,等.蛇床子素对髓核致神经根炎性痛大鼠ERK/MAPK信号通路及COX-2 mRNA表达的影响[J].中国药理学通报,2014,(8):1096-1100,1101.
[4]谭晓虹,田嘉铭,赵江垒,等.蛇床子素体外抗氧化活性研究[J].中成药,2013,35(5):1070-1072.
[5]汪涛,赵雁平,胡卫东,等.龙藤筋骨片中蛇床子素含量测定研究[J].中国药物应用与监测,2016,13(2):89-91.
[6]熊琳,朱军,李鹏,等.HPLC测定百草妇炎清栓中蛇床子素的含量[J].中国药师,2016,19(2):365-366.