蒙药复方述达格-4特征图谱初步分析
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摘要
目的:分析蒙药复方述达格-4的特征图谱。方法:采用高效液相色谱法。色谱柱为Agilent Extend C18,流动相为0.4%乙酸溶液-甲醇(梯度洗脱),流速为0.6 m L/min,检测波长为286 nm,柱温为30℃,进样量为20μL。分别对复方述达格-4全方及各单味药体内外特征图谱进行分析和对比。结果:复方述达格-4全方体外特征图谱有47个峰;其体内特征图谱有24个峰,其中峰1、4、7～9、12、14、18～20、22、25、38～41、44、46对应的18个成分为体外直接入血成分;各单味药体内特征图谱中峰1、4、7、8、12、14、18～22、25、40、46来源于高良姜药材;峰14、18、19、22、44来源于木香药材;峰1、4、7～9、12、14、18、19、22来源于石菖蒲药材。结论:直接入血成分及代谢产物可能为复方述达格-4在体内发挥作用的有效成分;该方法可用于其特征图谱的分析。
OBJECTIVE:To analyse characteristic chromatograms of Mongolian Compound Shudage-4. METHODS:HPLC method was adopted. The determination was performed on Agilent Extend C18 with mobile phase consisted of 0.4% acetic acid solution-methanol(gradient elution) at the flow rate of 0.6 mL/min. The detection wavelength was set at 286 nm,and column temperature was 30 ℃. The sample size was 20 μL. The in vivo and in vitro characteristic chromatograms of allsquare of Compound Shudage-4 and single ingredients were analysed and compared respectively. RESULTS:Totally 47 peaks were identified from in vitro characteristic chromatograms of allsquare of Compound Shudage-4. There were 24 common peaks from in vivo characteristic chromatograms,among which 18 ingredients corresponding to common peaks 1,4,7-9,12,14,18-20,22,25,38-41,44,46 were direct blood components in vitro;peaks 1,4,7,8,12,14,18-22,25,40 and 46 of serum test sample were from Alpinia officinarum;peaks 14,18,19,22,44 were from Aucklandia lappa;peaks 1,4,7-9,12,14,18,19,22 were from Acorus tatarinowii. CONCLUSIONS:Blood components and some metabolites may beallsquare of Compound Shudage-4. The method can be used for the analysis of characteristic chromatograons of it.
OBJECTIVE:To analyse characteristic chromatograms of Mongolian Compound Shudage-4. METHODS:HPLC method was adopted. The determination was performed on Agilent Extend C18 with mobile phase consisted of 0.4% acetic acid solution-methanol(gradient elution) at the flow rate of 0.6 mL/min. The detection wavelength was set at 286 nm,and column temperature was 30 ℃. The sample size was 20 μL. The in vivo and in vitro characteristic chromatograms of allsquare of Compound Shudage-4 and single ingredients were analysed and compared respectively. RESULTS:Totally 47 peaks were identified from in vitro characteristic chromatograms of allsquare of Compound Shudage-4. There were 24 common peaks from in vivo characteristic chromatograms,among which 18 ingredients corresponding to common peaks 1,4,7-9,12,14,18-20,22,25,38-41,44,46 were direct blood components in vitro;peaks 1,4,7,8,12,14,18-22,25,40 and 46 of serum test sample were from Alpinia officinarum;peaks 14,18,19,22,44 were from Aucklandia lappa;peaks 1,4,7-9,12,14,18,19,22 were from Acorus tatarinowii. CONCLUSIONS:Blood components and some metabolites may beallsquare of Compound Shudage-4. The method can be used for the analysis of characteristic chromatograons of it.
引文
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[2]张灵娜,林兵,宋洪涛.中药血清药理学、血清药物化学的研究概况及展望[J].中草药,2015,46(17):2662-2665.
[3]马飞祥,薛培凤,王媛媛,等.中药血清药物化学研究进展[J].中国中药杂志,2017,42(7):1265-1270.
[4]国家药典委员会.中华人民共和国卫生部药品标准:蒙药分册[S].呼和浩特:内蒙古科学技术出版社,1998:158.
[5]杨洋,贾鑫,董玉,等.蒙药述达格-4的化学成分研究[J].内蒙古大学学报(自然科学版),2013,44(4):392-396.
[6]信莎莎,杜银飞,贾鑫,等.蒙药复方述达格-4化学成分的分离与结构鉴定[J].北京中医药大学学报,2016,39(11):945-948.
[7]信莎莎,杨洋,白埔,等.蒙药述达格-4抗小鼠胃溃疡的作用研究[J].北京中医药大学学报,2015,38(8):524-528.
[8]贾鑫,信莎莎,杨洋,等.蒙药复方述达格-4抗实验性胃溃疡的作用研究[J].内蒙古医科大学学报,2013,35(5):381-384.
[9]信莎莎,杨洋,孙国园,等.HPLC法测定蒙药复方述达格-4中1-苯基-7-(3-甲氧基-4-羟基)苯基-5-醇-3-庚酮的含量[J].内蒙古大学学报(自然科学版),2015,46(2):191-195.
[10]雷露静.蒙药三子散质量控制及血清药物化学初步研究[D].呼和浩特:内蒙古医科大学,2016.