小熊猫源大肠杆菌病的病理诊断与病原鉴定
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摘要
大肠埃希氏菌的致病性血清型菌株可引起幼龄动物严重的腹泻与败血症。采用常规病理学方法,剖检死亡小熊猫(Ailurus fulgens)并取肠道和实质器官做组织学观察,发现其病变特征为坏死性肠炎及败血症。从肝、脾、肾中无菌分离培养出致病菌,经革兰氏染色及16S rDNA测序鉴定为大肠杆菌。经小鼠毒力实验测得该菌半数致死量LD_(50)为6. 5×10~7CFU,并且从攻毒小鼠脏器分离得到了相同的大肠杆菌。结论:该小熊猫致死原因为致病性大肠杆菌感染。
The pathogenic serotype of Escherichia coli can cause severe diarrhea and sepsis in young animals. Classical pathological method was used to dissect the dead red panda and take intestinal tract and parenchymal organs for histological observation. The pathological features were found to be necrotizing enteritis and septicemia. Pathogenic bacteria were isolated and cultured aseptically from liver,spleen and kidney,and identified as Escherichia coli by gram staining and 16 S rDNA sequencing. The LD_(50) was 6. 5 × 10~7 CFU determined by mouse virulence test,and the same Escherichia coli was isolated from the organs of the challenged mice. It was comprehensively determined that the death of the red panda was pathogenic Escherichia coli infection.
The pathogenic serotype of Escherichia coli can cause severe diarrhea and sepsis in young animals. Classical pathological method was used to dissect the dead red panda and take intestinal tract and parenchymal organs for histological observation. The pathological features were found to be necrotizing enteritis and septicemia. Pathogenic bacteria were isolated and cultured aseptically from liver,spleen and kidney,and identified as Escherichia coli by gram staining and 16 S rDNA sequencing. The LD_(50) was 6. 5 × 10~7 CFU determined by mouse virulence test,and the same Escherichia coli was isolated from the organs of the challenged mice. It was comprehensively determined that the death of the red panda was pathogenic Escherichia coli infection.
引文
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[2]刘峰,张进,刘毅.猪大肠杆菌病防治研究进展[J].湖南畜牧兽医,2010(6):1-3.
[3]陈甜甜,杨忠福,刘建柱.猪大肠杆菌病研究进展[J].猪业科学,2008(8):20-25.
[4]那春子.野生动物大肠杆菌病的临床表现及防治[J].现代畜牧科技,2017(11):120.
[5]YANG Q,SHI Y F,CHEN J,et al.Isolation,identification of escherichia coli from mink and related research of some biological characteristics[J].Chinese Journal of Veterinary Drug,2015(8):14-19.
[6]张伟,王家励.鹿大肠杆菌病的诊治[J].今日畜牧兽医,2015(12):61.
[7]林成水,谢一俊,陈元川,等.大肠杆菌引起大熊猫急性出血性肠炎[J].中国兽医杂志,1992(9):7-8.
[8]芦静,苏朋成,娄安钢.1例长白山野杂猪大肠杆菌病的病理学观察[J].畜禽业,2018(8):19.
[9]陆倩倩,彭欠欠,刘阳,等.1株孔雀源大肠杆菌的分离与药敏试验[J].养禽与禽病防治,2013(5):41-42.
[10]刘从明.大雁大肠杆菌病的诊治[J].畜牧与兽医,2011(9):107.
[11]汪松.中国濒危动物红皮书,兽类[M].北京:科学出版社,1998:166.
[12]胡洪光,沈庆永.小熊猫在中国的饲养历史及现状[J].野生动物,1997(6):16-18.
[13]关春宇.浅谈圈养野生动物疾病及发病原因[J].中国畜牧兽医文摘,2018(3):185.
[14]李明杰.1例小熊猫大肠杆菌性脓血症的诊断[J].畜牧与兽医,2012(11):112.
[15]吴宣,杨泽林.小熊猫犬瘟热继发大肠杆菌的诊治[J].四川畜牧兽医,2003(6):48.
[16]朱煜兰,周学文,金致昆.小熊猫巴氏杆菌大肠杆菌沙门氏菌混合感染的诊疗[J].中国兽医科技,1997(9):34-35.
[17]LORD J M,MIDWINTER M J,CHEN Y F,et al.The systemic immune response to trauma:An overview of pathophysiology and treatment[J].The Lancet,2014,384(9952):1455-1465.
[18]李心坦,曹晋蓉.抗生素对动物免疫功能影响的研究[J].黑龙江畜牧兽医,1995(7):22-23.
[19]任志娟,许晓军,郭子文,等.血液病患者院内感染大肠埃希菌败血症临床分析[J].海南医学,2012(14):101-103.
[20]MARIE A M B,WEE M Y K,HADDOCK B A.An improved method for the identification and characterization of mutants of Escherichia coli deficient in formate dehydrogenase activity[J].FEMS Microbiology Letters,2010,4(1):37-40.
[21]刘玉芹,张秀英,朱琪.仔猪黄白痢防治的研究进展[J].黑龙江畜牧兽医,2001(7):22-23.
[22]梁少宇.羔羊大肠杆菌病的诊治措施[J].畜牧兽医科技信息,2018(1):79.
[23]李健.犊牛大肠杆菌腹泻病的综合防治[J].山东畜牧兽医,2015(7):27.
[24]高齐瑜,曹澍泽,刘瑞萍.野生猫科动物大肠杆菌病[J].中国兽医杂志,1987(9):25-26.
[25]范光丽,杨增歧,高更更,等.朱鹮幼雏大肠杆菌病的病理组织学观察[J].动物学杂志,2004,39(3):44-46.
[26]CHEN Q,YU S Y,SHEN H,et al.Pathogenic mechanisms of enterotoxigenic Escherichia coli in guinea pigs[J].Journal of First Military Medical University,2003,23(8):826-829.
[27]吕志生.鸡新城疫与大肠杆菌病混感的诊治[J].畜牧兽医科技信息,2018(9):145-146.
[28]杨阳.野生动物大肠杆菌病的诊断与防治[J].现代畜牧科技,2017(1):124.
[29]丁红雷,毛旭虎,王豪举,等.肠出血性大肠杆菌O157的实验室分离与鉴定方法[J].卫生研究,2007,36(2):242-244.
[30]王世民,苏艳,吐尔洪·努尔.新疆奶牛乳源大肠杆菌毒力相关基因eae A的克隆及序列分析[J].新疆农业大学学报,2011,34(2):152-155.
[31]马章林,唐曙明,楼许柏.16SrRNA在医学微生物鉴定中的应用探讨[J].吉林医学,2011,32(12):2291-2292.
[32]DELASKI K M,RAMSAY E,GAMBLE K C.Retrospective analysis of mortality in the north American captive red panda(Ailurus fulgens)population[J].Journal of Zoo and Wildlife Medicine,2015,46(4):779-788.
[33]QIN Q,WEI F W,LI M,et al.Serosurvey of infectious disease agents of carnivores in captive red pandas(Ailurus fulgens)in China[J].Journal of Zoo and Wildlife Medicine,2007,38(1):42.