三种工艺提取丹参有效成分及丹酚酸B分离纯化研究
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摘要
目的:建立同时提取丹参中丹参酮IIA与丹酚酸B两种主要活性成分的方法及丹酚酸B的纯化工艺。方法:以丹参酮IIA与丹酚酸B的提取率和药效实验中血液流变参数为评价指标,考察最佳提取方法;并进一步用大孔树脂优选出丹酚酸B的最优分离纯化工艺。结果:3种提取方法中,SFE-CO2萃取法对丹参酮IIA的提取率明显高于乙醇超声提取;3种提取液都具有较好的改善血液流变学的作用,组间比较,乙醇超声提取和SFE-CO2萃取无显著性差异,但均明显优于不同浓度乙醇提取。丹参中丹酚酸B的分离、纯化选用SIPI905大孔树脂,丹酚酸B的最大上样量(即比吸附量)为9.20mg/g,洗脱剂为20%乙醇,用量为150mL,总固形物得率为5.56%,丹酚酸B得量占固体物量的64.98%。结论:综合3种提取方法的提取率以及药效实验结果,考虑降低成本、简化工艺和质量稳定,宜采用乙醇超声提取;SIPI905大孔树脂具备的优良特性适用于丹参中丹酚酸B的分离、纯化。
Objective:To establish a method for simultaneous extraction of tanshinone IIA and salvianolic acid B from Salvia miltiorrhiza and the purification process of salvianolic acid B.Methods:Taking the extraction rate of tanshinone IIA and salvianolic acid B and the hemorheological parameters in the pharmacodynamic experiment as the evaluation indexes,the optimum extraction method was investigated,and the optimum separation and purification process of salvianolic acid B was optimized by macroporous resin.Results:The extraction rate of SFE-CO2 was significantly higher than that of ethanol ultrasonic extraction in extracting tanshinone IIA,and the three extracts had better effect on improving hemorheology.There was no significant difference between groups in ethanol ultrasonic extraction and SFE-CO2 extraction,but they were obviously superior to ethanol extraction of different concentrations.Salvianolic acid B in Salvia miltiorrhiza was separated and purified by SIPI905 macroporous resin.The maximum sample loading(i.e.specific adsorption capacity)of salvianolic acid B was 9.20 mg/g,eluent was 20%ethanol and the dosage was 150 mL.The total solid yield was 5.56%,and salvianolic acid B accounted for 64.98%of the solid content.Conclusion:Ethanol ultrasonic extraction is suitable for the three extraction methods,considering the cost reduction,simplified process and stable quality,by synthesizing the extraction rate and pharmacodynamic test results of the three extraction methods.SIPI905 macroporous resin has excellent properties and is suitable for the separation and purification of salvianolic acid B from Salvia miltiorrhiza.
Objective:To establish a method for simultaneous extraction of tanshinone IIA and salvianolic acid B from Salvia miltiorrhiza and the purification process of salvianolic acid B.Methods:Taking the extraction rate of tanshinone IIA and salvianolic acid B and the hemorheological parameters in the pharmacodynamic experiment as the evaluation indexes,the optimum extraction method was investigated,and the optimum separation and purification process of salvianolic acid B was optimized by macroporous resin.Results:The extraction rate of SFE-CO2 was significantly higher than that of ethanol ultrasonic extraction in extracting tanshinone IIA,and the three extracts had better effect on improving hemorheology.There was no significant difference between groups in ethanol ultrasonic extraction and SFE-CO2 extraction,but they were obviously superior to ethanol extraction of different concentrations.Salvianolic acid B in Salvia miltiorrhiza was separated and purified by SIPI905 macroporous resin.The maximum sample loading(i.e.specific adsorption capacity)of salvianolic acid B was 9.20 mg/g,eluent was 20%ethanol and the dosage was 150 mL.The total solid yield was 5.56%,and salvianolic acid B accounted for 64.98%of the solid content.Conclusion:Ethanol ultrasonic extraction is suitable for the three extraction methods,considering the cost reduction,simplified process and stable quality,by synthesizing the extraction rate and pharmacodynamic test results of the three extraction methods.SIPI905 macroporous resin has excellent properties and is suitable for the separation and purification of salvianolic acid B from Salvia miltiorrhiza.
引文
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[2]杨志霞,林谦,马利.丹参对心血管疾病药理作用的文献研究[J].世界中西医结合杂志,2012,7(2):93-96.
[3]赵志芳.丹参对心血管疾病治疗的研究进展[J].海峡药学,2016,28(5):41-44.
[4]王艳梅,曹俊岭.丹参中酚酸类化合物的化学和药理研究进展[J].世界中医药,2016,11(6):1126-1130.
[5]杨蝉,杨玉琴,张丽艳,等.降粘胶囊中丹参酮ⅡA和丹酚酸B的含量测定[J].时珍国医国药,2014,25(6):1354-1356.
[6]吴涓,田璐璐,祝德秋.大孔树脂在中药分离纯化中的应用进展[J].上海医药,2013,34(19):46-49.
[7]刘春憬.大孔吸附树脂在中药分离纯化中的研究进展[J].农产品加工,2015(4):54-57.
[8]陈佳亮.大孔吸附树脂在中药分离纯化应用中影响因素研究[D].北京:北京工业大学,2012.
[9]国家食品药品监督管理局信息中心.动植物药有效成分提取分离和药理活性筛选汇编[R].北京:国家食品药品监督管理局信息中心,2004.
[10]寇俊萍,裴兴辰,余伯阳,等.生脉散总皂苷对实验性心肌缺氧保护作用的研究[J].中华中医药学刊,2004,22(5):822-824.
[11]尤燕,寇俊萍,余伯阳,等.复方丹参片合生脉饮抗小鼠心肌缺氧缺血作用研究[J].中药药理与临床,2007(5):4-7.
[12]张慧杰.中药小复方对急性心肌缺血大鼠CK-MB含量和血流动力学影响的实验研究[D].长春:长春中医药大学,2016.
[13]李伟霞,黄美艳,唐于平,等.大鼠急性血瘀模型造模方法的研究与评价[J].中国药理学通报,2011,27(12):1761-1765.
[14]闫俊,冯娟,杨雪,等.丹参酮ⅡA的药理作用及疾病治疗的最新进展[J].实用药物与临床,2015(8):972-977.