湘西山莓叶挥发油化学成分及其抗氧化、抑菌活性研究
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摘要
目的:研究湘西山莓叶挥发油化学成分,及其抗氧化活性、抑菌作用,为山莓叶挥发油的进一步开发利用提供基础依据。方法:采用水蒸气蒸馏法提取山莓叶挥发油,通过气相色谱-质谱联用(GC-MS)技术确定其中化学成分与相对百分含量。运用1,1-二苯基-2-三硝基苯肼(DPPH)法评价山莓叶挥发油清除自由基能力,刃天青96孔板微量稀释法测试五种供试菌的抑菌作用。结果:鉴定山莓叶挥发油19种成分,占挥发油总质量的87.54%,其中酚类及含氧衍生物63.55%,烃类19.79%,酯类2.54%,醛类1.66%。山莓叶挥发油对DPPH自由基的清除呈量效关系,但效果弱于芦丁和Vc,对大肠杆菌、金黄色葡萄球菌、苏云金杆菌、沙门氏菌、痢疾志贺氏菌等五种供试菌均有抑菌作用,其中对痢疾志贺杆菌MIC为15.6μg/mL,效果明显好于卡拉霉素标准品。结论:山莓叶挥发油体外抗氧化能力相对较弱,对痢疾志贺杆菌抑制作用显著。
Object The chemical composition, antioxidant and antimicrobial activities of the volatile oil of Rubus corchorifolius L.f. leaves were investigated. Method The volatile oil was extracted by steam distillation, identified by GC/MS and determined by peak area normalization method. The antioxidant activity of the oil was tested by 1,1-diphenyl-2-picryl-hydrazyl(DPPH)method, the antibacterial activity was evaluated by renzurin microplate assay. Result A total of 19 components were identified, the main constituents were phenol derivatives(63.55%), alkanes(19.79%), esters(2.54%), aldehydes(1.66%)and etc. The volatile oil revealed a certain scavenging effect on DPPH free radical, the clearance effect was weaker than rutin and Vc. The volatile oil had a certain antibacterial effect on escherichia coli, staphylococcus aureus, bacillus thuring-iensis,salmonella,shigella dusenterlea. Minimum inhibitory concentration to shigella dusenterlea was 15.6 μg/mL, that is lower than concentration of kalafungin standard.Conclusion volatile oil from Rubus corchorifolius L.f. leaves showed weak antioxidant and certain inhibition effect on shigella dusenterlea.
Object The chemical composition, antioxidant and antimicrobial activities of the volatile oil of Rubus corchorifolius L.f. leaves were investigated. Method The volatile oil was extracted by steam distillation, identified by GC/MS and determined by peak area normalization method. The antioxidant activity of the oil was tested by 1,1-diphenyl-2-picryl-hydrazyl(DPPH)method, the antibacterial activity was evaluated by renzurin microplate assay. Result A total of 19 components were identified, the main constituents were phenol derivatives(63.55%), alkanes(19.79%), esters(2.54%), aldehydes(1.66%)and etc. The volatile oil revealed a certain scavenging effect on DPPH free radical, the clearance effect was weaker than rutin and Vc. The volatile oil had a certain antibacterial effect on escherichia coli, staphylococcus aureus, bacillus thuring-iensis,salmonella,shigella dusenterlea. Minimum inhibitory concentration to shigella dusenterlea was 15.6 μg/mL, that is lower than concentration of kalafungin standard.Conclusion volatile oil from Rubus corchorifolius L.f. leaves showed weak antioxidant and certain inhibition effect on shigella dusenterlea.
引文
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[13] 刘平怀,刘洋洋,陈德力,等.ASE 联合微孔板刃天青显色法快速筛选海洋抗菌活性物质[J].中国实验方剂学杂志,2010,16(15):176-178.
[2] 王世清,潘文刚,张志勇.苗族药三月泡的品种鉴定和成分研究[J].中国民族民间医药,2002(1):42-44.
[3] 湖南食品药品监督管理局编.湖南省中药材标准[M].2009版.长沙:湖南科技出版社,2010:286.
[4] 董振生,李丹,程天印,等.山莓叶药用价值研究现状及前景分析[J].现代中药研究与实践,2008,22(1):60-62.
[5] 李蕾 ,孙美利 ,张舒媛.悬钩子属药用植物活性作用及临床应用研究进展[J].中国医药导报,2014,20(8):92-94.
[6] 陈炳化,余望.山莓资源开发及其利用[ J],资源开发与市场,2000,16(5):286-289.
[7] 傅正生,杨爱梅,梁卫东.悬钩子属植物化学成分及生物活性研究进展[J].天然产物研究与开发,2001,13(5):86-90.
[8] 周丽萍,马艳芳,刘敏,等.山莓的化学成分及抗腹泻药理学研究进展[J].广州化工,2015,46(11):35-36.
[9] 周双德,陈雪香.肖苏尧山莓叶挥发油成分分析及抑菌活性研究[J].中药材,2009,32(10):1547-1549.
[10] 程恰,程天印.山莓叶挥发油化学成分的分析[J].天然产物研究与开发,2014,26(4):558-560.
[11] 欧阳婷,黄星雨 ,袁佳敏,等.不同产地香茅挥发油抑菌及抗氧化活性研究 [ J].天然产物研究与开发,2018,30(12):65-72.
[12] 张彬,邓樱花,苏明伟,等.侧蒿挥发油化学成分及抗氧化、抑菌活性研究 [ J].华中师范大学学报(自然科学版),2017,51(6):777-779.
[13] 刘平怀,刘洋洋,陈德力,等.ASE 联合微孔板刃天青显色法快速筛选海洋抗菌活性物质[J].中国实验方剂学杂志,2010,16(15):176-178.