崇明岛地方菜用大豆指纹图谱构建及遗传多样性分析
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摘要
利用大豆的13对核心SSR引物,对来自上海市崇明岛的61份菜用大豆品种(系)的基因组DNA进行PCR扩增。结果表明,有7对引物在61份菜用大豆之间具有稳定的多态性。一共有31个等位基因被这7对多态性引物检测到,每对引物检测到的等位基因为3～7个,其平均变异数为4.43个。利用这7对SSR引物对61份菜用大豆品种(系)进行遗传相似性分析,构建了每份品种(系)的遗传图谱。根据非加权类平均法进行聚类分析,61份菜用大豆品种(系)间遗传相似系数为0.57～1.0,以相似系数0.79为划分标准,可将其分为9个类别。并根据其主要植物学特性和籽粒特征,将这61份菜用大豆也分为9大类型,但是基于农艺性状和SSR多态性所分类群间存在较大的差异。本研究在一定程度上反映了崇明岛的菜用大豆品种资源之间的亲缘关系,为充分利用崇明岛菜用大豆地方品种资源、拓宽育种遗传基础提供了理论依据。
The genomic DNAs of 61 local vegetable soybean cultivars(lines) from Shanghai Chongming Island were amplified by 13 pairs of core SSR primers of soybean. The results showed that 7 out of 13 pairs of SSR primers could generate stable polymorphism among the 61 vegetable soybean materials. 31 alleles were detected by the 7 pairs of SSR primers, and the number of alleles per locus was ranged from 3 to 7 with an average number4.43. The genetic similarity of the 61 vegetable soybean varieties(lines) was analyzed by using 7 pairs of SSR primers, and the fingerprint of each cultivar(line) was established. The cluster analysis by a non-weighted mean method indicated that the genetic similarity coefficients of the 61 vegetable soybean cultivars(lines) ranged from0.57 to 1.00, and at the 0.79 level, all the 61 vegetable soybean materials could be divided into 9 groups.Moreover, the 61 soybean vegetable cultivars(lines) were classified into 9 groups according to the main botany performance and dried-seed characteristics, but the 9 groups were different with the groups divided by SSR polymorphism. The study revealed the genetic relationship among vegetable soybean cultivars(lines) from Chongming Island to some extent, which could provide theoretical basis for the full use of local vegetable soybean resources from Chongming Island, as well as broaden the genetic diversity of soybean breeding.
The genomic DNAs of 61 local vegetable soybean cultivars(lines) from Shanghai Chongming Island were amplified by 13 pairs of core SSR primers of soybean. The results showed that 7 out of 13 pairs of SSR primers could generate stable polymorphism among the 61 vegetable soybean materials. 31 alleles were detected by the 7 pairs of SSR primers, and the number of alleles per locus was ranged from 3 to 7 with an average number4.43. The genetic similarity of the 61 vegetable soybean varieties(lines) was analyzed by using 7 pairs of SSR primers, and the fingerprint of each cultivar(line) was established. The cluster analysis by a non-weighted mean method indicated that the genetic similarity coefficients of the 61 vegetable soybean cultivars(lines) ranged from0.57 to 1.00, and at the 0.79 level, all the 61 vegetable soybean materials could be divided into 9 groups.Moreover, the 61 soybean vegetable cultivars(lines) were classified into 9 groups according to the main botany performance and dried-seed characteristics, but the 9 groups were different with the groups divided by SSR polymorphism. The study revealed the genetic relationship among vegetable soybean cultivars(lines) from Chongming Island to some extent, which could provide theoretical basis for the full use of local vegetable soybean resources from Chongming Island, as well as broaden the genetic diversity of soybean breeding.
引文
Gai J.Y.,Xu D.H.,Gao Z.,Shimamoto Y.,Abe J.,Fukushi H.,and Kitajima S.,2000,Studies on the evolutionary relationship among eco-types of G.max and G.soja in China,Zuowu Xuebao(Acta Agronomica Sinica),26(5):513-520(盖钧镒,许东河,高忠,岛本义也,阿部纯,福士泰史,北岛俊二,2000,中国栽培大豆和野生大豆不同生态类型群体间遗传演化关系的研究,作物学报,26(5):513-520)
Gai J.Y.,Zhao T.J.,Cui Z.L.,and Qiu J.X.,1998,Nuclear and cytoplasmic contributions of germplasm from distinct areas to the soybean cultivars released during 1923-1995 in China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),31(5):35-43(盖钧镒,赵团结,崔章林,邱家驯,1998,中国大豆育成品种中不同地理来源种质的遗传贡献,中国农业科学,31(5):35-43)
Gao Y.L.,Yao B.C.,Liu C.Y.,Li W.F.,Jiang H.W.,Li C.D.,Zhang W.B.,Hu G.H.,and Chen Q.S.,2009,Genetic diversity analysis by simple sequence repeats of soybean(Glycine max)varieties from Heilongjiang,Zhiwu Xuebao(Chinese Bulletin of Botany),44(5):556-561(高运来,姚丙晨,刘春燕,李文福,蒋洪蔚,李灿东,张闻博,胡国华,陈庆山,2009,黑龙江省主栽大豆品种遗传多样性的SSR分析,植物学报,44(5):556-561)
Guan R.X.,Fang H.L.,He Y.Q.,Chang R.Z.,and Qiu L.J.,2012,Molecular homozygosity of soybean varieties(lines)in regional test of China by using SSR markers,Zuowu Xuebao(Acta Agronomica Sinica),38(10):1760-1765(关荣霞,方宏亮,何艳琴,常汝镇,邱丽娟,2012,国家大豆区域试验品种(系)SSR位点纯合度分析,作物学报,38(10):1760-1765)
Han X.Q.,Lin Y.H.,Zhang L.F.,Hao Y.,and Zhang L.J.,2008,Analysis of genetic diversity by SSR for Shandong soybean cultivars in different years,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),24(1):74-77(韩雪芹,林延慧,张礼凤,蒿燕,张丽娟,2008,山东省不同年代栽培大豆SSR标记遗传多样性分析,中国农学通报,24(1):74-77)
Li Y.H.,Chang R.Z.,and Qiu L.J.,2010,The strategy for maintaining genetic integrity of soybean germplasms:selecting pure lines based on SSR markers,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(19):3930-3936(李英慧,常汝镇,邱丽娟,2010,保存大豆种质遗传完整性的策略--基于SSR分子标记选择纯系,中国农业科学,43(19):3930-3936)
Lou Q.J.,Chen L.,and Luo L.J.,2005,Comparison of three rapid methods of DNA extraction from rice,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),3(5):749-752(楼巧君,陈亮,罗利军,2005,三种水稻基因DNA快速提取法的比较,分子植物育种,3(5):749-752)
Song R.H.,Zhu L.H.,Gu W.H.,Yang H.J.,Ma K.,Zhi H.J.,and Shan Z.H.,2014,Evaluation of agronomic characteristics for soybean landraces from Shanghai Chongming Islands,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(6):1182-1187(宋荣浩,朱丽华,顾卫红,杨红娟,马坤,智海剑,单志慧,2014,上海地方大豆品种资源主要农艺性状的鉴定评价,植物遗传资源学报,15(6):1182-1187)
Gai J.Y.,Zhao T.J.,Cui Z.L.,and Qiu J.X.,1998,Nuclear and cytoplasmic contributions of germplasm from distinct areas to the soybean cultivars released during 1923-1995 in China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),31(5):35-43(盖钧镒,赵团结,崔章林,邱家驯,1998,中国大豆育成品种中不同地理来源种质的遗传贡献,中国农业科学,31(5):35-43)
Gao Y.L.,Yao B.C.,Liu C.Y.,Li W.F.,Jiang H.W.,Li C.D.,Zhang W.B.,Hu G.H.,and Chen Q.S.,2009,Genetic diversity analysis by simple sequence repeats of soybean(Glycine max)varieties from Heilongjiang,Zhiwu Xuebao(Chinese Bulletin of Botany),44(5):556-561(高运来,姚丙晨,刘春燕,李文福,蒋洪蔚,李灿东,张闻博,胡国华,陈庆山,2009,黑龙江省主栽大豆品种遗传多样性的SSR分析,植物学报,44(5):556-561)
Guan R.X.,Fang H.L.,He Y.Q.,Chang R.Z.,and Qiu L.J.,2012,Molecular homozygosity of soybean varieties(lines)in regional test of China by using SSR markers,Zuowu Xuebao(Acta Agronomica Sinica),38(10):1760-1765(关荣霞,方宏亮,何艳琴,常汝镇,邱丽娟,2012,国家大豆区域试验品种(系)SSR位点纯合度分析,作物学报,38(10):1760-1765)
Han X.Q.,Lin Y.H.,Zhang L.F.,Hao Y.,and Zhang L.J.,2008,Analysis of genetic diversity by SSR for Shandong soybean cultivars in different years,Zhongguo Nongxue Tongbao(Chinese Agricultural Science Bulletin),24(1):74-77(韩雪芹,林延慧,张礼凤,蒿燕,张丽娟,2008,山东省不同年代栽培大豆SSR标记遗传多样性分析,中国农学通报,24(1):74-77)
Li Y.H.,Chang R.Z.,and Qiu L.J.,2010,The strategy for maintaining genetic integrity of soybean germplasms:selecting pure lines based on SSR markers,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(19):3930-3936(李英慧,常汝镇,邱丽娟,2010,保存大豆种质遗传完整性的策略--基于SSR分子标记选择纯系,中国农业科学,43(19):3930-3936)
Lou Q.J.,Chen L.,and Luo L.J.,2005,Comparison of three rapid methods of DNA extraction from rice,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),3(5):749-752(楼巧君,陈亮,罗利军,2005,三种水稻基因DNA快速提取法的比较,分子植物育种,3(5):749-752)
Song R.H.,Zhu L.H.,Gu W.H.,Yang H.J.,Ma K.,Zhi H.J.,and Shan Z.H.,2014,Evaluation of agronomic characteristics for soybean landraces from Shanghai Chongming Islands,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),15(6):1182-1187(宋荣浩,朱丽华,顾卫红,杨红娟,马坤,智海剑,单志慧,2014,上海地方大豆品种资源主要农艺性状的鉴定评价,植物遗传资源学报,15(6):1182-1187)