植物中病毒诱导基因沉默技术的研究与应用进展
|
摘要
为了给植物研究中病毒诱导的基因沉默(VIGS)技术的应用提供相关理论参考。本综述通过查阅近几年来相关的国内外文献,从VIGS的技术原理、调控机制、影响因素、应用研究等方面进行归纳和总结。VIGS是通过检测细胞质中病毒的RNA序列,并且对它进行降解的过程,该技术属于RNA介导的,基因转录后沉默的植物抗病毒机制,如今VIGS已经成功地应用于各种模式植物和农作物中的基因功能的正反向研究。现在随着高通量测序技术的不断发展,VIGS技术必将成为大批量基因快速功能鉴定的重要工具之一。
In order to provides references for the application of virus induced gene silencing(VIGS) technology in plant research, this review summarized the technical principle, regulation mechanism, influencing factors and application research of VIGS based on the relevant papers in recent years. VIGS is the process of detecting the RNA sequence of the virus in the cytoplasm and degrading it. It is an RNA mediated post-transcriptional gene silencing mechanism for plant antivirus. So far it has been successfully applied to study the gene function in various model plants and crops. With the continuous development of high-throughput sequencing technology,VIGS technology will be one of the important tools for the rapid functional identification of large number of genes.
In order to provides references for the application of virus induced gene silencing(VIGS) technology in plant research, this review summarized the technical principle, regulation mechanism, influencing factors and application research of VIGS based on the relevant papers in recent years. VIGS is the process of detecting the RNA sequence of the virus in the cytoplasm and degrading it. It is an RNA mediated post-transcriptional gene silencing mechanism for plant antivirus. So far it has been successfully applied to study the gene function in various model plants and crops. With the continuous development of high-throughput sequencing technology,VIGS technology will be one of the important tools for the rapid functional identification of large number of genes.
引文
Atwood S.E.,O'Rourke J.A.,Peiffer G.A.,Yin T.F.,Majumder M.,Zhang C.Q.,Cianzio S.R.,Hill J.H.,Cook D.,Whitham S.A.,Shoemaker R.C.,and Graham M.A.,2014,Replication protein A subunit 3 and the iron efficiency response in soybean,Plant Cell Environ.,37:213-234
Bruun-Rasmussen M.,Madsen C.T.,Jessing S.,and Albrechtsen M.,2007,Stability of Barley stripe mosaic virus-induced gene silencing in barley,Mol.Plant Microbe Interact.,20(11):1323-1331
Burch-Smith T.M.,Anderson J.C.,Martin G.B.,and Dinesh-Kumar S.P.,2010,Applications and advantages of virus-induced gene silencing for gene function studies in plants,Plant J.,39(5):734-746
Cai Q.F.,Tang Q.J.,Shen W.T.,and Zhou P.,2005,Applications of plant virus induced gene silencing for gene function studies,Shengming Kexue Yanjiu(Life Science Research),9(4):50-55(蔡群芳,唐清杰,沈文涛,周鹏,2005,植物病毒诱导的基因沉默在基因功能研究中的应用,生命科学研究,9(4):50-55)
Deng X.,Elomaa P.,Nguyen C.X.,Hytonen T.,Valkonen J.P.,and Teeri T.H.,2012,Virus-induced gene silencing for Asteraceae-a reverse genetics approach for functional genomics in Gerbera hybrid,Plant Biotechnol.J.,10(8):970-978
Di-Stilio V.S.,Kumar R.A.,Oddone A.M.,Tolkin T.R.,Salles P.,and Mccarty K.,2010,Virus-induced gene silencing as a tool for comparative functional studies in Thalictrum,PLoSOne,5(8):e12064
Ekengren S.K.,Liu Y.,Schiff M.,Dinesh-Kumar S.P.,and Martin G.B.,2003,Two MAPK cascades,NPR1,and TGA transcription factors play a role in Pto-mediated disease resistance in tomato,Plant J.,36:905-917
Fridborg I.,Johansson A.,Lagensjo J.,Leelarasamee N.,Flokova K.,Tarkowska D.,Meijer J.,and Bejai S.,2013,ML3:a novel regulator of herbivory-induced responses in Arabidopsis thaliana,J.Exp.Bot.,64(4):935-948
Guo J.,Bai P.,Yang Q.,Liu F.,Wang X.,Huang L.,and Kang Z.,2013,Wheat zinc finger protein TaLSD1,a negative regulator of programmed cell death,is involved in wheat resistance against stripe rust fungus,Plant Physiol.Biochem.,71(2):164-172
Kang G.Z.,Li G.Z.,Ma H.Z.,Wang C.Y.,and Guo T.C.,2013,Proteomic analysis on the leaves of TaBTF3 gene virus-induced silenced wheat plants may reveal its regulatory mechanism,J.Proteomics,83(10):130-143
Kawalek A.,Dmochowska-Boguta M.,Nadolska-Orczyk A.,and Orczyk W.,2012,A new BSMV-based vector with modifiedβmolecule allows simultaneous and stable silencing of two genes,Cell.Mol.Biol.Lett.,17(1):107-123
Lim S.,Nam M.,Kim K.H.,Lee S.H.,Moon J.K.,Lim H.S.,Choung M.G.,Kim S.M.,and Moon J.S.,2016,Development of a new vector using Soybean yellow common mosaic virus for gene function study or heterologous protein expression in soybeans,J.Virol.Methods,228(4):1-9
Liu E.,and Page J.,2008,Optimized cDNA libraries for virus-induced gene silencing(VIGS)using tobacco rattle virus,Plant Methods,4:5
Liu H.,2012,Study on tissue culture of Camellia oleifera and application of virus-induced gene silencing in C.oleifera and Vernicia fordii,Thesis for M.S.,Southwest University,Supervisor:Luo K.M.,pp.29-33(刘虹,2012,油茶组培体系的建立及VIGS系统在油茶、油桐基因功能研究中的应用,硕士学位论文,西南大学,导师:罗克明,pp.29-33)
Lu L.L.,Fu D.Q.,Liu H.P.,Wang X.H.,Luo Y.B.,Zhu B.Z.,and Zuo J.H.,2011,Construction and identification VIGS vector about vitamin E biosynthesis gene of tomato,Beifang Yuanyi(Northern Horticulture),(16):139-142(芦亮亮,傅达奇,刘海萍,王晓辉,罗云波,朱本忠,左进华,2011,番茄中维生素E合成相关基因的VIGS载体构建及侵染,北方园艺,(16):139-142)
Niu Y.L.,Jiang X.M.,and Xu X.Y.,2016,Cloning and VIGSvector construction of GRAS transcription factors family gene SIGLD1 from tomato,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(8):2155-2160(牛义岭,姜秀明,许向阳,2016,番茄GRAS转录因子家族基因SIGLD1的克隆及VIGS载体构建,基因组学与应用生物学,35(8):2155-2160)
Palrner K.E.,Thomson J.A.,and Rybicki E.P.,1999,Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors,Arch.Virol.,144(7):1345-1360
Peele C.,Jordan C.V.,Muangsan N.,Turnage M.,Egelkrout E.,Eagle P.,Hanley-Bowdoin L.,and Robertson D.,2001,Silencing of a meristematic gene using geminivirus-derived vectors,Plant J.,27(4):357-366
Ramegowda V.,Mysore K.S.,and Senthil-Kumar M.,2014,Virusinduced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants,Front.Plant Sci.,5:323
Senthil-Kumar M.,and Mysore K.S.,2011,New dimensions for VIGS in plant functional genomics,Trends Plant Sci.,16(12):656-665
Spitzer B.,Zvi M.M.B.,Ovadis M.,Marhevka E.,Barkai O.,Edelbaum O.,Marton I.,Masci T.,Alon M.,Morin S.,Rogachev I.,Aharoni S.,and Vainstein A.,2007,Reverse genetics of floral scent:application of tobacco rattle virus-based gene silencing in Petunia,Plant Physiol.,145(4):1241-1250
Tang L.,2012,Cloning and functional analysis of Tanacetum cinerariifolium CDS_CCI2 gene and establishment of genetic transformation system in T.cinerariifolium,Dissertation for Ph.D.,Chongqing University,Supervisor:Li Z.G.,pp.55-59(汤玲,2012,除虫菊CDS_CCI2基因的克隆、功能分析及遗传转化体系建立,博士学位论文,重庆大学,导师:李正国,pp.55-59)
Tao X.R.,and Zhou X.P.,2004,A modified viral satellite DNAthat suppresses gene expression in plants,Plant J.,38(5):850-860
Tuttle J.R.,Haigler C.H.,and Robertson D.,2012,Method:low-cost delivery of the cotton leaf crumple virus-induced gene silencing system,Plant Methods,8(1):27
Wang M.,2012,The molecular evolution of NRG1 and its role in resistance against TMV in different Nicotiana species,Thesis for M.S.,Huazhong Agricultural University,Supervisor:Kuang H.H.,pp.17-22(王敏,2012,NRG1的分子进化及其在不同烟草物种TMV抗性中的作用,硕士学位论文,华中农业大学,导师:匡汉晖,pp.17-22)
Wang Y.R.,and Yao Y.C.,2015,Application of TRV-GFP vector in strawflower plants,Beijing Nongxueyuan Xuebao(Journal of Beijing University of Agriculture),30(4):33-37(王亚茹,姚允冲,2015,TRV-GFP载体在麦秆菊上的应用,北京农学院学报,30(4):33-37)
Wijekoon C.P.,and Facchini P.J.,2012,Systematic knockdown of morphine pathway enzymes in opium poppy using virusinduced gene silencing,Plant J.,69(6):1052-1063
Xu Y.P.,Xu Q.F.,Song X.Y.,Zhang Z.X.,and Cai X.Z.,2008,Virus-induced gene silencing,Zhejiang Daxue Xuebao(Journal of Zhejiang University(Agriculture and Life Sciences)),34(2):119-131(徐幼平,徐秋芳,宋晓毅,张志新,蔡新忠,2008,病毒诱导的基因沉默,浙江大学学报(农业与生命科学版),34(2):119-131)
Yao D.Q.,Zhang W.W.,Yuan L.H.,Pan J.S.,He H.L.,and Cai R.,2009,VIGS:the revolution of plant function genomics research,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),7(1):155-161(姚丹青,张微微,原丽华,潘俊松,何欢乐,蔡润,2009,VIGS:植物功能基因组学研究的革命,分子植物育种,7(1):155-161)
Zhang X.L.,Zhang Z.W.,Na R.,Yang S.Q.,Gao J.,and Zhao J.,2014,The effects of different potato genotypes and different inoculation methods on VIGS silence efficiency,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),29(1):36-40(张晓萝,张之为,娜仁,杨叔清,高婧,赵君,2014,马铃薯中VIGS沉默体系的研究,华北农学报,29(1):36-40)
Zhang Z.Y.,Yao W.L.,and Xin Z.Y.,2006,Advances on virusinduced gene silencing for gene function characteriza tion in plants,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),7(1):100-105(张增艳,姚乌兰,辛志勇,2006,植物基因功能鉴定新工具-病毒诱导基因沉默技术的研究进展,植物遗传资源学报,7(1):100-105)
Zheng S.J.,Snoeren T.A.,Hogewoning S.W.,VanLoon J.J.,and Dicke M.,2010,Disruption of plant carotenoid biosynthesis through virus-induced gene silencing affects oviposition behaviour of the butterfly Pieris rapae,New Phytol.,186(3):733-745
Zhou X.F.,Sun J.D.,Zhao Z.,Lv J.,Wei X.W.,Cai R.,and Xu H.W.,2010,The feasibility analysis of PVX and TRV vectors as the VIGS tool for studying the gene function,Physics Procedia,33:46-54
Bruun-Rasmussen M.,Madsen C.T.,Jessing S.,and Albrechtsen M.,2007,Stability of Barley stripe mosaic virus-induced gene silencing in barley,Mol.Plant Microbe Interact.,20(11):1323-1331
Burch-Smith T.M.,Anderson J.C.,Martin G.B.,and Dinesh-Kumar S.P.,2010,Applications and advantages of virus-induced gene silencing for gene function studies in plants,Plant J.,39(5):734-746
Cai Q.F.,Tang Q.J.,Shen W.T.,and Zhou P.,2005,Applications of plant virus induced gene silencing for gene function studies,Shengming Kexue Yanjiu(Life Science Research),9(4):50-55(蔡群芳,唐清杰,沈文涛,周鹏,2005,植物病毒诱导的基因沉默在基因功能研究中的应用,生命科学研究,9(4):50-55)
Deng X.,Elomaa P.,Nguyen C.X.,Hytonen T.,Valkonen J.P.,and Teeri T.H.,2012,Virus-induced gene silencing for Asteraceae-a reverse genetics approach for functional genomics in Gerbera hybrid,Plant Biotechnol.J.,10(8):970-978
Di-Stilio V.S.,Kumar R.A.,Oddone A.M.,Tolkin T.R.,Salles P.,and Mccarty K.,2010,Virus-induced gene silencing as a tool for comparative functional studies in Thalictrum,PLoSOne,5(8):e12064
Ekengren S.K.,Liu Y.,Schiff M.,Dinesh-Kumar S.P.,and Martin G.B.,2003,Two MAPK cascades,NPR1,and TGA transcription factors play a role in Pto-mediated disease resistance in tomato,Plant J.,36:905-917
Fridborg I.,Johansson A.,Lagensjo J.,Leelarasamee N.,Flokova K.,Tarkowska D.,Meijer J.,and Bejai S.,2013,ML3:a novel regulator of herbivory-induced responses in Arabidopsis thaliana,J.Exp.Bot.,64(4):935-948
Guo J.,Bai P.,Yang Q.,Liu F.,Wang X.,Huang L.,and Kang Z.,2013,Wheat zinc finger protein TaLSD1,a negative regulator of programmed cell death,is involved in wheat resistance against stripe rust fungus,Plant Physiol.Biochem.,71(2):164-172
Kang G.Z.,Li G.Z.,Ma H.Z.,Wang C.Y.,and Guo T.C.,2013,Proteomic analysis on the leaves of TaBTF3 gene virus-induced silenced wheat plants may reveal its regulatory mechanism,J.Proteomics,83(10):130-143
Kawalek A.,Dmochowska-Boguta M.,Nadolska-Orczyk A.,and Orczyk W.,2012,A new BSMV-based vector with modifiedβmolecule allows simultaneous and stable silencing of two genes,Cell.Mol.Biol.Lett.,17(1):107-123
Lim S.,Nam M.,Kim K.H.,Lee S.H.,Moon J.K.,Lim H.S.,Choung M.G.,Kim S.M.,and Moon J.S.,2016,Development of a new vector using Soybean yellow common mosaic virus for gene function study or heterologous protein expression in soybeans,J.Virol.Methods,228(4):1-9
Liu E.,and Page J.,2008,Optimized cDNA libraries for virus-induced gene silencing(VIGS)using tobacco rattle virus,Plant Methods,4:5
Liu H.,2012,Study on tissue culture of Camellia oleifera and application of virus-induced gene silencing in C.oleifera and Vernicia fordii,Thesis for M.S.,Southwest University,Supervisor:Luo K.M.,pp.29-33(刘虹,2012,油茶组培体系的建立及VIGS系统在油茶、油桐基因功能研究中的应用,硕士学位论文,西南大学,导师:罗克明,pp.29-33)
Lu L.L.,Fu D.Q.,Liu H.P.,Wang X.H.,Luo Y.B.,Zhu B.Z.,and Zuo J.H.,2011,Construction and identification VIGS vector about vitamin E biosynthesis gene of tomato,Beifang Yuanyi(Northern Horticulture),(16):139-142(芦亮亮,傅达奇,刘海萍,王晓辉,罗云波,朱本忠,左进华,2011,番茄中维生素E合成相关基因的VIGS载体构建及侵染,北方园艺,(16):139-142)
Niu Y.L.,Jiang X.M.,and Xu X.Y.,2016,Cloning and VIGSvector construction of GRAS transcription factors family gene SIGLD1 from tomato,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(8):2155-2160(牛义岭,姜秀明,许向阳,2016,番茄GRAS转录因子家族基因SIGLD1的克隆及VIGS载体构建,基因组学与应用生物学,35(8):2155-2160)
Palrner K.E.,Thomson J.A.,and Rybicki E.P.,1999,Generation of maize cell lines containing autonomously replicating maize streak virus-based gene vectors,Arch.Virol.,144(7):1345-1360
Peele C.,Jordan C.V.,Muangsan N.,Turnage M.,Egelkrout E.,Eagle P.,Hanley-Bowdoin L.,and Robertson D.,2001,Silencing of a meristematic gene using geminivirus-derived vectors,Plant J.,27(4):357-366
Ramegowda V.,Mysore K.S.,and Senthil-Kumar M.,2014,Virusinduced gene silencing is a versatile tool for unraveling the functional relevance of multiple abiotic-stress-responsive genes in crop plants,Front.Plant Sci.,5:323
Senthil-Kumar M.,and Mysore K.S.,2011,New dimensions for VIGS in plant functional genomics,Trends Plant Sci.,16(12):656-665
Spitzer B.,Zvi M.M.B.,Ovadis M.,Marhevka E.,Barkai O.,Edelbaum O.,Marton I.,Masci T.,Alon M.,Morin S.,Rogachev I.,Aharoni S.,and Vainstein A.,2007,Reverse genetics of floral scent:application of tobacco rattle virus-based gene silencing in Petunia,Plant Physiol.,145(4):1241-1250
Tang L.,2012,Cloning and functional analysis of Tanacetum cinerariifolium CDS_CCI2 gene and establishment of genetic transformation system in T.cinerariifolium,Dissertation for Ph.D.,Chongqing University,Supervisor:Li Z.G.,pp.55-59(汤玲,2012,除虫菊CDS_CCI2基因的克隆、功能分析及遗传转化体系建立,博士学位论文,重庆大学,导师:李正国,pp.55-59)
Tao X.R.,and Zhou X.P.,2004,A modified viral satellite DNAthat suppresses gene expression in plants,Plant J.,38(5):850-860
Tuttle J.R.,Haigler C.H.,and Robertson D.,2012,Method:low-cost delivery of the cotton leaf crumple virus-induced gene silencing system,Plant Methods,8(1):27
Wang M.,2012,The molecular evolution of NRG1 and its role in resistance against TMV in different Nicotiana species,Thesis for M.S.,Huazhong Agricultural University,Supervisor:Kuang H.H.,pp.17-22(王敏,2012,NRG1的分子进化及其在不同烟草物种TMV抗性中的作用,硕士学位论文,华中农业大学,导师:匡汉晖,pp.17-22)
Wang Y.R.,and Yao Y.C.,2015,Application of TRV-GFP vector in strawflower plants,Beijing Nongxueyuan Xuebao(Journal of Beijing University of Agriculture),30(4):33-37(王亚茹,姚允冲,2015,TRV-GFP载体在麦秆菊上的应用,北京农学院学报,30(4):33-37)
Wijekoon C.P.,and Facchini P.J.,2012,Systematic knockdown of morphine pathway enzymes in opium poppy using virusinduced gene silencing,Plant J.,69(6):1052-1063
Xu Y.P.,Xu Q.F.,Song X.Y.,Zhang Z.X.,and Cai X.Z.,2008,Virus-induced gene silencing,Zhejiang Daxue Xuebao(Journal of Zhejiang University(Agriculture and Life Sciences)),34(2):119-131(徐幼平,徐秋芳,宋晓毅,张志新,蔡新忠,2008,病毒诱导的基因沉默,浙江大学学报(农业与生命科学版),34(2):119-131)
Yao D.Q.,Zhang W.W.,Yuan L.H.,Pan J.S.,He H.L.,and Cai R.,2009,VIGS:the revolution of plant function genomics research,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),7(1):155-161(姚丹青,张微微,原丽华,潘俊松,何欢乐,蔡润,2009,VIGS:植物功能基因组学研究的革命,分子植物育种,7(1):155-161)
Zhang X.L.,Zhang Z.W.,Na R.,Yang S.Q.,Gao J.,and Zhao J.,2014,The effects of different potato genotypes and different inoculation methods on VIGS silence efficiency,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),29(1):36-40(张晓萝,张之为,娜仁,杨叔清,高婧,赵君,2014,马铃薯中VIGS沉默体系的研究,华北农学报,29(1):36-40)
Zhang Z.Y.,Yao W.L.,and Xin Z.Y.,2006,Advances on virusinduced gene silencing for gene function characteriza tion in plants,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),7(1):100-105(张增艳,姚乌兰,辛志勇,2006,植物基因功能鉴定新工具-病毒诱导基因沉默技术的研究进展,植物遗传资源学报,7(1):100-105)
Zheng S.J.,Snoeren T.A.,Hogewoning S.W.,VanLoon J.J.,and Dicke M.,2010,Disruption of plant carotenoid biosynthesis through virus-induced gene silencing affects oviposition behaviour of the butterfly Pieris rapae,New Phytol.,186(3):733-745
Zhou X.F.,Sun J.D.,Zhao Z.,Lv J.,Wei X.W.,Cai R.,and Xu H.W.,2010,The feasibility analysis of PVX and TRV vectors as the VIGS tool for studying the gene function,Physics Procedia,33:46-54