利用InDel和SSILP标记分析北方粳型超级稻的遗传组成
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摘要
为明确籼稻血缘对北方粳型超级稻育种的贡献,利用89对籼粳特异性引物(34对InDel和55对SSILP)对15份粳型超级稻品种以及与其系谱密切相关的21份材料(12份1977-1999年育成的常规粳稻品种和9份1963-2000年育成的日本品种)的遗传组成进行分析。结果表明,15份粳型超级稻品种均含有一定的籼型血缘,且不同地区超级稻品种所含籼型基因频率(Fi)有所不同;对不同年代育成的参试材料的Fi进行比较分析,发现超级稻品种籼型基因型频率最高,Fi为0.068;12份常规粳稻品种次之,Fi为0.033;9份日本品种几乎不含有籼型血缘,Fi为0.011;表明随着时间的推进,近现代以来育成品种的籼型基因频率逐渐增加。
Genetic background of 15super-rice and their 21pedigree-relative varieties(including 12cultivars released from 1977to 1999in northeast and 9japanese varieties bred from 1963to 2000)were revealed based on 89subspecificdifferentiation molecular markers(34InDel and 55SSILP).The results showed that indica linage have already introgressed into the genomes of 15super-rice varieties at different level which represented by indica-type frequency(Fi).The Fi variation of super-rice varieties reflected registration periods difference,varieties bred in 2005-2011were the highest(Fi=0.068),followed by 1977-1999(Fi=0.033)and rarely in 1963-2000(Fi=0.011).Consequently,indica-allele frequencies of recent varieties were gradually increased.And this fact demonstrates that a certain proportion of indica linage introducing is an important strategy to the increased yield of northern japonica rice.
Genetic background of 15super-rice and their 21pedigree-relative varieties(including 12cultivars released from 1977to 1999in northeast and 9japanese varieties bred from 1963to 2000)were revealed based on 89subspecificdifferentiation molecular markers(34InDel and 55SSILP).The results showed that indica linage have already introgressed into the genomes of 15super-rice varieties at different level which represented by indica-type frequency(Fi).The Fi variation of super-rice varieties reflected registration periods difference,varieties bred in 2005-2011were the highest(Fi=0.068),followed by 1977-1999(Fi=0.033)and rarely in 1963-2000(Fi=0.011).Consequently,indica-allele frequencies of recent varieties were gradually increased.And this fact demonstrates that a certain proportion of indica linage introducing is an important strategy to the increased yield of northern japonica rice.
引文
[1]陈温福,徐正进.水稻超高产育种理论与方法.北京:科学出版社,2007:1-14.
[2]陈温福,徐正进,张龙步,等.水稻超高产育种研究进展与前景.中国工程科学,2002,4(1):31-35.
[3]陈温福.北方水稻生产技术问答.北京:中国农业出版社,2010:1-2.
[4]李红宇,张龙海,刘梦红,等.东北地区水稻品种与日本引进品种遗传多样性比较.核农学报,2011,25(6):1082-1087.
[5]李红宇,侯昱铭,陈英华,等.用SSR标记评估东北三省水稻推广品种的遗传多样性.中国水稻科学,2009,23(4):383-390.
[6]杨守仁.籼粳稻杂交育种研究.遗传学通讯,1973(2):34-38.
[7]杨守仁,张龙步,沈锡英,等.三十六年来籼粳稻杂交育种的研究及发展.沈阳农业大学学报,1987,18(3):3-9.
[8]张喜娟,李伟娟,李红娇,等.超级稻沈农265生长发育特性及产量形成特点.华中农业大学学报,2008,27(4):445-450.
[9]吴文革,张洪程,吴桂成,等.超级稻群体籽粒库容特征的初步研究.中国农业学,2007,40(2):250-257.
[10]孙国宏,徐海,李景波,等.北方粳型超级稻群体生长发育及冠层特征研究.作物杂志,2010(4):420-423.
[11]许明,贾德涛,马殿荣,等.北方粳型超级稻灌浆结实期碳水化合物含量和保护酶活性的变化.中国水稻科学,2011,25(1):59-64.
[12]贾德涛,许明,王楠,等.北方粳型超级稻沈农265根系特征的初步研究.华中农业大学学报,2007,26(4):443-447.
[13]卢宝荣,蔡星星,金鑫.籼稻和粳稻的高效分子鉴定方法及其在水稻育种和进化研究中的意义.自然科学进展,2009,19(6):628-639.
[14]赵向前,吴为人.水稻ILP标记遗传图谱的构建遗传.遗传,2008,30(2):225-230.
[15]Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochem Bull,1987,19:11-15.
[16]James R F.Numerical taxonomy and multivariate analysis system v2.1user guide.Applied biostatistics Inc.,2000:29-38.
[17]万建民.中国水稻遗传育种与品种系谱.北京:中国农业出版社,2010:208-369.
[18]国家水稻数据中心.http://www.ricedata.cn/variety/,2013-01.
[19]林世成,闵绍楷.中国水稻品种及其系谱.上海:科学技术出版社,1991:254-262.
[20]刘迪,李红宇,孙健,等.应用SSR分子标记评价不同年代东北三省粳稻基因组遗传构成.黑龙江农业科学,2011(7):1-6.
[21]Sun J,Liu D,Wang J Y,et al.The contribution of intersubspecic hybridization to the breeding of super-highyielding japonica rice in northeast China.Theor Appl Genet,2012,125(6):1149-1157.
[22]高虹,林晗,孙健,等.北方粳型超级稻中籼粳杂交优势贡献的分子基础.沈阳农业大学学报,2012,43(4):394-400.
[23]Li Y B,Fan C C,Xing Y Z,et al.Natural variation in GS5plays an important role in regulating grain size and yield in rice.Nat Genet,2011,43(12):1266-1269.
[24]Weng J F,Gu S H,Wan X Y,et al.Isolation and initial characterization of GW5,a major QTL associated with rice grain width and weight.Cell Res,2008,18(12):1199-1209.
[25]Shomura A,Izawa T,Ebana K,et al.Deletion in a gene associated with grain size increased yields during rice domestication.Nat Genet,2008,40(8):1023-1028.
[26]Ishimaru K,Hirotsu N,Madoka Y,et al.Loss of function of the IAA-glucose hydrolase gene TGW6enhances rice grain weight and increases yield.Nat Genet,2013,45(6):707-711.
[2]陈温福,徐正进,张龙步,等.水稻超高产育种研究进展与前景.中国工程科学,2002,4(1):31-35.
[3]陈温福.北方水稻生产技术问答.北京:中国农业出版社,2010:1-2.
[4]李红宇,张龙海,刘梦红,等.东北地区水稻品种与日本引进品种遗传多样性比较.核农学报,2011,25(6):1082-1087.
[5]李红宇,侯昱铭,陈英华,等.用SSR标记评估东北三省水稻推广品种的遗传多样性.中国水稻科学,2009,23(4):383-390.
[6]杨守仁.籼粳稻杂交育种研究.遗传学通讯,1973(2):34-38.
[7]杨守仁,张龙步,沈锡英,等.三十六年来籼粳稻杂交育种的研究及发展.沈阳农业大学学报,1987,18(3):3-9.
[8]张喜娟,李伟娟,李红娇,等.超级稻沈农265生长发育特性及产量形成特点.华中农业大学学报,2008,27(4):445-450.
[9]吴文革,张洪程,吴桂成,等.超级稻群体籽粒库容特征的初步研究.中国农业学,2007,40(2):250-257.
[10]孙国宏,徐海,李景波,等.北方粳型超级稻群体生长发育及冠层特征研究.作物杂志,2010(4):420-423.
[11]许明,贾德涛,马殿荣,等.北方粳型超级稻灌浆结实期碳水化合物含量和保护酶活性的变化.中国水稻科学,2011,25(1):59-64.
[12]贾德涛,许明,王楠,等.北方粳型超级稻沈农265根系特征的初步研究.华中农业大学学报,2007,26(4):443-447.
[13]卢宝荣,蔡星星,金鑫.籼稻和粳稻的高效分子鉴定方法及其在水稻育种和进化研究中的意义.自然科学进展,2009,19(6):628-639.
[14]赵向前,吴为人.水稻ILP标记遗传图谱的构建遗传.遗传,2008,30(2):225-230.
[15]Doyle J J,Doyle J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue.Phytochem Bull,1987,19:11-15.
[16]James R F.Numerical taxonomy and multivariate analysis system v2.1user guide.Applied biostatistics Inc.,2000:29-38.
[17]万建民.中国水稻遗传育种与品种系谱.北京:中国农业出版社,2010:208-369.
[18]国家水稻数据中心.http://www.ricedata.cn/variety/,2013-01.
[19]林世成,闵绍楷.中国水稻品种及其系谱.上海:科学技术出版社,1991:254-262.
[20]刘迪,李红宇,孙健,等.应用SSR分子标记评价不同年代东北三省粳稻基因组遗传构成.黑龙江农业科学,2011(7):1-6.
[21]Sun J,Liu D,Wang J Y,et al.The contribution of intersubspecic hybridization to the breeding of super-highyielding japonica rice in northeast China.Theor Appl Genet,2012,125(6):1149-1157.
[22]高虹,林晗,孙健,等.北方粳型超级稻中籼粳杂交优势贡献的分子基础.沈阳农业大学学报,2012,43(4):394-400.
[23]Li Y B,Fan C C,Xing Y Z,et al.Natural variation in GS5plays an important role in regulating grain size and yield in rice.Nat Genet,2011,43(12):1266-1269.
[24]Weng J F,Gu S H,Wan X Y,et al.Isolation and initial characterization of GW5,a major QTL associated with rice grain width and weight.Cell Res,2008,18(12):1199-1209.
[25]Shomura A,Izawa T,Ebana K,et al.Deletion in a gene associated with grain size increased yields during rice domestication.Nat Genet,2008,40(8):1023-1028.
[26]Ishimaru K,Hirotsu N,Madoka Y,et al.Loss of function of the IAA-glucose hydrolase gene TGW6enhances rice grain weight and increases yield.Nat Genet,2013,45(6):707-711.