铅、铜复合胁迫对木豆幼苗生长和生理特性的影响
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摘要
通过盆栽法,研究了铅(Pb)、铜(Cu)复合胁迫对木豆(Cajanus cajan)幼苗生长和生理特性的影响,探讨木豆是否适合用于修复铅、铜重金属污染的土壤。研究结果表明,处理5:25 mg/L (Pb)+100 mg/L(Cu)下,木豆的株高、根长较对照相比显著下降;所有处理下,叶绿素a含量差异并不显著,处理4:10 mg/L(Pb)+50 mg/L (Cu)下,叶绿素b的含量显著高于对照,类胡萝卜素含量最低;对照的电导率低于其他四个处理,但差异并不显著;处理5下木豆地上部分的SOD的活性降到最低,MDA的含量增加到最大,地上部和地下部GSH的含量均达到最低。低浓度铅、铜复合胁迫下,木豆的生长受到了促进作用,但浓度过高时,便对木豆产生了毒害。木豆可以用于铅、铜污染土壤的修复。
The effects of lead(Pb), copper(Cu)compound stress on the growth and physiological characteristics of(Cajanus cajan) seedlings were studied by pot experiment. The results showed that under 5:25mg/L(Pb) 100mg/L(Cu) treatment, the plant height and root length decreased significantly, but there was no significant difference in chlorophyll a content under all treatments, but the chlorophyll b content in treatment 4:10mg/L(Pb) 50mg/L(Cu) was significantly higher than that in control. Carotenoid The electrical conductivity of the control was lower than that of the other four treatments, but the difference was not significant. The activity of SOD decreased to the lowest, the content of MDA increased to the maximum, and the GSH content of both the aboveground part and the underground part reached the lowest in treatment 5. Under low concentration of lead and copper, the growth of woody bean was promoted, but when the concentration was too high, it was toxic to the bean. Wood beans can be used for remediation of lead and copper contaminated soils.
The effects of lead(Pb), copper(Cu)compound stress on the growth and physiological characteristics of(Cajanus cajan) seedlings were studied by pot experiment. The results showed that under 5:25mg/L(Pb) 100mg/L(Cu) treatment, the plant height and root length decreased significantly, but there was no significant difference in chlorophyll a content under all treatments, but the chlorophyll b content in treatment 4:10mg/L(Pb) 50mg/L(Cu) was significantly higher than that in control. Carotenoid The electrical conductivity of the control was lower than that of the other four treatments, but the difference was not significant. The activity of SOD decreased to the lowest, the content of MDA increased to the maximum, and the GSH content of both the aboveground part and the underground part reached the lowest in treatment 5. Under low concentration of lead and copper, the growth of woody bean was promoted, but when the concentration was too high, it was toxic to the bean. Wood beans can be used for remediation of lead and copper contaminated soils.
引文
[1]韩玉林.Pb-Cu复合胁迫对马蔺幼苗生长和生理指标的影响[J].植物资源与环境学报,2010,1919(4):24-30.
[2]周东美,郝秀珍,薛艳,等.污染土壤的修复技术研究进展[J].生态环境,2004,13(2):234-242.
[3]王瑞.豆科植物对铜尾矿砂的耐性筛选及其修复潜力研究[D].南昌:江西财经大学,2018.
[4]王沿沿.铝毒胁迫下不同耐性的木豆(Cajanus cajan(L.)Millsp)品种根结构和光合特性的差异比较[D].南宁:广西大学,2014.
[5]高扬,毛亮,周培,等.Pb、Cd复合胁迫下4种植物抗氧化防御差异性研究[J].中国生态农业学报,2010,18(4):836-842.
[6]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[7] Ma F W,Cheng L L.The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate-glutathione pathay than the shaded peel[J].Plant Science,2003,165(4):819-827.
[8]潘雪峰,李明,赵盼,等.铜胁迫对穿心莲幼苗生长及生理特性的影响[J].植物科学学报,2015,33(02):218-225.
[9]史吉平,董永华.重金属胁边对小麦幼苗超氧化物歧化酶活性的影响[J].麦类作物学报1996(3):33-34.
[10]吕新民,杨怡帆,鲁晓燕,等.NaCl胁迫对酸枣幼苗AsA-GSH循环的影响[J].植物生理学报,2016,52(05):736-744.
[2]周东美,郝秀珍,薛艳,等.污染土壤的修复技术研究进展[J].生态环境,2004,13(2):234-242.
[3]王瑞.豆科植物对铜尾矿砂的耐性筛选及其修复潜力研究[D].南昌:江西财经大学,2018.
[4]王沿沿.铝毒胁迫下不同耐性的木豆(Cajanus cajan(L.)Millsp)品种根结构和光合特性的差异比较[D].南宁:广西大学,2014.
[5]高扬,毛亮,周培,等.Pb、Cd复合胁迫下4种植物抗氧化防御差异性研究[J].中国生态农业学报,2010,18(4):836-842.
[6]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[7] Ma F W,Cheng L L.The sun-exposed peel of apple fruit has higher xanthophyll cycle-dependent thermal dissipation and antioxidants of the ascorbate-glutathione pathay than the shaded peel[J].Plant Science,2003,165(4):819-827.
[8]潘雪峰,李明,赵盼,等.铜胁迫对穿心莲幼苗生长及生理特性的影响[J].植物科学学报,2015,33(02):218-225.
[9]史吉平,董永华.重金属胁边对小麦幼苗超氧化物歧化酶活性的影响[J].麦类作物学报1996(3):33-34.
[10]吕新民,杨怡帆,鲁晓燕,等.NaCl胁迫对酸枣幼苗AsA-GSH循环的影响[J].植物生理学报,2016,52(05):736-744.