糖足颗粒剂外用对神经性糖尿病足大鼠模型的影响
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摘要
目的:观察糖足颗粒剂对神经性糖尿病足动物模型的影响,评价该中药复方的药效动力学。方法:将60只雄性SD大鼠随机分为正常对照组,模型对照组,蒸馏水组,糖足颗粒剂高、中、低剂量组6组,每组各10只。STZ造模成功后,钳夹坐骨神经以制成神经性糖尿病足动物模型。糖足颗粒剂高、中、低剂量组每日给药量为4.08,2.04,1.02μg/g。体质量,用温蒸馏水12.5×10-3 L(37~40℃)溶解后备用。将糖足颗粒剂均匀涂布于大鼠左侧大腿裸露皮肤上,用纱布包裹并用无刺激医用胶布固定,接触1 h后洗净。蒸馏水组同法给予等量等温蒸馏水。正常对照组、模型对照组不予任何处理。每天给药1 h,持续30 d。分别于术后第1,2,4周检测大鼠坐骨神经功能指数(SFI);术后4周,处死大鼠,对比手术侧与正常侧腓肠肌湿重,计算恢复率;光镜下观察术侧腓肠肌细胞生长情况、坐骨神经纵切面轴突再生和髓鞘形成情况,采用软件分析肌细胞的直径、轴突面积、髓鞘面积。结果:大鼠坐骨神经功能指数测定显示,糖足颗粒剂治疗组的SFI分别与蒸馏水组对比,差别无统计学意义(P>0.05);糖足颗粒剂组之间对比,无明显差别(P>0.05)。坐骨神经髓鞘、轴突面积,糖足颗粒剂治疗组与蒸馏水组对比、糖足颗粒剂组之间对比,髓鞘面积总和和轴突面积总和差别均不大(P>0.05)。各组腓肠肌湿重恢复率,糖足颗粒剂治疗组与蒸馏水组对比,差别有统计学意义(P<0.05);糖足颗粒剂组内的恢复率,与糖足颗粒剂高剂量组对比,有统计学意义(P<0.05),与糖足颗粒剂中剂量组对比,差别无统计学意义(P>0.05)。腓肠肌细胞直径,糖足颗粒剂治疗组与蒸馏水组对比,差别有统计学意义(P<0.05);糖足颗粒剂组之间,与糖足颗粒剂高剂量组对比,差别无统计学意义(P>0.05);与糖足颗粒中剂量组对比,糖足颗粒低剂量组肌细胞直径无显著增加,差别无统计学意义(P>0.05)。结论:经过糖足颗粒剂的治疗,神经性糖尿病足大鼠足趾外观有所好转,腓肠肌肌细胞形态恢复明显;但在坐骨神经功能和坐骨神经组织学观察上,糖足颗粒剂治疗组的恢复与对照组无明显差别。
引文
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[2]EDMONDS M,WILSON M,FOSTER A.Diabetic foot ulcers[J].Nurs Stand,1999,14(12):39-45.
[3]中华医学会糖尿病学分会.中国2型糖尿病防治指南(2013版)[J].中国糖尿病杂志,2014,22(8):2-42.
[4]韩锐.糖足煎剂防治糖尿病高危足的远期疗效评价及机理探讨[D].成都:成都中医药大学,2013.
[5]兰兰,惠延年,曾光伟.血管生成素-1对糖尿病大鼠视网膜微血管病变、基质金属蛋白酶和血管内皮生长因子的抑制作用[J].眼科新进展,2012,32(10):926-930.
[6]周婷,孙侃.STZ诱导糖尿病大鼠模型制备及经验[J].农垦医学,2002,24(5):323-324.
[7]HIROSE K,IWAKURA N,ORITA S,et al.Evaluation of behavior and neuropeptide markers of pain in simple,sciatic nerve-pinch painmodel in rats[J].Eur Spine J,2010,19(10):1746-1752.
[8]ZHANG Y,ANDERSON PN,CAMPBELL G,et al.TenascinC expression by neurons and glial cells in the rat spinal cord:changes during postnatal development annd after dorsal root or sciatic nerve injury[J].J Neurocytol,1995,24(8):585-601.
[9]沈宁江,朱家恺.坐骨神经功能指数在神经功能评价中的应用[J].中华显微外科杂志,1993,16(4):284-287.
[10]BAIN JR,MACKINNON SE,HUNTER DA.Functional evaluation of complete sciatic,peroneal,and posterior tibial nerve lesions in the rat[J].Plast Reconstr Surg,1989,83(1):129-138.
[11]TOMLINSON DR,GARDINER NJ.Glucose neurotoxicity[J].Nat Rev Neurosci,2008,9(1):36-45.
[12]MIZUKAMI H,OGASAWARA S,YAMAGISHI S,et al.Methylcobalamin effects on diabetic neuropathy and nerve protein kinase C in rats[J].Eur J Clin Invest,2011,41(4):442-450.