摘要
利用大豆的13对核心SSR引物,对来自上海市崇明岛的61份菜用大豆品种(系)的基因组DNA进行PCR扩增。结果表明,有7对引物在61份菜用大豆之间具有稳定的多态性。一共有31个等位基因被这7对多态性引物检测到,每对引物检测到的等位基因为3~7个,其平均变异数为4.43个。利用这7对SSR引物对61份菜用大豆品种(系)进行遗传相似性分析,构建了每份品种(系)的遗传图谱。根据非加权类平均法进行聚类分析,61份菜用大豆品种(系)间遗传相似系数为0.57~1.0,以相似系数0.79为划分标准,可将其分为9个类别。并根据其主要植物学特性和籽粒特征,将这61份菜用大豆也分为9大类型,但是基于农艺性状和SSR多态性所分类群间存在较大的差异。本研究在一定程度上反映了崇明岛的菜用大豆品种资源之间的亲缘关系,为充分利用崇明岛菜用大豆地方品种资源、拓宽育种遗传基础提供了理论依据。
The genomic DNAs of 61 local vegetable soybean cultivars(lines) from Shanghai Chongming Island were amplified by 13 pairs of core SSR primers of soybean. The results showed that 7 out of 13 pairs of SSR primers could generate stable polymorphism among the 61 vegetable soybean materials. 31 alleles were detected by the 7 pairs of SSR primers, and the number of alleles per locus was ranged from 3 to 7 with an average number4.43. The genetic similarity of the 61 vegetable soybean varieties(lines) was analyzed by using 7 pairs of SSR primers, and the fingerprint of each cultivar(line) was established. The cluster analysis by a non-weighted mean method indicated that the genetic similarity coefficients of the 61 vegetable soybean cultivars(lines) ranged from0.57 to 1.00, and at the 0.79 level, all the 61 vegetable soybean materials could be divided into 9 groups.Moreover, the 61 soybean vegetable cultivars(lines) were classified into 9 groups according to the main botany performance and dried-seed characteristics, but the 9 groups were different with the groups divided by SSR polymorphism. The study revealed the genetic relationship among vegetable soybean cultivars(lines) from Chongming Island to some extent, which could provide theoretical basis for the full use of local vegetable soybean resources from Chongming Island, as well as broaden the genetic diversity of soybean breeding.
引文
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