外施生长素对陆生型空心莲子草不定根生长发育的影响
|
摘要
空心莲子草(Alternanthera philoxeroides)是一种水陆两栖的外来入侵植物,陆生型空心莲子草不定根膨大形成的贮藏根是其越冬、繁殖和扩散的重要基础。研究发现,根系中内源生长素(IAA)在不定根膨大过程中可能发挥了重要的调节作用。本研究利用不同浓度(10、50和100mg·L-1)的外源IAA对盆栽空心莲子草进行叶面喷施处理,探究IAA对于空心莲子草根系生长发育的作用机制。结果表明,外源生长素有利于促进空心莲子草贮藏根的形成,100mg·L-1 IAA处理促进效果最好。其作用机理可能在于IAA影响了空心莲子草根内蔗糖代谢相关酶活性及内源激素含量,促进根内营养物质合成,为根系膨大发育提供充足养分。
Alternanthera philoxeroides is an amphibious alien invasive plant.The storage root formed by adventitious root expansion of terrestrial alligator weed is an important basis for wintering,reproduction,and spread.Studies have found that endogenous auxin(IAA)in the root system may play an important regulatory role in the process of adventitious root expansion.In this study,different concentrations of exogenous IAA(10,50,and 100 mg·L-1)were used to spray potted alligator weed to explore the mechanism of IAA on root growth and development of alligator weed.The results showed that exogenous IAA was beneficial in promoting the formation of storage roots of alligator weed,and the best effect was observed for 100 mg·L-1 IAA treatment.The possible mechanism of action may be that IAA affects the activity of sucrose metabolism-related enzymes and endogenous hormones in the roots of alligator weed,promotes the synthesis of nutrients in roots,and provides sufficient nutrients for root expansion.
Alternanthera philoxeroides is an amphibious alien invasive plant.The storage root formed by adventitious root expansion of terrestrial alligator weed is an important basis for wintering,reproduction,and spread.Studies have found that endogenous auxin(IAA)in the root system may play an important regulatory role in the process of adventitious root expansion.In this study,different concentrations of exogenous IAA(10,50,and 100 mg·L-1)were used to spray potted alligator weed to explore the mechanism of IAA on root growth and development of alligator weed.The results showed that exogenous IAA was beneficial in promoting the formation of storage roots of alligator weed,and the best effect was observed for 100 mg·L-1 IAA treatment.The possible mechanism of action may be that IAA affects the activity of sucrose metabolism-related enzymes and endogenous hormones in the roots of alligator weed,promotes the synthesis of nutrients in roots,and provides sufficient nutrients for root expansion.
引文
[1] WANG B R,LI W G,WANG J B.Genetic diversity of Alternanthera philoxeroides in China.Aquatic Botany,2005,81(3):277-283.
[2] JULIEN M H,BOURNE A S,LOW V H K.Growth of the weed Alternanthera philoxeroides(Martius)Grisebach,(alligator weed)in aquatic and terrestrial habitats in Australia.Plant Protection Quarterly,1992,7:102-108.
[3]李华成,陈中义,周璟.外来入侵植物空心莲子草根系发育调控研究进展.长江大学学报(自然科学版),2014,2(5):66-70.LI H C,CHEN Z Y,ZHOU J.Advances in research on root development regulation of alien invasive plant Alternanthera philoxeroides.Journal of Yangtze University(Nature Science Edition),2014,2(5):66-70.
[4]娄远来.空心莲子草[Alternanthera philoxeroides(Mart.)Griseb.]的生物学、生态学与复配除草剂的增效作用及作用机理研究.南京:南京农业大学博士学位论文,2005.LOU Y L.Study on the biology,ecology of alligator weed[Alternanthera philoxeroides(Mart.)Griseb.]and synergism and mechanism of herbicides.PhD Thesis.Nanjing:Nanjing Agricultural University,2005.
[5] PENG X,LI H,YANG Y,ZHI H,LI C,GUO J.Vegetative propagation capacity of invasive alligator weed through small stolon fragments under different treatments.Scientific Reports,2017,7:43826.
[6]陈中义,李华成.空心莲子草不定根与贮藏根中六种内源激素含量比较.湖北农业科学,2016,55(23):6144-6146.CHEN Z Y,LI H C.Comparison of six endogenous hormone contents in adventitious root and storage root of Alternanthera philoxeroides.Hubei Agricultural Science,2016,55(23):6144-6146.
[7] SHIVANI S,ISHA S,NAVDEEP K,PRATAP K P.Auxin:A master regulator in plant root development.Plant Cell Reports,2013,32(6):741.
[8] HAGEN G.Auxin signal transduction.Essays in Biochemistry,2015,58(3):1-12.
[9]向鹏华,单雪华,胡文玮,颜成生,彭金良,张振华.外源生长素浓度及处理时间对烟苗根系生长发育的影响.湖南农业科学,2014(9):39-42.XIANG P H,SHAN X H,HU W W,YAN C S,PENG J L,ZHANG Z H.Response of root growth and lateral root development of tobacco to exogenous auxin concentration and time gradient.Hunan Agricultural Sciences,2014(9):39-42.
[10]宣伟.血红素加氧酶/一氧化碳信号系统参与生长素调控的黄瓜不定根发生.南京:南京农业大学硕士学位论文,2009.XUAN W.The heme oxygenase/carbon monoxide signaling system is involved in auxin-induced adventitious root development in cucumber.Master Thesis.Nanjing:Nanjing Agricultural University,2009.
[11] CHEN Z Y,TANG W.Molecular mechanisms regulating storage root formation in plants.International Journal of Environmental&Agriculture Research,2017:2454-1850.
[12]孔祥生,易现峰.植物生理学实验技术.北京:中国农业出版社,2008:135-136,160-161.KONG X S,YI X F.Plant Physiology Experimental Technique.Beijing:China Agriculture Press,2008:135-136,160-161.
[13]西北农林科技大学植物生化教研组.植物生理学试验指导.西安:陕西科学技术出版社,1987:114-116.Teaching and Research Group of Plant Biochemistry,Northwest A&F University.Plant Physiology Experiment Guide.Xi’an:Shaanxi Science and Technology Press,1987:114-116.
[14]王惠聪,黄辉白,黄旭明.荔枝果实的糖积累与相关酶活性.园艺学报,2003,30(1):1-5.WANG H C,HUANG H B,HUANG X M.Sugar accumulation and related enzyme activities in the litchi fruit of‘Nuomici’and‘Feizixiao’.Acta Horticulture Sinica,2003,30(1):1-5.
[15] CHEN Q,QI W B,REITER R J,WEI W,WANG B M.Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea.Journal of Plant Physiology,2009,166(3):324-328.
[16] KARIN L,ANNA K H,JOHN C,MASASHI Y,MARK E,JENNIFER N,GRAN S.Sites and regulation of auxin biosynthesis in Arabidopsis roots.Plant Cell,2005,17(4):1090-1104.
[17]刘凤丽,王万鹏,李文滨,刘珊珊,姜振峰.生长素、乙烯和温度对大豆不定根形成的影响.大豆科学,2017,36(4):547-553.LIU F L,WANG W P,LI W B,LIU S S,JIANG Z F.Coordinated effects of IAA and ethylene and temperature on adventitious root organogenesis in soybean hypocotyl cuttings.Soybean Science,2017,36(4):547-553.
[18]孟广目.生长素和细胞分裂素对拟南芥主根细胞分裂和伸长的影响.兰州:兰州大学硕士学位论文,2010.MENG G M.Effects of auxins and cytokinin on Arabidopsis priamary root cell division and elongation.Master Thesis.Lanzhou:Lanzhou University,2010.
[19]曾辉,李超,刘晓,向仰洲.外源激素IAA和6-BA对“薄丰”核桃光合特性的影响.北方园艺,2015(13):31-34.ZENG H,LI C,LIU X,XIANG Y Z.Effect of exogenous hormones IAA and 6-BA on photosynthetic characteristics of‘Bofeng’Walnut(Juglans regia).Northern Horticulture,2015(13):31-34.
[20]伍宝朵,周蓉,陈海峰,沙爱华,单志慧,张晓娟,杨中路,陈水莲,郭丹丹,朱晓玲,张婵娟,周新安.蔗糖载体调控作物“源、库”分配的研究进展.中国农学通报,2012,28(6):19-23.WU B D,ZHOU R,CHEN H F,SHA A H,SHAN Z H,ZHANG X J,YANG Z L,CHEN S L,GUO D D,ZHU X L,ZHANG C J,ZHOU X A.The progress of sucrose transporter in regulating‘Source-sink’distribution.Chinese Agricultural Science Bulletin,2012,28(6):19-23.
[21]张玲,王延秀,高清华,段可.蔗糖转化酶家族基因进化、表达及对草莓果实糖分积累的影响.园艺学报,2017,44(6):1049-1060.ZHANG L,WANG Y X,GAO Q H,DUAN K.The phylogeney,expression patterns of alkaline/neutral Invertase genes(Fa.A/N-Invs)family and their influence on sugar accumulation in strawberry fruit.Acta Horticulturae Sinica,2017,44(6):1049-1060.
[22] Kato T,Kubota S.Properties of invertases in sugar storage tissues of citrus fruit and changes in their activities during maturation.Physiologia Plantarum,1978,42(1):67-72.
[23]王永章,张大鹏.‘红富士’苹果果实蔗糖代谢与酸性转化酶和蔗糖合酶关系的研究.园艺学报,2001,28(3):256-261.WANG Y Z,ZHANG D P.A study on the relationships between acid invertase,sucrose synthase and sucrose metabolism in‘Red Fuji’apple fruit.Acta Horticulturae Sinica,2001,28(3):256-261.
[24] HUBBARD N L,HUBER S C,PHARR D M.Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon(Cucumis melo L.)fruits.Plant Physiology,1989,91(4):1527-1534.
[25] ALONI R,ALONI E,LANGHANS M,ULLRICH C I.Role of cytokinin and auxin in shaping root architecture:Regulating vascular differentiation,lateral root initiation,root apical dominance and root gravitropism.Annals of Botany,2006,97(5):883.
[26]孔晓敏.拟南芥MAPKKKX参与ABA调控主根生长分子机制初探.开封:河南大学硕士学位论文,2012.KONG X M.Primary research on MAPKKKX involved in the primary root growth modulated by ABA in Arabidopsis.Master Thesis.Kaifeng:Henan University,2012.
[27]张立明,王庆美,何钟佩.脱毒和生长调节剂对甘薯内源激素含量及块根产量的影响.中国农业科学,2007,40(1):70-77.ZHANG L M,WANG Q M,HE Z P.Effects of virus-eliminating and plant growth regulators on the endogenous level of hormone and tuber root yield of sweetpotato.Scientia Agricultural Sinica,2007,40(1):70-77.
[28]王庆美,张立明,王振林.甘薯内源激素变化与块根形成膨大的关系.中国农业科学,2005,38(12):2414-2420.WANG Q M,ZHANG L M,WANG Z L.Formation and thickening of tuberous roots in relation to the endogenous hormone concentrations in sweetpotato.Scientia Agricultural Sinica,2005,38(12):2414-2420.
[29]杨永岗,张化生,李亚莉,郁继华.高原夏季胡萝卜肉质根膨大与内源激素含量的关系.中国生态农业学报,2011,19(2):342-346.YANG Y G,ZHANG H S,LI Y L,YU J H.Endogenous hormone content in relation to thickening of carrot fleshy root during summer season in plateau region.Chinese Journal of Eco-Agriculture,2011,19(2):342-346.
[30]杨永岗,张化生,李亚莉,赵祯祥,李晓芳.外源GA3对高原夏季胡萝卜肉质根发育的影响.中国生态农业学报,2014,22(3):308-313.YANG Y G,ZHANG H S,LI Y L,ZHAO Z X,LI X F.Exogenous GA3influence on the development of summer cultivated carrot fleshy root on plateau.Chinese Journal of Eco-Agriculture,2014,22(3):308-313.
[31] CREELMAN R A,MULLET J E.Biosynthesis and action of Jasmonates in plants.Annual Review of Plant Physiology&Plant Molecular Biology,1997,48(48):355-381.
[32]王鹏飞,李鑫宇,李明杰,刘林,王潇然,王丰青,李春奇,陈新建,张重义.地黄块根膨大发生和驱动的组织观察及激素相关基因的调控分析.中国中药杂志,2014,39(17):3245-3253.WANG P F,LI X Y,LI M J,LIU L,WANG X R,WANG F Q,LI C Q,CHEN X J,ZHANG Z Y.Observation of prime position and driving zones in process of tuberous root expanding and expression analysis of phytohormone relative genes in Rehmannia glutinosa.China Journal of Chinese Materia Medica,2014,39(17):3245-3253.
[33]解备涛,王庆美,张海燕,李爱贤,侯夫云,汪宝卿,董顺旭,张立明.植物生长调节剂对甘薯产量和激素含量的影响.华北农学报,2016,31(1):155-161.XIE B T,WANG Q M,ZHANG H Y,LI A X,HOU F Y,WANG B Q,DONG S X,ZHANG L M.The effect of plant growth regulators on the yield and phytohormone concentration in sweetpotato.North China Agricultural Journal,2016,31(1):155-161.
[2] JULIEN M H,BOURNE A S,LOW V H K.Growth of the weed Alternanthera philoxeroides(Martius)Grisebach,(alligator weed)in aquatic and terrestrial habitats in Australia.Plant Protection Quarterly,1992,7:102-108.
[3]李华成,陈中义,周璟.外来入侵植物空心莲子草根系发育调控研究进展.长江大学学报(自然科学版),2014,2(5):66-70.LI H C,CHEN Z Y,ZHOU J.Advances in research on root development regulation of alien invasive plant Alternanthera philoxeroides.Journal of Yangtze University(Nature Science Edition),2014,2(5):66-70.
[4]娄远来.空心莲子草[Alternanthera philoxeroides(Mart.)Griseb.]的生物学、生态学与复配除草剂的增效作用及作用机理研究.南京:南京农业大学博士学位论文,2005.LOU Y L.Study on the biology,ecology of alligator weed[Alternanthera philoxeroides(Mart.)Griseb.]and synergism and mechanism of herbicides.PhD Thesis.Nanjing:Nanjing Agricultural University,2005.
[5] PENG X,LI H,YANG Y,ZHI H,LI C,GUO J.Vegetative propagation capacity of invasive alligator weed through small stolon fragments under different treatments.Scientific Reports,2017,7:43826.
[6]陈中义,李华成.空心莲子草不定根与贮藏根中六种内源激素含量比较.湖北农业科学,2016,55(23):6144-6146.CHEN Z Y,LI H C.Comparison of six endogenous hormone contents in adventitious root and storage root of Alternanthera philoxeroides.Hubei Agricultural Science,2016,55(23):6144-6146.
[7] SHIVANI S,ISHA S,NAVDEEP K,PRATAP K P.Auxin:A master regulator in plant root development.Plant Cell Reports,2013,32(6):741.
[8] HAGEN G.Auxin signal transduction.Essays in Biochemistry,2015,58(3):1-12.
[9]向鹏华,单雪华,胡文玮,颜成生,彭金良,张振华.外源生长素浓度及处理时间对烟苗根系生长发育的影响.湖南农业科学,2014(9):39-42.XIANG P H,SHAN X H,HU W W,YAN C S,PENG J L,ZHANG Z H.Response of root growth and lateral root development of tobacco to exogenous auxin concentration and time gradient.Hunan Agricultural Sciences,2014(9):39-42.
[10]宣伟.血红素加氧酶/一氧化碳信号系统参与生长素调控的黄瓜不定根发生.南京:南京农业大学硕士学位论文,2009.XUAN W.The heme oxygenase/carbon monoxide signaling system is involved in auxin-induced adventitious root development in cucumber.Master Thesis.Nanjing:Nanjing Agricultural University,2009.
[11] CHEN Z Y,TANG W.Molecular mechanisms regulating storage root formation in plants.International Journal of Environmental&Agriculture Research,2017:2454-1850.
[12]孔祥生,易现峰.植物生理学实验技术.北京:中国农业出版社,2008:135-136,160-161.KONG X S,YI X F.Plant Physiology Experimental Technique.Beijing:China Agriculture Press,2008:135-136,160-161.
[13]西北农林科技大学植物生化教研组.植物生理学试验指导.西安:陕西科学技术出版社,1987:114-116.Teaching and Research Group of Plant Biochemistry,Northwest A&F University.Plant Physiology Experiment Guide.Xi’an:Shaanxi Science and Technology Press,1987:114-116.
[14]王惠聪,黄辉白,黄旭明.荔枝果实的糖积累与相关酶活性.园艺学报,2003,30(1):1-5.WANG H C,HUANG H B,HUANG X M.Sugar accumulation and related enzyme activities in the litchi fruit of‘Nuomici’and‘Feizixiao’.Acta Horticulture Sinica,2003,30(1):1-5.
[15] CHEN Q,QI W B,REITER R J,WEI W,WANG B M.Exogenously applied melatonin stimulates root growth and raises endogenous indoleacetic acid in roots of etiolated seedlings of Brassica juncea.Journal of Plant Physiology,2009,166(3):324-328.
[16] KARIN L,ANNA K H,JOHN C,MASASHI Y,MARK E,JENNIFER N,GRAN S.Sites and regulation of auxin biosynthesis in Arabidopsis roots.Plant Cell,2005,17(4):1090-1104.
[17]刘凤丽,王万鹏,李文滨,刘珊珊,姜振峰.生长素、乙烯和温度对大豆不定根形成的影响.大豆科学,2017,36(4):547-553.LIU F L,WANG W P,LI W B,LIU S S,JIANG Z F.Coordinated effects of IAA and ethylene and temperature on adventitious root organogenesis in soybean hypocotyl cuttings.Soybean Science,2017,36(4):547-553.
[18]孟广目.生长素和细胞分裂素对拟南芥主根细胞分裂和伸长的影响.兰州:兰州大学硕士学位论文,2010.MENG G M.Effects of auxins and cytokinin on Arabidopsis priamary root cell division and elongation.Master Thesis.Lanzhou:Lanzhou University,2010.
[19]曾辉,李超,刘晓,向仰洲.外源激素IAA和6-BA对“薄丰”核桃光合特性的影响.北方园艺,2015(13):31-34.ZENG H,LI C,LIU X,XIANG Y Z.Effect of exogenous hormones IAA and 6-BA on photosynthetic characteristics of‘Bofeng’Walnut(Juglans regia).Northern Horticulture,2015(13):31-34.
[20]伍宝朵,周蓉,陈海峰,沙爱华,单志慧,张晓娟,杨中路,陈水莲,郭丹丹,朱晓玲,张婵娟,周新安.蔗糖载体调控作物“源、库”分配的研究进展.中国农学通报,2012,28(6):19-23.WU B D,ZHOU R,CHEN H F,SHA A H,SHAN Z H,ZHANG X J,YANG Z L,CHEN S L,GUO D D,ZHU X L,ZHANG C J,ZHOU X A.The progress of sucrose transporter in regulating‘Source-sink’distribution.Chinese Agricultural Science Bulletin,2012,28(6):19-23.
[21]张玲,王延秀,高清华,段可.蔗糖转化酶家族基因进化、表达及对草莓果实糖分积累的影响.园艺学报,2017,44(6):1049-1060.ZHANG L,WANG Y X,GAO Q H,DUAN K.The phylogeney,expression patterns of alkaline/neutral Invertase genes(Fa.A/N-Invs)family and their influence on sugar accumulation in strawberry fruit.Acta Horticulturae Sinica,2017,44(6):1049-1060.
[22] Kato T,Kubota S.Properties of invertases in sugar storage tissues of citrus fruit and changes in their activities during maturation.Physiologia Plantarum,1978,42(1):67-72.
[23]王永章,张大鹏.‘红富士’苹果果实蔗糖代谢与酸性转化酶和蔗糖合酶关系的研究.园艺学报,2001,28(3):256-261.WANG Y Z,ZHANG D P.A study on the relationships between acid invertase,sucrose synthase and sucrose metabolism in‘Red Fuji’apple fruit.Acta Horticulturae Sinica,2001,28(3):256-261.
[24] HUBBARD N L,HUBER S C,PHARR D M.Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon(Cucumis melo L.)fruits.Plant Physiology,1989,91(4):1527-1534.
[25] ALONI R,ALONI E,LANGHANS M,ULLRICH C I.Role of cytokinin and auxin in shaping root architecture:Regulating vascular differentiation,lateral root initiation,root apical dominance and root gravitropism.Annals of Botany,2006,97(5):883.
[26]孔晓敏.拟南芥MAPKKKX参与ABA调控主根生长分子机制初探.开封:河南大学硕士学位论文,2012.KONG X M.Primary research on MAPKKKX involved in the primary root growth modulated by ABA in Arabidopsis.Master Thesis.Kaifeng:Henan University,2012.
[27]张立明,王庆美,何钟佩.脱毒和生长调节剂对甘薯内源激素含量及块根产量的影响.中国农业科学,2007,40(1):70-77.ZHANG L M,WANG Q M,HE Z P.Effects of virus-eliminating and plant growth regulators on the endogenous level of hormone and tuber root yield of sweetpotato.Scientia Agricultural Sinica,2007,40(1):70-77.
[28]王庆美,张立明,王振林.甘薯内源激素变化与块根形成膨大的关系.中国农业科学,2005,38(12):2414-2420.WANG Q M,ZHANG L M,WANG Z L.Formation and thickening of tuberous roots in relation to the endogenous hormone concentrations in sweetpotato.Scientia Agricultural Sinica,2005,38(12):2414-2420.
[29]杨永岗,张化生,李亚莉,郁继华.高原夏季胡萝卜肉质根膨大与内源激素含量的关系.中国生态农业学报,2011,19(2):342-346.YANG Y G,ZHANG H S,LI Y L,YU J H.Endogenous hormone content in relation to thickening of carrot fleshy root during summer season in plateau region.Chinese Journal of Eco-Agriculture,2011,19(2):342-346.
[30]杨永岗,张化生,李亚莉,赵祯祥,李晓芳.外源GA3对高原夏季胡萝卜肉质根发育的影响.中国生态农业学报,2014,22(3):308-313.YANG Y G,ZHANG H S,LI Y L,ZHAO Z X,LI X F.Exogenous GA3influence on the development of summer cultivated carrot fleshy root on plateau.Chinese Journal of Eco-Agriculture,2014,22(3):308-313.
[31] CREELMAN R A,MULLET J E.Biosynthesis and action of Jasmonates in plants.Annual Review of Plant Physiology&Plant Molecular Biology,1997,48(48):355-381.
[32]王鹏飞,李鑫宇,李明杰,刘林,王潇然,王丰青,李春奇,陈新建,张重义.地黄块根膨大发生和驱动的组织观察及激素相关基因的调控分析.中国中药杂志,2014,39(17):3245-3253.WANG P F,LI X Y,LI M J,LIU L,WANG X R,WANG F Q,LI C Q,CHEN X J,ZHANG Z Y.Observation of prime position and driving zones in process of tuberous root expanding and expression analysis of phytohormone relative genes in Rehmannia glutinosa.China Journal of Chinese Materia Medica,2014,39(17):3245-3253.
[33]解备涛,王庆美,张海燕,李爱贤,侯夫云,汪宝卿,董顺旭,张立明.植物生长调节剂对甘薯产量和激素含量的影响.华北农学报,2016,31(1):155-161.XIE B T,WANG Q M,ZHANG H Y,LI A X,HOU F Y,WANG B Q,DONG S X,ZHANG L M.The effect of plant growth regulators on the yield and phytohormone concentration in sweetpotato.North China Agricultural Journal,2016,31(1):155-161.