棉花竹试管快繁技术研究
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摘要
以棉花竹(Fargesia fungosa)成熟种胚诱导出的芽作为材料,研究其快速繁殖技术。结果表明:棉花竹的最佳增殖培养基为MS(Murashige and Skoog)+3 mg/L BA(6-苄基腺嘌呤),增殖系数为3.77,芽丛生长旺盛;最佳生根培养基为1/2MS+3 mg/L IBA(吲哚丁酸),生根率达100%,根系较长且粗壮;试管苗移栽至泥炭、蛭石、珍珠岩配制比例为1∶1∶1的混合基质中,成活率高达98%。本研究结果为棉花竹的扩繁和产业化生产提供了技术支撑,对箭竹属其他竹种的组织培养具有重要的参考价值。
The buds induced from mature embryos were used as explants to study the rapid propagation technique for Fargesia fungosa.Murashige and Skoog's(MS) medium supplemented with 3 mg/L BA was the optimal medium for bud multiplication.The highest proliferation coefficient was 3.77,and the cluster buds grown well.The suitable rooting medium was 1/2 MS supplemented with 3 mg/L IBA.The rooting rate was up to 100%,and the roots were long and thick.The survival rate of hardening plantlets was 98% after being transferred to a mixture of peat,vermiculite and perlite with a ratio at 1:1:1.The study provides technical support for the propagation and industrialization production of Fargesia fungosa,and also is of important reference value to the tissue culture of other species in the genus.
The buds induced from mature embryos were used as explants to study the rapid propagation technique for Fargesia fungosa.Murashige and Skoog's(MS) medium supplemented with 3 mg/L BA was the optimal medium for bud multiplication.The highest proliferation coefficient was 3.77,and the cluster buds grown well.The suitable rooting medium was 1/2 MS supplemented with 3 mg/L IBA.The rooting rate was up to 100%,and the roots were long and thick.The survival rate of hardening plantlets was 98% after being transferred to a mixture of peat,vermiculite and perlite with a ratio at 1:1:1.The study provides technical support for the propagation and industrialization production of Fargesia fungosa,and also is of important reference value to the tissue culture of other species in the genus.
引文
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[3]张春霞,谢寅峰,张幼法,等.竹子组织培养研究的进展及应用前景[J].竹子研究汇刊,1999,18(3):46-49.
[4]张春霞,王福升,黄月英.菲白竹组培繁殖技术研究[J].林业科技开发,2006,20(5):31-33.
[5]张有珍,刘倩倩,何冬冬,等.铺地竹试管快速繁殖研究[J].浙江农林大学学报,2012,29(1):151-154.
[6]王光萍,黄敏仁.日本平安竹的组织培养及快速繁殖[J].植物生理学通讯,2002,38(1):1.
[7]张铁,万京.勃氏甜龙竹的组培快繁[J].云南民族大学学报,2004,13(3):203-206.
[8]苏海,钟明,蔡时可,等.马来甜龙竹的组织培养和快速繁殖[J].植物生理学通讯,2004,40(4):468.
[9]何巨擘,程治英,郭振华,等.小叶龙竹的快速繁殖与离体保存[J].云南农业大学学报,2011,23(3):359-363.
[10]张桂和.麻竹茎尖培养及离体快繁研究[J].海南大学学报:自然科学版,1997,15(4):298-303.
[11]杨本鹏,昝丽梅.龙竹的组织培养[J].热带作物学报,2003,24(3):82-87.
[12]Sanjay Saxena.In vitro propagation of bamboo(Bambusa tulda Roxb.)through shoot proliferation[J].Plant Cell Reports,1990,9:431-434.
[13]裴海燕,方伟,林新春,等.花叶花秆绿竹的试管快繁研究[J].浙江林学院学报,2010,27(1):149-154.
[14]陈懿涵,桂仁意,林新春,等.花秆绿竹试管快速繁殖[J].浙江林学院学报,2008,25(3):397-400.
[15]Lin C S,Chang W C.Micropropagation of Bambusa edulis through nodal explants of field-grown culms and flowering of re-generated plantlets[J].Plant Cell Reports,1998,17:617-620.
[16]徐强兴,杨广超.吊丝球竹的组培快繁技术研究[J].广东农业科学,2007,2:42-44.
[17]张光楚,王裕霞,谭源杰,等.丛生竹的组培快繁技术[J].竹子研究汇刊,2004,23(1):13-20.
[18]王光萍,丁雨龙.几种观赏竹种组织培养研究[J].竹子研究汇刊,2002,21(4):5-9.
[19]李睿,章笕,章珠娥.中国竹类植物生物多样性的价值及保护进展[J].竹子研究汇刊,2003,22(4):7-12.
[20]陈锐亮,杨振德.壮绿竹茎段培养的初步研究[J].广西热作科技,1999,3:20-21.