基于HPLC结合化学计量学的壮瑶药小槐花指纹图谱研究
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摘要
目的建立基于HPLC结合化学计量学的壮瑶药小槐花指纹图谱,为其质量控制提供科学的评价方法。方法采用HPLC法,色谱柱为ZORBAX SB-C18(5μm,4.6mm×250mm),检测波长为330nm,以甲醇-0.2%磷酸水溶液梯度洗脱,流速为1.0mL/min,通过中药色谱指纹图谱相似度评价结合聚类分析和主成分分析对10批次小槐花样品进行研究。结果建立了小槐花HPLC指纹图谱,标定出13个共有峰,指纹图谱相似度均在0.9以上,聚类分析将小槐花样品大致分为4类,与主成分分析结果基本一致,影响药材质量差异是多成分协同作用的结果。结论将小槐花指纹图谱采用HPLC结合化学计量学进行分析,方法专属性强,精密度、稳定性及重现性良好,可作为小槐花质量评价和控制的有效方法。
OBJECTIVE To establish a fingerprint of Desmodium caudatumbased on HPLC and chemometrics,and to provide a scientific evaluation method for its quality control.METHODS A HPLC method was developed for the determination of10 batches of Desmodium caudatum by using ZORBAX SB-C18(5μm,4.6 mm×250 mm)column with a detection wavelength of 330 nm and gradient elution with methanol-0.2% phosphoric acid solution at a flow rate of 1.0 mL/min.RESULTS The HPLC fingerprint of Desmodium caudatum was established,13 common peaks were calibrated,and the similarity of fingerprint was above 0.9.Cluster analysis classified the samples into 4 categories,which was basically consistent with the results of principal component analysis.The effect of the quality difference of Desmodium caudatum was the result of the synergistic action of multi-components.CONCLUSION The fingerprint of Desmodium caudatum was analyzed by HPLC combined with chemometrics.The method has strong specificity,good precision,stability and reproducibility.It can be used as an effective method for quality evaluation and control of Desmodium caudatum.
OBJECTIVE To establish a fingerprint of Desmodium caudatumbased on HPLC and chemometrics,and to provide a scientific evaluation method for its quality control.METHODS A HPLC method was developed for the determination of10 batches of Desmodium caudatum by using ZORBAX SB-C18(5μm,4.6 mm×250 mm)column with a detection wavelength of 330 nm and gradient elution with methanol-0.2% phosphoric acid solution at a flow rate of 1.0 mL/min.RESULTS The HPLC fingerprint of Desmodium caudatum was established,13 common peaks were calibrated,and the similarity of fingerprint was above 0.9.Cluster analysis classified the samples into 4 categories,which was basically consistent with the results of principal component analysis.The effect of the quality difference of Desmodium caudatum was the result of the synergistic action of multi-components.CONCLUSION The fingerprint of Desmodium caudatum was analyzed by HPLC combined with chemometrics.The method has strong specificity,good precision,stability and reproducibility.It can be used as an effective method for quality evaluation and control of Desmodium caudatum.
引文
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[11]严宝飞,朱邵晴,宿树兰,等.不同产地黄芩茎叶UPLC指纹图谱与化学模式识别研究[J].南京中医药大学学报,2017,11(33):633-638.
[12]刘吉成,卢森华,谢巍,等.广西多叶越南槐HPLC指纹图谱的建立及与山豆根HPLC指纹图谱一致性比较研究[J].中国实验方剂学杂志,2014,4(1):117-119.
[13]聂韡,朱培林,房海灵,等.HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J].中草药,2017,48(1):185-191.
[14]汤丽娟,吴皓,郁红礼.大腹皮的HPLC指纹图谱研究[J].南京中医药大学学报,2014,30(3):276-279.
[15]陆金兰,朱星宇,刘玲,等.中药炮制辅料米醋的指纹图谱研究[J].南京中医药大学学报,2017,33(5):463-469.
[2]广西壮族自治区食品药品监督管理局.广西壮族自治区壮药材质量标准汇编(第一卷)[S].南宁:广西科学技术出版社,2008:49.
[3]覃迅云,罗金裕,高志刚.中国瑶药学[M].北京:民族出版社,2002:934.
[4]卢文杰,陆国寿,谭晓,等.壮瑶药小槐花化学成分研究[J].中药材,2013,36(12):1953-1956.
[5]朱丹,王帝,王光辉,等.小槐花大极性部位的化学成分研究[J].中国中药杂志,2014,39(16):3112-3116.
[6]吴瑶,罗强,孙翠玲,等.小槐花的化学成分研究[J].中国中药杂志,2012,37(12):1788-1792.
[7]陆国寿,谭晓,陈家源,等.小槐花中的脂溶性成分分析[J].广西科学,2012,19(4):355-357.
[8]李燕婧,钟正贤,卢文杰,等.小槐花醇提物药理作用研究[J].云南中医中药杂志,2013,34(5):64-65.
[9]李燕婧,钟正贤,卢文杰,等.小槐花水提物药理作用研究[J].中医药导报,2013,19(4):76-78.
[10]刘超,王俊霞,顾雪竹,等.小槐花提取物对α?葡萄糖苷酶抑制活性研究[J].中国实验方剂学杂志,2013,19(20):91-93.
[11]严宝飞,朱邵晴,宿树兰,等.不同产地黄芩茎叶UPLC指纹图谱与化学模式识别研究[J].南京中医药大学学报,2017,11(33):633-638.
[12]刘吉成,卢森华,谢巍,等.广西多叶越南槐HPLC指纹图谱的建立及与山豆根HPLC指纹图谱一致性比较研究[J].中国实验方剂学杂志,2014,4(1):117-119.
[13]聂韡,朱培林,房海灵,等.HPLC指纹图谱结合化学计量学评价不同产地广东紫珠药材的质量[J].中草药,2017,48(1):185-191.
[14]汤丽娟,吴皓,郁红礼.大腹皮的HPLC指纹图谱研究[J].南京中医药大学学报,2014,30(3):276-279.
[15]陆金兰,朱星宇,刘玲,等.中药炮制辅料米醋的指纹图谱研究[J].南京中医药大学学报,2017,33(5):463-469.