摘要
[目的]香樟齿喙象是近年来发现的一种危害香樟的单食性蛀干害虫,为探索其系统发育地位和遗传进化关系,本研究分别扩增香樟齿喙象的mt DNA COI、18S rDNA V4、V7区和28S rDNA D2区序列。[方法]采用邻接法(neighbour-joining)和极大似然法(maximum likelihood)构建不同序列的系统发育树,分析香樟齿喙象与象甲科Curculionidae其它种类同源序列的碱基组成及系统进化特点,从分子水平探讨香樟齿喙象的分类地位。[结果]表明:COI与rDNA均显示香樟齿喙象与同科同族的魔喙象亚科Molytinae、树皮象族Hylobiini种类遗传距离最为相近,虽然无法比对到同属下的种类,但支持香樟齿喙象归为魔喙象亚科树皮象族的观点。[结论]通过比较发现,28S rDNA序列分析结果印证了香樟齿喙象目前的分类地位,本研究为香樟齿喙象在分子水平上的鉴定提供了理论依据。
[Objective] To analyze the phylogenetic position and genetic evolution of Pagiophloeus tsushimanus.[Method]FourDNA sequence fragments: cytochrome oxidase subunitⅠ(COI) gene of mitochondrial DNA(mtDNA),V4 and V7 region of 18 S ribosomal DNA(18 S rDNA) and D2 region of 28 S ribosomal DNA(28 S rDNA)were amplified. Maximum likelihood(ML) and neighbor jointing(NJ) methods were used to build up phylogenetic trees of P. tsushimanus and related species in Curculionidae,so as to study the classification status of P. tsushimanus. [Result]Analysis on COI and rDNA suggested that P. tsushimanus belongs to Hylobiini of Molytinae. [Conclusion]According to the analysis,the results of 28 S rDNA support the morphological classification of P. tsushimanus.
引文
[1]李巧.中国象甲科分类研究综述[J].西南林学院学报,2003,23(3):74-79.
[2]赵艳,高晓余,肖春.象甲科昆虫寄主选择行为研究进展[J].农业灾害研究,2014,4(7):8-21,46.
[3]黄俊浩,吴时英,高磊,等.中国新记录种——香樟齿喙象的鉴别与为害[J].浙江农林大学学报,2014,31(5):764-767.
[4]Alonsozarazaga M A,Lyal C H C.A world catalogue of families and genera of Curculionoidea(Insecta:Coleoptera)(Excepting Scolytidae and Platypodidae)[M].Barcelona Entomopraxis,1999:202.
[5]Faust J.Curculionidenaus dem Malayischen Archipel[J].Stettiner Entomologische Zeitung,1892,53(7/9):184-228.
[6]Hebert P D N,Cywinska A,Ball S L,et al.Biological identifications through DNA barcodes[J].Proceedings Biological Sciences,2003,270(1512):313-321.
[7]Hebert P D,Ratnasingham S,Dewaard J R.Barcoding animal life:cytochrome c oxidase subunit 1 divergences among closely related species[J].Proceedings of the Royal Society Biological Sciences,2003,270(Suppl-1):S96-S99.
[8]魏子涵,尹新明,安世恒,等.利用分子标记18S r DNA对天牛高阶元进化关系的研究[J].应用昆虫学报,2014,51(02):425-439.
[9]Marvaldi A E.Higher level phylogeny of Curculionidae(Coleoptera:Curculionoidea)based mainly on larval characters,with special reference to broad-nosed weevils[J].Cladistics,1997,13(4):285-312.
[10]Marvaldi A E,Morrone J J.Phylogenetic systematics of weevils(Coleoptera:Curculionoidea):A reappraisal based on larval and adult morphology[J].Insect Systematics and Evolution,2000,31(1):43-58.
[11]Marvaldi A E,Sequeira A S,O'Brien C W,et al.Molecular and morphological phylogenetics of weevils(Coleoptera,Curculionoidea):do niche shifts accompany diversification?[J].Systematic Biology,2002,51(5):761-785.
[12]黄华平,杨腊英,王国芬,等.r DNA和mt DNA在昆虫系统发育与区系研究中的应用[J].热带生物学报,2006,12(4):45-49.
[13]张健,李晓强,刘斐,等.基于线粒体16S r DNA基因的天牛科部分种类分子系统学研究(鞘翅目:天牛科)[J].昆虫分类学报,2010(S1):1-7.
[14]张健,张晓军,任炳忠.基于28S r DNA基因的天牛科部分种类的分子系统发育[J].林业科学,2012,48(10):86-94.
[15]王志良,张润志.小蠹亚科的分类地位(鞘翅目,象虫科)[J].动物分类学报,2012,37(2):291-295.
[16]栾丰刚,丁俊杰,何龙喜,等.以线粒体COⅠ基因探讨萧氏松茎象的分类地位[J].江西农业大学学报,2013,35(5):906-913.
[17]Loxdale H D,Lushai G.Molecular markers in entomology[J].Bulletin of Entomological Research,1998,88(06):577-600.
[18]Raupach M J,Astrin J J,Hannig K,et al.Molecular species identification of Central European ground beetles(Coleoptera:Carabidae)using nuclear r DNA expansion segments and DNA barcodes[J].Frontiers in Zoology,2010,7(1):26.
[19]Gillespie J J,Kjer K M,Duckett C N,et al.Convergent evolution of cucurbitacin feeding in spatially isolated rootworm taxa(Coleoptera:Chrysomelidae;Galerucinae,Luperini)[J].Molecular Phylogenetics and Evolution,2003,29(1):161-175.
[20]Tamura K,Peterson D,Peterson N,et al.MEGA5:molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Molecular Biology and Evolution,2011,28(10):2731-2739.
[21]Parchman T L,Geist K S,Grahnen J A,et al.Transcriptome sequencing in an ecologically important tree species:assembly,annotation,and marker discovery[J].BMC Genomics,2010,11(1):180.
[22]Rota-Stabelli O,Telford M J.A multi criterion approach for the selection of optimal outgroups in phylogeny:Recovering some support for Mandibulata over Myriochelata using mitogenomics[J].Molecular Phylogenetics and Evolution,2008,48(1):103-111.
[23]Chenna R,Sugawara H,Koike T,et al.Multiple sequence alignment with the clustal series of programs[J].Nucleic Acids Research,2003,31(13):3497-3500.
[24]常虹,郝德君,肖荣堂,等.基于线粒体COⅠ基因的齿小蠹属昆虫DNA条形码研究[J].昆虫学报,2012,55(9):1075-1081.
[25]聂瑞娥,杨星科.鞘翅目昆虫线粒体基因组研究进展[J].昆虫学报,2014,57(7):860-868.
[26]贾晨曦.我国北方金龟科甲虫分子标记和系统发育研究[D].沈阳,沈阳大学,2014.
[27]Geiser M F,Hagmann R,Nagel P,et al.A first broad-scale molecular phylogeny of Prionoceridae(Coleoptera:Cleroidea)provides insight into taxonomy,biogeography and life history evolution[J].Arthropod Systematics&Phylogeny,2016,74(1).
[28]Lorenz J G,Jackson W E,Hanner R.The problems and promise of DNA barcodes for species diagnosis of primate biomaterials[J].Philosophical Transactions of the Royal Society B Biological Sciences,2005,360(1462):1869-1877.
[29]Xie L,Miao H,Hong X Y.The Two-spotted spider mite Tetranychus urticae Koch and the carmine spider mite Tetranychus cinnabarinus(Boisduval)in China mixed in their Wolbachia phylogenetic tree[J].Zootaxa,2006,1165:3346.
[30]杨瑞生,钟亮,姜义仁,等.橡实象虫等25种昆虫线粒体COI基因的遗传多样性及系统进化分析[J].蚕业科学,2011,37(06):985-992.
[31]孙田依.锈赤扁谷盗和土耳其扁谷盗线粒体基因组特征及系统发育研究[D].江西,南昌大学,2016.