乳腺癌患者血清趋化因子配体8、白细胞介素1-β、sCD200的表达水平及临床意义
摘要
目的探讨乳腺癌患者血清趋化因子配体8 (CXCL8)、白细胞介素1-β(IL-1β)、sCD200的表达水平及临床意义,为临床诊断与治疗乳腺癌提供参考依据。方法选取2015年6月-2017年3月上海交通大学医学院附属第九人民医院收治的80例乳腺癌患者及40例乳腺良性增生患者为研究对象,选取同期40例女性体检健康者为对照组;通过流式细胞仪检测T淋巴细胞亚群CD3~+、CD4~+、CD8~+、CD4~+/CD8~+水平,全自动特定蛋白分析仪检测免疫球蛋白IgA、IgG水平以及酶联免疫吸附法(ELISA)检测血清CXCL8、IL-1β、sCD200表达水平。结果乳腺癌组CD3~+、CD4~+、CD4~+/CD8~+水平显著低于乳腺良性增生组与对照组,而CD8~+、IgG水平高于乳腺良性增生组与对照组,差异有统计学意义(均P<0. 01);乳腺良性增生组CD3~+、CD4~+、CD4~+/CD8~+水平显著低于对照组,差异有统计学意义(均P<0. 05),而CD8~+、IgG水平均显著高于对照组,差异有统计学意义(均P<0. 01); 3组IgA水平比较差异无统计学意义(均P>0. 05)。乳腺癌组及乳腺良性增生组CXCL8、IL-1β、sCD200表达水平显著高于对照组,且乳腺癌组显著高于乳腺良性增生组,差异有统计学意义(均P<0. 01)。乳腺癌Ⅲ期、Ⅳ期CXCL8、IL-1β、sCD200表达水平显著高于Ⅰ期、Ⅱ期,差异有统计学意义(均P<0. 01),而Ⅰ期与Ⅱ期、Ⅲ期与Ⅳ期比较差异无统计学意义(均P>0. 05)。乳腺癌区域淋巴结转移者CXCL8、IL-1β、sCD200表达水平显著高于无区域淋巴结转移者,远处转移者CXCL8、IL-1β、sCD200表达水平显著高于无远处转移者,差异有统计学意义(均P<0. 01)。结论乳腺癌患者机体处于较低免疫功能状态,血清CXCL8、IL-1β、sCD200表达水平显著升高,推测三者可作为诊断乳腺癌病程的参考指标。
引文
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[22]侯睿达,安治国,刘俊婷,等.CD200和CD200R在膀胱和肾脏肿瘤表达的研究[J].中国实验诊断学,2016,20(10):1661-1663.
[2]孙治君.乳腺癌治疗现状[J].重庆医学,2010,39(7):772-773.
[3]王先明,吴家豪,王敏,等.从分子生物学特征探讨乳腺癌个体化治疗策略[J].中华普通外科学文献:电子版,2009,3(4):46-48.
[4]中国抗癌协会乳腺癌专业委员会.中国抗癌协会乳腺癌诊治指南与规范[J].中国癌症杂志,2015,25(9):692-754.
[5]Frederick LGMD,David LPMD,Irvin DFMD,et al.AJCC Cancer Staging Manual[J].AJCC cancer staging manual,Springer,2010,83(4):803-819.
[6]申耀宗.乳腺增生症的诊断与处理[J].中国医师进修杂志,2001,24(9):9-10.
[7]徐雅莉,孙强,单广良,等.中国女性乳腺癌发病相关危险因素:病例对照研究[J].协和医学杂志,2011,2(1):7-14.
[8]范丽梅,柳昀熠,潘钰,等.炎症与肿瘤的关系研究进展[J].江汉大学学报(自然科学版),2016,44(5):432-437.
[9]吕松林.细胞免疫功能检测对乳腺癌临床分期及预后的评估价值[J].中国现代药物应用,2016,10(1):29-30.
[10]Belperio JA,Keane MP,Arenberg DA,et al.CXC chemokines in angiogenesis[J].J Leukoc Biol,2000,68(1):1-8.
[11]Tanaka T,Bai Z,Srinoulprasert Y,et al.Chemokines in tumor progression and metastasis[J].Cancer Science,2005,96(6):317-322.
[12]Jeong E,Koo JE,Yeon SH,et al.PPARδdeficiency disrupts hypoxia-mediated tumorigenic potential of colon cancer cells[J].Molecular Carcinogenesis,2014,53(11):926-937.
[13]Katoh H,Wang D,Daikoku T,et al.CXCR2-Expressing Myeloid-Derived Suppressor Cells Are Essential to Promote Colitis-Associated Tumorigenesis[J].Cancer Cell,2013,24(5):631-644.
[14]Benoy IH,Salgado R,Van DP,et al.Increased serum interleukin-8 in patients with early and metastatic breast cancer correlates with early dissemination and survival[J].Clin Cancer Res,2004,10(21):7157-7162.
[15]景彩萍,何静子,魏晓丽,等.炎症因子IL-1β、IL-8、TNF-α与乳腺癌的关系[J].延安大学学报:医学科学版,2014,12(3):61-63.
[16]Voronov E,Dotan S,Krelin Y,et al.Unique Versus Redundant Functions of IL-1αand IL-1βin the Tumor Microenvironment[J].Frontiers in Immunology,2013,4(10):177-182.
[17]Carmi Y,Dotan S,Rider P,et al.The Role of IL-1βin the Early Tumor Cell-Induced Angiogenic Response[J].Journal of Immunology,2013,190(7):3500-3509.
[18]Suswam EA,Nabors LB,Huang Y,et al.IL-1beta induces stabilization of IL-8 mRNA in malignant breast cancer cells via the 3'untranslated region:Involvement of divergent RNA-binding factors HuR,KSRP and TIAR[J].International Journal of Cancer Journal International Du Cancer,2005,113(6):911-919.
[19]凌存保,吴隽松.乳腺癌患者血清IL-6、IL-1β、TNF-α的变化及其临床意义[J].标记免疫分析与临床,2014,21(5):532-534.
[20]Gorczynski RM,Yu K,Clark D,et al.Receptor engagement on cells expressing a ligand for the tolerance-inducing molecule OX2induces an immunoregulatory population that inhibits alloreactivity in vitro and in vivo[J].Journal of Immunology,2000,165(9):4854-4860.
[21]Fatemeh T,Liu JQ,Liu Z,et al.Melanoma Cell Expression of CD200Inhibits Tumor Formation and Lung Metastasis via Inhibition of Myeloid Cell Functions[J].Plos One,2012,7(2):e31442.
[22]侯睿达,安治国,刘俊婷,等.CD200和CD200R在膀胱和肾脏肿瘤表达的研究[J].中国实验诊断学,2016,20(10):1661-1663.