城市污泥中蛋白质资源化的研究进展
|
摘要
资源化是城市污泥处理处置的必然趋势,回收利用污泥中丰富的蛋白质用作动物饲料添加剂、发泡剂原料和肥料等是一个新的研究方向。蛋白质资源化包括污泥水解提取蛋白质、蛋白质的纯化分离和污泥蛋白质的开发应用3个步骤。其中,提取蛋白质的方法中热-碱法、热-酸法、超声-碱法和超声-酶法的蛋白质提取率在65%以上。蛋白质纯化的过程中循环水解和膜技术能进一步提高提取液中蛋白质的浓度,盐析沉淀法和等电点沉淀法可回收其中85%以上的蛋白质。用作动物饲料添加剂和肥料是提取纯化出的蛋白质最有前景的应用方式。最后对城市污泥中蛋白质资源化的研究方向提出了建议与展望。
Reutilization is an inevitable trend in the treatment and disposal of municipal sludge. The reutilization of abundant protein in sludge as animal feed additive,raw materials of foaming agent,fertilizer,etc,is a new direction. The reutilization of protein includes three steps: sludge hydrolysis and protein extraction,protein purification and separation,development and application of sludge protein. The extraction rate of protein is more than 65% by the means of thermo-alkaline method,thermoacid method,ultrasonic-alkali method or ultrasonic-enzymatic method. The cyclic hydrolysis and membrane treatment in the purification of protein can improve the concentration of protein in the extracting solution. Salting out precipitation and isoelectric precipitation can recover more than 85% of the total protein. The application of the extracted and purified protein as feed addictive and fertilizer is the most promising method. Finally,some suggestions and prospects on the development of protein reutilization are proposed.
Reutilization is an inevitable trend in the treatment and disposal of municipal sludge. The reutilization of abundant protein in sludge as animal feed additive,raw materials of foaming agent,fertilizer,etc,is a new direction. The reutilization of protein includes three steps: sludge hydrolysis and protein extraction,protein purification and separation,development and application of sludge protein. The extraction rate of protein is more than 65% by the means of thermo-alkaline method,thermoacid method,ultrasonic-alkali method or ultrasonic-enzymatic method. The cyclic hydrolysis and membrane treatment in the purification of protein can improve the concentration of protein in the extracting solution. Salting out precipitation and isoelectric precipitation can recover more than 85% of the total protein. The application of the extracted and purified protein as feed addictive and fertilizer is the most promising method. Finally,some suggestions and prospects on the development of protein reutilization are proposed.
引文
[1]戴晓虎.我国城镇污泥处理处置现状及思考[J].给水排水,2012,38(2):1-5.
[2] Zhang P Y, Zhang G M, Wang W. Ultrasonic treatment of biological sludge:floc disintegration,cell lysis and inactivation[J]. Bioresource Technology,2007,98(1):207-210.
[3] Raszka A,Chorvatova M,Wanner Jiri. The role and significance of extracellular polymers in activated sludge. Part I:literature review[J]. Clean Soil Air Water,2006,34(5):411-424.
[4]陈玉辉,华佳,霍苗,等.酸循环水解制备剩余污泥水解蛋白质的试验研究[J].湖北大学学报(自然科学版),2006,28(4):417-419.
[5]赵顺顺,孟范平.酸水解法提取剩余污泥蛋白质的条件优化[J].环境科学研究,2008,21(3):180-184.
[6]薛咏海,左健,崔静,等.污泥水解处理及微生物蛋白资源化利用[J].中国给水排水,2014,30(24):102-104.
[7]刘玉蕾.城市污水厂污泥回收蛋白质的技术研究[D].哈尔滨:哈尔滨工业大学,2012.
[8] Xiao K K,Chen Y,Jiang X,et al. Comparision of different treatment methods for protein solubilisation from waste activated sludge[J]. Water Research,2017,122:492-502.
[9] Manuel Garcia,Jose L Urrea,Sergio Collado,et al. Protein recovery from solubilized sludge by hydrothermal treatments[J].Waste Management,2017,67:278-287.
[10]肖本益,刘俊新.不同预处理方法对剩余污泥性质的影响研究[J].环境科学,2008,29(2):327-331.
[11]马欣.生物酶法提取剩余污泥蛋白质的研究[D].天津:天津理工大学,2016.
[12]赵向阳,王茜,陈晓刚,等.响应面法酶解剩余活性污泥中微生物蛋白质研究[J].华南师范大学学报(自然科学版),2014,46(5):85-91.
[13]章文锋,苏瑞景,李登新.酶法水解提取剩余活性污泥蛋白质条件实验[J].环境科学与技术,2012,35(9):7-10.
[14]崔静,董岸杰,张卫江,等.热碱水解提取污泥蛋白质的实验研究[J].环境工程学报,2009,3(10):1889-1892.
[15] Xiang Y L,Xiang Y K,Wang L P. Kinetics of activated sludge protein extraction by thermal alkaline treatment[J]. Journal of Environmental Chemical Engineering,2017,5(6):5352-5357.
[16]李政.化学水解法提取污泥蛋白质及其脱水性能研究[D].郑州:郑州大学,2017.
[17]翟世民,肖本益,郭雪松,等.响应面优化热碱回收污泥蛋白质条件的研究[J].给水排水,2016,52(增刊1):113-117.
[18] Liu Y S,Kong S F,Li Y Q,et al. Novel technology for sewage sludge utilization:preparation of amino acids chelated trace elements fertilizer[J]. Journal of hazardous Materials,2009,171:1159-1167.
[19]邵金星,吕斌,王弘宇.酸和热解耦合法提取剩余污泥蛋白质的研究[J].武汉纺织大学学报,2015,28(3):76-79.
[20]陈伟华,李萍,陈广大,等.从干剩余污泥中提取蛋白质的试验研究[J].地球与环境,2011,39(3):435-439.
[21]相玉琳,张卫江,徐姣,等.超声波辐射强化污泥蛋白质的提取[J].化学工程,2011,39(3):63-66.
[22] Wei L L,Wang K,Kong X J,et al. Application of ultrasonication, acid precipitation and membrane filtration for corecovery of protein and humic acid from sewage sludge[J].Frontiers of Environmental Science and Engineering,2016,10(2):327-335.
[23]陆钧皓,李登新.超声波与复合酶联合提取剩余污泥蛋白的研究[J].环境科学与技术,2015,38(10):53-58.
[24]苗丛瑶.超声波强化酶水解提取污泥蛋白质研究[D].郑州:郑州大学,2017.
[25]赵顺顺,孟范平,王震宇.碱水解法提取剩余污泥蛋白质的条件优化[J].城市环境与城市生态,2008,21(5):17-20.
[26]秦晓,刘勇.污泥热碱协同溶胞下蛋白质溶出动力学模型[J].化学反应工程与工艺,2011,27(5):425-431.
[27] Tyagi V K,Lo S L. Application of physico-chemistry pretreatment methods to enhance the sludge disintegration and subsequent anaerobic digestion:an up to date review[J]. Environmental Science and Biotechnology,2011,10:215-242.
[28]秦晓,刘勇.盐酸法提取污泥中蛋白质过程的研究[J].环境保护科学,2012,38(2):48-52.
[29] Li C L,Liu G F,Jin R F,et al. Kinetics model for combined(alkaline+ultrasonic)sludge disintegration[J]. Bioresource Technology,2010,101(22):8555-8557.
[30]罗琨,杨麒,李小明,等.外加酶强化剩余污泥水解的研究[J].环境科学,2010,31(3):763-767.
[31]陈小粉,李小明,杨麒,等.淀粉酶促进剩余污泥热水解的研究[J].中国环境科学,2011,31(3):396-401.
[32]李萍,李登新,苏瑞景,等. 2种处理方法水解剩余污泥蛋白质的研究[J].环境工程学报,2011,5(12):2859-2863.
[33]李萍,雷莹,李登新.超声波协同生物法提取剩余污泥中蛋白质的研究[J].中国给水排水,2014,30(1):22-25.
[34] Su R J,Hussain A,Guo J,et al. Animal feeds extracted from excess sludge by enzyme,acid and base hydrolysis processes[J].American Chemical Society Sustainable Chemistry and Engineering,2015,3(9):2084-2091.
[35]徐林.膜技术处理活性污泥水解蛋白液实验研究[D].天津:天津大学,2008.
[36]庞金钊,张淑玲,刘忠,等.用超滤膜法浓缩剩余污泥中蛋白提取液的实验研究[J].天津工业大学学报,2009,28(6):7-10.
[37]郝少莉,仇农学.沉淀分离技术在蛋白质处理方面的应用[J].粮食与食品工业,2007,(1):20-22.
[38]吕薇,蒋剑春,徐俊明.蛋白质提取及分离纯化研究进展[J].精细石油化工进展,2010,11(11):52-58.
[39]施正华,李秀芬,宋小莉,等.采用等电点沉淀法回收市政污泥水解液中的蛋白质[J].环境工程学报,2016,10(10):5919-5923.
[40]应诚威,诸玉辉,修光利.剩余活性污泥中微生物蛋白质的提取工艺研究[J].环境科学与管理,2011,36(8):73-76.
[41]弓凤莲,赵丹贤,应成辉.活性污泥微生物蛋白质提取效果的影响因素研究[J].中国给水排水,2014,30(21):124-127.
[42]王辽卫. 2017年我国饲料原料市场回顾与展望[J].猪业科学,2018,35(2):49-51.
[43]赵顺顺,孟范平.剩余污泥蛋白质作为动物饲料添加剂的营养性和安全性分析[J].中国饲料,2008,(15):35-38.
[44]许丹丹,何艮,周慧慧,等.玉米蛋白粉部分替代鱼粉对大菱鲆幼鱼的生长性能和游离氨基酸代谢的影响[J].中国海洋大学学报(自然科学版),2016,46(2):26-34.
[45]陈宇,柏凡,方思敏,等.肉粉的营养价值研究与安全性评价[J].中国饲料,2017(7):35-39.
[46]张婵娟,王建萍,丁雪梅,等.不同来源豆粕对大恒肉鸡的能量和氨基酸营养价值评定[J].动物营养学报,2018,30(4):1320-1332.
[47]郭广慧,陈同斌,杨军,等.中国城市污泥重金属区域分布特征及变化趋势[J].环境科学学报,2014,34(10):2455-2461.
[48] Hwang J,Zhang L,Seo S,et al. Protein recovery from excess sludge for its use as animal feed[J]. Bioresource Technology,2008,99:8949-8954.
[49]李亚东,李海波.利用剩余活性污泥水解蛋白质制备蛋白质泡沫灭火剂的研究[J].湖北大学学报(自然科学版),2005,(1):91-93.
[50]汪常青,梁浩,李亚东,等.利用剩余污泥制备泡沫灭火剂的试验研究[J].中国给水排水,2006,(9):38-42.
[51]李军伟.活性污泥蛋白质混凝土发泡剂的泡沫稳定性研究[J].新型建筑材料,2010,37(12):63-66.
[52]马小莉,相玉琳.改性污泥制备泡沫混凝土的可行性研究[J].化工进展,2016,35(9):2997-3001.
[53]倪红,陈婷,李亚东.高稳定性污泥蛋白发泡剂的发泡特性及应用研究[J].环境工程学报,2009,3(12):2254-2260.
[54]陈婷,霍苗,李义波,等.不同稳定剂对污泥蛋白发泡特性的影响研究[J].环境工程学报,2010,4(5):1181-1185.
[55]岳秀伟,张卫江,徐娇,等.污泥微生物蛋白发泡剂的改性[J].化工进展,2011,30(6):1316-1319.
[56] Xiang Y L,Zhang W J,Zheng H. Decolorization kinetics of sludge protein solution by60Coγ-ray irradiation/H2O2Oxidation[J].Transactions of Tianjin University,2011,17(1):45-50.
[57]相玉琳,张卫江.污泥蛋白液的除臭研究[J].化学工程,2012,40(2):7-11.
[58] Belhaj D,Elloumi N,Jerbi B,et al. Effects of sewage sludge fertilizer on heavy metal accumulation and consequent responses of sunflower(Helianthus annuus)[J]. Environmental Science and Pollution Research,2016,23(20):20168-20177.
[59]史舟芳,刘祎,刘晓娟,等.利用剩余活性污泥制备氨基酸叶面肥[J].山东化工,2015,44(10):189-192,199.
[60]汤秋云,高琪,李思彤,等.污泥蛋白肽对土壤微生态及植物生长调控[J].环境工程学报,2015,9(11):5611-5616.
[61]李祥祥,孟祥美,万月亮,等.生物污泥蛋白质作为增强剂在瓦楞原纸中的应用[J].天津造纸,2016,38(4):26-30.
[62] Pervaiz M,Sain M. Protein extraction from secondary sludge of paper mill wastewater and its utilization as a wood adhesive[J].Bioresources,2011,6(2):961-970.
[2] Zhang P Y, Zhang G M, Wang W. Ultrasonic treatment of biological sludge:floc disintegration,cell lysis and inactivation[J]. Bioresource Technology,2007,98(1):207-210.
[3] Raszka A,Chorvatova M,Wanner Jiri. The role and significance of extracellular polymers in activated sludge. Part I:literature review[J]. Clean Soil Air Water,2006,34(5):411-424.
[4]陈玉辉,华佳,霍苗,等.酸循环水解制备剩余污泥水解蛋白质的试验研究[J].湖北大学学报(自然科学版),2006,28(4):417-419.
[5]赵顺顺,孟范平.酸水解法提取剩余污泥蛋白质的条件优化[J].环境科学研究,2008,21(3):180-184.
[6]薛咏海,左健,崔静,等.污泥水解处理及微生物蛋白资源化利用[J].中国给水排水,2014,30(24):102-104.
[7]刘玉蕾.城市污水厂污泥回收蛋白质的技术研究[D].哈尔滨:哈尔滨工业大学,2012.
[8] Xiao K K,Chen Y,Jiang X,et al. Comparision of different treatment methods for protein solubilisation from waste activated sludge[J]. Water Research,2017,122:492-502.
[9] Manuel Garcia,Jose L Urrea,Sergio Collado,et al. Protein recovery from solubilized sludge by hydrothermal treatments[J].Waste Management,2017,67:278-287.
[10]肖本益,刘俊新.不同预处理方法对剩余污泥性质的影响研究[J].环境科学,2008,29(2):327-331.
[11]马欣.生物酶法提取剩余污泥蛋白质的研究[D].天津:天津理工大学,2016.
[12]赵向阳,王茜,陈晓刚,等.响应面法酶解剩余活性污泥中微生物蛋白质研究[J].华南师范大学学报(自然科学版),2014,46(5):85-91.
[13]章文锋,苏瑞景,李登新.酶法水解提取剩余活性污泥蛋白质条件实验[J].环境科学与技术,2012,35(9):7-10.
[14]崔静,董岸杰,张卫江,等.热碱水解提取污泥蛋白质的实验研究[J].环境工程学报,2009,3(10):1889-1892.
[15] Xiang Y L,Xiang Y K,Wang L P. Kinetics of activated sludge protein extraction by thermal alkaline treatment[J]. Journal of Environmental Chemical Engineering,2017,5(6):5352-5357.
[16]李政.化学水解法提取污泥蛋白质及其脱水性能研究[D].郑州:郑州大学,2017.
[17]翟世民,肖本益,郭雪松,等.响应面优化热碱回收污泥蛋白质条件的研究[J].给水排水,2016,52(增刊1):113-117.
[18] Liu Y S,Kong S F,Li Y Q,et al. Novel technology for sewage sludge utilization:preparation of amino acids chelated trace elements fertilizer[J]. Journal of hazardous Materials,2009,171:1159-1167.
[19]邵金星,吕斌,王弘宇.酸和热解耦合法提取剩余污泥蛋白质的研究[J].武汉纺织大学学报,2015,28(3):76-79.
[20]陈伟华,李萍,陈广大,等.从干剩余污泥中提取蛋白质的试验研究[J].地球与环境,2011,39(3):435-439.
[21]相玉琳,张卫江,徐姣,等.超声波辐射强化污泥蛋白质的提取[J].化学工程,2011,39(3):63-66.
[22] Wei L L,Wang K,Kong X J,et al. Application of ultrasonication, acid precipitation and membrane filtration for corecovery of protein and humic acid from sewage sludge[J].Frontiers of Environmental Science and Engineering,2016,10(2):327-335.
[23]陆钧皓,李登新.超声波与复合酶联合提取剩余污泥蛋白的研究[J].环境科学与技术,2015,38(10):53-58.
[24]苗丛瑶.超声波强化酶水解提取污泥蛋白质研究[D].郑州:郑州大学,2017.
[25]赵顺顺,孟范平,王震宇.碱水解法提取剩余污泥蛋白质的条件优化[J].城市环境与城市生态,2008,21(5):17-20.
[26]秦晓,刘勇.污泥热碱协同溶胞下蛋白质溶出动力学模型[J].化学反应工程与工艺,2011,27(5):425-431.
[27] Tyagi V K,Lo S L. Application of physico-chemistry pretreatment methods to enhance the sludge disintegration and subsequent anaerobic digestion:an up to date review[J]. Environmental Science and Biotechnology,2011,10:215-242.
[28]秦晓,刘勇.盐酸法提取污泥中蛋白质过程的研究[J].环境保护科学,2012,38(2):48-52.
[29] Li C L,Liu G F,Jin R F,et al. Kinetics model for combined(alkaline+ultrasonic)sludge disintegration[J]. Bioresource Technology,2010,101(22):8555-8557.
[30]罗琨,杨麒,李小明,等.外加酶强化剩余污泥水解的研究[J].环境科学,2010,31(3):763-767.
[31]陈小粉,李小明,杨麒,等.淀粉酶促进剩余污泥热水解的研究[J].中国环境科学,2011,31(3):396-401.
[32]李萍,李登新,苏瑞景,等. 2种处理方法水解剩余污泥蛋白质的研究[J].环境工程学报,2011,5(12):2859-2863.
[33]李萍,雷莹,李登新.超声波协同生物法提取剩余污泥中蛋白质的研究[J].中国给水排水,2014,30(1):22-25.
[34] Su R J,Hussain A,Guo J,et al. Animal feeds extracted from excess sludge by enzyme,acid and base hydrolysis processes[J].American Chemical Society Sustainable Chemistry and Engineering,2015,3(9):2084-2091.
[35]徐林.膜技术处理活性污泥水解蛋白液实验研究[D].天津:天津大学,2008.
[36]庞金钊,张淑玲,刘忠,等.用超滤膜法浓缩剩余污泥中蛋白提取液的实验研究[J].天津工业大学学报,2009,28(6):7-10.
[37]郝少莉,仇农学.沉淀分离技术在蛋白质处理方面的应用[J].粮食与食品工业,2007,(1):20-22.
[38]吕薇,蒋剑春,徐俊明.蛋白质提取及分离纯化研究进展[J].精细石油化工进展,2010,11(11):52-58.
[39]施正华,李秀芬,宋小莉,等.采用等电点沉淀法回收市政污泥水解液中的蛋白质[J].环境工程学报,2016,10(10):5919-5923.
[40]应诚威,诸玉辉,修光利.剩余活性污泥中微生物蛋白质的提取工艺研究[J].环境科学与管理,2011,36(8):73-76.
[41]弓凤莲,赵丹贤,应成辉.活性污泥微生物蛋白质提取效果的影响因素研究[J].中国给水排水,2014,30(21):124-127.
[42]王辽卫. 2017年我国饲料原料市场回顾与展望[J].猪业科学,2018,35(2):49-51.
[43]赵顺顺,孟范平.剩余污泥蛋白质作为动物饲料添加剂的营养性和安全性分析[J].中国饲料,2008,(15):35-38.
[44]许丹丹,何艮,周慧慧,等.玉米蛋白粉部分替代鱼粉对大菱鲆幼鱼的生长性能和游离氨基酸代谢的影响[J].中国海洋大学学报(自然科学版),2016,46(2):26-34.
[45]陈宇,柏凡,方思敏,等.肉粉的营养价值研究与安全性评价[J].中国饲料,2017(7):35-39.
[46]张婵娟,王建萍,丁雪梅,等.不同来源豆粕对大恒肉鸡的能量和氨基酸营养价值评定[J].动物营养学报,2018,30(4):1320-1332.
[47]郭广慧,陈同斌,杨军,等.中国城市污泥重金属区域分布特征及变化趋势[J].环境科学学报,2014,34(10):2455-2461.
[48] Hwang J,Zhang L,Seo S,et al. Protein recovery from excess sludge for its use as animal feed[J]. Bioresource Technology,2008,99:8949-8954.
[49]李亚东,李海波.利用剩余活性污泥水解蛋白质制备蛋白质泡沫灭火剂的研究[J].湖北大学学报(自然科学版),2005,(1):91-93.
[50]汪常青,梁浩,李亚东,等.利用剩余污泥制备泡沫灭火剂的试验研究[J].中国给水排水,2006,(9):38-42.
[51]李军伟.活性污泥蛋白质混凝土发泡剂的泡沫稳定性研究[J].新型建筑材料,2010,37(12):63-66.
[52]马小莉,相玉琳.改性污泥制备泡沫混凝土的可行性研究[J].化工进展,2016,35(9):2997-3001.
[53]倪红,陈婷,李亚东.高稳定性污泥蛋白发泡剂的发泡特性及应用研究[J].环境工程学报,2009,3(12):2254-2260.
[54]陈婷,霍苗,李义波,等.不同稳定剂对污泥蛋白发泡特性的影响研究[J].环境工程学报,2010,4(5):1181-1185.
[55]岳秀伟,张卫江,徐娇,等.污泥微生物蛋白发泡剂的改性[J].化工进展,2011,30(6):1316-1319.
[56] Xiang Y L,Zhang W J,Zheng H. Decolorization kinetics of sludge protein solution by60Coγ-ray irradiation/H2O2Oxidation[J].Transactions of Tianjin University,2011,17(1):45-50.
[57]相玉琳,张卫江.污泥蛋白液的除臭研究[J].化学工程,2012,40(2):7-11.
[58] Belhaj D,Elloumi N,Jerbi B,et al. Effects of sewage sludge fertilizer on heavy metal accumulation and consequent responses of sunflower(Helianthus annuus)[J]. Environmental Science and Pollution Research,2016,23(20):20168-20177.
[59]史舟芳,刘祎,刘晓娟,等.利用剩余活性污泥制备氨基酸叶面肥[J].山东化工,2015,44(10):189-192,199.
[60]汤秋云,高琪,李思彤,等.污泥蛋白肽对土壤微生态及植物生长调控[J].环境工程学报,2015,9(11):5611-5616.
[61]李祥祥,孟祥美,万月亮,等.生物污泥蛋白质作为增强剂在瓦楞原纸中的应用[J].天津造纸,2016,38(4):26-30.
[62] Pervaiz M,Sain M. Protein extraction from secondary sludge of paper mill wastewater and its utilization as a wood adhesive[J].Bioresources,2011,6(2):961-970.