尼克酸治疗恶性黑素瘤的相关机制研究
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摘要
目的探讨尼克酸在恶性黑素瘤的治疗前景及相关分子机制方法通过Transwell体外迁移实验,观察尼克酸对B16-F10黑色素瘤细胞迁移是否有抑制作用,以及不同浓度尼克酸对黑素瘤细胞的影响。应用RT-PCR技术检测转录因子snail2、twist及粘附因子E-cardherin的表达。结果通过Transwell体外迁移实验我们发现,尼克酸在体外对B16-F10黑色素瘤细胞的迁移有明显的抑制作用,且呈浓度依耐关系,尼克酸可促进肿瘤细胞迁移的相关基因snail2和twist表达明显减少(P <0.01),而抑制肿瘤细胞迁移相关基因E-cardherin明显增加。结论尼克酸在体外通能抑制黑素瘤细胞的迁移,对其治疗提供一定的理论基础。
Objective To explore the prospect and mechanism of niacin in the treatment of malignant melanoma. Methods Through transwell migration experiment in vitro,the migration inhibition effect of niacin on B16-F10 melanoma cells was observed,as well as the effect of different concentrations of niacin on melanoma cells. The expression of transcription factor snail2, twist and adhesive factor E-cardherin was detected by RT-PCR. Results Through transwell in vitro migration experiments, we found that niacin significantly inhibits the migration of B16-F10 melanoma cells, and shows a concentration-dependent relationship. Niacin can significantly reduce the expression of snail2 and twist related genes in tumor cell migration(P < 0.01), while E-cardherin inhibiting tumor cell migration was significantly increased. Conclusion Niacin can inhibit the migration of melanoma cells in vitro and provide theoretical basis for its treatment.
Objective To explore the prospect and mechanism of niacin in the treatment of malignant melanoma. Methods Through transwell migration experiment in vitro,the migration inhibition effect of niacin on B16-F10 melanoma cells was observed,as well as the effect of different concentrations of niacin on melanoma cells. The expression of transcription factor snail2, twist and adhesive factor E-cardherin was detected by RT-PCR. Results Through transwell in vitro migration experiments, we found that niacin significantly inhibits the migration of B16-F10 melanoma cells, and shows a concentration-dependent relationship. Niacin can significantly reduce the expression of snail2 and twist related genes in tumor cell migration(P < 0.01), while E-cardherin inhibiting tumor cell migration was significantly increased. Conclusion Niacin can inhibit the migration of melanoma cells in vitro and provide theoretical basis for its treatment.
引文
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[3]Hay,E.An overview of epithelio-mesenchymal transformation[R].Cells Tissues Organs,1995,154(1),8-20.DOI:10.1159/000147748.
[4]Samy Lamouille,Jian Xu,Rik Derynck.Molecular mechanisms of epithelial-mesenchymal transition[J].Nat Rev Mol Cell Biol,2014,15(3):178-196.DOI:10.1038/nrm3758
[5]Xin Ye,Robert A,Weinberg.Epithelial-Mesenchymal Plasticity:A central regulator of cancer progression[J].Trends Cell Biol,2015,25(11):675-686.DOI:10.1016/j.tcb.2015.07.012
[6]Bing Tian,Yingxin Zhao,Hong Sun,et al.BRD4mediates NF-κB-dependent epithelial-mesenchymal transition and pulmonary fibrosis via transcriptional elongation[J].Am J Physiol Lung Cell Mol Physiol,2 0 1 6,3 1 1(6):L 1 1 8 3-L 1 2 0 1.D O I:1 0.1 1 5 2/ajplung.00224.2016
[7]Raghu Kalluri,Robert A,Weinberg.The basics of epithelial-mesenchymal transition[J].J Clin Invest,2009,119(6):1420-1428.DOI:10.1172/JCI39104
[8]HervéAcloque,Meghan S.Adams,et al.Epithelialmesenchymal transitions:the importance of changing cell state in development and disease[J].Angela Nieto J Clin Invest,2009,119(6):1438-1449.DOI:10.1172/JCI38019
[9]Sendurai A.Mani,Wenjun Guo,Mai-Jing Liao,et al.The epithelial-mesenchymal transition generates cells with properties of stem cells[J].Cell,2008,133(4):704-715.DOI:10.1016/j.cell.2008.03.027
[10]Kornelia Polyak,Robert A,Weinberg.Transitions between epithelial and mesenchymal states:acquisition of malignant and stem cell traits[R].Nature Reviews Cancer,2009,9(4):265-273.DOI:10.1038/nrc2620
[11]Kuphal S,Bosserhoff AK.E-cadherin cell-cell communication in melanogenesis and during development of malignant melanoma[J].Arch Biochem Biophys,2012,524(1):43-47.DOI:10.1016/j.abb.2011.10.020
[12]Wang JX,Fukunaga-Kalabis M,Herlyn M.Crosstalk in skin:melanocytes,keratinocytes,stemcells,and melanoma[J].J Cell Commun Signal,201,10(3):191-196.DOI:10.1007/s12079-016-0349-3