血清长链非编码RNA ANRIL、基质金属蛋白酶-2与血清淀粉样蛋白A在食管癌中的表达及临床意义
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摘要
目的分析血清的细胞周期激酶抑制因子4b(INK4b)位点的反义非编码RNA(antisense non-coding RNA in the INK4 Locus,lncRNA ANRIL)、基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)与血清淀粉样蛋白A(serum amyloid protein A,SAA)在食管癌中的表达及临床意义。方法食管癌病人80例,同期体检健康人群100例。采用实时定量聚合酶链反应(real-time PCR)法检测血清lncRNA ANRIL;采用酶联免疫吸附法(ELISA)和酶法分别检测MMP-2与SAA水平。结果食管癌病人lncRNA ANRIL、MMP-2与SAA水平高于对照组,差异有统计学意义(P<0.05)。食管癌病人lncRNA ANRIL、MMP-2和SAA水平与肿瘤TNM分期、组织学类型、肿瘤分化程度等显著相关(P<0.05),且在中晚期食管癌、食管腺鳞癌及低分化食管癌病人中的增高最为显著。lncRNA ANRIL联合MMP-2与SAA在区分食管癌与对照的曲线下面积(area under the curve,AUC)为0.927(95%CI:0.900～0.954,P<0.05),灵敏度为80.1%,特异度为90.4%。多因素分析显示,高lncRNA ANRIL、MMP-2和SAA水平是食管癌发病的独立危险因素。结论 lncRNA ANRIL、MMP-2和SAA对食管癌具有诊断价值,可作为食管癌发病风险预测的参考指标。
Objective To explore the diagnostic values of long non-coding RNA(lncRNA)ANRIL,matrix metalloproteinase-2(MMP-2)and serum amyloid protein A(SAA)for esophageal cancer,and the relationship between the three and the clinicopathological features of esophageal cancer.Methods 80 cases of esophageal cancer diagnosed in our hospital from January 2016 to December 2017 were collected,and 100 healthy people were recruited in our hospital during the same period.The levels of lncRNA ANRIL in the peripheral blood of subjects were detected by real-time quantitative PCR(RT-PCR).Enzyme-linked immunosorbent assay(ELISA)and enzyme methods were used to detect MMP-2 and SAA levels,respectively.Results Serum lncRNA ANRIL,MMP-2 and SAA levels in patients with esophageal cancer were significantly higher than those in healthy subjects,and the difference were statistically significant(P<0.05).In addition,the expressions of lncRNA ANRIL,MMP-2 and SAA in serum of patients with esophageal cancer were significantly correlated with TNM stage,histology classification and differentiation degree of tumor(P<0.05),and the levels of lncRNA ANRIL,MMP-2 and SAA in advanced esophageal cancer,esophageal adenosquamous carcinoma and poorly differentiated esophageal cancer patients were increased most significantly.When combined with lncRNA ANRIL,MMP-2 and SAA,the area under the curve(AUC)to distinguish between esophageal cancer and healthy subjects was 0.927(95% CI:0.900～0.954,P<0.05),the sensitivity was 80.1%,specificity was 90.4%.Multivariate analysis showed that serum lncRNA ANRIL,MMP-2 and SAA levels were independent risk factors for esophageal cancer.Conclusion lncRNA ANRIL,MMP-2 and SAA have diagnostic values for esophageal cancer.In addition,lncRNA ANRIL,MMP-2 and SAA can also serve as reference index for predicting the risk of esophageal cancer.
Objective To explore the diagnostic values of long non-coding RNA(lncRNA)ANRIL,matrix metalloproteinase-2(MMP-2)and serum amyloid protein A(SAA)for esophageal cancer,and the relationship between the three and the clinicopathological features of esophageal cancer.Methods 80 cases of esophageal cancer diagnosed in our hospital from January 2016 to December 2017 were collected,and 100 healthy people were recruited in our hospital during the same period.The levels of lncRNA ANRIL in the peripheral blood of subjects were detected by real-time quantitative PCR(RT-PCR).Enzyme-linked immunosorbent assay(ELISA)and enzyme methods were used to detect MMP-2 and SAA levels,respectively.Results Serum lncRNA ANRIL,MMP-2 and SAA levels in patients with esophageal cancer were significantly higher than those in healthy subjects,and the difference were statistically significant(P<0.05).In addition,the expressions of lncRNA ANRIL,MMP-2 and SAA in serum of patients with esophageal cancer were significantly correlated with TNM stage,histology classification and differentiation degree of tumor(P<0.05),and the levels of lncRNA ANRIL,MMP-2 and SAA in advanced esophageal cancer,esophageal adenosquamous carcinoma and poorly differentiated esophageal cancer patients were increased most significantly.When combined with lncRNA ANRIL,MMP-2 and SAA,the area under the curve(AUC)to distinguish between esophageal cancer and healthy subjects was 0.927(95% CI:0.900～0.954,P<0.05),the sensitivity was 80.1%,specificity was 90.4%.Multivariate analysis showed that serum lncRNA ANRIL,MMP-2 and SAA levels were independent risk factors for esophageal cancer.Conclusion lncRNA ANRIL,MMP-2 and SAA have diagnostic values for esophageal cancer.In addition,lncRNA ANRIL,MMP-2 and SAA can also serve as reference index for predicting the risk of esophageal cancer.
引文
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[2] Naveed M,Kubiliun N.Endoscopic treatment of early-stage esophageal cancer[J].Curr Oncol Rep,2018,20(9):71.
[3] 彭卓,牛泽群,万林,等.长链非编码RNA MALAT1与非小细胞肺癌病人肿瘤复发和预后的相关性研究[J].实用癌症杂志,2017,32(7):1056-1058,1072.
[4] 李鑫恒,李正龙,郑汪洋,等.MALAT-1与消化系统肿瘤关系的研究[J].标记免疫分析与临床,2017,24(4):478-480.
[5] Yang Y,Tang T,Peng W,et al.The comparison of miR-155 with computed tomography and computed tomography plus serum amyloid A protein in staging rectal cancer [J].J Surg Res,2015,193(2):764-771.
[6] Lan WG,Xu DH,Xu C,et al.Silencing of long non-coding RNA ANRIL inhibits the development of multidrug resistance in gastric cancer cells[J].Oncol Rep,2016,36(1):263-270.
[7] Ma J,Li T,Han X,et al.Knockdown of LncRNA ANRIL suppresses cell proliferation,metastasis,and invasion via regulating miR-122-5p expression in hepatocellular carcinoma[J].J Cancer Res Clin Oncol,2018,144(2):205-214.
[8] Chen R,Xia W,Wang X,et al.Upregulated long non-coding RNA SBF2-AS1 promotes proliferation in esophageal squamous cell carcinoma[J].Oncol Lett,2018,15(4):5071-5080.
[9] Huang ZL,Chen RP,Zhou XT,et al.Long non-coding RNA MEG3 induces cell apoptosis in esophageal cancer through endoplasmic reticulum stress[J].Oncol Rep,2017,37(5):3093-3099.
[10] Pei L,He X,Li S,et al.KRAB zinc-finger protein 382 regulates epithelial-mesenchymal transition and functions as a tumor suppressor,but is silenced by CpG methylation in gastric cancer[J].Int J Oncol,2018,53(3):961-972.