摘要
目的探讨宫颈鳞状上皮病变中人乳头瘤病毒(HPV)L1蛋白与Rb相关基因蛋白的表达及意义。方法回顾性分析60例宫颈癌手术患者的临床资料,检测并分析人乳头瘤病毒L1蛋白与Rb相关基因蛋白与宫颈上皮内瘤变HPV16/18型感染、细胞学结果、组织病理学结果等的关系。结果 HPV L1蛋白、Rb相关基因蛋白与HPV亚型感染呈负相关(P<0.05);在细胞学方面,低分化细胞分型的HPV病毒感染患者HPV L1蛋白阳性率高于高分化细胞分型HPV病毒感染患者(如LSIL)(P<0.05);HPV感染患者中,低分化细胞分型的HPV病毒感染患者Rb蛋白阳性率高于高分化细胞分型HPV病毒感染患者(如LSIL)(P<0.05);在组织病理学方面,低度恶性的宫颈癌患者HPV L1蛋白阳性率高于高度恶性的宫颈上皮内瘤变患者(P<0.05);低度恶性的宫颈上皮内瘤变患者HPV L1蛋白阳性率高于高度恶性的宫颈上皮内瘤变患者(P<0.05)。结论 HPV L1蛋白的表达、Rb相关基因蛋白可能与宫颈病变程度呈负相关。HPV16/18感染的患者HPV L1蛋白、Rb相关基因蛋白的阳性率低于其他HPV亚型感染,HPV L1蛋白及Rb基因相关蛋白的阳性率在上皮内瘤变中表达率高于其他细胞亚型宫颈上皮内瘤变,且HPV L1蛋白及Rb基因相关蛋白的阳性率在宫颈上皮内瘤变(CIN)II及以上宫颈上皮内瘤变中较低。
Objective To investigate the expression and significance of human papillomavirus( HPV) L1 protein and Rb related gene protein in cervical squamous intraepithelial lesion.Methods The clinical data of 60 cases of patients with cervical cancer were analyzed retrospectively,and the relationship between human papillomavirus L1 protein and Rb related gene protein and cervical infection,cervical intraepithelial neoplasia of HPV16/18 infection,cytological findings and histopathological findings were detected and analyzed. Results There was negative correlation of HPV L1 protein,Rb related protein with HPV subtype infection( P < 0. 05); in terms of cytology,the positive rates of HPV and L1 protein in patients with poorly differentiated cell type HPV infection were significantly higher than those of the highly differentiated cell type HPV infected patients( such as LSIL)( P < 0. 05); in patients with HPV infection,the Rb protein positive rates of patients with poorly differentiated cell type HPV infection were significantly higher than those of highly differentiated cell type HPV infected patients( such as LSIL)( P < 0. 05); in terms of histopathology,the positive rates of HPV and L1 protein in patients with low-grade cervical cancer were significantly higher than those in highly malignant cervical cancer patients with cervical intraepithelial neoplasia( P < 0. 05); the positive rate of HPV and L1 protein in patients with cervical intraepithelial neoplasia in low-grade cervical cancer was higher than that in highly malignant cervical cancer patients with cervical intraepithelial neoplasia( P < 0. 05).Conclusions The expression of HPV L1 protein and Rb related gene protein may be negatively correlated with the degree of cervical lesions. The positive rates of HPV,L1 protein and Rb related gene protein in HPV16/18 infected patients are lower than those in other HPV subtypes,and the positive rates of HPV,L1 protein and Rb gene related protein in intraepithelial neoplasia are higher than those in other cell subtypes of cervical intraepithelial neoplasia. The positive rates of HPV,L1 protein and Rb gene related protein are lower in the cervical intraepithelial neoplasia( CIN) II and above cervical intraepithelial neoplasia.
引文
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