基于SSR标记的同一花序贵州野生白三叶遗传多样性分析
|
摘要
采用简单重复序列(SSR)分子标记技术,对19份分别来源于同一花序的贵州野生白三叶样品进行遗传多样性及亲缘关系研究。结果表明,利用20对引物共扩增出207个条带,其中178个条带具有多态性。20对引物多态性位点的比例为76. 92%～92. 31%,平均多态性为85. 99%。20对引物对19份白三叶材料SSR的PCR扩增条带的多态信息含量为0. 380 6～0. 499 7,平均为0. 463 2。在相似系数为0. 60时,把19份白三叶材料划分为3大类。第1类是W18,最早被分开独立成一类,从SSR标记来看,W18与其余材料的亲缘关系较远。第2类包括W2和W13,表明这2个居群间亲缘关系较近,遗传差异不大。第3类为剩下的16个品种,居群间的遗传关系较复杂。
引文
[1]Meng T T,Ni J,Wang G H.Plant functional traits,environments and ecosystem functioning[J].Journal of Plant Ecology:Chinese Version,2007,31(1):150-165.
[2]Zhang S B,Slik J,Zhang J L,et al.Spatial patterns of wood traits in china are controlled by phylogeny and the environment[J].Global Ecology and Biogeography,2011,20(2):241-250.
[3]张莉,温仲明,苗连朋.延河流域植物功能性状变异来源分析[J].生态学报,2013,33(20):6543-6552.
[4]刘左军,杜国祯,陈家宽.黄帚橐吾花序结构的资源配置与环境的关系[J].植物生态学报,2003,27(3):344-351.
[5]樊宝丽,赵志刚,孟金柳,等.露蕊乌头(Aconitum gymnandrum Maxim)花序内位置依赖的性分配[J].生态学报,2008,28(6):2909-2915.
[6]张鹤山,陈明新,田宏,等.野生红三叶种群表型性状变异研究[J].江西农业大学学报,2012,34(1):44-49.
[7]吴永洁,王元素,李莉.喀斯特地区同一花序白三叶形态多样性研究[J].湖南师范大学自然科学学报,2016,39(5):38-43.
[8]王燕龙,单雷,付春,等.不同SSR标记检测技术及其在花生栽培种遗传多样性分析中的应用[J].植物遗传资源学报,2014,15(1):96-105.
[9]马琳,刘海珍,陆徐忠,等.130份甘蓝型油菜种质分子身份证的构建[J].中国油料作物学报,2013,35(3):231-239.
[10]王峰,官春云.甘蓝型油菜遗传图谱的构建及单株产量构成因素的QTL分析[J].遗传,2010,32(3):271-277.
[11]杨娟,王雯,沈火林.辣椒恢复基因SSR标记定位及分子标记辅助选择育种[J].中国瓜菜,2010,23(5):1-5.
[12]牟彤.白三叶化学诱变后代遗传变异的研究[D].哈尔滨:东北农业大学,2013:28-58.
[13]张婧源.世界范围野生白三叶种质资源的遗传多样性研究[D].雅安:四川农业大学,2013:41-61.
[14]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183.
[15]K9lliker R,Jones E S,Drayton M C,et al.Developmentand characterization of simple sequence repeat(SSR)markers for white clover(Trifolium repens L.)[J].Theoretical and Applied Genetics,2001,102(2):416-424.
[16]车永和,李立会,何蓓如.冰草属植物遗传多样性取样策略基于醇溶蛋白的研究[J].植物遗传资源学报,2004,5(3):216-221.
[17]George J,Dobrowolski M P,Van-Zijil-de-Jong E,et al.Assessment of genetic diversity in cultivars of white clover(Trifolium repens L.)detected by SSR polymorphisms[J].Genome,2006,49(8):919-930.
[18]Roldan-Ruiz I,Dendauw J,Van Bockstaele E,et al.AFLP markers reveal high polymorphic rates in ryegrasses(Lolium spp.)[J].Molecular Breeding,2000,6(2):125-134.
[19]Rohlf F J.NTSYS-PC numerial taxonomy and multivariate analysis system(Version 2.0)[Z].Exter Softwere,1994.
[20]Hamrick J L,Godt M W.Breeding and genetic resources[M].Sunderland:Sinauer,1990.
[21]Slatkin M.Gene flow and geographic structure of natural populations[J].Science,1987,236(4803):787-792.
[22]曲若竹,侯林,吕红丽,等.群体遗传结构中的基因流[J].遗传,2004,26(3):377-382.
[23]Lande R.Genetics and demography in biological conservation[J].Science,1988,241(4872):1455-1460.
[24]Voisey C R,White D R,Wigley P J,et al.Release of transgenic white clover plants expressing Bacillus thuringiensis genes:an ecological perspective[J].Biocontrol Science and Technology,1994,4(4):475-481.
[25]李州,彭燕,张婧源,等.白三叶种质资源形态变异与地理起源的关系[J].草业科学,2012,29(11):1706-1714.
[26]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3):314-331.
[27]李莉,吴永洁,王元素.基于SSR标记的贵州野生白三叶遗传多样性分析[J].种子,2017,36(11):4-9.
[2]Zhang S B,Slik J,Zhang J L,et al.Spatial patterns of wood traits in china are controlled by phylogeny and the environment[J].Global Ecology and Biogeography,2011,20(2):241-250.
[3]张莉,温仲明,苗连朋.延河流域植物功能性状变异来源分析[J].生态学报,2013,33(20):6543-6552.
[4]刘左军,杜国祯,陈家宽.黄帚橐吾花序结构的资源配置与环境的关系[J].植物生态学报,2003,27(3):344-351.
[5]樊宝丽,赵志刚,孟金柳,等.露蕊乌头(Aconitum gymnandrum Maxim)花序内位置依赖的性分配[J].生态学报,2008,28(6):2909-2915.
[6]张鹤山,陈明新,田宏,等.野生红三叶种群表型性状变异研究[J].江西农业大学学报,2012,34(1):44-49.
[7]吴永洁,王元素,李莉.喀斯特地区同一花序白三叶形态多样性研究[J].湖南师范大学自然科学学报,2016,39(5):38-43.
[8]王燕龙,单雷,付春,等.不同SSR标记检测技术及其在花生栽培种遗传多样性分析中的应用[J].植物遗传资源学报,2014,15(1):96-105.
[9]马琳,刘海珍,陆徐忠,等.130份甘蓝型油菜种质分子身份证的构建[J].中国油料作物学报,2013,35(3):231-239.
[10]王峰,官春云.甘蓝型油菜遗传图谱的构建及单株产量构成因素的QTL分析[J].遗传,2010,32(3):271-277.
[11]杨娟,王雯,沈火林.辣椒恢复基因SSR标记定位及分子标记辅助选择育种[J].中国瓜菜,2010,23(5):1-5.
[12]牟彤.白三叶化学诱变后代遗传变异的研究[D].哈尔滨:东北农业大学,2013:28-58.
[13]张婧源.世界范围野生白三叶种质资源的遗传多样性研究[D].雅安:四川农业大学,2013:41-61.
[14]Zietkiewicz E,Rafalski A,Labuda D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-183.
[15]K9lliker R,Jones E S,Drayton M C,et al.Developmentand characterization of simple sequence repeat(SSR)markers for white clover(Trifolium repens L.)[J].Theoretical and Applied Genetics,2001,102(2):416-424.
[16]车永和,李立会,何蓓如.冰草属植物遗传多样性取样策略基于醇溶蛋白的研究[J].植物遗传资源学报,2004,5(3):216-221.
[17]George J,Dobrowolski M P,Van-Zijil-de-Jong E,et al.Assessment of genetic diversity in cultivars of white clover(Trifolium repens L.)detected by SSR polymorphisms[J].Genome,2006,49(8):919-930.
[18]Roldan-Ruiz I,Dendauw J,Van Bockstaele E,et al.AFLP markers reveal high polymorphic rates in ryegrasses(Lolium spp.)[J].Molecular Breeding,2000,6(2):125-134.
[19]Rohlf F J.NTSYS-PC numerial taxonomy and multivariate analysis system(Version 2.0)[Z].Exter Softwere,1994.
[20]Hamrick J L,Godt M W.Breeding and genetic resources[M].Sunderland:Sinauer,1990.
[21]Slatkin M.Gene flow and geographic structure of natural populations[J].Science,1987,236(4803):787-792.
[22]曲若竹,侯林,吕红丽,等.群体遗传结构中的基因流[J].遗传,2004,26(3):377-382.
[23]Lande R.Genetics and demography in biological conservation[J].Science,1988,241(4872):1455-1460.
[24]Voisey C R,White D R,Wigley P J,et al.Release of transgenic white clover plants expressing Bacillus thuringiensis genes:an ecological perspective[J].Biocontrol Science and Technology,1994,4(4):475-481.
[25]李州,彭燕,张婧源,等.白三叶种质资源形态变异与地理起源的关系[J].草业科学,2012,29(11):1706-1714.
[26]Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3):314-331.
[27]李莉,吴永洁,王元素.基于SSR标记的贵州野生白三叶遗传多样性分析[J].种子,2017,36(11):4-9.