B6-St小鼠与C57BL/6小鼠周围神经再生能力的比较
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摘要
目的通过小鼠坐骨神经损伤模型,比较C57BL/6小鼠和B6-St小鼠(Short tail mice)外周神经再生能力的差异。方法选用C57BL/6小鼠作为对照组,B6-St小鼠为实验组,每组各10只,雌雄各半,夹伤各组小鼠左侧坐骨神经,建立周围神经的损伤模型。手术后8 d、12 d、16 d进行足迹实验,计算坐骨神经功能指数(SFI);手术后21 d,进行电生理检测,记录复合肌动作电位(CMAPs);计算小鼠双侧腓肠肌湿重比;夹伤一侧的腓肠肌制作病理切片并染色,检测肌纤维横切面积(CSA)。结果与C57BL/6小鼠相比,B6-St小鼠术后8d、12d、16dSFI值略有下降(P>0.05);B6-St小鼠坐骨神经夹伤处的复合肌动作电位和C57BL/6小鼠趋于一致(P>0.05);腓肠肌湿重比组间差异无统计学意义(P>0.05);根据病理切片统计分析,两组小鼠肌纤维横截面积CSA相似(P>0.05)。结论 B6-St小鼠与C57BL/6小鼠的周围神经再生能力相类似。
Objective To explore the difference of peripheral nerve regeneration between C57 BL/6 mice and B6-St mice(short tail mice) comparing sciatic nerve injury mice model. Methods Healthy C57 BL/6 mice and B6-St mice were selected for study. Each group was half the male and female. All the mice were treated with left sciatic nerve injury to induce the model mice which were followed up for 8,12 and 16 days after surgery to calculate the sciatic function index(SFI). All mice were subjected to electrophysiological examination 21 days after the operation. The composite muscle motion potentials(CMAPs) were recorded. The bilateral gastrocnemius muscle of each group was used to calculate the wet weight ratio of gastrocnemius muscle. The frozen gastrectomy was performed on the gastrocnemius muscle. The cross-sectional area(CSA) of the muscle fibers was measured by HE staining.Results The SFI values of the two groups were not significantly different on 8, 12 and 16 days after operation(P>0.05). There was no significant difference in the composite muscle between the two groups(P>0.05). There was no significant difference between the two groups in terms of the wet weight of the gastrocnemius muscle. There was no significant difference in the CSA between the two groups. Conclusion The peripheral nerve regeneration ability of B6-St mice is similar to that of C57 BL/6 mice.
Objective To explore the difference of peripheral nerve regeneration between C57 BL/6 mice and B6-St mice(short tail mice) comparing sciatic nerve injury mice model. Methods Healthy C57 BL/6 mice and B6-St mice were selected for study. Each group was half the male and female. All the mice were treated with left sciatic nerve injury to induce the model mice which were followed up for 8,12 and 16 days after surgery to calculate the sciatic function index(SFI). All mice were subjected to electrophysiological examination 21 days after the operation. The composite muscle motion potentials(CMAPs) were recorded. The bilateral gastrocnemius muscle of each group was used to calculate the wet weight ratio of gastrocnemius muscle. The frozen gastrectomy was performed on the gastrocnemius muscle. The cross-sectional area(CSA) of the muscle fibers was measured by HE staining.Results The SFI values of the two groups were not significantly different on 8, 12 and 16 days after operation(P>0.05). There was no significant difference in the composite muscle between the two groups(P>0.05). There was no significant difference between the two groups in terms of the wet weight of the gastrocnemius muscle. There was no significant difference in the CSA between the two groups. Conclusion The peripheral nerve regeneration ability of B6-St mice is similar to that of C57 BL/6 mice.
引文
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[3]王生存,缪进,刘春,等.短尾小鼠脊柱发育形态与病理生理学研究[J].交通医学,2012,26(6):527-529.
[4]蒋茂荣,诸葛晓旻,丁斐.壳寡糖促进小鼠坐骨神经再生的实验研究[J].苏州大学学报:医学版,2010,30(l):4-6.
[5]Wu B,Shao Y,Chen B,et al.Identification of a novel mouse brachyury(T)allele causing a short tail mutation in mice[J].Cell Biochem Biophys,2010,58(3):129-135.
[6]Conlon FL,Smith JC.Interference with Brachyury function inhibits convergent extension,causes apoptosis,and reveals separate requirements in the FGF and activin signalling pathways[J].Dev Biol,1999,213:85-100.
[7]Fekrazad R,Mortezai O,Pedram M,et al.Transected sciatic nerve repair by diode laser protein soldering[J].J Photochem Photobiol B,2017,173:441-447.
[8]Sun X,Zhang Z,Liu N.Absence of split hand in the flail arm variant of ALS[J].Neurophysiol Clin,2016,46(2):149-152.
[9]邵晨昕,吴欣,刘梅,等.Myostatin在小鼠腓肠肌失神经支配萎缩过程中的表达[J].解剖学杂志,2006,29(2):146-149.