濒危植物四药门花的组培快繁
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摘要
以濒危植物四药门花茎段为外植体,研究了四药门花组培快繁体系,为四药门花的繁殖保护提供了科学依据。结果表明:(1)茎段外植体使用0.1%HgCl_2灭菌、灭菌时间10 min时,成功率最高(90.59%)。(2)茎段外植体叶腋芽诱导以MS+6-BA 1.0 mg·L~(-1)+IBA 0.2 mg·L~(-1)为最佳的诱导培养基。(3)最佳的增殖培养基为1/4MS+6-BA 1.0 mg·L~(-1)+KT 2.0 mg·L~(-1)+IBA 0.2 mg·L~(-1)+AC 0.3 g·L~(-1)。(4)最佳生根培养基为WPM+IBA 1.0 mg·L~(-1)。(5)组培苗最佳的移栽基质为进口泥炭土,30 d时存活率(100%)、苗高(6.24 cm)与冠幅(29.29 cm~2)均为最大。
To provide a scientific basis for the reproduction and protection of this endangered plant,complete tissue culture and rapid propagation system of Loropetalum subcordatum was studied with the young stem segment as explants.There were five main results of this research:(1)Stem explants were sterilized with 0.1%HgCl_2,the best sterilization time was 10 minutes,and the highest success rate was 90.59%.(2)MS+6-BA 1.0mg·L~(-1)+IBA 0.2 mg·L~(-1 )was the best inducing culture.(3)The best proliferation medium was 1/4MS+6-BA 1.0mg·L~(-1)+KT 2.0 mg·L~(-1)+IBA 0.2 mg·L~(-1)+AC 0.3 g·L~(-1).(4)The best rooting medium was WPM+IBA 1.0 mg·L~(-1).(5)The best transplanting substrate for tissue culture plantlets was imported peat soil.The survival rate(100%),seedling height(6.24 cm)and crown width(29.29 cm~2)were the highest on the 30th day.
To provide a scientific basis for the reproduction and protection of this endangered plant,complete tissue culture and rapid propagation system of Loropetalum subcordatum was studied with the young stem segment as explants.There were five main results of this research:(1)Stem explants were sterilized with 0.1%HgCl_2,the best sterilization time was 10 minutes,and the highest success rate was 90.59%.(2)MS+6-BA 1.0mg·L~(-1)+IBA 0.2 mg·L~(-1 )was the best inducing culture.(3)The best proliferation medium was 1/4MS+6-BA 1.0mg·L~(-1)+KT 2.0 mg·L~(-1)+IBA 0.2 mg·L~(-1)+AC 0.3 g·L~(-1).(4)The best rooting medium was WPM+IBA 1.0 mg·L~(-1).(5)The best transplanting substrate for tissue culture plantlets was imported peat soil.The survival rate(100%),seedling height(6.24 cm)and crown width(29.29 cm~2)were the highest on the 30th day.
引文
Chen XX,Shen CQ,Hong WJ,et al(2016).Distribution of Loropetalum subcordatum in Zhongshan Wuguishan,a plant species with extremely small populations.J Anhui Agric Sci,44(23):1-3(in Chinese with English abstract)[陈晓熹,申长青,洪文君等(2016).极小种群植物四药门花在中山五桂山的种群分布.安徽农业科学,44(23):1-3]
Deng GX,Yu H,Song PF,et al(2011).Effect of different basic media on proliferation and growth of clumpy shoot of southern highbush blueberry(Vaccinium corymbosum hybrids).J Plant Resour Environ,20(1):60-64(in Chinese with English abstract)[邓桂秀,於虹,宋鹏飞等(2011).不同基本培养基对南方高丛蓝浆果丛生枝增殖及生长的影响.植物资源与环境学报,20(1):60-64]
He MK,Huang JX,Huang CT,et al(2013).Study on the propagation technique of Loropetalum subcordatum.Practical For Technol,(11):50-53(in Chinese)[何妙坤,黄久香,黄川腾等(2013).四药门花扦插繁殖技术研究.林业实用技术,(11):50-53]
Jiang ZQ,Li CZ,Yu L,et al(2018).Regulating effects of exogenous IBA on stem cutting and activity of related enzymes during rooting of Carya illinoensis.Jiangsu Agric Sci,46(7):152-154(in Chinese with English abstract)[姜宗庆,李成忠,余乐等(2018).外源IBA对薄壳山核桃嫩枝扦插及其生根过程中相关酶活性的调控效应.江苏农业科学,46(7):152-154]
Ma LN,He J,Li G(2018).Tissue culture and rapid propagation of Ziziphora bungeana Juz.Plant Physiol J,54(8):1349-1355(in Chinese with English abstract)[马丽娜,何江,李冠(2018).新塔花的组织培养与快速繁殖.植物生理学报,54(8):1349-1355]
Sun HM,Chen Y,Liang YX,et al(2015).Preliminary study on tissue culture of endangered plant L.subcordatum.Xiandai Hortic,(4):12-13(in Chinese)[孙红梅,陈彦,梁银兴等(2015).濒危植物四药门花的组织培养研究初探.现代园艺,(4):12-13]
Wang LP,Dong LF,Jia CX,et al(2008).Optimum alternate cycles and concent of activated carbon’s in test tube seedling cultivation of Chinese pine.J Northwest For Univ,23(5):79-83(in Chinese with English abstract)[王丽萍,董丽芬,贾彩霞等(2008).油松试管苗培养中活性炭最适交替周期及浓度的研究.西北林学院学报,23(5):79-83]
Yin MH,Nie FQ,Hu WT,et al(2011).Effects of KT and NAA on the proliferation and differentiation of Dendrobium candidum protocorm.Heilongjiang Agric Sci,(8):25-26(in Chinese with English abstract)[尹明华,聂凤琴,胡文韬等(2011).NAA和KT对铁皮石斛原球茎增殖与分化的影响.黑龙江农业科学,(8):25-26]
Yu YF(1999).A milestone in the protection of wild plants in China-National Key Protected Wild Plants List(1st Batch).Plants,(5):3(in Chinese)[于永福(1999).中国野生植物保护工作的里程碑--《国家重点保护野生植物名录(第一批)》出台.植物杂志,(5):3]
Zang WJ,Chen Y,Li Q,et al(2015).Tissue culture differentiation and callus induction of different explant from Pennisetum americanum×P.purureum.Pratac Sci,32(9):1451-1456(in Chinese with English abstract)[臧文静,陈莹,李青等(2015).杂交狼尾草不同外植体愈伤组织诱导.草业科学,32(9):1451-1456]
Zhang HD(2000).Higher Flora of China:Hamamelidaceae.Qingdao,Shandong:Qingdao Publishing House,3:710(in Chinese)[张宏达(2000).中国植物志:金缕梅科.山东青岛:青岛出版社,3:710]
Zhang YR,Peng YJ,Ma Q,et al(2016).Rapid propagation system of Catalpa bungei and C.fargesii Bur.f.duclouxii.J North Forestry Univ,44(11):5-9(in Chinese with English abstract)[张烨然,彭言劼,马勤等(2016).楸树与滇楸组培快繁技术.东北林业大学学报,44(11):5-9]
Zhao FQ,Yin Q,Hong WJ,et al(2017).Tissue culture and rapid propagation of Rhododendron moulmainense.Plant Physiol J,53(9):1666-1672(in Chinese with English abstract)[赵富群,尹茜,洪文君等(2017).毛棉杜鹃的组织培养与快速繁殖.植物生理学报,53(9):1666-1672]
Zhou L(2015).Effects of NAA and IBA on the rooting of Verbena bonariensis.J North Forestry Univ,30(5):161-164(in Chinese with English abstract)[周亮(2015).NAA和IBA生根剂对柳叶马鞭草插条生根的影响.西北林学院学报,30(5):161-164]
Deng GX,Yu H,Song PF,et al(2011).Effect of different basic media on proliferation and growth of clumpy shoot of southern highbush blueberry(Vaccinium corymbosum hybrids).J Plant Resour Environ,20(1):60-64(in Chinese with English abstract)[邓桂秀,於虹,宋鹏飞等(2011).不同基本培养基对南方高丛蓝浆果丛生枝增殖及生长的影响.植物资源与环境学报,20(1):60-64]
He MK,Huang JX,Huang CT,et al(2013).Study on the propagation technique of Loropetalum subcordatum.Practical For Technol,(11):50-53(in Chinese)[何妙坤,黄久香,黄川腾等(2013).四药门花扦插繁殖技术研究.林业实用技术,(11):50-53]
Jiang ZQ,Li CZ,Yu L,et al(2018).Regulating effects of exogenous IBA on stem cutting and activity of related enzymes during rooting of Carya illinoensis.Jiangsu Agric Sci,46(7):152-154(in Chinese with English abstract)[姜宗庆,李成忠,余乐等(2018).外源IBA对薄壳山核桃嫩枝扦插及其生根过程中相关酶活性的调控效应.江苏农业科学,46(7):152-154]
Ma LN,He J,Li G(2018).Tissue culture and rapid propagation of Ziziphora bungeana Juz.Plant Physiol J,54(8):1349-1355(in Chinese with English abstract)[马丽娜,何江,李冠(2018).新塔花的组织培养与快速繁殖.植物生理学报,54(8):1349-1355]
Sun HM,Chen Y,Liang YX,et al(2015).Preliminary study on tissue culture of endangered plant L.subcordatum.Xiandai Hortic,(4):12-13(in Chinese)[孙红梅,陈彦,梁银兴等(2015).濒危植物四药门花的组织培养研究初探.现代园艺,(4):12-13]
Wang LP,Dong LF,Jia CX,et al(2008).Optimum alternate cycles and concent of activated carbon’s in test tube seedling cultivation of Chinese pine.J Northwest For Univ,23(5):79-83(in Chinese with English abstract)[王丽萍,董丽芬,贾彩霞等(2008).油松试管苗培养中活性炭最适交替周期及浓度的研究.西北林学院学报,23(5):79-83]
Yin MH,Nie FQ,Hu WT,et al(2011).Effects of KT and NAA on the proliferation and differentiation of Dendrobium candidum protocorm.Heilongjiang Agric Sci,(8):25-26(in Chinese with English abstract)[尹明华,聂凤琴,胡文韬等(2011).NAA和KT对铁皮石斛原球茎增殖与分化的影响.黑龙江农业科学,(8):25-26]
Yu YF(1999).A milestone in the protection of wild plants in China-National Key Protected Wild Plants List(1st Batch).Plants,(5):3(in Chinese)[于永福(1999).中国野生植物保护工作的里程碑--《国家重点保护野生植物名录(第一批)》出台.植物杂志,(5):3]
Zang WJ,Chen Y,Li Q,et al(2015).Tissue culture differentiation and callus induction of different explant from Pennisetum americanum×P.purureum.Pratac Sci,32(9):1451-1456(in Chinese with English abstract)[臧文静,陈莹,李青等(2015).杂交狼尾草不同外植体愈伤组织诱导.草业科学,32(9):1451-1456]
Zhang HD(2000).Higher Flora of China:Hamamelidaceae.Qingdao,Shandong:Qingdao Publishing House,3:710(in Chinese)[张宏达(2000).中国植物志:金缕梅科.山东青岛:青岛出版社,3:710]
Zhang YR,Peng YJ,Ma Q,et al(2016).Rapid propagation system of Catalpa bungei and C.fargesii Bur.f.duclouxii.J North Forestry Univ,44(11):5-9(in Chinese with English abstract)[张烨然,彭言劼,马勤等(2016).楸树与滇楸组培快繁技术.东北林业大学学报,44(11):5-9]
Zhao FQ,Yin Q,Hong WJ,et al(2017).Tissue culture and rapid propagation of Rhododendron moulmainense.Plant Physiol J,53(9):1666-1672(in Chinese with English abstract)[赵富群,尹茜,洪文君等(2017).毛棉杜鹃的组织培养与快速繁殖.植物生理学报,53(9):1666-1672]
Zhou L(2015).Effects of NAA and IBA on the rooting of Verbena bonariensis.J North Forestry Univ,30(5):161-164(in Chinese with English abstract)[周亮(2015).NAA和IBA生根剂对柳叶马鞭草插条生根的影响.西北林学院学报,30(5):161-164]