复合植物精油对脂多糖刺激仔猪肝脏的保护作用
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摘要
试验旨在研究复合植物精油(OCT)对脂多糖(LPS)刺激仔猪肝脏的保护作用。选取18头仔猪,随机分为对照组、LPS组和OCT+LPS组,每组6个重复。对照组与LPS组饲喂基础日粮,OCT+LPS组在基础日粮中添加50 mg/kg OCT。试验期21 d。于试验第21天,LPS组与OCT+LPS组仔猪腹腔注射LPS,对照组注射等量的生理盐水,3 h后采血,6 h后屠宰。结果表明:与对照组相比,LPS刺激提高了血浆谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷酰转肽酶(GGT)、肝脏诱导型一氧化氮合酶(i-NOS)的活力(P<0.05),肝脏热休克蛋白70(HSP70)基因的相对表达量显著提高(P<0.05);仔猪肝脏表皮生长因子(EGF)、白细胞介素10(IL-10)、胰岛素样因子(IGF-1)、丝氨酸蛋白激酶(mTOR)基因的相对表达量显著降低(P<0.05)。在日粮中添加50 mg/kg的OCT,可缓解LPS导致的仔猪血浆ALT、AST、GGT活力的升高以及肝脏i-NOS活力的升高(P<0.05),且有缓解LPS导致的仔猪肝脏MPO活力升高的趋势(P<0.1),另外可缓解LPS导致的仔猪肝脏HSP70基因相对表达量的升高以及EGF、IL-10、IGF-1、mTOR等基因相对表达量的降低(P<0.05)。综上可知,日粮中添加50 mg/kg的OCT可缓解LPS刺激引起的肝脏炎症反应,提高仔猪肝脏细胞的生长和增殖,进而缓解LPS刺激导致的仔猪肝脏氧化应激损伤。
This study was conducted to determine whether the essential oil compound(OCT) could alleviate the liver injury of lipopolysaccharide(LPS)-challenged piglets. Eighteen crossbred healthy piglets were randomly assigned into the control, LPS, and OCT+LPS groups. Six pigs in every group. Piglets in the control and LPS groups were fed with the basal diet, whereas the OCT+LPS group fed the basal diet supplemented with 50 mg/kg OCT. The experiment lasted for 21 days. On day 21 of the trial, Piglets in the LPS and OCT+LPS groups were intraperitoneal administration of LPS, while the control pigs received the same volume of saline. Blood was collected from jugular vein at 3 h post LPS or saline injection, and all pigs were killed to obtain the liver at 6 h post LPS or saline injection. The results showed that: The LPS challenge increased the activities of plasma ALT, AST, ALP, GGT and hepatic i NOS activity(P<0.05). Additionally, LPS increased the m RNA level for HSP70, while decreased the m RNA levels for EGF, IL-10, IGF-1, and mTOR in the liver of piglets(P<0.05). With LPS stimulation, 50 mg/kg OCT supplementation alleviated the increase of plasma ALT, AST, ALP, GGT and hepatic i NOS activities(P<0.05). alleviated the uptence of the hepatic MPO activity(P <0.1). Beyond that, 50 mg/kg OCT supplementation alleviated the increase of the m RNA level for HSP70 and the decrease of the m RNA levels for EGF, IL-10, IGF-1, mTOR in the liver with LPS stimulation(P<0.05). These results suggest that dietary supplementation with 50 mg/kg OCT could alleviate liver inflammation induced by LPS stimulation, improve the growth and proliferation of liver cells, thus alleviate the oxidative stress injury of piglets caused by LPS stimulation.
This study was conducted to determine whether the essential oil compound(OCT) could alleviate the liver injury of lipopolysaccharide(LPS)-challenged piglets. Eighteen crossbred healthy piglets were randomly assigned into the control, LPS, and OCT+LPS groups. Six pigs in every group. Piglets in the control and LPS groups were fed with the basal diet, whereas the OCT+LPS group fed the basal diet supplemented with 50 mg/kg OCT. The experiment lasted for 21 days. On day 21 of the trial, Piglets in the LPS and OCT+LPS groups were intraperitoneal administration of LPS, while the control pigs received the same volume of saline. Blood was collected from jugular vein at 3 h post LPS or saline injection, and all pigs were killed to obtain the liver at 6 h post LPS or saline injection. The results showed that: The LPS challenge increased the activities of plasma ALT, AST, ALP, GGT and hepatic i NOS activity(P<0.05). Additionally, LPS increased the m RNA level for HSP70, while decreased the m RNA levels for EGF, IL-10, IGF-1, and mTOR in the liver of piglets(P<0.05). With LPS stimulation, 50 mg/kg OCT supplementation alleviated the increase of plasma ALT, AST, ALP, GGT and hepatic i NOS activities(P<0.05). alleviated the uptence of the hepatic MPO activity(P <0.1). Beyond that, 50 mg/kg OCT supplementation alleviated the increase of the m RNA level for HSP70 and the decrease of the m RNA levels for EGF, IL-10, IGF-1, mTOR in the liver with LPS stimulation(P<0.05). These results suggest that dietary supplementation with 50 mg/kg OCT could alleviate liver inflammation induced by LPS stimulation, improve the growth and proliferation of liver cells, thus alleviate the oxidative stress injury of piglets caused by LPS stimulation.
引文
[1]刘玉兰.仔猪免疫应激及其营养调控研究进展[J].中国畜牧杂志,2017,53(10):1-3,11.
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[11]Collins T F X,Sprando R L,Black T N,et al.Effects of zearalenone on in utero development in rats[J].Food Chem Toxicol,2006,44(9):1455-1465.
[12]张弛,刘瑛,李华珠,等.血清谷丙转氨酶在正常范围内对非酒精性脂肪性肝病的预测[J].世界华人消化杂志,2011,19(8):841 844.
[13]姜晓丹,小林俊博,宋文光,等.脂多糖诱发急性肺炎时中性粒细胞碱性磷酸酶细胞化学定位观察[J].解剖学报,2000,31(1):52-55.
[14]朱陈,孙源源,金燕.血清甲胎蛋白、γ-谷氨酰转肽酶、高尔基体蛋白73检测在原发性肝癌早期诊断中的价值[J].临床肝胆病杂志,2014,31(10):1064.
[15]Wang L,Hou Y Q,Yi D,et al.Beneficial roles of dietary oleum cinnamomi in alleviating intestinal injury[J].Front Biosci,2015,20(5):814-828.
[16]陈柏荣,危小良,彭湖,等.髓过氧化物酶与冠状动脉慢血流的相关性[J].中国动脉硬化杂志,2015,23(2):185-187.
[17]张伟.N-乙酰半胱氨酸对脂多糖刺激仔猪肠道屏障功能及抗氧化能力的影响[D].武汉:武汉轻工大学,2011.
[18]张伟,杨震国,侯永清,等.N-乙酰半胱氨酸对脂多糖刺激仔猪空肠黏膜抗氧化能力的影响[J].动物营养学报,2011,23(5):842-847.
[19]Ozbayer C,Kurt H,Ozdemir Z,et al.Gastroprotective,cytoprotective and antioxidant effects of Oleum cinnamomi on ethanol induced damage[J].Cytotechnology,2014,66(3):431-441.
[20]程维杰,李秋玲,孙延鸣,等.热休克蛋白70(HSP70)研究进展[J].畜牧兽医杂志,2008,2(6):55-57.
[21]Yi D,Hou Y Q,Wang L,et al.Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharidechallenged piglets[J].Brit J Nutr,2014,111:46-54.
[22]Hou Y Q,Wang L,Yi D,et al.N-acetylcysteine reduces inflammation in the small intestine by regulating redox,EGFand TLR4 signaling[J].Amino Acids,2013,45:513-522.
[23]席全胜,钱欣国,甘人宝.EGF家族研究进展[J].生命化学,1999,19(3):135-137.
[24]李枚涓,王琰,孔艳.白介素10在粥样斑块形成机制中的研究进展[J].心血管进展,2009(30):11-16.
[25]高萍,傅伟龙,朱晓丹,等.蓝塘仔猪IGF-1水平与组织IGF-1、GHR基因的表达[J].畜牧兽医学报,2005,36(1):38-42.
[26]潘智,张令强,蒋继志,等.mTOR的研究进展[J].细胞生物学杂志,2006(28):395-398.
[2]Brence A,Roura E.Essential oil in poultry nutrition:Main effects and modes of action[J].Anim Feed Sci Tech,2010,158(1):1-14.
[3]宋洪涛,郭涛,颜秀涛,等.肉桂油β-环糊精包合物的制备工艺研究[J].中草药,2000,31(11):818-820.
[4]邱楚武.牛至油在仔猪饲料中的应用试验[J].粮食与饲料工业,2003(7):32-33.
[5]王梓贞,李琪.百里香精油化学成分分析及其抗菌、抗氧化活性的研究进展[J].特种经济动植物,2013,16(8):35-36.
[6]中国香料香精化妆品协会.欧盟批准百里香酚用作植保产品[J].国内外香化信息,2013(7):518.
[7]Schmocker C,Weylandt K H,Kahlke L,et al.Omega-3fatty acids alleviate chemically induced acute hepatitis by suppression of cytokines[J].Hepatology,2007,45(4):864-869.
[8]汪中兴,侯永清,丁斌鹰,等.复合植物精油对脂多糖刺激断奶仔猪生长性能和生长相关基因m RNA表达的影响[A].第七届中国饲料营养学术研讨会论文集[C].郑州:中国畜牧兽医学会动物营养学分会,2014.
[9]Fu WJ,Stromberg A J,Viele K,et al.Statistics and bioinformatics in nutritional sciences:analysis of complex date in the era of systems biology[J].J Nutr Biochem,2010,21(7):561-572.
[10]Liu YL,Li D F,Gong L M,et al.Effects of fish oil supplementation on the performance and the immunological,adrenal,and somatotropic responses of weaned pigs after an Escherichia coli lipopolysaccharide challenge[J].J Anim Sci,2003,81:2758-2765.
[11]Collins T F X,Sprando R L,Black T N,et al.Effects of zearalenone on in utero development in rats[J].Food Chem Toxicol,2006,44(9):1455-1465.
[12]张弛,刘瑛,李华珠,等.血清谷丙转氨酶在正常范围内对非酒精性脂肪性肝病的预测[J].世界华人消化杂志,2011,19(8):841 844.
[13]姜晓丹,小林俊博,宋文光,等.脂多糖诱发急性肺炎时中性粒细胞碱性磷酸酶细胞化学定位观察[J].解剖学报,2000,31(1):52-55.
[14]朱陈,孙源源,金燕.血清甲胎蛋白、γ-谷氨酰转肽酶、高尔基体蛋白73检测在原发性肝癌早期诊断中的价值[J].临床肝胆病杂志,2014,31(10):1064.
[15]Wang L,Hou Y Q,Yi D,et al.Beneficial roles of dietary oleum cinnamomi in alleviating intestinal injury[J].Front Biosci,2015,20(5):814-828.
[16]陈柏荣,危小良,彭湖,等.髓过氧化物酶与冠状动脉慢血流的相关性[J].中国动脉硬化杂志,2015,23(2):185-187.
[17]张伟.N-乙酰半胱氨酸对脂多糖刺激仔猪肠道屏障功能及抗氧化能力的影响[D].武汉:武汉轻工大学,2011.
[18]张伟,杨震国,侯永清,等.N-乙酰半胱氨酸对脂多糖刺激仔猪空肠黏膜抗氧化能力的影响[J].动物营养学报,2011,23(5):842-847.
[19]Ozbayer C,Kurt H,Ozdemir Z,et al.Gastroprotective,cytoprotective and antioxidant effects of Oleum cinnamomi on ethanol induced damage[J].Cytotechnology,2014,66(3):431-441.
[20]程维杰,李秋玲,孙延鸣,等.热休克蛋白70(HSP70)研究进展[J].畜牧兽医杂志,2008,2(6):55-57.
[21]Yi D,Hou Y Q,Wang L,et al.Dietary N-acetylcysteine supplementation alleviates liver injury in lipopolysaccharidechallenged piglets[J].Brit J Nutr,2014,111:46-54.
[22]Hou Y Q,Wang L,Yi D,et al.N-acetylcysteine reduces inflammation in the small intestine by regulating redox,EGFand TLR4 signaling[J].Amino Acids,2013,45:513-522.
[23]席全胜,钱欣国,甘人宝.EGF家族研究进展[J].生命化学,1999,19(3):135-137.
[24]李枚涓,王琰,孔艳.白介素10在粥样斑块形成机制中的研究进展[J].心血管进展,2009(30):11-16.
[25]高萍,傅伟龙,朱晓丹,等.蓝塘仔猪IGF-1水平与组织IGF-1、GHR基因的表达[J].畜牧兽医学报,2005,36(1):38-42.
[26]潘智,张令强,蒋继志,等.mTOR的研究进展[J].细胞生物学杂志,2006(28):395-398.