Fe_3O_4/氧化石墨烯复合纳米粒子的制备及其体外毒性评价
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摘要
用溶剂热法制备得到Fe_3O_4/氧化石墨烯复合纳米粒子,并对其生物相容性进行研究。使用X射线粉末衍射仪(XRD)、扫描电子显微镜(SEM)等分析仪器对其进行表征,并通过一系列溶血试验及MTT细胞毒性试验对复合纳米粒子的体外毒性进行评价。结果显示,当复合纳米粒子浓度小于5g/L时,溶血率低于5.00%;当复合纳米粒子浓度不高于0.25g/L、孵育时间不长于72h时,细胞相对增殖率大于75%,属0~1级,认为此范围浓度材料没有细胞毒性。从目前的实验结果来看制备所得Fe_3O_4/氧化石墨烯复合纳米粒子具有良好的生物相容性,在生物医学方面具有潜在的应用前景。
Fe_3O_4/graphene oxide(Fe_3O_4/GO)nanocomposite was synthesized by solvothermal strategy.The effects of reagent ratio and reaction time on the morphology and diameters of nanocomposites were investigated.They were characterized using X-ray diffraction,scanning electron microscopy,thermogravimetric analysis,and Fourier-transform infrared spectroscopy.Cytotoxicity test and hemolysis assay were investigated to assess their toxicity in vitro and to explore their potential application in the biomedical fields.The toxicity of Fe_3O_4/GO nanoparticles in mouse fibroblast(L-929)cell lines is between grade 0 to grade 1,and the material lacks hemolysis activity.
Fe_3O_4/graphene oxide(Fe_3O_4/GO)nanocomposite was synthesized by solvothermal strategy.The effects of reagent ratio and reaction time on the morphology and diameters of nanocomposites were investigated.They were characterized using X-ray diffraction,scanning electron microscopy,thermogravimetric analysis,and Fourier-transform infrared spectroscopy.Cytotoxicity test and hemolysis assay were investigated to assess their toxicity in vitro and to explore their potential application in the biomedical fields.The toxicity of Fe_3O_4/GO nanoparticles in mouse fibroblast(L-929)cell lines is between grade 0 to grade 1,and the material lacks hemolysis activity.
引文
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[2]LI Sheng,XIAO Ling,DENG Hongbing,et al.Remote controlled drug release from multi-functional Fe3O4/GO/Chitosan microspheres fabricated by an electrospray method[J].Colloids and Surfaces B Biointerfaces,2017,151:354-362.
[3]HAN Lihua,ZHAO Youchun,GAO Yang,et al.Preparation and drug release properties of Fe3O4/carboxymethyl chitosan magnetic drug carrying microspheres[J].Chemistry and Bioengineering,2015,32(5):33-36.
[4]MA Kun,ZHAO Hongxin,ZHENG Xinwei,et al.NMR studies of the interactions between AMB-1Mms6protein and magnetosome Fe3O4nanoparticles[J].Journal of Materials Chemistry B,2017,5:2888-2895.
[5]BOGACHEV Y V,NIKITINA A V,KOSTINA A A,et al.NMR relaxation efficiency of aqueous solutions of composite MgxZnyFe3-x-yO4nanoparticles[J].Applied Magnetic Resonance,2017,28(5):1-8.
[6]JIAN Xian,WU Biao,WE Yufeng,et al.Facile synthesis of Fe3O4/GCs composites and their enhanced microwave absorption properties[J].Acs Applied Materials and Interfaces,2016,8(9):6101-6109.
[7]XU Y,MAHMOOD M,FEJLEH A,et al.Carbon-covered magnetic nanomaterials and their application for the thermolysis of cancer cells[J].International Journal of Nanomedicine,2010,5(1):167-176.
[8]LANDGRAF L,CHRISTNER C,STORCK W,et al.A plasma protein corona enhances the biocompatibility of Au@Fe3O4Janus particles[J].Biomaterials,2015,68:77-88.
[9]GILJE S,HAN S,WANG M,et al.A chemical route to graphene for device applications[J].Nano Letters,2007,7(11):3394-3398.
[10]ZHANG Shouting,LI Hua,WANG Zhiyi,et al.A strongly coupled Au/Fe3O4/GO hybrid material with enhanced nanozyme activity for highly sensitive colorimetric detection,and rapid and efficient removal of Hg2+in aqueous solutions[J].Nanoscale,2015,7(18):8495-8502.
[11]MONDAL J,NGUYEN K T,JANA A,et al.Efficient alkene hydrogenation over a magnetically recoverable and recyclable Fe3O4@GO nanocatalyst using hydrazine hydrate as the hydrogen source[J].Chemical Communications,2014,50(81):12095-12097.
[12]JAFARIZAD A,GHARIBIAN S,KHOSROWSHAHI Y B.Synthesis of Fe3O4 nanoparticles and Fe3O4/GO nanocomposites with low effects on stem cells for cancer cell therapy[C]//Nanomaterials:Application and Properties(NAP),International Conference on.IEEE,Lviv:2016:02NABM02:1-3.DOI:10.1109/NAP.2016.7757286
[13]JOHNSON C E,JOHNSON J A,HAH H Y,et al.M9ssbauer studies of stoichiometry of Fe3O4:characterization of nanoparticles for biomedical applications[J].Hyperfine Interactions,2016,237(1):1-10.
[14]WANG Guangshuo,WEI Zhiyong,LIN Sang,et al.Magnetic graphene oxide-Fe3O4 nanocomposites with dual functional properties:drug delivery and magnetic resonance imaging[J].Journal of Controlled Release,2013,172(1):e32.
[15]WANG Guangshuo,CHEN Guangyi,WEI Zhiyong,et al.Multifunctional Fe3O4/graphene oxide nanocomposites for magnetic resonance imaging and drug delivery[J].Materials Chemistry and Physics,2013,141(2/3):997-1004.
[16]HEIDARIZADEH M,DOUSTKHAH E,ROSTAMNIA S,et al.Dithiocarbamate to modify magnetic graphene oxide nanocomposite(Fe3O4-GO):A new strategy for covalent enzyme(lipase)immobilization to fabrication a new nanobiocatalyst for enzymatic hydrolysis of PNPD[J].International Journal of Biological Macromolecules,2017,101:696-702.
[17]JR W S H,OFFEMAN R E.Preparation of graphitic oxide[J].Journal of the American Chemical Society,1958,80(6):1339-1339.
[18]张富强,佘文珺,傅远飞.六种纳米载银无机抗菌剂的体外细胞毒性比较[J].中华口腔医学杂志,2005,40(6):504-507.
[19]HE Hongkun,GAO Chao.Supraparamagnetic conductive and processable multifunctional graphene nanosheets coated with high-density Fe3O4nanoparticles[J].Acs Applied Materials and Interfaces,2010,2(11):3201-3210.
[20]ALLEN M J,TUNG V C,KANER R B.Honeycomb carbon:a review of graphene[J].Chemical Reviews,2010,110(1):132-145.
[21]SHARMA R K,PURI A,MONGA Y,et al.Acetoacetanilide-functionalized Fe3O4 nanoparticles for selective and cyclic removal of Pb2+ions from different charged wastewaters[J].Journal of Materials Chemistry A,2014,2(32):12888-12898.
[22]DU G H,LIU Z L,XIA X,et al.Characterization and application of Fe3O4/SiO2nanocomposites[J].Journal of SolGel Science and Technology,2006,39(3):285-291.
[23]ZHAO Linling,LIU Huarong,WANG Fengwei,et al.Design of yolk-shell Fe3O4@PMAA composite microspheres for adsorption of metal ions and pH-controlled drug delivery[J].Journal of Materials Chemistry A,2014,2(19):7065-7074.
[24]XIE Guoqiang,XI Pinxian,LIU Hongyan,et al.A facile chemical method to produce superparamagnetic graphene oxide-Fe3O4hybrid composite and its application in the removal of dyes from aqueous solution[J].Journal of Materials Chemistry,2011,22(3):1033-1039.