土炒白术HPLC指纹图谱研究
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摘要
目的 :建立土炒白术的HPLC指纹图谱,为全面控制土炒白术的质量提供方法。方法 :采用Brava BDS C18(250 mm×4.6 mm,5μm)色谱柱;甲醇(A)-水(B)梯度洗脱;流速:1.0 m L·min-1;检测波长:220 nm,建立土炒白术的HPLC指纹图谱。结果 :标定了土炒白术中的14个共有峰,非共有峰面积小于10%;10批药材成分的相似度较高,RSD为0.902%~0.997%。结论:该方法简便,重复性好,特征性强,可用于土炒白术药材的鉴别和质量控制。
Objective:To establish the fingerprints and provide the method for the quality control of soil-processed Atractylodis macrocephalae Rhizoma by HPLC. Methods:The separation was performed on Brava BDS C18column(250 mm×4.6 mm,5 μm),with methanol(A)-water(B) as mobile phase by gradient elution,the flow rate was 1.0 m L·min-1,the detection wavelength was 220 nm. Results:Total 14 common peaks were selected in soil-processed Atractylodis macrocephalae Rhizoma,the non-common peak area was less than 10%,the ten batches of herbs were with very high similarity,RSD was 0.902% ~0.997%. Conclusion:The method was convenient and with high repeatability and characteristic,could be used for the identification and quality control of soil-processed Atractylodis macrocephalae Rhizoma.
Objective:To establish the fingerprints and provide the method for the quality control of soil-processed Atractylodis macrocephalae Rhizoma by HPLC. Methods:The separation was performed on Brava BDS C18column(250 mm×4.6 mm,5 μm),with methanol(A)-water(B) as mobile phase by gradient elution,the flow rate was 1.0 m L·min-1,the detection wavelength was 220 nm. Results:Total 14 common peaks were selected in soil-processed Atractylodis macrocephalae Rhizoma,the non-common peak area was less than 10%,the ten batches of herbs were with very high similarity,RSD was 0.902% ~0.997%. Conclusion:The method was convenient and with high repeatability and characteristic,could be used for the identification and quality control of soil-processed Atractylodis macrocephalae Rhizoma.
引文
[1]国家药典委员会.中华人民共和国药典[S].一部.北京:中国医药科技出版社,2010:348.
[2]于永明,贾天柱,梁武学,等.白术炮制的历史沿革[J].辽宁中医学院学报,2005(6):635-636.
[3]李庆.白术的不同炮制法对临床作用的影响[J].中国医药指南,2013,19:302-303.
[4]崔小兵,李伟,文红梅,等.UPLC法测定白术不同炮制品中5-羟甲基糠醛及糠醛的含量[J].南京中医药大学学报,2012,28(5):477-478.
[2]于永明,贾天柱,梁武学,等.白术炮制的历史沿革[J].辽宁中医学院学报,2005(6):635-636.
[3]李庆.白术的不同炮制法对临床作用的影响[J].中国医药指南,2013,19:302-303.
[4]崔小兵,李伟,文红梅,等.UPLC法测定白术不同炮制品中5-羟甲基糠醛及糠醛的含量[J].南京中医药大学学报,2012,28(5):477-478.