头穴丛刺结合跑台训练对坐骨神经损伤大鼠小腿三头肌萎缩调控及Murf 1蛋白表达影响
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摘要
目的:探讨头穴丛刺结合跑台训练对坐骨神经损伤大鼠神经功能恢复、小腿三头肌萎缩,以及肌肉环状指蛋白1(Murf 1)蛋白表达的影响。方法:清洁级大鼠84只,随机分为5组,其中空白组、假手术组分别6只,模型组、跑台组和头穴丛刺结合跑台组,每组24只,并且每组分为7d、14d、21d、28d四个亚组。采用钳夹法造模,假手术组要充分暴露坐骨神经但不钳夹。造模3天后进行干预,头穴丛刺结合跑台组:头穴丛刺+跑台训练,跑台组:跑台训练,其余3组除定时抓取外不做任何干预。各组相应时间点需要测定坐骨神经功能指数(SFI);小腿三头肌湿重、Western Blotting检测泛素蛋白连接酶Murf 1蛋白的表达。结果:(1)小腿三头肌湿重比和坐骨神经功能指数均下降,与模型组比较,头穴丛刺结合跑台组和跑台组均高于模型组(P<0.05);且头穴丛刺结合跑台组高于跑台组(P<0.05);(2)Murf 1蛋白的水平明显增加,与模型组比较,跑台组和头穴丛刺结合跑台组均低于模型组(P<0.05),且头穴丛刺结合跑台组低于跑台组(P<0.05)。结论:头穴丛刺结合跑台训练以及跑台训练均能够提高SFI,下调Murf 1蛋白水平,减少小腿三头肌湿重比下降,延缓肌肉萎缩,提高坐骨神经功能,并且头穴丛刺结合跑台训练优于单纯跑台训练。
Objective: To explore the effects of cluster needling of scalp point combined with treadmill training on neural functional recovery, the atrophy of triceps surae and the expression of muscle ring finger protein 1(Murf 1)protein in rats with sciatic nerve injury.Method: Eighty-four rats of clean grade were randomly divided into 5 groups, including the model group, the treadmill group and the cluster needling of scalp point combined with treadmill group, 24 rats in each group,and each group was divided into 7 d, 14 d and 21 d, 28 d subgroups. We also established the blank group and the control group with 6 rats in each group. The clamps method was used to build model and fully exposed sciatic nerve with no clamps was performed the in control group.After 3 d The intervention was carried out in3 days after model was made: the cluster needling of scalp point combined with treadmill group: the cluster needling of scalp point+ treadmill training, the treadmill group: treadmill training, the other three groups :no intervention except timing grab.The sciatic nerve function index(SFI), the wet weight of triceps surae and the expression of Murf 1 protein were detected at each corresponding time points.Result:(1)The wet weight ratio of triceps surae and the sciatic nerve function index decreased and was higherin the treadmill group and the cluster needling of scalp point combined with treadmill group when compared with the model group(P<0.05). Furthermore the ratio and index were higher in the cluster needling of scalp point combined with treadmill group than that in the treadmill group(P<0.05);(2)The Murf 1 protein level increased significantly. The protein level was lower in the treadmill group and the cluster needling of scalp point combined with treadmill group compared with model group(P<0.05). And the cluster needling of scalp point combined with treadmill group also had the lower Murf 1 protein level than that in the treadmill group(P<0.05).Conclusion:Both the treadmill training and the cluster needling of scalp point combined with treadmill training can improve the SFI, down-regulate Murf 1 protein level, prevent the decrease of wet weight ratio in triceps surae, delay muscle atrophy and enhance the sciatic nerve function. Especially, the cluster needling of scalp point combined with treadmill training has better effect that the treadmill training only.
Objective: To explore the effects of cluster needling of scalp point combined with treadmill training on neural functional recovery, the atrophy of triceps surae and the expression of muscle ring finger protein 1(Murf 1)protein in rats with sciatic nerve injury.Method: Eighty-four rats of clean grade were randomly divided into 5 groups, including the model group, the treadmill group and the cluster needling of scalp point combined with treadmill group, 24 rats in each group,and each group was divided into 7 d, 14 d and 21 d, 28 d subgroups. We also established the blank group and the control group with 6 rats in each group. The clamps method was used to build model and fully exposed sciatic nerve with no clamps was performed the in control group.After 3 d The intervention was carried out in3 days after model was made: the cluster needling of scalp point combined with treadmill group: the cluster needling of scalp point+ treadmill training, the treadmill group: treadmill training, the other three groups :no intervention except timing grab.The sciatic nerve function index(SFI), the wet weight of triceps surae and the expression of Murf 1 protein were detected at each corresponding time points.Result:(1)The wet weight ratio of triceps surae and the sciatic nerve function index decreased and was higherin the treadmill group and the cluster needling of scalp point combined with treadmill group when compared with the model group(P<0.05). Furthermore the ratio and index were higher in the cluster needling of scalp point combined with treadmill group than that in the treadmill group(P<0.05);(2)The Murf 1 protein level increased significantly. The protein level was lower in the treadmill group and the cluster needling of scalp point combined with treadmill group compared with model group(P<0.05). And the cluster needling of scalp point combined with treadmill group also had the lower Murf 1 protein level than that in the treadmill group(P<0.05).Conclusion:Both the treadmill training and the cluster needling of scalp point combined with treadmill training can improve the SFI, down-regulate Murf 1 protein level, prevent the decrease of wet weight ratio in triceps surae, delay muscle atrophy and enhance the sciatic nerve function. Especially, the cluster needling of scalp point combined with treadmill training has better effect that the treadmill training only.
引文
[1]王中鹏,孙佳璐,孙忠人,等.针刺治疗失神经肌萎缩的机制及研究展望[J].针灸临床杂志,2012,(09):73-75.
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[4]郭艳利,马延.超运动与泛素蛋白连接酶MAFbx和MuRFl[J].内江科技,2014,(2):111-112.
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[13]孙忠人,朱路文,于致顺.于氏头穴丛刺针法特色介绍及临床疗效分析[C].北京:中国针灸学会年会,2011.567-570.
[14]邓元江,梁伟雄.针灸治疗阿尔海默病的临床研究进展[J].针灸临床杂志,2007,23(1):59-60.
[15]黄劲柏,潘辉.艾灸百会结合推拿治疗慢性脑供血不足临床观察[J].针灸临床杂志,2011,27(10):31-33.
[16]王艳,裴飞,亢连茹,等.头穴丛刺结合神经松动术治疗下肢周围神经损伤术后的临床观察[J].针灸临床杂志,2015,(5):5-8.
[17]Mocciaro A,Rape M.Emerging regulatory mechanisms in ubiquitin-dependent cell cycle control[J].J Cell Sci,2012,125(Pt 2):255-263.
[18]刘诗濛,董越娟,刘彬.p62与蛋白降解途径的研究进展[J].生理学报,2015,(1):48-58.
[19]Walsh ME,Bhattacharya A,Liu Y,et al.Butyrate prevents muscle atrophy after sciatic nerve crush[J].Muscle Nerve,2015,52(5):859-868.
[20]Bito H,Takemoto-Kimura S.Ca(2+)/CREB/CBP-dependent gene regulation:a shared mechanism critical in long-term synaptic plasticity and neuronal survival[J].Cell Calcium,2003,34(4-5):425-430.
[21]Voss MW,Erickson KI,Prakash RS,et al.Neurobiological markers of exercise-related brain plasticity in older adults[J].Brain Behav Immun,2013,28(3):90-99.
[22]Armada-da-Silva PA,Pereira C,Amado S,et al.Role of physical exercise for improving posttraumatic nerve regeneration[J].Int Rev Neurobiol,2013,109(3):978-1016.
[23]秦春耀.RNA干扰MuRF1基因延缓大鼠失神经骨骼肌萎缩[D].太原:山西医科大学,2015.
[2]吴勃力,于慧敏,杨沈秋.于致顺教授头穴治瘫经验[J].中国针灸1997,(3):153-154.
[3]Boeltz T,Ireland M,Mathis K,et al.Effects of treadmil training on functional recovery following peripheral nerve injury in rats[J].J Neurophysiol,2013,109(11):2645-2657.
[4]郭艳利,马延.超运动与泛素蛋白连接酶MAFbx和MuRFl[J].内江科技,2014,(2):111-112.
[5]Rohde JR,Breitkreutz A,Chenal A,et al.Type III secretion effectors of the IpaH family are E3 ubiquitin ligases[J]Cell Host Microbe,2007,1(1):77-83.
[6]Larsen CN,Finley D.Protein translocation channels in the proteasome and other proteases[J].Cell,1997,91(4):431-434.
[7]Bodine SC,Stitt TN,Gonzalez M,et al.Akt/mTOR pathway is a crucial regulator of skeletal muscle hypertrophy and can prevent muscle atrophy in vivo[J].Nature Cell Biology,2001,3(11):1014-1019.
[8]楚燕飞,朱刚,陈菁,等.联合应用NGF和CNTF对大鼠坐骨神经损伤后功能恢复的影响[J].第三军医大学学报,2002,(4):439-441.
[9]万丽丹,刘厚奇,丁文龙.周围神经损伤后神经组织中Par-3蛋白表达和分布的变化[J].中国组织化学与细胞化学杂志,2010,19(4):335-339.
[10]Bain JR,Mackinnon SE,Hunter DA.Functional evaluation of complete sciatic,peroneal,and posterior tibial nerve lesions in the rat[J].Plast Reconstr Surg,1989,83(1):129-138.
[11]沈宁江,朱家凯.坐骨神经功能指数在神经功能评价中的应用[J].中华显微外科杂志,1993,16(4):284.
[12]王艳,唐强,陈国平.神经松动术结合头穴丛刺与康复训练对臂丛神经损伤后上肢功能的影响[J].中国康复医学杂志,2011,(6):575-576.
[13]孙忠人,朱路文,于致顺.于氏头穴丛刺针法特色介绍及临床疗效分析[C].北京:中国针灸学会年会,2011.567-570.
[14]邓元江,梁伟雄.针灸治疗阿尔海默病的临床研究进展[J].针灸临床杂志,2007,23(1):59-60.
[15]黄劲柏,潘辉.艾灸百会结合推拿治疗慢性脑供血不足临床观察[J].针灸临床杂志,2011,27(10):31-33.
[16]王艳,裴飞,亢连茹,等.头穴丛刺结合神经松动术治疗下肢周围神经损伤术后的临床观察[J].针灸临床杂志,2015,(5):5-8.
[17]Mocciaro A,Rape M.Emerging regulatory mechanisms in ubiquitin-dependent cell cycle control[J].J Cell Sci,2012,125(Pt 2):255-263.
[18]刘诗濛,董越娟,刘彬.p62与蛋白降解途径的研究进展[J].生理学报,2015,(1):48-58.
[19]Walsh ME,Bhattacharya A,Liu Y,et al.Butyrate prevents muscle atrophy after sciatic nerve crush[J].Muscle Nerve,2015,52(5):859-868.
[20]Bito H,Takemoto-Kimura S.Ca(2+)/CREB/CBP-dependent gene regulation:a shared mechanism critical in long-term synaptic plasticity and neuronal survival[J].Cell Calcium,2003,34(4-5):425-430.
[21]Voss MW,Erickson KI,Prakash RS,et al.Neurobiological markers of exercise-related brain plasticity in older adults[J].Brain Behav Immun,2013,28(3):90-99.
[22]Armada-da-Silva PA,Pereira C,Amado S,et al.Role of physical exercise for improving posttraumatic nerve regeneration[J].Int Rev Neurobiol,2013,109(3):978-1016.
[23]秦春耀.RNA干扰MuRF1基因延缓大鼠失神经骨骼肌萎缩[D].太原:山西医科大学,2015.