用于分离纯化组氨酸标签蛋白质的磁性复合纳米材料的制备
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摘要
采用溶剂热法制备Fe_3O_4纳米粒子,通过MPS和聚丙烯酸修饰,使其表面羧基化,再与NTA-Ni~(2+)螯合,制备Fe_3O_4/MPS/PAA/NTA-Ni~(2+)磁性复合纳米粒子。利用透射电镜、激光粒度仪、红外光谱进行表征。结果表明,Fe_3O_4/MPS/PAA/NTA-Ni~(2+)磁性复合纳米粒子的形貌为球形,且较为分散,其平均水合粒径为440 nm,Zeta电位为-15.8 mV,红外光谱证实了其化学结构。对组氨酸标签蛋白的分离能力为15.6μg蛋白质/mg磁性材料,说明此金属螯合吸附剂对组氨酸标签蛋白的选择性吸附有一定的意义。
Fe_3O_4 nanoparticles were prepared by solvent thermal method,and then functionalized by MPS and polyacrylic acid.The Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles were prepared by the chelation between Fe_3O_4/MPS/PAA and NTA-Ni~(2+).Finally,the Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles were characterized by the transmission electron microscopy,laser particle size instrument and infrared spectrum.The results showed that the morphology of Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles was spherical,and the average particle size was 440 nm with the average Zeta potential of-15.8 m V.The infrared spectrum confirmed its chemical structure.Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles could separate and purify the histidine-tagged protein easily,with the separation capacity of 15.6 μg protein/mg magnetic materials,indicating that the metal chelating adsorbent has certain significance for protein separation.
Fe_3O_4 nanoparticles were prepared by solvent thermal method,and then functionalized by MPS and polyacrylic acid.The Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles were prepared by the chelation between Fe_3O_4/MPS/PAA and NTA-Ni~(2+).Finally,the Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles were characterized by the transmission electron microscopy,laser particle size instrument and infrared spectrum.The results showed that the morphology of Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles was spherical,and the average particle size was 440 nm with the average Zeta potential of-15.8 m V.The infrared spectrum confirmed its chemical structure.Fe_3O_4/MPS/PAA/NTA-Ni~(2+)magnetic composite nanoparticles could separate and purify the histidine-tagged protein easily,with the separation capacity of 15.6 μg protein/mg magnetic materials,indicating that the metal chelating adsorbent has certain significance for protein separation.
引文
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[4]严微,郭娇娇,向晨阳,等.磁性四氧化三铁纳米粒子在医学诊疗领域的研究[J].湖北大学学报:自然科学版,2017,39(3):317-322.
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