宫颈鳞状上皮病变中人乳头瘤病毒L1蛋白与Rb相关基因蛋白的表达及意义
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摘要
目的探讨宫颈鳞状上皮病变中人乳头瘤病毒(HPV)L1蛋白与Rb相关基因蛋白的表达及意义。方法回顾性分析60例宫颈癌手术患者的临床资料,检测并分析人乳头瘤病毒L1蛋白与Rb相关基因蛋白与宫颈上皮内瘤变HPV16/18型感染、细胞学结果、组织病理学结果等的关系。结果 HPV L1蛋白、Rb相关基因蛋白与HPV亚型感染呈负相关(P<0.05);在细胞学方面,低分化细胞分型的HPV病毒感染患者HPV L1蛋白阳性率高于高分化细胞分型HPV病毒感染患者(如LSIL)(P<0.05);HPV感染患者中,低分化细胞分型的HPV病毒感染患者Rb蛋白阳性率高于高分化细胞分型HPV病毒感染患者(如LSIL)(P<0.05);在组织病理学方面,低度恶性的宫颈癌患者HPV L1蛋白阳性率高于高度恶性的宫颈上皮内瘤变患者(P<0.05);低度恶性的宫颈上皮内瘤变患者HPV L1蛋白阳性率高于高度恶性的宫颈上皮内瘤变患者(P<0.05)。结论 HPV L1蛋白的表达、Rb相关基因蛋白可能与宫颈病变程度呈负相关。HPV16/18感染的患者HPV L1蛋白、Rb相关基因蛋白的阳性率低于其他HPV亚型感染,HPV L1蛋白及Rb基因相关蛋白的阳性率在上皮内瘤变中表达率高于其他细胞亚型宫颈上皮内瘤变,且HPV L1蛋白及Rb基因相关蛋白的阳性率在宫颈上皮内瘤变(CIN)II及以上宫颈上皮内瘤变中较低。
Objective To investigate the expression and significance of human papillomavirus( HPV) L1 protein and Rb related gene protein in cervical squamous intraepithelial lesion.Methods The clinical data of 60 cases of patients with cervical cancer were analyzed retrospectively,and the relationship between human papillomavirus L1 protein and Rb related gene protein and cervical infection,cervical intraepithelial neoplasia of HPV16/18 infection,cytological findings and histopathological findings were detected and analyzed. Results There was negative correlation of HPV L1 protein,Rb related protein with HPV subtype infection( P < 0. 05); in terms of cytology,the positive rates of HPV and L1 protein in patients with poorly differentiated cell type HPV infection were significantly higher than those of the highly differentiated cell type HPV infected patients( such as LSIL)( P < 0. 05); in patients with HPV infection,the Rb protein positive rates of patients with poorly differentiated cell type HPV infection were significantly higher than those of highly differentiated cell type HPV infected patients( such as LSIL)( P < 0. 05); in terms of histopathology,the positive rates of HPV and L1 protein in patients with low-grade cervical cancer were significantly higher than those in highly malignant cervical cancer patients with cervical intraepithelial neoplasia( P < 0. 05); the positive rate of HPV and L1 protein in patients with cervical intraepithelial neoplasia in low-grade cervical cancer was higher than that in highly malignant cervical cancer patients with cervical intraepithelial neoplasia( P < 0. 05).Conclusions The expression of HPV L1 protein and Rb related gene protein may be negatively correlated with the degree of cervical lesions. The positive rates of HPV,L1 protein and Rb related gene protein in HPV16/18 infected patients are lower than those in other HPV subtypes,and the positive rates of HPV,L1 protein and Rb gene related protein in intraepithelial neoplasia are higher than those in other cell subtypes of cervical intraepithelial neoplasia. The positive rates of HPV,L1 protein and Rb gene related protein are lower in the cervical intraepithelial neoplasia( CIN) II and above cervical intraepithelial neoplasia.
Objective To investigate the expression and significance of human papillomavirus( HPV) L1 protein and Rb related gene protein in cervical squamous intraepithelial lesion.Methods The clinical data of 60 cases of patients with cervical cancer were analyzed retrospectively,and the relationship between human papillomavirus L1 protein and Rb related gene protein and cervical infection,cervical intraepithelial neoplasia of HPV16/18 infection,cytological findings and histopathological findings were detected and analyzed. Results There was negative correlation of HPV L1 protein,Rb related protein with HPV subtype infection( P < 0. 05); in terms of cytology,the positive rates of HPV and L1 protein in patients with poorly differentiated cell type HPV infection were significantly higher than those of the highly differentiated cell type HPV infected patients( such as LSIL)( P < 0. 05); in patients with HPV infection,the Rb protein positive rates of patients with poorly differentiated cell type HPV infection were significantly higher than those of highly differentiated cell type HPV infected patients( such as LSIL)( P < 0. 05); in terms of histopathology,the positive rates of HPV and L1 protein in patients with low-grade cervical cancer were significantly higher than those in highly malignant cervical cancer patients with cervical intraepithelial neoplasia( P < 0. 05); the positive rate of HPV and L1 protein in patients with cervical intraepithelial neoplasia in low-grade cervical cancer was higher than that in highly malignant cervical cancer patients with cervical intraepithelial neoplasia( P < 0. 05).Conclusions The expression of HPV L1 protein and Rb related gene protein may be negatively correlated with the degree of cervical lesions. The positive rates of HPV,L1 protein and Rb related gene protein in HPV16/18 infected patients are lower than those in other HPV subtypes,and the positive rates of HPV,L1 protein and Rb gene related protein in intraepithelial neoplasia are higher than those in other cell subtypes of cervical intraepithelial neoplasia. The positive rates of HPV,L1 protein and Rb gene related protein are lower in the cervical intraepithelial neoplasia( CIN) II and above cervical intraepithelial neoplasia.
引文
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[12]李玮,吕杰强,鲁意.人乳头状瘤病毒混合感染对高度鳞状上皮内病变和宫颈癌的风险研究[J].中华实验和临床病毒学杂志,2015,29(1):41-43.DOI:10.3760/cma.j.issn.1003-9279.2015.01.014.
[13]冯晓贺,王仁辉,徐秋杰,等.人乳头瘤病毒L1壳蛋白检测在宫颈鳞状上皮内病变中的表达及临床意义[J].中国优生与遗传杂志,2015,35(4):27-28.DOI:10.13404/j.cnki.cjbhh.2015.04.012.
[14]王娟,李燕,赵骏达,等.人乳头状瘤病毒L1壳蛋白和人端粒酶RNA基因联合检测在宫颈癌筛查中的意义[J].中国医药导报,2015,12(17):60-63.
[15]梁丽霞,肖燕,高玮.HPV L1壳蛋白表达与宫颈上皮瘤变及HPV分型的关系[J].国际检验医学杂志,2016,37(14):1944-1945.DOI:10.3969/j.issn.1673-4130.2016.14.017.
[16]苏光,唐振利,张凡,等.HPVL1蛋白和HMGA1在子宫颈病变中的表达及临床意义[J].中国妇产科临床杂志,2016,17(4):319-322.DOI:10.13390/j.issn.1672-1861.2016.04.010.
[17]袁玉亮.基因重组HPV16L1蛋白的血清学实验在人乳头瘤病毒16感染诊断中的应用价值[J].中国妇幼保健,2016,31(16):3387-3389.DOI:10.7620/zgfybj.j.issn.1001-4411.2016.16.71.
[2]Xiao W,Bian M,Ma L,et al.Immunochemical analysis of human papillomavirus L1 capsid protein in liquid-based cytology samples from cervical lesions[J].Acta Cytol,2016,54(5):661-667.DOI:10.1159/000325229.
[3]Nayar R,Wilber DC.The bethesda system for reporting cervical cytology.Definitions,Criteria,and Explanatory Notes,3nded[M].New York,NY:Springer-Verlag,2015.
[4]Tavassoli FA,Devilee P.WHO classification of tumors.Pathologyand genetics of tumors of the breast and female genital organs[M].Lyon:IARC Press,2003,266-272.DOI:10.1016/j.bulcan.2017.08.
[5]潘琼慧,黄凌霄,陈育梅,等.P16INK4A、L1壳蛋白联合检测在宫颈鳞状上皮内病变中的应用研究进展[J].实用医学杂志,2015,31(2):324-326.DOI:10.3969/j.issn.1006-5725.2015.02.057.
[6]高宝莲,钱萍,马祯一,等.宫颈鳞状上皮病变中人乳头瘤病毒L1蛋白与P16、Ki-67的表达及意义[J].中国妇幼保健,2015,30(7):1109-1111.DOI:10.7620/zgfybj.j.issn.1001-4411.2015.07.48.
[7]潘琼慧,郑翔,朱雪燕,等.人乳头瘤病毒L1壳蛋白在筛查宫颈鳞状上皮内病变中的应用[J].现代妇产科进展,2016,25(11):808-812.DOI:10.13283/j.cnki.xdfckjz.2016.11.002.
[8]沈姚琴,赖娟,贺俊霞.HPV L1壳蛋白联合HPV分型、TCT检测在宫颈病变诊治中的临床应用研究[J].中国医师杂志,2015,17(3):365-368.DOI:10.3760/cma.j.issn.1008-1372.2015.03.013.
[9]王丽凤,韩蓉晖,李丽,等.高危型人乳头瘤病毒感染的子宫颈鳞状上皮组织中p16INK4α蛋白表达及其意义[J].肿瘤研究与临床,2016,28(11):757-760.DOI:10.3760/cma.j.issn.1006-9801.2016.11.009.
[10]王璐,王晨阳,王武亮,等.高危型人乳头瘤病毒E_6/E_7 mRNA检测对宫颈高度鳞状上皮内病变诊断及随访价值研究[J].中国实用妇科与产科杂志,2016(6):580-582.
[11]何鑫,陶绘丞,王淑珍,等.高危型人乳头瘤病毒阴性/不典型鳞状细胞和低度鳞状上皮内病变患者的随访观察[J].首都医科大学学报,2015,36(2):205-211.DOI:10.3969/j.issn.1006-7795.2015.02.009.
[12]李玮,吕杰强,鲁意.人乳头状瘤病毒混合感染对高度鳞状上皮内病变和宫颈癌的风险研究[J].中华实验和临床病毒学杂志,2015,29(1):41-43.DOI:10.3760/cma.j.issn.1003-9279.2015.01.014.
[13]冯晓贺,王仁辉,徐秋杰,等.人乳头瘤病毒L1壳蛋白检测在宫颈鳞状上皮内病变中的表达及临床意义[J].中国优生与遗传杂志,2015,35(4):27-28.DOI:10.13404/j.cnki.cjbhh.2015.04.012.
[14]王娟,李燕,赵骏达,等.人乳头状瘤病毒L1壳蛋白和人端粒酶RNA基因联合检测在宫颈癌筛查中的意义[J].中国医药导报,2015,12(17):60-63.
[15]梁丽霞,肖燕,高玮.HPV L1壳蛋白表达与宫颈上皮瘤变及HPV分型的关系[J].国际检验医学杂志,2016,37(14):1944-1945.DOI:10.3969/j.issn.1673-4130.2016.14.017.
[16]苏光,唐振利,张凡,等.HPVL1蛋白和HMGA1在子宫颈病变中的表达及临床意义[J].中国妇产科临床杂志,2016,17(4):319-322.DOI:10.13390/j.issn.1672-1861.2016.04.010.
[17]袁玉亮.基因重组HPV16L1蛋白的血清学实验在人乳头瘤病毒16感染诊断中的应用价值[J].中国妇幼保健,2016,31(16):3387-3389.DOI:10.7620/zgfybj.j.issn.1001-4411.2016.16.71.