基于标准汤剂参比的二冬汤颗粒提取工艺研究
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摘要
目的:确定二冬汤颗粒的最佳提取工艺。方法:以二冬汤标准汤剂为参比,以黄芩苷、甘草酸、总皂苷、总多糖、浸膏率、乙醇可溶物、正丁醇可溶物为指标,采用正交试验考察加水量、浸泡时间、煎煮时间对提取效果的影响,确定二冬汤的最佳提取工艺。结果:最佳提取工艺为药材饮片加水煎煮2次,第一次加10倍量水,煎煮2 h,第二次加8倍量水,煎煮1 h。提取液中各成分与标准汤液中相应成分的比值分别为:黄芩苷1.54、甘草酸1.42、总皂苷1.51、总多糖1.45、浸膏率1.53、乙醇可溶物1.61、正丁醇可溶物1.52,比值相近,平均1.51,变异系数3.18%。结论:该提取工艺稳定可行,适于工业化生产。
Objective:To determine the optimal extraction process of Erdongtang Granules. Methods:Taking Erdong standard decoction as reference,using baicalin,glycyrrhizic acid,total saponin,total polysaccharide,extract rate,ethanol soluble matter and n-butanol soluble matter as indicators,orthogonal test was used to investigate the effects of adding water,soaking time and boiling time on the extraction and the best extraction process of Erdong Decoction were determined. Results:The best extraction process was to boil the medicinal herbs with water for 2 times,add 10 times the amount of water for the first time,boiling for 2 hours,add 8 times the amount of water for the second time,and boiling for 1 hour. The ratio of each component in the extract to the corresponding components in the standard decoction was:baicalin 1.54,glycyrrhizic acid 1.42,total saponin 1.51,total polysaccharide 1.45,extract rate 1.53,ethanol soluble matter 1.61,n-butanol soluble matter 1.52,the ratio is similar,with an average of 1.51 and a coefficient of variation of 3.18%. Conclusion:The extraction process is stable and feasible,suitable for industrial production.
Objective:To determine the optimal extraction process of Erdongtang Granules. Methods:Taking Erdong standard decoction as reference,using baicalin,glycyrrhizic acid,total saponin,total polysaccharide,extract rate,ethanol soluble matter and n-butanol soluble matter as indicators,orthogonal test was used to investigate the effects of adding water,soaking time and boiling time on the extraction and the best extraction process of Erdong Decoction were determined. Results:The best extraction process was to boil the medicinal herbs with water for 2 times,add 10 times the amount of water for the first time,boiling for 2 hours,add 8 times the amount of water for the second time,and boiling for 1 hour. The ratio of each component in the extract to the corresponding components in the standard decoction was:baicalin 1.54,glycyrrhizic acid 1.42,total saponin 1.51,total polysaccharide 1.45,extract rate 1.53,ethanol soluble matter 1.61,n-butanol soluble matter 1.52,the ratio is similar,with an average of 1.51 and a coefficient of variation of 3.18%. Conclusion:The extraction process is stable and feasible,suitable for industrial production.
引文
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[8]林启焰,谢凡,程雪梅,等.正交试验优化滋肾养阴颗粒剂提取工艺[J].中成药,2017,39(1):213-215.
[9]盛华刚,朱立俏,林桂涛.壳聚糖澄清剂对枳实薤白桂枝汤颗粒提取液的纯化工艺研究[J].中国实验方剂学杂志,2010,16(4):15-17.
[2]李翔,刘皈阳.高效液相色谱法测定芩玄颗粒中黄芩苷含量[J].中国实验方剂学杂志,2013,19(3):110-112.
[3]唐慧慧,蔡清宇,张丽娟. HPLC测定小儿清肺化痰颗粒中黄芩苷的含量[J].中国实验方剂学杂志,2012,18(21):112-114.
[4]孟蕾,曹杰,张海鸣,等. HPLC法测定铁笛丸中甘草酸的含量[J].中国实验方剂学杂志,2010,16(4):35-36.
[5]盛华刚,林桂涛,徐桂红.金蓝胶囊提取工艺的研究[J].山东中医药大学学报,2006,30(2):148-151.
[6]朱立俏,盛华刚.正交试验优选藤梨根总多糖的水提取工艺[J].广州化工,2013,41(1):82-83.
[7]边洪荣,娄桂芹,张庆波,等.黄芪桂枝五物汤不同剂量配比多糖含量测定[J].中药材,2007,30(6):729-731.
[8]林启焰,谢凡,程雪梅,等.正交试验优化滋肾养阴颗粒剂提取工艺[J].中成药,2017,39(1):213-215.
[9]盛华刚,朱立俏,林桂涛.壳聚糖澄清剂对枳实薤白桂枝汤颗粒提取液的纯化工艺研究[J].中国实验方剂学杂志,2010,16(4):15-17.