水稻亲本籼粳分化程度与杂种优势的相关性
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摘要
【目的】分析水稻亲本籼粳分化程度与杂种优势的相关性,为研究双亲籼粳分化最佳配比及组配籼粳交强优势组合提供理论参考。【方法】采用ILP分子标记和程氏形态指数法对51份水稻亲本材料进行籼粳分化程度检测,基于23个ILP分子标记进行系统聚类分析,分析两种方法的相关性。按籼/籼、偏籼/偏籼、籼/偏籼、籼/偏粳、偏籼/偏粳和偏粳/偏粳6种类型配制150个杂交组合,综合分析亲本籼粳分化程度与杂种优势的相关性。【结果】采用程氏形态指数法从51份水稻亲本材料共检测出籼型20份、偏籼型19份、偏粳型6份和粳型6份;利用23对扩增结果稳定、条带清晰且只有两种等位基因的ILP引物从51份供试亲本材料中共检测出籼型14份、偏籼型20份、偏粳型11份和粳型6份,不存在100%纯籼或纯粳类型。ILP分子标记法与程氏形态指数法的籼粳分类结果均与系统聚类分析结果具有较高的吻合度,但仍在差异。51份供试亲本的粳稻成分指数与穗轴第1～2节长和抽穗时稃色呈显著正相关(P<0.05),与谷粒长宽比、酚反应和程氏指数总分呈极显著正相关(P<0.01),其中与程氏指数总分的相关系数最高,为0.81。随着组配双亲粳稻成分的增加,6种组配类型杂交组合的生育期、株高和每穗总粒数总体呈增加趋势,但单株有效穗数、结实率和千粒重总体呈下降趋势,单株重则呈先增加再下降的变化趋势。【结论】利用ILP分子标记可有效区分亲本的籼粳属性,是一种有效的水稻籼粳分类工具。采用籼/偏籼方式配组较易筛选到强优组合;虽然采用籼/偏粳方式配组获得高产组合的概率较低,但有机会获得极高产组合,在育种中亦可大胆尝试。
【Objective】The correlation between indica-japonica differentiation and heterosis was studied to provide theoretical reference for the optimum ratio of indica-japonica differentiation in parents of indica-japonica rice and intersubspecific hybrid rice combinations.【Method】ILP molecular marker and Cheng's morphology index were used to detect indica-japonica differentiation level of 51 rice parents. And the correlation between the two methods was clustered by 23 ILP molecular markers. A total of 150 hybrid combinations were set based on indica/indica,partial indica/partial indica,indica/partial indica,indica/partial japonica,partial indica/partial japonica and partial japonica/partial japonica,and correlation between indica-japonica differentiation and heterosis was analyzed.【Result】There were 20 indica types,19 partial indica types,6 partial japonica types and 6 japonica types were detected from the 51 tested parents based on Cheng's morphology index. Twenty-three pairs of ILP primers which had stable amplification products,clear bands and two alleles were used to detect 14 indica types,20 partial indica types,11 partial japonica types and 6 japonica types out of 51 tested parents. There was no 100% pure indica or japonica types. The indica-japonica differentiation results by ILP molecular marker and Cheng's morphology index were highly consistent with clustering analysis but still different. Indica-japonica component index of 51 test parents was significantly positively correlated with length of the first and second rachis internode and glume color at heading stage(P<0.05),and was extremely positively correlated with length-width ratio of grain,phenol reaction and Cheng's index total score(P<0.01). The correlation with Cheng's index total score was the highest(0.81). With the increase of parental japonica rice composition,the six types combinations all showed an overall increasing trend in growth period,plant height and total grain per panicle. But effective panicles per plant,seed-setting rate and 1000-grain weight decreased. The variation trend of single plant weight was firstly increased and then decreased.【Conclusion】ILP molecular marker can be used to accurately detect the proportion of indica/japonica content in rice parent. It is an easy tool to classify indica and japonica rice. Fine combinations can be easily obtained through indica/partial indica combination. It is possible to obtain extremely high-yield combinations through indica/partial japonica,although the probability of obtaining high-yield combinations is low. It is worth trying in breeding.
【Objective】The correlation between indica-japonica differentiation and heterosis was studied to provide theoretical reference for the optimum ratio of indica-japonica differentiation in parents of indica-japonica rice and intersubspecific hybrid rice combinations.【Method】ILP molecular marker and Cheng's morphology index were used to detect indica-japonica differentiation level of 51 rice parents. And the correlation between the two methods was clustered by 23 ILP molecular markers. A total of 150 hybrid combinations were set based on indica/indica,partial indica/partial indica,indica/partial indica,indica/partial japonica,partial indica/partial japonica and partial japonica/partial japonica,and correlation between indica-japonica differentiation and heterosis was analyzed.【Result】There were 20 indica types,19 partial indica types,6 partial japonica types and 6 japonica types were detected from the 51 tested parents based on Cheng's morphology index. Twenty-three pairs of ILP primers which had stable amplification products,clear bands and two alleles were used to detect 14 indica types,20 partial indica types,11 partial japonica types and 6 japonica types out of 51 tested parents. There was no 100% pure indica or japonica types. The indica-japonica differentiation results by ILP molecular marker and Cheng's morphology index were highly consistent with clustering analysis but still different. Indica-japonica component index of 51 test parents was significantly positively correlated with length of the first and second rachis internode and glume color at heading stage(P<0.05),and was extremely positively correlated with length-width ratio of grain,phenol reaction and Cheng's index total score(P<0.01). The correlation with Cheng's index total score was the highest(0.81). With the increase of parental japonica rice composition,the six types combinations all showed an overall increasing trend in growth period,plant height and total grain per panicle. But effective panicles per plant,seed-setting rate and 1000-grain weight decreased. The variation trend of single plant weight was firstly increased and then decreased.【Conclusion】ILP molecular marker can be used to accurately detect the proportion of indica/japonica content in rice parent. It is an easy tool to classify indica and japonica rice. Fine combinations can be easily obtained through indica/partial indica combination. It is possible to obtain extremely high-yield combinations through indica/partial japonica,although the probability of obtaining high-yield combinations is low. It is worth trying in breeding.
引文
陈芬,陈雨,曲延英,潘大建,杨庆文,井赵斌,刘维,范芝兰,陈建酉,李晨.2009.粤北普通野生稻籼粳分化的SSR分析[J].基因组学与应用生物学,28(2):51-56.[Chen F,Chen Y,Qu Y Y,Pan D J,Yang Q W,Jing Z B,Liu W,Fan Z L,Chen J Y,Li C.2009.SSR analysis of indicajaponica differentiation of Oryza rufipogon Griff.in Yuebei[J].Genomics and Applied Biology,(2):51-56.]
陈惠清,黄荣裕,王天生,谢旺有,陈锦文,谢少和,吴建梅,林荔辉.2016.水稻籼粳交后代的籼粳分化与杂种F1结实率的相关分析[J].福建农业学报,31(3):211-216.[Chen H Q,Huang R Y,Wang T S,Xie W Y,Chen J W,Xie S H,Wu J M,Lin L H.2016.Correlation between F1seed-setting and genetic differentiation of indica japonica progenies[J].Fujian Journal of Agricultural Sciences,31(3):211-216.]
程侃声.1993.亚洲稻籼粳亚种的鉴别[M].昆明:云南科技出版社.[Cheng K S.1993.Indentificating of Subspecies of Indica and Japonica in Oryza sativa L.[M].Kunming:Science Publishing House of Yunnan Press.]
范方军,王妍,王芳权,王军,朱金燕,李文奇,仲维功,胡海燕,杨杰.2015.太湖流域水稻地方品种籼粳分化和遗传多样性分析[J].江苏农业科学,43(12):68-73.[Fan FJ,Wang Y,Wang F Q,Wang J,Zhu J Y,Li W Q,Zhong W G,Hu H Y,Yang J.2015.Analysis on indica-japonica differentiation and genetic diversity of rice varieties from Taihu lake basin[J].Jiangsu Agricultural Sciences,43(12):68-73.]
黄明.2010.分子标记与水稻遗传多样性及杂种优势的相关性研究[D].长沙:湖南农业大学.[Huang M.2010.Correlation analysis of molecular markers and genetic diversity and heterosis in rice[D].Changsha:Hunan Agricultural University.]
姜树坤,钟鸣,徐正进,张丽,马慧,刘少霞.2006.基于RAPD标记进行水稻籼粳分类研究[J].沈阳农业大学学报,37(4):639-644.[Jiang S K,Zhong M,Xu Z J,Zhang L,Ma H,Liu S X.2006.Classification of rice cultivars with RAPD molecular markers[J].Journal of Shenyang Agricultural University,37(4):639-644.]
姜廷波,孙传清,李任华,李自超,王象坤.1999.利用RFLP标记对两系杂交水稻及其亲本的分类研究[J].中国农业科学,32(6):8-15.[Jiang T B,Sun C Q,Li R H,Li Z C,Wang X K.1999.Study on classification of crosses and their parents by RFLP markers in two-line system hybrid rice[J].Scientia Agricultura Sinica,32(6):8-15.]
况浩池,曾正明,刘国民,文绍山,罗俊涛,陈光珍,杨扬.2007.水稻耐热、高配合力籼粳交恢复系泸恢17的创制与应用[J].种子,(10):60-63.[Kuang H C,Zeng Z M,Liu G M,Wen S S,Luo J T,Chen G Z,Yang Y.2007.Creation and application of the heat tolerance and high combining ability indica japonica rice restorer line Luhui17[J].Seed,(10):60-63.]
李亚莉,马继武,杨晓曦,许明辉,赵丰萍.2012.利用SSR分析滇型杂交稻亲本籼粳分化与杂种优势的关系[J].西南农业学报,25(2):347-353.[Li Y L,Ma J W,Yang XX,Xu M H,Zhao F P.2012.Analysis of relationship between indica-japonica differentiation of parents and heterosis in Dian type hybrid rice by SSR markers[J].Southwest China Journal of Agricultural Sciences,25(2):347-353.]
龙雯虹,许明辉,张树华.2003.滇型杂交稻亲本籼粳分化与杂种优势关系的初步研究[J].中国农业科学,36(1):1-6.[Long W H,Xu M H,Zhang S H.2003.A preliminary study on the relationship between the indica-japonica differentiation of parents and heterosis in dian type hybrid rice by RAPD markers[J].Scientia Agricultura Sinica,36(1):1-6.]
马彬林.2015.籼粳衍生恢复系的研制与利用[J].福建农业学报,30(4):322-325.[Ma B L.2015.Development and utilization of the restorer lines derived from indica-japonica cross[J].Fujian Journal of Agricultural Sciences,30(4):322-325.]
孙传清,姜廷波,陈亮,吴长明,李自超,王象坤.2000.水稻杂种优势与遗传分化关系的研究[J].作物学报,26(6):641-649.[Sun C Q,Jiang T B,Chen L,Wu C M,Li Z C,Wang X K.2000.Studies on the relationship between heterosis and genetic differentiation in hybrid rice(Oryza sativa L.)[J].Acta Agronomica Sinica,26(6):641-649.]
王明军,王云月,陆春明,杨慧,王云涛.2010.利用籼粳稻特异InDel标记分析云南糯稻品种的籼粳特性[J].云南农业大学学报(自然科学版),25(3):333-337.[Wang M J,Wang Y Y,Lu C M,Yang H,Wang Y T.2010.The indicajaponica characteristics of yunnan glutinous rice varieties were analyzed by indica-japonica InDel markers[J].Journal of Yunnan Agricultural University(Natural Science),25(3):333-337.]
徐海,刘宏光,杨莉,朱春杰,王嘉宇,杨乾华,徐正进,郑家奎.2007.不同生态条件下籼粳稻杂交后代亚种特性的比较研究[J].作物学报,33(3):370-377.[Xu H,Liu HG,Yang L,Zhu C J,Wang J Y,Yang Q H,Xu Z J,Zheng J K.2007.Subspecies characteristics in filial generation of cross between indica and japonica under different environments[J].Acta Agronomica Sinica,33(3):370-377.]
许旭明,梁康迳,张受刚,尚伟,张瑛英,韦新宇,柯蓓.2009.利用ILP标记分析水稻籼粳杂交亲本和衍生系的籼粳分化[J].中国农业科学,42(10):3388-3396.[Xu X M,Liang K J,Zhang S G,Shang W,Zhang Y Y,Wei X Y,Ke B.2009.Analysis of indica-japonica differentiation in rice parents and derived lines using ILP markers[J].Scientia Agricultura Sinica,42(10):3388-3396.]
杨旺兴,许旭明,祁建民,卓伟,张受刚,马彬林,韦新宇,邹文广.2013.水稻籼粳交衍生系籼粳分化分析及其在DNA水平上划分标准初探[J].西南农业学报,26(4):1301-1307.[Yang W X,Xu X M,Qi J M,Zhuo W,Zhang SG,Ma B L,Wei X Y,Zou W G.2013.Analysis of indica-japonica classification in rice derived lines and division standard in DNA level[J].Southwest China Journal of Agricultural Sciences,26(4):1301-1307.]
袁隆平,武小金,颜应成,罗孝和.1997.水稻广谱广亲和系的选育策略[J].中国农业科学,30(4):1-8.[Yuan L P,Wu XJ,Yan Y C,Luo X H.1997.A strategy for developing wide spectrum compatibility rice line[J].Scientia Agricultura Sinica,30(4):1-8.]
张建勇,袁佐清,李仕贵.2005.微卫星标记分析籼粳亚种间的遗传多样性[J].山东理工大学学报,9(2):22-27.[Zhang J Y,Yuan Z Q,Li S G.2005.The genetic diversity and relationship between indica and japonica rice(Oryza sativa L.)using SSR markers[J].Journal of Shandong University of Technology,9(2):22-27.]
张受刚,许旭明.2006.粳型亲籼恢复系的选育与利用[J].福建稻麦科技,24(1):3-5.[Zhang S G,Xu X M.2006.Breeding and utilization indica compatible restorer lines[J].Fujian Science and Technology of Rice and Wheat,24(1):3-5.]
郑秀娟,解慧芳,曹红瑞,杨桂英,谢长荣,梁康迳,孙新立.2016.水稻籼粳亚种分化关联性状的QTL分析[J].福建农林大学学报(自然科学版),45(1):1-7.[Zheng X J,Xie H F,Cao H R,Yang G Y,Xie C R,Liang K J,Sun X L.2016.QTL analysis of the differentiation characteristics of indica and japonica rice and associated traits[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),45(1):1-7.]
左生力.2016.几个籼型两系杂交稻亲本的配合力及遗传力分析[J].福建农业学报,31(12):1299-1307.[Zuo S L.2016.Analysis on combining ability and heritability of 5new PTGMS lines and 6 new restorer lines in Indica hybrid rice[J].Fujian Journal of Agricultural Sciences,31(12):1299-1307.]
Li R H,Jiang T B,Xu C G,Li X H,Wang X K.2000.Relationship between morphological and genetic differentiation in rice(Oryza sativa L.)[J].Euphytica,114(1):1-8.
陈惠清,黄荣裕,王天生,谢旺有,陈锦文,谢少和,吴建梅,林荔辉.2016.水稻籼粳交后代的籼粳分化与杂种F1结实率的相关分析[J].福建农业学报,31(3):211-216.[Chen H Q,Huang R Y,Wang T S,Xie W Y,Chen J W,Xie S H,Wu J M,Lin L H.2016.Correlation between F1seed-setting and genetic differentiation of indica japonica progenies[J].Fujian Journal of Agricultural Sciences,31(3):211-216.]
程侃声.1993.亚洲稻籼粳亚种的鉴别[M].昆明:云南科技出版社.[Cheng K S.1993.Indentificating of Subspecies of Indica and Japonica in Oryza sativa L.[M].Kunming:Science Publishing House of Yunnan Press.]
范方军,王妍,王芳权,王军,朱金燕,李文奇,仲维功,胡海燕,杨杰.2015.太湖流域水稻地方品种籼粳分化和遗传多样性分析[J].江苏农业科学,43(12):68-73.[Fan FJ,Wang Y,Wang F Q,Wang J,Zhu J Y,Li W Q,Zhong W G,Hu H Y,Yang J.2015.Analysis on indica-japonica differentiation and genetic diversity of rice varieties from Taihu lake basin[J].Jiangsu Agricultural Sciences,43(12):68-73.]
黄明.2010.分子标记与水稻遗传多样性及杂种优势的相关性研究[D].长沙:湖南农业大学.[Huang M.2010.Correlation analysis of molecular markers and genetic diversity and heterosis in rice[D].Changsha:Hunan Agricultural University.]
姜树坤,钟鸣,徐正进,张丽,马慧,刘少霞.2006.基于RAPD标记进行水稻籼粳分类研究[J].沈阳农业大学学报,37(4):639-644.[Jiang S K,Zhong M,Xu Z J,Zhang L,Ma H,Liu S X.2006.Classification of rice cultivars with RAPD molecular markers[J].Journal of Shenyang Agricultural University,37(4):639-644.]
姜廷波,孙传清,李任华,李自超,王象坤.1999.利用RFLP标记对两系杂交水稻及其亲本的分类研究[J].中国农业科学,32(6):8-15.[Jiang T B,Sun C Q,Li R H,Li Z C,Wang X K.1999.Study on classification of crosses and their parents by RFLP markers in two-line system hybrid rice[J].Scientia Agricultura Sinica,32(6):8-15.]
况浩池,曾正明,刘国民,文绍山,罗俊涛,陈光珍,杨扬.2007.水稻耐热、高配合力籼粳交恢复系泸恢17的创制与应用[J].种子,(10):60-63.[Kuang H C,Zeng Z M,Liu G M,Wen S S,Luo J T,Chen G Z,Yang Y.2007.Creation and application of the heat tolerance and high combining ability indica japonica rice restorer line Luhui17[J].Seed,(10):60-63.]
李亚莉,马继武,杨晓曦,许明辉,赵丰萍.2012.利用SSR分析滇型杂交稻亲本籼粳分化与杂种优势的关系[J].西南农业学报,25(2):347-353.[Li Y L,Ma J W,Yang XX,Xu M H,Zhao F P.2012.Analysis of relationship between indica-japonica differentiation of parents and heterosis in Dian type hybrid rice by SSR markers[J].Southwest China Journal of Agricultural Sciences,25(2):347-353.]
龙雯虹,许明辉,张树华.2003.滇型杂交稻亲本籼粳分化与杂种优势关系的初步研究[J].中国农业科学,36(1):1-6.[Long W H,Xu M H,Zhang S H.2003.A preliminary study on the relationship between the indica-japonica differentiation of parents and heterosis in dian type hybrid rice by RAPD markers[J].Scientia Agricultura Sinica,36(1):1-6.]
马彬林.2015.籼粳衍生恢复系的研制与利用[J].福建农业学报,30(4):322-325.[Ma B L.2015.Development and utilization of the restorer lines derived from indica-japonica cross[J].Fujian Journal of Agricultural Sciences,30(4):322-325.]
孙传清,姜廷波,陈亮,吴长明,李自超,王象坤.2000.水稻杂种优势与遗传分化关系的研究[J].作物学报,26(6):641-649.[Sun C Q,Jiang T B,Chen L,Wu C M,Li Z C,Wang X K.2000.Studies on the relationship between heterosis and genetic differentiation in hybrid rice(Oryza sativa L.)[J].Acta Agronomica Sinica,26(6):641-649.]
王明军,王云月,陆春明,杨慧,王云涛.2010.利用籼粳稻特异InDel标记分析云南糯稻品种的籼粳特性[J].云南农业大学学报(自然科学版),25(3):333-337.[Wang M J,Wang Y Y,Lu C M,Yang H,Wang Y T.2010.The indicajaponica characteristics of yunnan glutinous rice varieties were analyzed by indica-japonica InDel markers[J].Journal of Yunnan Agricultural University(Natural Science),25(3):333-337.]
徐海,刘宏光,杨莉,朱春杰,王嘉宇,杨乾华,徐正进,郑家奎.2007.不同生态条件下籼粳稻杂交后代亚种特性的比较研究[J].作物学报,33(3):370-377.[Xu H,Liu HG,Yang L,Zhu C J,Wang J Y,Yang Q H,Xu Z J,Zheng J K.2007.Subspecies characteristics in filial generation of cross between indica and japonica under different environments[J].Acta Agronomica Sinica,33(3):370-377.]
许旭明,梁康迳,张受刚,尚伟,张瑛英,韦新宇,柯蓓.2009.利用ILP标记分析水稻籼粳杂交亲本和衍生系的籼粳分化[J].中国农业科学,42(10):3388-3396.[Xu X M,Liang K J,Zhang S G,Shang W,Zhang Y Y,Wei X Y,Ke B.2009.Analysis of indica-japonica differentiation in rice parents and derived lines using ILP markers[J].Scientia Agricultura Sinica,42(10):3388-3396.]
杨旺兴,许旭明,祁建民,卓伟,张受刚,马彬林,韦新宇,邹文广.2013.水稻籼粳交衍生系籼粳分化分析及其在DNA水平上划分标准初探[J].西南农业学报,26(4):1301-1307.[Yang W X,Xu X M,Qi J M,Zhuo W,Zhang SG,Ma B L,Wei X Y,Zou W G.2013.Analysis of indica-japonica classification in rice derived lines and division standard in DNA level[J].Southwest China Journal of Agricultural Sciences,26(4):1301-1307.]
袁隆平,武小金,颜应成,罗孝和.1997.水稻广谱广亲和系的选育策略[J].中国农业科学,30(4):1-8.[Yuan L P,Wu XJ,Yan Y C,Luo X H.1997.A strategy for developing wide spectrum compatibility rice line[J].Scientia Agricultura Sinica,30(4):1-8.]
张建勇,袁佐清,李仕贵.2005.微卫星标记分析籼粳亚种间的遗传多样性[J].山东理工大学学报,9(2):22-27.[Zhang J Y,Yuan Z Q,Li S G.2005.The genetic diversity and relationship between indica and japonica rice(Oryza sativa L.)using SSR markers[J].Journal of Shandong University of Technology,9(2):22-27.]
张受刚,许旭明.2006.粳型亲籼恢复系的选育与利用[J].福建稻麦科技,24(1):3-5.[Zhang S G,Xu X M.2006.Breeding and utilization indica compatible restorer lines[J].Fujian Science and Technology of Rice and Wheat,24(1):3-5.]
郑秀娟,解慧芳,曹红瑞,杨桂英,谢长荣,梁康迳,孙新立.2016.水稻籼粳亚种分化关联性状的QTL分析[J].福建农林大学学报(自然科学版),45(1):1-7.[Zheng X J,Xie H F,Cao H R,Yang G Y,Xie C R,Liang K J,Sun X L.2016.QTL analysis of the differentiation characteristics of indica and japonica rice and associated traits[J].Journal of Fujian Agriculture and Forestry University(Natural Science Edition),45(1):1-7.]
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